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You searched for: 2014 (Year of publication)
Showing 151 - 200 of 681
Showing 151 - 200 of 681
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140207 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration IAF |
Pook M | 2014 | 33% | |
Study summaryFull title
All authors
Pook M, Tamming L, Padari K, Tiido T, Maimets T, Patarroyo M, Juronen E, Jaks V, Ingerpuu S
Journal
J Thromb Haemost
Abstract
BACKGROUND: Blood platelets secrete upon activation of laminins 411/421 and 511/521, large adhesive (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
microvesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration IAF Adj. k-factor
255.8 (pelleting)
Protein markers
EV: CD63/ CD41/ CD62P/ CD61
non-EV: Proteomics
no
Show all info
Study aim
Other/Laminin secretion
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW41
Pelleting: adjusted k-factor
255.8
Filtration steps
0.2µm > x > 0.1µm
Immunoaffinity capture
Selected surface protein(s)
CD63
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD41/ CD61/ CD62P
ELISA
Detected EV-associated proteins
CD41/ CD61/ CD62P
|
||||||||
EV140035 | 2/2 | Homo sapiens | Serum |
(d)(U)C Filtration |
Melo SA | 2014 | 33% | |
Study summaryFull title
All authors
Melo SA, Sugimoto H, O'Connell JT, Kato N, Villanueva A, Vidal A, Qiu L, Vitkin E, Perelman LT, Melo CA, Lucci A, Ivan C, Calin GA, Kalluri R
Journal
Cancer Cell
Abstract
Exosomes are secreted by all cell types and contain proteins and nucleic acids. Here, we report that (show more...)
EV-METRIC
33% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
276.6 (pelleting) / 276.6 (washing)
Protein markers
EV: Flotilin1
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW40
Pelleting: adjusted k-factor
276.6
Wash: Rotor Type
SW40
Wash: adjusted k-factor
276.6
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Flotilin1
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM/ atomic force EM
Image type
Close-up
|
||||||||
EV140052 | 1/2 | Homo sapiens | NAY |
(d)(U)C DC Filtration |
Lundholm M | 2014 | 33% | |
Study summaryFull title
All authors
Lundholm M, Schröder M, Nagaeva O, Baranov V, Widmark A, Mincheva-Nilsson L, Wikström P
Journal
PLoS One
Abstract
Tumor-derived exosomes, which are nanometer-sized extracellular vesicles of endosomal origin, have e (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC Filtration Protein markers
EV: TSG101/ PSMA/ CD63
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ TSG101/ PSMA
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
PSMA
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140033 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration UF |
Liu C | 2014 | 33% | |
Study summaryFull title
All authors
Liu C, Qu L, Lian S, Tian Z, Zhao C, Meng L, Shou C
Journal
FEBS J
Abstract
Synuclein-? (SNCG) is a chaperone protein and exists mainly in the cytoplasm. SNCG confers chemoresi (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Protein markers
EV: HSP90/ GAPDH/ Alix/ Annexin2/ LAMP1/ Flotillin2/ HSP70
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Other/Synuclein-gamma secretion
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ HSP90/ HSP70/ "Flotillin2/ LAMP1/ Annexin2/ GAPDH"
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
"Flotillin2/ LAMP1/ Annexin2/ GAPDH"
Characterization: Particle analysis
None
|
||||||||
EV140184 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG UF |
Kuruppu S | 2014 | 33% | |
Study summaryFull title
All authors
Kuruppu S, Rajapakse NW, Dunstan RA, Smith AI
Journal
Mol Cell Biochem
Abstract
This study examined the effect of nitric oxide on the production of soluble ECE-1. Activity of ECE-1 (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
DG UF Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.38-1.4
Show all info
Study aim
Other/ECE-1 release
