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You searched for: 2015 (Year of publication)
Showing 1 - 50 of 784
Showing 1 - 50 of 784
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV150108 | 1/4 | Homo sapiens | MCF-7 |
(d)(U)C Filtration |
Tosar JP | 2015 | 67% | |
Study summaryFull title
All authors
Tosar JP, Gámbaro F, Sanguinetti J, Bonilla B, Witwer KW, Cayota A.
Journal
Nucleic Acids Res
Abstract
Intercellular communication can be mediated by extracellular small regulatory RNAs (sRNAs). Circulat (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: TSG101/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MCF-7
EV-harvesting Medium
Serum free medium
Cell viability (%)
95
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
150
Pelleting: rotor type
SW 40 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
7
Wash: time (min)
150
Wash: Rotor Type
SW 40 Ti
Wash: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD9/ CD63/ TSG101
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing;ddPCR
Database
Yes
Proteinase treatment
Yes
Moment of Proteinase treatment
After
Proteinase type
Proteinase K
Proteinase concentration
64
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
RNase A
RNAse concentration
4U/mL
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Not Reported
NTA
Report type
Not Reported
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
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EV150009 | 1/1 | Homo sapiens | Placental perfusate |
(d)(U)C Filtration |
Dragovic RA | 2015 | 67% | |
Study summaryFull title
All authors
Dragovic RA, Collett GP, Hole P, Ferguson DJ, Redman CW, Sargent IL, Tannetta DS
Journal
Methods
Abstract
The human placenta releases multiple types and sizes of syncytiotrophoblast (STB) extracellular vesi (show more...)
EV-METRIC
67% (91st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Placental perfusate
Sample origin
NAY
Focus vesicles
exosomes / microvesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
162 (pelleting)
Protein markers
EV: Alix/ CD63
non-EV: CD45/ CD235a/b/ CD41 Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Placental perfusate
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
TST28.39
Pelleting: adjusted k-factor
162.0
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63
Detected contaminants
CD41/ CD235a/b/ CD45
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV150004 | 1/2 | Homo sapiens | NAY |
(d)(U)C DG |
Clark DJ | 2015 | 67% | |
Study summaryFull title
All authors
Clark DJ, Fondrie WE, Liao Z, Hanson PI, Fulton A, Mao L, Yang AJ
Journal
Anal Chem
Abstract
Exosomes are microvesicles of endocytic origin constitutively released by multiple cell types into t (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Alix/ TSG101/ CD63
non-EV: Beta-actin/ Cell organelle protein Proteomics
yes
EV density (g/ml)
1.14-1.19
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
5
Highest density fraction
40
Orientation
Top-down
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ TSG101
Detected contaminants
Cell organelle protein/ Beta-actin
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV150008 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG Filtration |
Chan YK | 2015 | 67% | |
Study summaryFull title
All authors
Chan YK, Zhang H, Liu P, Tsao SW, Lung ML, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal
Int J Cancer
Abstract
Exosomes, a group of secreted extracellular nanovesicles containing genetic materials and signaling (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
156.9 (pelleting)
Protein markers
EV: CD63/ CD9
non-EV: Cell organelle protein Proteomics
yes
EV density (g/ml)
1.17-1.19
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
156.9
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.2 M
Highest density fraction
2.5 M
Orientation
Top-down
Rotor type
90Ti
Speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD9
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
TRPS
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV230654 | 1/1 | Mycobacterium tuberculosis | H37Rv |
(d)(U)C DC DG Filtration UF |
Lee J | 2015 | 63% | |
Study summaryFull title
All authors
Lee J, Kim SH, Choi DS, Lee JS, Kim DK, Go G, Park SM, Kim SH, Shin JH, Chang CL, Gho YS
Journal
Proteomics
Abstract
The release of extracellular vesicles, also known as outer membrane vesicles, membrane vesicles, exo (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Density gradient Filtration Ultrafiltration Protein markers
EV: LprG
non-EV: None Proteomics
yes
EV density (g/ml)
1,17
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Mycobacterium tuberculosis
Sample Type
Cell culture supernatant
EV-producing cells
H37Rv
EV-harvesting Medium
serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
3
Lowest density fraction
10%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
10,3
Sample volume (mL)
4,8
Orientation
Bottom-up
Speed (g)
200000
Duration (min)
120
Fraction volume (mL)
1
Fraction processing
None
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Density cushion
Density medium
Sucrose
Cushion volume
1,5
Density of the cushion
0.8-2.0 M
Centrifugation time
120
Centrifugation speed
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
LprG
Proteomics database
ProteomeXchange
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
40-50
EV concentration
Yes
Particle yield
Total particle count
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
20-250
|
||||||||
EV230660 | 1/1 | Pseudomonas syringae | Lz4W |
(d)(U)C DG Filtration |
Kulkarni HM | 2015 | 57% | |
Study summaryFull title
All authors
Kulkarni HM, Swamy ChV, Jagannadham MV
Journal
Data Brief
Abstract
Outer membrane vesicles (OMVs) of gram-negative bacteria are released during all growth phases and p (show more...)
