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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV210395	1/1	Homo sapiens	Bone-marrow derived mesenchymal stem cells	dUC	Shabbir A	2015	44%
Study summary Full title Mesenchymal Stem Cell Exosomes Induce Proliferation and Migration of Normal and Chronic Wound Fibroblasts, and Enhance Angiogenesis In Vitro. All authors Shabbir A, Cox A, Rodriguez-Menocal L, Salgado M, Van Badiavas E Journal Stem Cells Dev Abstract Although chronic wounds are common and continue to be a major cause of morbidity and mortality, trea (show more...)Although chronic wounds are common and continue to be a major cause of morbidity and mortality, treatments for these conditions are lacking and often ineffective. A large body of evidence exists demonstrating the therapeutic potential of mesenchymal stem cells (MSCs) for repair and regeneration of damaged tissue, including acceleration of cutaneous wound healing. However, the exact mechanisms of wound healing mediated by MSCs are unclear. In this study, we examined the role of MSC exosomes in wound healing. We found that MSC exosomes ranged from 30 to 100-nm in diameter and internalization of MSC exosomes resulted in a dose-dependent enhancement of proliferation and migration of fibroblasts derived from normal donors and chronic wound patients. Uptake of MSC exosomes by human umbilical vein endothelial cells also resulted in dose-dependent increases of tube formation by endothelial cells. MSC exosomes were found to activate several signaling pathways important in wound healing (Akt, ERK, and STAT3) and induce the expression of a number of growth factors [hepatocyte growth factor (HGF), insulin-like growth factor-1 (IGF1), nerve growth factor (NGF), and stromal-derived growth factor-1 (SDF1)]. These findings represent a promising opportunity to gain insight into how MSCs may mediate wound healing. (hide) EV-METRIC 44% (84th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Cell culture supernatant Sample origin Control condition Focus vesicles exosome Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture dUC Protein markers EV: Flotillin1/ CD9/ CD63/ Beta-actin/ GAPDH/ phosphoStat3 (Tyr750)/ total Stat3/ phospho AKT (Ser473)/ total AKT/ phospho ERK 1/2 (Thr202/204)/ total ERK 1/2/ TSG101/ HSP70/ Alix/ CD81 non-EV: GM130 Proteomics no Show all info Study aim Function Sample Species Homo sapiens Sample Type Cell culture supernatant EV-producing cells Bone-marrow derived mesenchymal stem cells EV-harvesting Medium EV-depleted medium Preparation of EDS 3h at 100,000g/ Other preparation Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed Yes Pelleting: rotor type Type 70 Ti Pelleting: speed (g) 100000 Wash: time (min) 70 Wash: Rotor Type Type 70 Ti Wash: speed (g) 100000 Other Name other separation method dUC Characterization: Protein analysis Protein Concentration Method BCA Western Blot Antibody details provided? No Antibody dilution provided? Yes Lysis buffer provided? Yes Detected EV-associated proteins Flotillin1/ CD9/ CD63/ Beta-actin/ GAPDH/ phosphoStat3 (Tyr750)/ total Stat3/ phospho AKT (Ser473)/ total AKT/ phospho ERK 1/2 (Thr202/204)/ total ERK Not detected contaminants GM130 Characterization: Lipid analysis No EM EM-type Transmission-EM Image type Close-up Report size (nm) 30-100

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EV-TRACK ID	EV210395
species	Homo sapiens
sample type	Cell culture
cell type	Bone-marrow derived mesenchymal stem cells
condition	Control condition
separation protocol	dUC
Exp. nr.	1
EV-METRIC %	44