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Density gradient
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Characterization: Particle analysis
None
|
||||||||
EV140181 | 1/1 | Homo sapiens | Blood plasma |
(d)(U)C DG |
Konadu KA | 2014 | 33% | |
Study summaryFull title
All authors
Konadu KA, Chu J, Huang MB, Amancha PK, Armstrong W, Powell MD, Villinger F, Bond VC
Journal
J Infect Dis
Abstract
Human immunodeficiency virus (HIV)-infected and viremic individuals exhibit elevated levels of plasm (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: CD45/ AChE/ CD63/ CD9
non-EV: p24 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Density gradient
Speed (g)
400000
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD9/ CD45/ AChE
Detected contaminants
p24
ELISA
Detected EV-associated proteins
CD45/ AChE
Characterization: Particle analysis
EM
EM-type
immune EM
EM protein
CD63
Image type
Close-up
|
||||||||
EV140028 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration |
Jaworski E | 2014 | 33% | |
Study summaryFull title
All authors
Jaworski E, Narayanan A, Van Duyne R, Shabbeer-Meyering S, Iordanskiy S, Saifuddin M, Das R, Afonso PV, Sampey GC, Chung M, Popratiloff A, Shrestha B, Sehgal M, Jain P, Vertes A, Mahieux R, Kashanchi F
Journal
J Biol Chem
Abstract
Human T-lymphotropic virus type 1 (HTLV-1) is the causative agent of adult T-cell leukemia and HTLV- (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: Alix/ HSP70/ Beta-actin/ CD63
non-EV: Cell organelle protein Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Wash: volume per pellet (ml)
22
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ HSP70/ Beta-actin
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
Beta-actin
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV140273 | 2/2 | Homo sapiens | NAY |
Filtration Tangential flow filter and track etch filter UF |
Heinemann ML | 2014 | 33% | |
Study summaryFull title
All authors
Heinemann ML, Ilmer M, Silva LP, Hawke DH, Recio A, Vorontsova MA, Alt E, Vykoukal J
Journal
J Chromatogr A
Abstract
Early and minimally invasive detection of malignant events or other pathologies is of utmost importa (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
Filtration
Tangential flow filter and track etch filter UF Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Filtration steps
0.2µm > x > 0.1µm
Other
Name other separation method
Tangential flow filter and track etch filter
Characterization: Protein analysis
Characterization: Particle analysis
DLS
NTA
EM
EM-type
immune EM
EM protein
CD63
Image type
Close-up
|
||||||||
EV140117 | 3/8 | Homo sapiens | Blood plasma |
IAF Microfluidics |
He M | 2014 | 33% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF
Microfluidics Protein markers
EV:
non-EV: Proteomics
no
TEM measurements
50-100
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
CD81
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
Report size (nm)
50-100
|
||||||||
EV140117 | 4/8 | Homo sapiens | Blood plasma |
IAF Microfluidics |
He M | 2014 | 33% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF
Microfluidics Protein markers
EV:
non-EV: Proteomics
no
TEM measurements
25-100
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
CD9
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
Report size (nm)
25-100
|
||||||||
EV140117 | 8/8 | Homo sapiens | Blood plasma |
IAF Microfluidics |
He M | 2014 | 33% | |
Study summaryFull title
All authors
He M, Crow J, Roth M, Zeng Y, Godwin AK
Journal
Lab Chip
Abstract
Developing blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF
Microfluidics Protein markers
EV:
non-EV: Proteomics
no
TEM measurements
50-100
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
α-IGF-1R
Other
Name other separation method
Microfluidics
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
Report size (nm)
50-100
|
||||||||
EV140078 | 1/1 | Mus musculus | NAY |
(d)(U)C Filtration |