EV-METRIC
57% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Pseudomonas syringae
Sample Type
Cell culture supernatant
EV-producing cells
Lz4W
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
180
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
150264
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
3
Lowest density fraction
20%
Highest density fraction
70%
Orientation
Top-down
Speed (g)
164609
Duration (min)
360
Fraction processing
Centrifugation
Pelleting: volume per fraction
4
Pelleting: speed (g)
163202
Pelleting: adjusted k-factor
7,59E
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Protein analysis
Protein Concentration Method
Bradford
Protein Yield (µg)
per million cells
Proteomics database
ProteomeXchange
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
60-80
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
60-100
|
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EV150034 | 2/2 | Homo sapiens | Blood plasma |
(d)(U)C SEC |
de Menezes-Neto A | 2015 | 57% | |
Study summaryFull title
All authors
de Menezes-Neto A, Sáez MJ, Lozano-Ramos I, Segui-Barber J, Martin-Jaular L, Ullate JM, Fernandez-Becerra C, Borrás FE, Del Portillo HA
Journal
J Extracell Vesicles
Abstract
Plasma-derived vesicles hold a promising potential for use in biomedical applications. Two major cha (show more...)
EV-METRIC
57% (89th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
Vesicles
Separation protocol
Separation protocol
(d)(U)C
SEC Protein markers
EV: CD81/ GAPDH/ CD9
non-EV: Albumin Proteomics
yes
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
GAPDH
ELISA
Detected EV-associated proteins
GAPDH
Characterization: Particle analysis
NTA
EM
EM-type
immune EM/ cryo EM
EM protein
CD5L
Image type
Close-up
|
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EV230672 | 1/4 | Vibrio cholerae | A1552 |
(d)(U)C DG Filtration |
Sjöström AE | 2015 | 56% | |
Study summaryFull title
All authors
Sjöström AE, Sandblad L, Uhlin BE, Wai SN
Journal
Sci Rep
Abstract
Many Gram-negative bacterial species release outer membrane vesicles (OMVs) that interact with the h (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Adj. k-factor
0 (pelleting)
Protein markers
EV: OmpU
non-EV: RpoS Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
A1552
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
0
Density gradient
Only used for validation of main results
Yes
Type
Discontinuous
Number of initial discontinuous layers
7
Lowest density fraction
10%
Highest density fraction
45%
Orientation
Top-down
Speed (g)
180000
Duration (min)
120
Fraction volume (mL)
0.1-0.3
Fraction processing
Centrifugation
Pelleting: volume per fraction
0.1-0.3
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
1,70E
Pelleting-wash: volume per pellet (mL)
60
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
OmpU
Not detected contaminants
RpoS
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing/ Northern blot
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Transmission-EM/ cryo-EM
Image type
Close-up, Wide-field
Report size (nm)
25-200
|
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EV230657 | 1/2 | Escherichia coli | O104:H4 |
(d)(U)C DG Filtration |
Kunsmann L | 2015 | 56% | |
Study summaryFull title
All authors
Kunsmann L, Rüter C, Bauwens A, Greune L, Glüder M, Kemper B, Fruth A, Wai SN, He X, Lloubes R, Schmidt MA, Dobrindt U, Mellmann A, Karch H, Bielaszewska M
Journal
Sci Rep
Abstract
The highly virulent Escherichia coli O104:H4 that caused the large 2011 outbreak of diarrhoea and ha (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
LB226692
Focus vesicles
Outer membrane vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Protein markers
EV: OmpA/ ShET1/ Flagellin/ Stx2a
non-EV: Pic/ SigA/ SepA Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Escherichia coli
Sample Type
Cell culture supernatant
EV-producing cells
O104:H4
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
235000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
6
Lowest density fraction
20%
Highest density fraction
45%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
2
Orientation
Bottom-up
Speed (g)
288000
Duration (min)
960
Fraction volume (mL)
1
Fraction processing
None
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Detected EV-associated proteins
OmpA/ ShET1/ Flagellin/ Stx2a
Not detected contaminants
Pic/ SigA/ SepA
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
EM
EM-type
Immuno-EM
Image type
Close-up
|
||||||||
EV230629 | 2/9 | Haemophilus influenzae | Rd KW20 |
(d)(U)C DG Filtration |
Roier S | 2015 | 56% | |
Study summaryFull title
All authors
Roier S, Blume T, Klug L, Wagner GE, Elhenawy W, Zangger K, Prassl R, Reidl J, Daum G, Feldman MF, Schild S
Journal
Int J Med Microbiol
Abstract
Outer membrane vesicles (OMVs) are spherical and bilayered particles that are naturally released fro (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Pure outer membrane vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Protein markers
EV: None
non-EV: RpoA Proteomics
yes
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)/ vaccine development
Sample
Species
Haemophilus influenzae
Sample Type
Cell culture supernatant
EV-producing cells
Rd KW20
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
240
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
144000
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Protein Yield (µg)
per mL/OD490
Western Blot
Not detected contaminants
RpoA
Proteomics database
No
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV230629 | 4/9 | Haemophilus influenzae | Hib strain Eagan |
(d)(U)C DG Filtration |
Roier S | 2015 | 56% | |
Study summaryFull title
All authors
Roier S, Blume T, Klug L, Wagner GE, Elhenawy W, Zangger K, Prassl R, Reidl J, Daum G, Feldman MF, Schild S
