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You searched for: EV140127 (EV-TRACK ID)
Showing 1 - 9 of 9
Showing 1 - 9 of 9
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140127 | 2/5 | Homo sapiens | Du145 |
(d)(U)C DG |
Webber JP | 2015 | 56% | |
Study summaryFull title
All authors
Webber JP, Spary LK, Sanders AJ, Chowdhury R, Jiang WG, Steadman R, Wymant J, Jones AT, Kynaston H, Mason MD, Tabi Z, Clayton A
Journal
Oncogene
Abstract
Activation of myofibroblast rich stroma is a rate-limiting step essential for cancer progression. Th (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: TSG101/ CD63/ TGF-beta1/ CD81/ MHC class-I/ Alix/ 5T4/ CD9
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Du145
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
No
Density gradient
Type
Not specified
Number of initial discontinuous layers
Not specified
Lowest density fraction
Not specified
Highest density fraction
Not specified
Total gradient volume, incl. sample (mL)
Not specified
Sample volume (mL)
Not spec
Orientation
Not specified
Rotor type
Not specified
Speed (g)
Not specified
Duration (min)
Not specified
Fraction volume (mL)
Not specified
Fraction processing
Not specified
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
Alix/ 5T4/ MHC class-I/ TSG101
Detected contaminants
Calnexin
ELISA
Detected EV-associated proteins
CD63/ CD81/ CD9/ TGF-beta1
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
115
EM
EM-type
Transmission-EM
Image type
Close-up
|
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EV140127 | 1/4 | Homo sapiens | Blood plasma |
Filtration dUC |
Vrijenhoek JE | 2015 | 14% | |
Study summaryFull title
All authors
Vrijenhoek JE, Pasterkamp G, Moll FL, de Borst GJ, Bots ML, Catanzariti L, van de Weg SM, de Kleijn DP, Visseren FL, Ruijter HM,
Journal
Eur J Prev Cardiol
Abstract
In patients with established cardiovascular disease, high levels of the extracellular vesicle (EV)-d (show more...)
EV-METRIC
14% (38th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Coronary artery disease
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
dUC Protein markers
EV: Cystatin c/ CD14/ alpha2-antiplasmin
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.45µm > x > 0.22µm,
Commercial kit
ExoQuick
Other
Name other separation method
dUC
Protein Concentration Method
BCA
Detected EV-associated proteins
Cystatin c/ CD14/ alpha2-antiplasmin
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV140127 | 2/4 | Homo sapiens | Blood plasma |
Filtration dUC |
Vrijenhoek JE | 2015 | 14% | |
Study summaryFull title
All authors
Vrijenhoek JE, Pasterkamp G, Moll FL, de Borst GJ, Bots ML, Catanzariti L, van de Weg SM, de Kleijn DP, Visseren FL, Ruijter HM,
Journal
Eur J Prev Cardiol
Abstract
In patients with established cardiovascular disease, high levels of the extracellular vesicle (EV)-d (show more...)
EV-METRIC
14% (38th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Cerebrovascular disease
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
dUC Protein markers
EV: Cystatin c/ CD14/ alpha2-antiplasmin
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.45µm > x > 0.22µm,
Commercial kit
ExoQuick
Other
Name other separation method
dUC
Protein Concentration Method
BCA
Detected EV-associated proteins
Cystatin c/ CD14/ alpha2-antiplasmin
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV140127 | 3/4 | Homo sapiens | Blood plasma |
Filtration dUC |
Vrijenhoek JE | 2015 | 14% | |
Study summaryFull title
All authors
Vrijenhoek JE, Pasterkamp G, Moll FL, de Borst GJ, Bots ML, Catanzariti L, van de Weg SM, de Kleijn DP, Visseren FL, Ruijter HM,
Journal
Eur J Prev Cardiol
Abstract
In patients with established cardiovascular disease, high levels of the extracellular vesicle (EV)-d (show more...)
