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You searched for: EV150108 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV150108 | 1/4 | Homo sapiens | MCF-7 |
(d)(U)C Filtration |
Tosar JP | 2015 | 67% | |
Study summaryFull title
All authors
Tosar JP, Gámbaro F, Sanguinetti J, Bonilla B, Witwer KW, Cayota A.
Journal
Nucleic Acids Res
Abstract
Intercellular communication can be mediated by extracellular small regulatory RNAs (sRNAs). Circulat (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: TSG101/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MCF-7
EV-harvesting Medium
Serum free medium
Cell viability (%)
95
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
150
Pelleting: rotor type
SW 40 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
7
Wash: time (min)
150
Wash: Rotor Type
SW 40 Ti
Wash: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD9/ CD63/ TSG101
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing;ddPCR
Database
Yes
Proteinase treatment
Yes
Moment of Proteinase treatment
After
Proteinase type
Proteinase K
Proteinase concentration
64
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
RNase A
RNAse concentration
4U/mL
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Not Reported
NTA
Report type
Not Reported
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV150108 | 3/4 | Homo sapiens | MCF-7 | (d)(U)C | Tosar JP | 2015 | 44% | |
Study summaryFull title
All authors
Tosar JP, Gámbaro F, Sanguinetti J, Bonilla B, Witwer KW, Cayota A.
Journal
Nucleic Acids Res
Abstract
Intercellular communication can be mediated by extracellular small regulatory RNAs (sRNAs). Circulat (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MCF-7
EV-harvesting Medium
Serum free medium
Cell viability (%)
95
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
Not specified
Pelleting: speed (g)
16000
Wash: time (min)
30
Wash: Rotor Type
Not specified
Wash: speed (g)
16000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Not detected EV-associated proteins
CD9/ CD63/ TSG101
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing;ddPCR
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Not Reported
NTA
Report type
Not Reported
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV150108 | 2/4 | Homo sapiens | MCF-10A |
(d)(U)C Filtration |
Tosar JP | 2015 | 14% | |
Study summaryFull title
All authors
Tosar JP, Gámbaro F, Sanguinetti J, Bonilla B, Witwer KW, Cayota A.
Journal
Nucleic Acids Res
Abstract
Intercellular communication can be mediated by extracellular small regulatory RNAs (sRNAs). Circulat (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MCF-10A
EV-harvesting Medium
Serum free medium
Cell viability (%)
95
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
150
Pelleting: rotor type
SW 40 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
7
Wash: time (min)
150
Wash: Rotor Type
SW 40 Ti
Wash: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
None
Protein Concentration Method
BCA
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing;ddPCR
Database
Yes
Proteinase treatment
Yes
Moment of Proteinase treatment
After
Proteinase type
Proteinase K
Proteinase concentration
64
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
RNase A
RNAse concentration
4U/mL
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV150108 | 4/4 | Homo sapiens | MCF-10A | (d)(U)C | Tosar JP | 2015 | 0% | |
Study summaryFull title
All authors
Tosar JP, Gámbaro F, Sanguinetti J, Bonilla B, Witwer KW, Cayota A.
Journal
Nucleic Acids Res
Abstract
Intercellular communication can be mediated by extracellular small regulatory RNAs (sRNAs). Circulat (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MCF-10A
EV-harvesting Medium
Serum free medium
Cell viability (%)
95
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
Not specified
Pelleting: speed (g)
16000
Wash: time (min)
30
Wash: Rotor Type
Not specified
Wash: speed (g)
16000
Characterization: Protein analysis
None
Protein Concentration Method
BCA
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing;ddPCR
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
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1 - 4 of 4 |
EV-TRACK ID | EV150108 | |||
---|---|---|---|---|
species | Homo sapiens | |||
sample type | Cell culture | |||
cell type | MCF-7 | MCF-7 | MCF-10A | MCF-10A |
condition | Control condition | Control condition | Control condition | Control condition |
separation protocol | (d)(U)C Filtration | (d)(U)C | (d)(U)C Filtration | (d)(U)C |
Exp. nr. | 1 | 3 | 2 | 4 |
EV-METRIC % | 67 | 44 | 14 | 0 |