Journal
Int J Med Microbiol
Abstract
Outer membrane vesicles (OMVs) are spherical and bilayered particles that are naturally released fro (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Pure outer membrane vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Protein markers
EV: None
non-EV: RpoA Proteomics
yes
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)/ vaccine development
Sample
Species
Haemophilus influenzae
Sample Type
Cell culture supernatant
EV-producing cells
Hib strain Eagan
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
240
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
144000
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Protein Yield (µg)
per mL/OD490
Western Blot
Not detected contaminants
RpoA
Proteomics database
No
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV230629 | 6/9 | Haemophilus influenzae | NTHi 2019-R |
(d)(U)C DG Filtration |
Roier S | 2015 | 56% | |
Study summaryFull title
All authors
Roier S, Blume T, Klug L, Wagner GE, Elhenawy W, Zangger K, Prassl R, Reidl J, Daum G, Feldman MF, Schild S
Journal
Int J Med Microbiol
Abstract
Outer membrane vesicles (OMVs) are spherical and bilayered particles that are naturally released fro (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Pure outer membrane vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Protein markers
EV: None
non-EV: RpoA Proteomics
yes
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)/ vaccine development
Sample
Species
Haemophilus influenzae
Sample Type
Cell culture supernatant
EV-producing cells
NTHi 2019-R
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
240
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
144000
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Protein Yield (µg)
per mL/OD490
Western Blot
Not detected contaminants
RpoA
Proteomics database
No
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV140127 | 2/5 | Homo sapiens | Du145 |
(d)(U)C DG |
Webber JP | 2015 | 56% | |
Study summaryFull title
All authors
Webber JP, Spary LK, Sanders AJ, Chowdhury R, Jiang WG, Steadman R, Wymant J, Jones AT, Kynaston H, Mason MD, Tabi Z, Clayton A
Journal
Oncogene
Abstract
Activation of myofibroblast rich stroma is a rate-limiting step essential for cancer progression. Th (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: TSG101/ CD63/ TGF-beta1/ CD81/ MHC class-I/ Alix/ 5T4/ CD9
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Du145
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
No
Density gradient
Type
Not specified
Number of initial discontinuous layers
Not specified
Lowest density fraction
Not specified
Highest density fraction
Not specified
Total gradient volume, incl. sample (mL)
Not specified
Sample volume (mL)
Not spec
Orientation
Not specified
Rotor type
Not specified
Speed (g)
Not specified
Duration (min)
Not specified
Fraction volume (mL)
Not specified
Fraction processing
Not specified
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
Alix/ 5T4/ MHC class-I/ TSG101
Detected contaminants
Calnexin
ELISA
Detected EV-associated proteins
CD63/ CD81/ CD9/ TGF-beta1
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
115
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV150106 | 1/1 | Homo sapiens | HMEC-1 |
DG (d)(U)C |
van Balkom BW | 2015 | 56% | |
Study summaryFull title
All authors
van Balkom BW, Eisele AS, Pegtel DM, Bervoets S, Verhaar MC.
Journal
J Extracell Vesicles
Abstract
Exosomes are small vesicles that mediate cell-cell communication. They contain proteins, lipids and (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: GAPDH/ Flotillin1/ CD9/
non-EV: Histone H2A.X/ Lamin A / C/ ATP5A/ Tom20 Proteomics
no
EV density (g/ml)
1.07 - 1.12
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HMEC-1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
1h at 200000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Wash: time (min)
60
Wash: Rotor Type
Not specified
Wash: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
0.25M
Highest density fraction
2.0M
Sample volume (mL)
0.25
Orientation
Bottom-up
Rotor type
Not specified
Speed (g)
190000
Duration (min)
960
Fraction volume (mL)
0.4
Fraction processing
None
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
GAPDH/ Flotillin1/ CD9
Not detected contaminants
Lamin A/C/ Histone H2A.X/ ATP5A/ Tom20
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;RNAsequencing
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV150003 | 1/2 | Homo sapiens | NAY | (d)(U)C | Saari H | 2015 | 56% | |
Study summaryFull title
All authors
Saari H, Lázaro-Ibáñez E, Viitala T, Vuorimaa-Laukkanen E, Siljander P, Yliperttula M
Journal
J Control Release
Abstract
BACKGROUND: Extracellular vesicles (EVs) are naturally occurring membrane particles that mediate int (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes / microvesicles
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
52.47 (washing)
Protein markers
EV: CD81/ Alpha-tubulin/ TSG101/ CD63/ CD9
non-EV: GAPDH Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Wash: volume per pellet (ml)
1
Wash: Rotor Type
TLA55
Wash: adjusted k-factor
52.47
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD81/ CD9/ TSG101/ Alpha-tubulin
Detected contaminants
GAPDH
ELISA
Detected EV-associated proteins
Alpha-tubulin
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM/ cryo EM
Image type
Close-up
|
||||||||
EV150016 | 2/2 | Homo sapiens | Ascites |
(d)(U)C DC Filtration |
Pospichalova V | 2015 | 56% | |
Study summaryFull title
All authors
Pospichalova V, Svoboda J, Dave Z, Kotrbova A, Kaiser K, Klemova D, Ilkovics L, Hampl A, Crha I, Jandakova E, Minar L, Weinberger V, Bryja V
Journal
J Extracell Vesicles
Abstract
Flow cytometry is a powerful method, which is widely used for high-throughput quantitative and quali (show more...)