Guo BB | 2014 | 33% | |
Study summaryFull title
All authors
Guo BB, Bellingham SA, Hill AF
Journal
J Biol Chem
Abstract
Prion diseases are a group of transmissible, fatal neurodegenerative disorders associated with the m (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: TSG101/ AChE/ Flotilin1
non-EV: NUCLEOPORIN/ Cell organelle protein/ Bcl2 Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Flotilin1/ TSG101/ AChE
Detected contaminants
Cell organelle protein/ "Bcl2/ NUCLEOPORIN"
ELISA
Detected EV-associated proteins
AChE
Characterization: Particle analysis
TRPS
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140075 | 1/1 | Mus musculus | NAY |
(d)(U)C DG |
Glebov K | 2014 | 33% | |
Study summaryFull title
All authors
Glebov K, Löchner M, Jabs R, Lau T, Merkel O, Schloss P, Steinhäuser C, Walter J
Journal
Glia
Abstract
Microglia are resident immune cells in the brain and exert important functions in the regulation of (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Alix/ Beta-actin/ Flotilin1
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Bottom-up
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ Flotilin1/ Beta-actin
ELISA
Detected EV-associated proteins
Beta-actin
Characterization: Particle analysis
None
|
||||||||
EV140073 | 1/1 | Homo sapiens | Blood plasma |
(d)(U)C ExoQuick Filtration |
Ge Q | 2014 | 33% | |
Study summaryFull title
All authors
Ge Q, Zhou Y, Lu J, Bai Y, Xie X, Lu Z
Journal
Molecules
Abstract
Exosomes are small membrane-bound vesicles secreted by most cell types. Exosomes contain various fun (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Filtration Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140072 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration |
Franquesa M | 2014 | 33% | |
Study summaryFull title
All authors
Franquesa M, Hoogduijn MJ, Ripoll E, Luk F, Salih M, Betjes MG, Torras J, Baan CC, Grinyó JM, Merino AM
Journal
Front Immunol
Abstract
The research field on extracellular vesicles (EV) has rapidly expanded in recent years due to the th (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
156.9 (pelleting) / 156.9 (washing)
Protein markers
EV: CD81/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
156.9
Wash: volume per pellet (ml)
39
Wash: Rotor Type
70Ti
Wash: adjusted k-factor
156.9
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD81/ CD9
ELISA
Detected EV-associated proteins
CD63/ CD81/ CD9
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140071 | 1/1 | Mus musculus | NAY |
(d)(U)C Filtration |
Forterre A | 2014 | 33% | |
Study summaryFull title
All authors
Forterre A, Jalabert A, Chikh K, Pesenti S, Euthine V, Granjon A, Errazuriz E, Lefai E, Vidal H, Rome S
Journal
Cell Cycle
Abstract
It has recently been established that exosomes can mediate intercellular cross-talk under normal and (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
157.1 (pelleting)
Protein markers
EV: Alix/ CD81
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
502Ti
Pelleting: adjusted k-factor
157.1
Wash: volume per pellet (ml)
25
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD81
Characterization: Particle analysis
EM
EM-type
transmission EM/ immune EM
EM protein
CD63
Image type
Close-up, Wide-field
|
||||||||
EV140156 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG |
Feng Z | 2014 | 33% | |
Study summaryFull title
All authors
Feng Z, Li Y, McKnight KL, Hensley L, Lanford RE, Walker CM, Lemon SM
Journal
J Clin Invest
Abstract
Unlike other picornaviruses, hepatitis A virus (HAV) is cloaked in host membranes when released from (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Alix/ AChE/ Flotilin1
non-EV: Proteomics
no
EV density (g/ml)
1.120
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Lowest density fraction
8
Highest density fraction
40
Orientation
Top-down
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ Flotilin1/ AChE
