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You searched for: EV150034 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV150034 | 2/2 | Homo sapiens | Blood plasma |
(d)(U)C SEC |
de Menezes-Neto A | 2015 | 57% | |
Study summaryFull title
All authors
de Menezes-Neto A, Sáez MJ, Lozano-Ramos I, Segui-Barber J, Martin-Jaular L, Ullate JM, Fernandez-Becerra C, Borrás FE, Del Portillo HA
Journal
J Extracell Vesicles
Abstract
Plasma-derived vesicles hold a promising potential for use in biomedical applications. Two major cha (show more...)
EV-METRIC
57% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
Vesicles
Separation protocol
Separation protocol
(d)(U)C
SEC Protein markers
EV: CD81/ GAPDH/ CD9
non-EV: Albumin Proteomics
yes
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
GAPDH
ELISA
Detected EV-associated proteins
GAPDH
Characterization: Particle analysis
NTA
EM
EM-type
immune EM/ cryo EM
EM protein
CD5L
Image type
Close-up
|
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EV150034 | 1/2 | Homo sapiens | Blood plasma |
(d)(U)C Exospin |
de Menezes-Neto A | 2015 | 17% | |
Study summaryFull title
All authors
de Menezes-Neto A, Sáez MJ, Lozano-Ramos I, Segui-Barber J, Martin-Jaular L, Ullate JM, Fernandez-Becerra C, Borrás FE, Del Portillo HA
Journal
J Extracell Vesicles
Abstract
Plasma-derived vesicles hold a promising potential for use in biomedical applications. Two major cha (show more...)
EV-METRIC
17% (45th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
Vesicles
Separation protocol
Separation protocol
(d)(U)C
Exospin Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Pelleting performed
No
Commercial kit
Exospin
Other
Name other separation method
Exospin
Characterization: Protein analysis
Characterization: Particle analysis
NTA
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1 - 2 of 2 |
EV-TRACK ID | EV150034 | |
---|---|---|
species | Homo sapiens | |
sample type | Blood plasma | |
condition | NAY | |
separation protocol | (d)(U)C SEC | (d)(U)C Exospin |
Exp. nr. | 2 | 1 |
EV-METRIC % | 57 | 17 |