EV-METRIC
56% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Ascites
Sample origin
NAY
Focus vesicles
exosomes / microvesicles
Separation protocol
Separation protocol
(d)(U)C
DC Filtration Adj. k-factor
253.9 (pelleting)
Protein markers
EV: Alix/ HSP70/ TSG101/ CD63
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Ascites
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
190
Pelleting: rotor type
SW28
Pelleting: adjusted k-factor
253.9
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ HSP70/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
EM
EM-type
transmission EM/ immune EM
EM protein
CD63
Image type
Close-up, Wide-field
|
||||||||
EV150002 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG |
Phinney DG | 2015 | 56% | |
Study summaryFull title
All authors
Phinney DG, Di Giuseppe M, Njah J, Sala E, Shiva S, St Croix CM, Stolz DB, Watkins SC, Di YP, Leikauf GD, Kolls J, Riches DW, Deiuliis G, Kaminski N, Boregowda SV, McKenna DH, Ortiz LA
Journal
Nat Commun
Abstract
Mesenchymal stem cells (MSCs) and macrophages are fundamental components of the stem cell niche and (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: CD63/ CD9/ MFGE8
non-EV: Proteomics
no
EV density (g/ml)
1.09-1.14
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Bottom-up
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD9/ MFGE8
ELISA
Detected EV-associated proteins
MFGE8
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV150027 | 2/2 | Homo sapiens | NAY |
(d)(U)C DG Filtration |
Kharaziha P | 2015 | 56% | |
Study summaryFull title
All authors
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullén A, Yachnin J, Nilsson S, Panaretakis T
Journal
Oncotarget
Abstract
Docetaxel is a cornerstone treatment for metastatic, castration resistant prostate cancer (CRPC) whi (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV: TSG101/ CD63/ CD81/ CD82/ Alix/ Rab5/ Caveolin1/ CD9
non-EV: Cell organelle protein Proteomics
yes
EV density (g/ml)
1.12-1.19
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
120
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.2
Highest density fraction
2
Orientation
Top-down
Speed (g)
120000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ CD81/ CD9/ TSG101/ CD82/ Rab5/ Caveolin1
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
CD82/ Rab5/ Caveolin1
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV150010 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG |
Keerthikumar S | 2015 | 56% | |
Study summaryFull title
All authors
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal
Oncotarget
Abstract
Extracellular vesicles (EVs) include the exosomes (30-100 nm) that are produced through the endocyti (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes / Ectosomes
Separation protocol
Separation protocol
(d)(U)C
DG Adj. k-factor
276.6 (pelleting)
Protein markers
EV: Alix/ CD81/ TSG101/ CD63
non-EV: MMP2/ Cell organelle protein Proteomics
yes
EV density (g/ml)
1.100
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
1060
Pelleting: rotor type
SW40
Pelleting: adjusted k-factor
276.6
Density gradient
Lowest density fraction
5
Highest density fraction
40
Orientation
Top-down
Pelleting-wash: volume per pellet (mL)
1
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ CD81/ TSG101
Detected contaminants
Cell organelle protein/ MMP2
Characterization: Particle analysis
EM
EM-type
transmission EM/ atomic force EM
Image type
Wide-field
|
||||||||
EV150021 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG |
Duchez AC | 2015 | 56% | |
Study summaryFull title
All authors
Duchez AC, Boudreau LH, Bollinger J, Belleannée C, Cloutier N, Laffont B, Mendoza-Villarroel RE, Lévesque T, Rollet-Labelle E, Rousseau M, Allaeys I, Tremblay JJ, Poubelle PE, Lambeau G, Pouliot M, Provost P, Soulet D, Gelb MH, Boilard E
Journal
Proc Natl Acad Sci U S A
Abstract
Platelets are anucleated blood elements highly potent at generating extracellular vesicles (EVs) cal (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
microparticles
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: TSG101/ VDAC
non-EV: Proteomics
no
EV density (g/ml)
1.1-1.14
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
5
Highest density fraction
40
Orientation
Top-down
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
TSG101/ VDAC
ELISA
Detected EV-associated proteins
VDAC
Characterization: Particle analysis
DLS
|
||||||||
EV150103 | 7/7 | Homo sapiens | HUVEC | (d)(U)C | Dieudé M | 2015 | 55% | |
Study summaryFull title
All authors
Dieudé M, Bell C, Turgeon J, Beillevaire D, Pomerleau L, Yang B, Hamelin K, Qi S, Pallet N, Béland C, Dhahri W, Cailhier JF, Rousseau M, Duchez AC, Lévesque T, Lau A, Rondeau C, Gingras D, Muruve D, Rivard A, Cardinal H, Perreault C, Desjardins M, Boilard É, Thibault P, Hébert MJ
Journal
J Transl Med
Abstract
Autoantibodies to components of apoptotic cells, such as anti-perlecan antibodies, contribute to rej (show more...)