ELISA
Detected EV-associated proteins
AChE
Characterization: Particle analysis
None
|
||||||||
EV140154 | 1/1 | Rattus norvegicus/rattus | NAY | (d)(U)C | Escudero CA | 2014 | 33% | |
Study summaryFull title
All authors
Escudero CA, Lazo OM, Galleguillos C, Parraguez JI, Lopez-Verrilli MA, Cabeza C, Leon L, Saeed U, Retamal C, Gonzalez A, Marzolo MP, Carter BD, Court FA, Bronfman FC
Journal
J Cell Sci
Abstract
The p75 neurotrophin receptor (p75, also known as NGFR) is a multifaceted signalling receptor that r (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
141.1 (pelleting)
Protein markers
EV: CD63/ Na/K ATPase/ Rab5/ B-COP
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Rattus norvegicus/rattus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
P55ST2
Pelleting: adjusted k-factor
141.1
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ "Na/K ATPase/ B-COP/ Rab5"
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
"Na/K ATPase/ B-COP/ Rab5"
Characterization: Particle analysis
EM
EM-type
transmission EM/ immune EM
EM protein
p75
Image type
Close-up
|
||||||||
EV140153 | 2/2 | Homo sapiens | Blood plasma |
(d)(U)C Filtration |
Eldh M | 2014 | 33% | |
Study summaryFull title
All authors
Eldh M, Olofsson Bagge R, Lässer C, Svanvik J, Sjöstrand M, Mattsson J, Lindnér P, Choi DS, Gho YS, Lötvall J
Journal
BMC Cancer
Abstract
BACKGROUND: Uveal melanoma is a tumour arising from melanocytes of the eye, and 30 per cent of these (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
130.7 (pelleting)
Protein markers
EV: CD81/ Melan-A/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
130.7
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD81/ CD9/ Melan-A
ELISA
Detected EV-associated proteins
Melan-A
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
EM
EM-type
immune EM
EM protein
CD63
Image type
Close-up
|
||||||||
EV140118 | 3/3 | Sus scrofa | NAY |
(d)(U)C Filtration |
Comelli L | 2014 | 33% | |
Study summaryFull title
All authors
Comelli L, Rocchiccioli S, Smirni S, Salvetti A, Signore G, Citti L, Trivella MG, Cecchettini A
Journal
Mol Biosyst
Abstract
The artery medial layer is mainly composed of vascular smooth muscle cells (VSMCs). These cells cont (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: CD81/ HSP70/ TSG101/ Flotilin1
non-EV: Cell organelle protein Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Sus scrofa
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Flotilin1/ HSP70/ TSG101
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140066 | 1/1 | Homo sapiens | "Cerebrospinal fluid" |
(d)(U)C DC UF |
Chiasserini D | 2014 | 33% | |
Study summaryFull title
All authors
Chiasserini D, van Weering JR, Piersma SR, Pham TV, Malekzadeh A, Teunissen CE, de Wit H, Jiménez CR
Journal
J Proteomics
Abstract
Extracellular vesicles (EVs) are present in human cerebrospinal fluid (CSF), yet little is known abo (show more...)
EV-METRIC
33% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
"Cerebrospinal fluid"
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
DC UF Adj. k-factor
276.6 (pelleting) / 276.6 (washing)
Protein markers
EV: Alix/ Flotilin1/ CD63/ Syntenin
non-EV: Albumin Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
"Cerebrospinal fluid"
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW40
Pelleting: adjusted k-factor
276.6
Wash: Rotor Type
SW40
Wash: adjusted k-factor
276.6
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ Flotilin1/ Syntenin
Detected contaminants
Albumin
Characterization: Particle analysis
EM
EM-type
immune EM
EM protein
CD63
Image type
Close-up
|
||||||||
EV140065 | 1/2 | Homo sapiens | Serum | (d)(U)C | Cheng L | 2014 | 33% | |
Study summaryFull title
All authors
Cheng L, Sharples RA, Scicluna BJ, Hill AF
Journal
J Extracell Vesicles
Abstract
INTRODUCTION: microRNA (miRNA) are small non-coding RNA species that are transcriptionally processed (show more...)
