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You searched for: EV210117 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210117 | 1/3 | Homo sapiens | Kelly | (d)(U)C | Helge Haug, Bjørn | 2015 | 44% | |
Study summaryFull title
Exosome-like Extracellular Vesicles from MYCN-amplified Neuroblastoma Cells Contain Oncogenic miRNAs
All authors
Bjørn Helge Haug, Øyvind H Hald, Peter Utnes, Sarah A Roth, Cecilie Løkke, Trond Flægstad, Christer Einvik
Journal
Anticancer Res
Abstract
Background: In recent years, evidence has accumulated indicating that both normal and cancer cells c (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ CD63/ CD9
non-EV: actin/ N-myc/ GRP78 Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Kelly
EV-harvesting Medium
EV-depleted medium;Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Preparation of EDS
overnight (16h) at >=100,000g + filtration 0.2 µm
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Not specified
Pelleting: speed (g)
110 000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Western Blot
Detected EV-associated proteins
CD9/ CD63/ TSG101
Not detected contaminants
actin/ N-myc/ GRP78
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;Capillary electrophoresis (e.g. Bioanalyzer)
Database
No
Proteinase treatment
No
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
RNase A
RNAse concentration
5 U/ml
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
50-350
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
50-350
|
||||||||
EV210117 | 3/3 | Homo sapiens | SK-N-BE(2)-C | (d)(U)C | Helge Haug, Bjørn | 2015 | 33% | |
Study summaryFull title
Exosome-like Extracellular Vesicles from MYCN-amplified Neuroblastoma Cells Contain Oncogenic miRNAs
All authors
Bjørn Helge Haug, Øyvind H Hald, Peter Utnes, Sarah A Roth, Cecilie Løkke, Trond Flægstad, Christer Einvik
Journal
Anticancer Res
Abstract
Background: In recent years, evidence has accumulated indicating that both normal and cancer cells c (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ CD63/ CD9
non-EV: actin/ N-myc/ GRP78 Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
SK-N-BE(2)-C
EV-harvesting Medium
EV-depleted medium;Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Preparation of EDS
overnight (16h) at >=100,000g + filtration 0.2 µm
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Not specified
Pelleting: speed (g)
110 000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Western Blot
Detected EV-associated proteins
CD9/ CD63/ TSG101
Not detected contaminants
actin/ N-myc/ GRP78
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;Capillary electrophoresis (e.g. Bioanalyzer)
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210117 | 2/3 | Homo sapiens | Kelly |
(d)(U)C ExoQuick |
Helge Haug, Bjørn | 2015 | 0% | |
Study summaryFull title
Exosome-like Extracellular Vesicles from MYCN-amplified Neuroblastoma Cells Contain Oncogenic miRNAs
All authors
Bjørn Helge Haug, Øyvind H Hald, Peter Utnes, Sarah A Roth, Cecilie Løkke, Trond Flægstad, Christer Einvik
Journal
Anticancer Res
Abstract
Background: In recent years, evidence has accumulated indicating that both normal and cancer cells c (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Commercial method Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Kelly
EV-harvesting Medium
EV-depleted medium;Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Preparation of EDS
overnight (16h) at >=100,000g + filtration 0.2 µm
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
No
Commercial kit
ExoQuick
Characterization: Protein analysis
None
Protein Concentration Method
Lowry-based assay
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
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1 - 3 of 3 |
EV-TRACK ID | EV210117 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Cell culture | ||
cell type | Kelly | SK-N-BE(2)-C | Kelly |
condition | Control condition | Control condition | Control condition |
separation protocol | (d)(U)C | (d)(U)C | (d)(U)C ExoQuick |
Exp. nr. | 1 | 3 | 2 |
EV-METRIC % | 44 | 33 | 0 |