EV-METRIC
55% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Apoptosis
Focus vesicles
apoptotic exosome-like vesicle
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
127.9 (pelleting)
Protein markers
EV: LG3/ Fibronectin/ proteasome-alpha3/ Syntenin/ TCTP
non-EV: Tubulin/ GM130 Proteomics
yes
Show all info
Study aim
Function, Biomarker, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HUVEC
EV-harvesting Medium
Serum free medium
Cell viability (%)
NA
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
1080
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
200000
Pelleting: adjusted k-factor
127.9
Characterization: Protein analysis
Protein Concentration Method
microBCA
Protein Yield (µg)
300
Western Blot
Detected EV-associated proteins
Syntenin, Fibronectin, TCTP, proteasome-alpha3, LG3
Not detected contaminants
GM130, Tubulin
Proteomics database
Yes
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
BDCantoII Special Order Research Product
Hardware adjustment
This high sensitivity Flow cytometer (hsFCM) is equipped with a small particle option. The forward scatter (FSC) on this dedicated equipment is coupled to a photomultiplier tube (PMT) with a 488 nm solid state;100mW output blue laser (rather than the conventional 20 mW);and includes a 633nmHeNe;20mW output red laser and a 405 nm solid state diode;50mW output violet laser. The hsFCM includes a FSC-PMT and a Fourier optical transformation unit;which reduces the background noise and increases the angle of diffusion;therby enhancing the detection of small-diameter particles.
Calibration bead size
0.09,0.45,0.84,1,3.2
Report type
Median
Reported size (nm)
100-200
EV concentration
Yes
Particle yield
3.50E+07 particles/million cells
EM
EM-type
Transmission-EM/ Immune-EM
EM protein
LG3;proteasome-alpha3
Image type
Close-up, Wide-field
|
||||||||
EV150007 | 1/10 | Homo sapiens | NAY |
(d)(U)C UF qEV |
Lobb RJ | 2015 | 50% | |
Study summaryFull title
All authors
Lobb RJ, Becker M, Wen SW, Wong CS, Wiegmans AP, Leimgruber A, Möller A
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis of (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
UF qEV Protein markers
EV: TSG101/ HSP70/ Flotilin1
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Flotilin1/ HSP70/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
TRPS
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV150007 | 4/10 | Homo sapiens | NAY |
(d)(U)C Filtration UF |
Lobb RJ | 2015 | 50% | |
Study summaryFull title
All authors
Lobb RJ, Becker M, Wen SW, Wong CS, Wiegmans AP, Leimgruber A, Möller A
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis of (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Protein markers
EV: TSG101/ HSP70/ Flotilin1/ CD63
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ Flotilin1/ HSP70/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
TRPS
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV150007 | 5/10 | Homo sapiens | NAY |
(d)(U)C DG Filtration UF |
Lobb RJ | 2015 | 50% | |
Study summaryFull title
All authors
Lobb RJ, Becker M, Wen SW, Wong CS, Wiegmans AP, Leimgruber A, Möller A
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis of (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration UF Protein markers
EV: TSG101/ HSP70/ Flotilin1
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Density gradient
Lowest density fraction
5
Highest density fraction
40
Orientation
Top-down
Rotor type
50.2Ti
Speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Flotilin1/ HSP70/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
TRPS
|
||||||||
EV150007 | 7/10 | Homo sapiens | NAY |
(d)(U)C Exo-spin Filtration |
Lobb RJ | 2015 | 50% | |
Study summaryFull title
All authors
Lobb RJ, Becker M, Wen SW, Wong CS, Wiegmans AP, Leimgruber A, Möller A
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis of (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Exo-spin Filtration Protein markers
EV: TSG101/ HSP70/ Flotilin1
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
Exo-spin
Other
Name other separation method
Exo-spin
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Flotilin1/ HSP70/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
TRPS
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV150007 | 8/10 | Homo sapiens | NAY |
(d)(U)C ExoQuick Filtration |
Lobb RJ | 2015 | 50% | |
Study summaryFull title
All authors
Lobb RJ, Becker M, Wen SW, Wong CS, Wiegmans AP, Leimgruber A, Möller A
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis of (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Filtration Protein markers
EV: TSG101/ HSP70/ Flotilin1
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Flotilin1/ HSP70/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
TRPS
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV140124 | 2/6 | Homo sapiens | LNCaP | ExoQuick | Ramteke, Anand | 2015 | 50% | |
Study summaryFull title
All authors
Anand Ramteke, Harold Ting, Chapla Agarwal, Samiha Mateen, Ranganathan Somasagara, Anowar Hussain, Michael Graner, Barbara Frederick, Rajesh Agarwal, Gagan Deep
Journal
Mol Carcinog
Abstract
Hypoxic conditions in prostate cancer (PCA) are associated with poor prognosis; however, precise mec (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: CD63/ MMP-9/ MMP-2/ TGF-beta2/ TNF1alpha/ IL6/ TSG101/ Akt/ ILK1/ beta-catenin/ PKM2/ HSP90/ Annexin II/ alpha-tubulin/ / Alix/ CD81/ HSP70
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
LNCaP
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Western Blot
Detected EV-associated proteins