EV-METRIC
14% (38th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Peripheral arterial disease
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
dUC Protein markers
EV: Cystatin c/ CD14/ alpha2-antiplasmin
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.45µm > x > 0.22µm,
Commercial kit
ExoQuick
Other
Name other separation method
dUC
Protein Concentration Method
BCA
Detected EV-associated proteins
Cystatin c/ CD14/ alpha2-antiplasmin
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV140127 | 4/4 | Homo sapiens | Blood plasma |
Filtration dUC |
Vrijenhoek JE | 2015 | 14% | |
Study summaryFull title
All authors
Vrijenhoek JE, Pasterkamp G, Moll FL, de Borst GJ, Bots ML, Catanzariti L, van de Weg SM, de Kleijn DP, Visseren FL, Ruijter HM,
Journal
Eur J Prev Cardiol
Abstract
In patients with established cardiovascular disease, high levels of the extracellular vesicle (EV)-d (show more...)
EV-METRIC
14% (38th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Abdominal aortic aneurysm
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
dUC Protein markers
EV: Cystatin c/ CD14/ alpha2-antiplasmin
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.45µm > x > 0.22µm,
Commercial kit
ExoQuick
Other
Name other separation method
dUC
Protein Concentration Method
BCA
Detected EV-associated proteins
Cystatin c/ CD14/ alpha2-antiplasmin
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV140127 | 1/5 | Homo sapiens | Du145 |
(d)(U)C DC |
Webber JP | 2015 | 11% | |
Study summaryFull title
All authors
Webber JP, Spary LK, Sanders AJ, Chowdhury R, Jiang WG, Steadman R, Wymant J, Jones AT, Kynaston H, Mason MD, Tabi Z, Clayton A
Journal
Oncogene
Abstract
Activation of myofibroblast rich stroma is a rate-limiting step essential for cancer progression. Th (show more...)
EV-METRIC
11% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: TSG101/ Alix/ TGF-beta1/ Lamp2/ MHC class-I/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Du145
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Density cushion
Density medium
Sucrose
Sample volume
Not specified
Cushion volume
Not specified
Density of the cushion
Not specified
Centrifugation time
120
Centrifugation speed
100000
Other
Name other separation method
Density cushion
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
TGF-beta1/ Lamp2/ TSG101/ Alix
ELISA
Detected EV-associated proteins
MHC class-I/ CD9
Characterization: Lipid analysis
No
|
||||||||
EV140127 | 3/5 | Homo sapiens | Du145 | (d)(U)C | Webber JP | 2015 | 0% | |
Study summaryFull title
All authors
Webber JP, Spary LK, Sanders AJ, Chowdhury R, Jiang WG, Steadman R, Wymant J, Jones AT, Kynaston H, Mason MD, Tabi Z, Clayton A
Journal
Oncogene
Abstract
Activation of myofibroblast rich stroma is a rate-limiting step essential for cancer progression. Th (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Rab27a K.D.
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: MHC class-I/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Du145
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
ELISA
Detected EV-associated proteins
CD9/ MHC class-I
Characterization: Lipid analysis
No
|
||||||||
EV140127 | 4/5 | Homo sapiens | Du145 | (d)(U)C | Webber JP | 2015 | 0% | |
Study summaryFull title
All authors
Webber JP, Spary LK, Sanders AJ, Chowdhury R, Jiang WG, Steadman R, Wymant J, Jones AT, Kynaston H, Mason MD, Tabi Z, Clayton A
Journal
Oncogene
Abstract
Activation of myofibroblast rich stroma is a rate-limiting step essential for cancer progression. Th (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD9/ MHC class-I
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Du145
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
ELISA
Detected EV-associated proteins
CD9/ MHC class-I
Characterization: Lipid analysis
No
|
||||||||
EV140127 | 5/5 | Homo sapiens | LNCAP |
(d)(U)C DC |
Webber JP | 2015 | 0% | |
Study summaryFull title
All authors
Webber JP, Spary LK, Sanders AJ, Chowdhury R, Jiang WG, Steadman R, Wymant J, Jones AT, Kynaston H, Mason MD, Tabi Z, Clayton A
Journal
Oncogene
Abstract
Activation of myofibroblast rich stroma is a rate-limiting step essential for cancer progression. Th (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: TGF-beta1
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
LNCAP
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
No
Density cushion
Density medium
Sucrose
Sample volume
Not specified
Cushion volume
Not specified
Density of the cushion
Not specified
Centrifugation time
120
Centrifugation speed
100000
Other
Name other separation method
Density cushion
Characterization: Protein analysis
Protein Concentration Method
BCA
ELISA
Detected EV-associated proteins
TGF-beta1
Characterization: Lipid analysis
No
|
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