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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV230660	1/1	Pseudomonas syringae	Lz4W	(d)(U)C DG Filtration	Kulkarni HM	2015	57%
Study summary Full title The proteome of the outer membrane vesicles of an Antarctic bacterium Pseudomonas syringae Lz4W. All authors Kulkarni HM, Swamy ChV, Jagannadham MV Journal Data Brief Abstract Outer membrane vesicles (OMVs) of gram-negative bacteria are released during all growth phases and p (show more...)Outer membrane vesicles (OMVs) of gram-negative bacteria are released during all growth phases and play an important role in bacterial physiology. They consist of lipids, proteins, lipopolysaccharides and other molecules. The OMVs of the Antarctic bacterium Pseudomonas syringae Lz 4W were isolated and identified their proteins. The mass spectral data set deposited with PRIDE, accession number PXD 000221 is presented in this report. The proteins identified from the OMVs of P. syringae Lz4W, data of this study were published in the Journal of proteome research [1]. (hide) EV-METRIC 57% (92nd percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Cell culture supernatant Sample origin Control condition Focus vesicles Outer membrane vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (Differential) (ultra)centrifugation Density gradient Filtration Protein markers EV: None non-EV: None Proteomics yes Show all info Study aim Identification of content (omics approaches) Sample Species Pseudomonas syringae Sample Type Cell culture supernatant EV-producing cells Lz4W EV-harvesting Medium Serum free medium Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed Yes Pelleting: time(min) 180 Pelleting: rotor type Type 45 Ti Pelleting: speed (g) 150264 Density gradient Type Discontinuous Number of initial discontinuous layers 3 Lowest density fraction 20% Highest density fraction 70% Orientation Top-down Speed (g) 164609 Duration (min) 360 Fraction processing Centrifugation Pelleting: volume per fraction 4 Pelleting: speed (g) 163202 Pelleting: adjusted k-factor 7,59E Filtration steps 0.45µm > x > 0.22µm, Characterization: Protein analysis Protein Concentration Method Bradford Protein Yield (µg) per million cells Proteomics database ProteomeXchange Characterization: Lipid analysis Yes Characterization: Particle analysis DLS Report type Size range/distribution Reported size (nm) 60-80 EM EM-type Transmission-EM Image type Wide-field Report size (nm) 60-100

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EV-TRACK ID	EV230660
species	Pseudomonas syringae
sample type	Cell culture
cell type	Lz4W
condition	Control condition
separation protocol	dUC/ Density gradient/ Filtration
Exp. nr.	1
EV-METRIC %	57