CD63/ HSP90/ Annexin II/ alpha-tubulin/ / MMP-9/ MMP-2/ TGF-beta2/ TNF1alpha/ IL6/ TSG101/ Akt/ ILK1/ beta-catenin/ PKM2/ HSP70/ Alix/ CD81
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
187
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 0.26E8
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV140124 | 4/6 | Homo sapiens | LNCaP | ExoQuick | Ramteke, Anand | 2015 | 50% | |
Study summaryFull title
All authors
Anand Ramteke, Harold Ting, Chapla Agarwal, Samiha Mateen, Ranganathan Somasagara, Anowar Hussain, Michael Graner, Barbara Frederick, Rajesh Agarwal, Gagan Deep
Journal
Mol Carcinog
Abstract
Hypoxic conditions in prostate cancer (PCA) are associated with poor prognosis; however, precise mec (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Hypoxia
Focus vesicles
exosome
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: CD63/ MMP-9/ MMP-2/ TGF-beta2/ TNF1alpha/ IL6/ TSG101/ Akt/ ILK1/ beta-catenin/ PKM2/ HSP90/ Annexin II/ alpha-tubulin/ / Alix/ CD81/ HSP70
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
LNCaP
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Western Blot
Detected EV-associated proteins
Alix/ Annexin II/ alpha-tubulin/ / MMP-9/ MMP-2/ TGF-beta2/ TNF1alpha/ IL6/ TSG101/ Akt/ ILK1/ beta-catenin/ PKM2/ CD63/ HSP90/ HSP70/ CD81
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
141
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 0.24E8
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230662 | 5/6 | Mycobacterium smegmatis | mc²155 |
(d)(U)C Filtration |
Kumar S | 2015 | 45% | |
Study summaryFull title
All authors
Kumar S, Mittal E, Deore S, Kumar A, Rahman A, Krishnasastry MV
Journal
Front Cell Infect Microbiol
Abstract
The mycobacterial tlyA gene product, Rv1694 (MtbTlyA), has been annotated as "hemolysin" which was r (show more...)
EV-METRIC
45% (86th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
pTlyA transformed
Focus vesicles
Membrane vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: TlyA/ DnaK
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Mycobacterium smegmatis
Sample Type
Cell culture supernatant
EV-producing cells
mc²155
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
TLA-100
Pelleting: speed (g)
150000
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
TlyA/ DnaK
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Transmission-EM/ immuno-EM
EM protein
TlyA
Image type
Close-up
|
||||||||
EV230657 | 2/2 | Escherichia coli | O104:H4 |
(d)(U)C DG Filtration |
Kunsmann L | 2015 | 45% | |
Study summaryFull title
All authors
Kunsmann L, Rüter C, Bauwens A, Greune L, Glüder M, Kemper B, Fruth A, Wai SN, He X, Lloubes R, Schmidt MA, Dobrindt U, Mellmann A, Karch H, Bielaszewska M
Journal
Sci Rep
Abstract
The highly virulent Escherichia coli O104:H4 that caused the large 2011 outbreak of diarrhoea and ha (show more...)
EV-METRIC
45% (86th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
C227-11Φcu
Focus vesicles
Outer membrane vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Protein markers
EV: OmpA/ ShET1/ Flagellin
non-EV: Pic/ SigA/ SepA Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Escherichia coli
Sample Type
Cell culture supernatant
EV-producing cells
O104:H4
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
235000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
6
Lowest density fraction
20%
Highest density fraction
45%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
2
Orientation
Bottom-up
Speed (g)
288000
Duration (min)
960
Fraction volume (mL)
1
Fraction processing
None
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Detected EV-associated proteins
OmpA/ ShET1/ Flagellin
Not detected contaminants
Pic/ SigA/ SepA
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
None
|
||||||||
EV230510 | 2/4 | Mus musculus | RAW264.7 |
(d)(U)C DG |
Athman JJ | 2015 | 44% | |
Study summaryFull title
All authors
Athman JJ, Wang Y, McDonald DJ, Boom WH, Harding CV, Wearsch PA
Journal
J Immunol
Abstract
Mycobacterium tuberculosis is an intracellular pathogen that infects lung macrophages and releases m (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Live mycobacterium tubercolusis
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: CD9/ CD63/ LpqH/ LAM/ LM/ MHCII/ Mtb
non-EV: None Proteomics
no
EV density (g/ml)
1.11-1.17
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
RAW264.7
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: time (min)
70
Wash: Rotor Type
TLA-100.3
Wash: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
0.2 M
Highest density fraction
1.8 M
Total gradient volume, incl. sample (mL)
11.7
Sample volume (mL)
0.2
Orientation
Top-down
Speed (g)
110000
Duration (min)
1140
Fraction volume (mL)
1
Fraction processing
Precipitation of all proteins/vesicles (e.g. through acidification, Amphipol,...)
Other
Name other separation method
Density gradient
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
CD9/ CD63/ LpqH/ LAM/ LM
Other 1
Immunofluorescence
Detected EV-associated proteins
CD9/ CD63/ Mtb
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Modus
Reported size (nm)
95-105
EM
EM-type
Transmission-EM/ Immuno-EM
EM protein
CD9/ MHCII/ Mtb
Image type
Close-up
|
||||||||
EV210504 | 1/5 | Macaca mulatta | Brain tissue |
(d)(U)C DG |
Yelamanchili SV | 2015 | 44% | |
Study summaryFull title
All authors
Yelamanchili SV, Lamberty BG, Rennard DA, Morsey BM, Hochfelder CG, Meays BM, Levy E, Fox HS
Journal
PLoS Pathog
Abstract
Recent studies have found that extracellular vesicles (EVs) play an important role in normal and dis (show more...)