EV-METRIC
33% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
122.2 (pelleting)
Protein markers
EV: TF/ CD63/ Flotillin/ PrP/ HSP70
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70.1Ti
Pelleting: adjusted k-factor
122.2
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ HSP70/ PrP/ TF/ Flotillin
ELISA
Detected EV-associated proteins
PrP/ TF/ Flotillin
Characterization: Particle analysis
TRPS
EM
EM-type
transmission EM
|
||||||||
EV140065 | 2/2 | Homo sapiens | Blood plasma | (d)(U)C | Cheng L | 2014 | 33% | |
Study summaryFull title
All authors
Cheng L, Sharples RA, Scicluna BJ, Hill AF
Journal
J Extracell Vesicles
Abstract
INTRODUCTION: microRNA (miRNA) are small non-coding RNA species that are transcriptionally processed (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
122.2 (pelleting)
Protein markers
EV: TF/ CD63/ Flotillin/ PrP/ HSP70
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70.1Ti
Pelleting: adjusted k-factor
122.2
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ HSP70/ PrP/ TF/ Flotillin
ELISA
Detected EV-associated proteins
PrP/ TF/ Flotillin
Characterization: Particle analysis
TRPS
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140062 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration |
Bronisz A | 2014 | 33% | |
Study summaryFull title
All authors
Bronisz A, Wang Y, Nowicki MO, Peruzzi P, Ansari KI, Ogawa D, Balaj L, De Rienzo G, Mineo M, Nakano I, Ostrowski MC, Hochberg F, Weissleder R, Lawler SE, Chiocca EA, Godlewski J
Journal
Cancer Res
Abstract
Extracellular vesicles have emerged as important mediators of intercellular communication in cancer, (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: YWHAZ/ CD63/ Annexin2/ FASN/ CD9
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD9/ Annexin2/ FASN/ YWHAZ
ELISA
Detected EV-associated proteins
Annexin2/ FASN/ YWHAZ
Characterization: Particle analysis
NTA
EM
EM-type
immune EM
EM protein
CD63
Image type
Close-up, Wide-field
|
||||||||
EV140022 | 1/1 | Homo sapiens | NAY | (d)(U)C | Boelens MC | 2014 | 33% | |
Study summaryFull title
All authors
Boelens MC, Wu TJ, Nabet BY, Xu B, Qiu Y, Yoon T, Azzam DJ, Twyman-Saint Victor C, Wiemann BZ, Ishwaran H, Ter Brugge PJ, Jonkers J, Slingerland J, Minn AJ
Journal
Cell
Abstract
Stromal communication with cancer cells can influence treatment response. We show that stromal and b (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ CD81/ Flotilin1/ Annexin5/ ICAM1/ EpCam/ Beta-actin
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Flotilin1/ TSG101/ ICAM1/ EpCam/ Annexin5/ Beta-actin
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
ICAM1/ EpCam/ Annexin5/ Beta-actin
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV140061 | 1/1 | Homo sapiens | NAY | (d)(U)C | Bian S | 2014 | 33% | |
Study summaryFull title
All authors
Bian S, Zhang L, Duan L, Wang X, Min Y, Yu H
Journal
J Mol Med (Berl)
Abstract
Mesenchymal stem cells (MSCs) have been increasingly tested experimentally and clinically for cardia (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD81/ CD63
non-EV: CD29/ CD73/ CD44/ CD45/ CD31/ Albumin Proteomics
no
TEM measurements
106+-31 (47-180)
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD81
Detected contaminants
Albumin/ "CD29/ CD44/ CD73/ CD31/ CD45"
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
Report size (nm)
106+-31 (47-180)
|
||||||||
EV140060 | 1/1 | Rattus norvegicus/rattus | Blood plasma | ExoQuick | Beninson LA | 2014 | 33% | |
Study summaryFull title
All authors
Beninson LA, Brown PN, Loughridge AB, Saludes JP, Maslanik T, Hills AK, Woodworth T, Craig W, Yin H, Fleshner M
Journal
PLoS One
Abstract
Exosomes, biologically active nanoparticles (40-100 nm) released by hematopoietic and non-hematopoie (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: Rab5a/ HSP72/ CD63
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Rattus norvegicus/rattus
Sample Type
Blood plasma
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
HSP72/ Rab5a
ELISA
Detected EV-associated proteins
CD63/ HSP72/ Rab5a
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140121 | 1/2 | Homo sapiens | NAY | (d)(U)C | Atay S | 2014 | 33% | |
Study summaryFull title
All authors
Atay S, Banskota S, Crow J, Sethi G, Rink L, Godwin AK
Journal
Proc Natl Acad Sci U S A
Abstract
During tumor development, constant interplay occurs between tumor cells and surrounding stromal cell (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ Alix/ CD63/ Flotillin/ Annexin1/ CD9