EV-METRIC
44% (51st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Brain tissue
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: TSG101/ CD63/ CD81/ SNAP 25/ HSP70/ Synaptophysin/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Macaca mulatta
Sample Type
Brain tissue
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
37
Wash: time (min)
60
Wash: speed (g)
100000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
6
Lowest density fraction
0.25M
Highest density fraction
2.0M
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
2
Speed (g)
200000
Duration (min)
960
Fraction volume (mL)
6
Fraction processing
Centrifugation
Pelleting: duration (min)
60
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD9/ CD63/ TSG101/ HSP70/ CD81
Not detected EV-associated proteins
Synaptophysin/ SNAP 25
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR/ RNA sequencing
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV210395 | 1/1 | Homo sapiens | Bone-marrow derived mesenchymal stem cells | dUC | Shabbir A | 2015 | 44% | |
Study summaryFull title
All authors
Shabbir A, Cox A, Rodriguez-Menocal L, Salgado M, Van Badiavas E
Journal
Stem Cells Dev
Abstract
Although chronic wounds are common and continue to be a major cause of morbidity and mortality, trea (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
dUC
Protein markers
EV: Flotillin1/ CD9/ CD63/ Beta-actin/ GAPDH/ phosphoStat3 (Tyr750)/ total Stat3/ phospho AKT (Ser473)/ total AKT/ phospho ERK 1/2 (Thr202/204)/ total ERK 1/2/ TSG101/ HSP70/ Alix/ CD81
non-EV: GM130 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Bone-marrow derived mesenchymal stem cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
3h at 100,000g/ Other preparation
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Wash: time (min)
70
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
100000
Other
Name other separation method
dUC
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
Flotillin1/ CD9/ CD63/ Beta-actin/ GAPDH/ phosphoStat3 (Tyr750)/ total Stat3/ phospho AKT (Ser473)/ total AKT/ phospho ERK 1/2 (Thr202/204)/ total ERK
Not detected contaminants
GM130
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
30-100
|
||||||||
EV210169 | 1/1 | Homo sapiens | Blood plasma |
(d)(U)C IAF Filtration DG |
Guescini, Michele | 2015 | 44% | |
Study summaryFull title
Muscle Releases Alpha-Sarcoglycan Positive Extracellular Vesicles Carrying miRNAs in the Bloodstream
All authors
Michele Guescini, Barbara Canonico, Francesco Lucertini, Serena Maggio, Giosué Annibalini, Elena Barbieri, Francesca Luchetti, Stefano Papa, Vilberto Stocchi
Journal
PLoS One
Abstract
In the past few years, skeletal muscle has emerged as an important secretory organ producing soluble (show more...)
EV-METRIC
44% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Immunoaffinity capture (non-commercial) Filtration DG Protein markers
EV: CD81/ TSG101/ Alpha-sarcoglycan
non-EV: None Proteomics
no
EV density (g/ml)
1.11
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Not specified
Pelleting: speed (g)
110000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5
Highest density fraction
40
Total gradient volume, incl. sample (mL)
12.5
Sample volume (mL)
0.5
Orientation
Top-down
Rotor type
Not specified
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
1
Pelleting: duration (min)
60
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Immunoaffinity capture
Selected surface protein(s)
Alpha-sarcoglycan
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Detected EV-associated proteins
Alpha-sarcoglycan/ TSG101
Flow cytometry
Type of Flow cytometry
Not specified
Calibration bead size
1
Detected EV-associated proteins
CD81/ Alpha-sarcoglycan
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
|
||||||||
EV210117 | 1/3 | Homo sapiens | Kelly | (d)(U)C | Helge Haug, Bjørn | 2015 | 44% | |
Study summaryFull title
Exosome-like Extracellular Vesicles from MYCN-amplified Neuroblastoma Cells Contain Oncogenic miRNAs
All authors
Bjørn Helge Haug, Øyvind H Hald, Peter Utnes, Sarah A Roth, Cecilie Løkke, Trond Flægstad, Christer Einvik
Journal
Anticancer Res
Abstract
Background: In recent years, evidence has accumulated indicating that both normal and cancer cells c (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ CD63/ CD9
non-EV: actin/ N-myc/ GRP78 Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Kelly
EV-harvesting Medium
EV-depleted medium;Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Preparation of EDS
overnight (16h) at >=100,000g + filtration 0.2 µm
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Not specified
Pelleting: speed (g)
110 000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Western Blot
Detected EV-associated proteins
CD9/ CD63/ TSG101
Not detected contaminants
actin/ N-myc/ GRP78
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;Capillary electrophoresis (e.g. Bioanalyzer)
Database
No
Proteinase treatment
No
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
RNase A
RNAse concentration
5 U/ml
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
50-350
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
50-350
|
||||||||
EV210090 | 1/6 | Simiiformes | CSF | (d)(U)C | Yuyama, Kohei | 2015 | 44% | |
Study summaryFull title
All authors
Kohei Yuyama, Hui Sun, Seigo Usuki, Shota Sakai, Hisatoshi Hanamatsu, Tetsuo Mioka, Nobuyuki Kimura, Megumi Okada, Hidetoshi Tahara, Jun-ichi Furukawa, Naoki Fujitani, Yasuro Shinohara, Yasuyuki Igarashi
Journal
FEBS Lett
Abstract
Elevated amyloid-β peptide (Aβ) in brain contributes to Alzheimer's disease (AD) pathogenesis. We (show more...)
EV-METRIC
44% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
CSF
Sample origin
Cynomolgus monkey
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Flotillin1/ GM-1/ Amyloid-β/ Alix
non-EV: Tranferrin receptor/ GM130 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Simiiformes
Sample Type
CSF
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
Flotillin1/ GM-1/ Amyloid-β/ Alix
Not detected contaminants
Tranferrin receptor/ GM130
ELISA
Detected EV-associated proteins
Amyloid beta
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Not Reported
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV150108 | 3/4 | Homo sapiens | MCF-7 | (d)(U)C | Tosar JP | 2015 | 44% | |
Study summaryFull title
All authors
Tosar JP, Gámbaro F, Sanguinetti J, Bonilla B, Witwer KW, Cayota A.
Journal
Nucleic Acids Res
Abstract
Intercellular communication can be mediated by extracellular small regulatory RNAs (sRNAs). Circulat (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MCF-7
EV-harvesting Medium
Serum free medium
Cell viability (%)
95
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
Not specified
Pelleting: speed (g)
16000
Wash: time (min)
30
Wash: Rotor Type
Not specified
Wash: speed (g)
16000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Not detected EV-associated proteins
CD9/ CD63/ TSG101
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing;ddPCR
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Not Reported
NTA
Report type
Not Reported
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV160016 | 1/3 | Homo sapiens | DKO-1 |
(d)(U)C UF Filtration |
Cha DJ | 2015 | 44% | |
Study summaryFull title
All authors
Cha DJ, Franklin JL, Dou Y, Liu Q, Higginbotham JN, Demory Beckler M, Weaver AM, Vickers K, Prasad N, Levy S, Zhang B, Coffey RJ, Patton JG.
Journal
Elife
Abstract
Mutant KRAS colorectal cancer (CRC) cells release protein-laden exosomes that can alter the tumor mi (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
UF Filtration Protein markers
EV: "Flotillin1/ TSG101/ HSP70/ KRAS/ EGFR/ RAP1/ SRC/ LYN/ ITGB1/ ITGA2/ ITGAV/ ITGA6/ ITGB4/ EPHA2/ EPS8/ CTTN"
non-EV: VDAC Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
DKO-1
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Not specified
Pelleting: speed (g)
150000
Wash: time (min)
180
Wash: Rotor Type
Not specified
Wash: speed (g)
150000
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Not specified
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Detected EV-associated proteins
"Flotillin1/ TSG101/ HSP70/ KRAS/ EGFR/ RAP1/ SRC/ LYN/ ITGB1/ ITGA2/ ITGAV/ ITGA6/ ITGB4/ EPHA2/ EPS8/ CTTN"
Not detected contaminants
VDAC
Proteomics database
No
Characterization: RNA analysis
RNA analysis
Type
"(RT)(q)PCR;RNA sequencing"
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
56.3
|
||||||||
EV160016 | 3/3 | Homo sapiens | DKs-8 |
(d)(U)C UF Filtration |
Cha DJ | 2015 | 44% | |
Study summaryFull title
All authors
Cha DJ, Franklin JL, Dou Y, Liu Q, Higginbotham JN, Demory Beckler M, Weaver AM, Vickers K, Prasad N, Levy S, Zhang B, Coffey RJ, Patton JG.
Journal
Elife
Abstract
Mutant KRAS colorectal cancer (CRC) cells release protein-laden exosomes that can alter the tumor mi (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
UF Filtration Protein markers
EV: "Flotillin1/ TSG101/ HSP70/ EGFR/ RAP1/ SRC/ ITGB1/ ITGA2/ ITGAV/ CTNNA"
non-EV: VDAC Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
DKs-8
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Not specified
Pelleting: speed (g)
150000
Wash: time (min)
180
Wash: Rotor Type
Not specified
Wash: speed (g)
150000
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Not specified
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Detected EV-associated proteins
"Flotillin1/ TSG101/ HSP70/ EGFR/ RAP1/ SRC/ ITGB1/ ITGA2/ ITGAV/ CTNNA"
Not detected contaminants
VDAC
Proteomics database
No
Characterization: RNA analysis
RNA analysis
Type
"(RT)(q)PCR;RNA sequencing"
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
59.2
|
||||||||
EV150104 | 1/4 | Homo sapiens | Urine |
DC (d)(U)C |
Pocsfalvi G | 2015 | 44% | |
Study summaryFull title
All authors
Pocsfalvi G, Raj DA, Fiume I, Vilasi A, Trepiccione F, Capasso G.
Journal
Proteomics Clin Appl
Abstract
PURPOSE:
Recent findings indicate that urinary extracellular vesicles (EVs) might reflect the pathop (show more...)
EV-METRIC
44% (81st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DC
(d)(U)C Protein markers
EV: TSG101/ Alix/ AQP2/ PKD22/ NHE3/ CD9/ PKD11
non-EV: / Uromodulin/ Albumin Proteomics
yes
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
Not specified
Pelleting: speed (g)
200000
Density cushion
Density medium
Sucrose
|