non-EV: Beta-actin/ Cell organelle protein Proteomics
no
TEM measurements
133+-13;183+-27
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ CD9/ TSG101/ Annexin1/ Flotillin
Detected contaminants
Cell organelle protein/ Beta-actin
ELISA
Detected EV-associated proteins
Annexin1/ Flotillin
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
Report size (nm)
133+-13;183+-27
|
||||||||
EV140057 | 1/2 | Mus musculus | NAY |
(d)(U)C Filtration |
Aswad H | 2014 | 33% | |
Study summaryFull title
All authors
Aswad H, Forterre A, Wiklander OP, Vial G, Danty-Berger E, Jalabert A, Lamazière A, Meugnier E, Pesenti S, Ott C, Chikh K, El-Andaloussi S, Vidal H, Lefai E, Rieusset J, Rome S
Journal
Diabetologia
Abstract
AIMS/HYPOTHESIS: Exosomes released from cells can transfer both functional proteins and RNAs between (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
157.1 (pelleting) / 157.1 (washing)
Protein markers
EV: Alix/ TSG101
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
502Ti
Pelleting: adjusted k-factor
157.1
Wash: volume per pellet (ml)
25
Wash: Rotor Type
50.2Ti
Wash: adjusted k-factor
157.1
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ TSG101
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140057 | 2/2 | Mus musculus | Mouse muscle |
(d)(U)C Filtration |
Aswad H | 2014 | 33% | |
Study summaryFull title
All authors
Aswad H, Forterre A, Wiklander OP, Vial G, Danty-Berger E, Jalabert A, Lamazière A, Meugnier E, Pesenti S, Ott C, Chikh K, El-Andaloussi S, Vidal H, Lefai E, Rieusset J, Rome S
Journal
Diabetologia
Abstract
AIMS/HYPOTHESIS: Exosomes released from cells can transfer both functional proteins and RNAs between (show more...)
EV-METRIC
33% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Mouse muscle
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
157.1 (pelleting) / 157.1 (washing)
Protein markers
EV: Alix/ TSG101
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Mouse muscle
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
502Ti
Pelleting: adjusted k-factor
157.1
Wash: volume per pellet (ml)
25
Wash: Rotor Type
50.2Ti
Wash: adjusted k-factor
157.1
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ TSG101
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140021 | 1/1 | Homo sapiens | NAY | (d)(U)C | Amorim M | 2014 | 33% | |
Study summaryFull title
All authors
Amorim M, Fernandes G, Oliveira P, Martins-de-Souza D, Dias-Neto E, Nunes D
Journal
Proteomics
Abstract
ERBB2/HER2 amplification activates signaling cascades that lead to a tumor cell phenotype. However, (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: HSP70/ Rab27B/ Flotilin1
non-EV: Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Flotilin1/ HSP70/ Rab27B
ELISA
Detected EV-associated proteins
Rab27B
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140019 | 1/1 | Sus scrofa | NAY |
(d)(U)C Filtration UF |
Aboul Naga SH | 2014 | 33% | |
Study summaryFull title
All authors
Aboul Naga SH, Dithmer M, Chitadze G, Kabelitz D, Lucius R, Roider J, Klettner A
Journal
Exp Eye Res
Abstract
The anti-VEGF antibody bevacizumab is widely used off-label for the treatment of various ocular dise (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Protein markers
EV: TSG101/ CD63
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Sus scrofa
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ TSG101
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140290 | 2/3 | Homo sapiens | NAY |
(d)(U)C UF |
van der Mijn JC | 2014 | 29% | |
Study summaryFull title
All authors
van der Mijn JC, Sol N, Mellema W, Jimenez CR, Piersma SR, Dekker H, Schutte LM, Smit EF, Broxterman HJ, Skog J, Tannous BA, Wurdinger T, Verheul HM
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Extracellular vesicles (EVs) are small nanometre-sized vesicles that are circulating in (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
UF Adj. k-factor
256 (pelleting)
Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
SW32
Pelleting: adjusted k-factor
256.0
Characterization: Protein analysis
Characterization: Particle analysis
None
|
||||||||
EV140258 | 3/3 | Mus musculus | Serum |
(d)(U)C DG Filtration |
Ridder K | 2014 | 29% | |
Study summaryFull title
All authors
Ridder K, Keller S, Dams M, Rupp AK, Schlaudraff J, Del Turco D, Starmann J, Macas J, Karpova D, Devraj K, Depboylu C, Landfried B, Arnold B, Plate KH, Höglinger G, Sültmann H, Altevogt P, Momma S
Journal
PLoS Biol
Abstract
Mechanisms behind how the immune system signals to the brain in response to systemic inflammation ar (show more...)
EV-METRIC
29% (72nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
97.39 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW40
Pelleting: adjusted k-factor
97.39
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
21
Orientation
Top-down
Rotor type
SW40
Filtration steps
0.22µm or 0.2µm
Characterization: Particle analysis
None
|
||||||||
EV140129 | 1/2 | Homo sapiens | NAY | (d)(U)C | Ma X | 2014 | 29% | |
Study summaryFull title
All authors
Ma X, Chen Z, Hua D, He D, Wang L, Zhang P, Wang J, Cai Y, Gao C, Zhang X, Zhang F, Wang T, Hong T, Jin L, Qi X, Chen S, Gu X, Yang D, Pan Q, Zhu Y, Chen Y, Chen D, Jiang L, Han X, Zhang Y, Jin J, Yao X
Journal
Proc Natl Acad Sci U S A
Abstract
A critical challenge for chemotherapy is the development of chemoresistance in breast cancer. Howeve (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
EM
EM-type
transmission EM/ immune EM/ scanning EM
EM protein
Adriamycin; TrpC5; Flotillin2
Image type
Close-up, Wide-field
Report size (nm)
Not reported
|
||||||||
EV140252 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration filter |
L?rincz ÁM | 2014 | 29% | |
Study summaryFull title
All authors
Lorincz ÁM, Timár CI, Marosvári KA, Veres DS, Otrokocsi L, Kittel Á, Ligeti E
Journal
J Extracell Vesicles
Abstract
AIM: To carry out a systematic study on the effect of different storage conditions on the number as (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration filter Adj. k-factor
373.5 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
10
Pelleting: rotor type
Hermle Z216MK
Pelleting: adjusted k-factor
373.5
Other
Name other separation method
filter
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140105 | 1/1 | Mycobacterium tuberculosis | Bacteria |
(d)(U)C DG Filtration UF |
Prados-Rosales R | 2014 | 29% | |
Study summaryFull title
All authors
Prados-Rosales R, Weinrick BC, Piqué DG, Jacobs WR Jr, Casadevall A, Rodriguez GM
Journal
J Bacteriol
Abstract
Mycobacterium tuberculosis releases membrane vesicles packed with molecules that can modulate the im (show more...)
EV-METRIC
29% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
NAY
Focus vesicles
Membrane(-derived) vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Filtration UF Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mycobacterium tuberculosis
Sample Type
Bacteria
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Lowest density fraction
5
Highest density fraction
30
Orientation
Bottom-up
Speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140235 | 1/1 | Homo sapiens | NAY | (d)(U)C | Xu JF | 2014 | 29% | |
Study summaryFull title
All authors
Xu JF, Yang GH, Pan XH, Zhang SJ, Zhao C, Qiu BS, Gu HF, Hong JF, Cao L, Chen Y, Xia B, Bi Q, Wang YP
Journal
PLoS One
Abstract
The physiological role of microRNAs (miRNAs) in osteoblast differentiation remains elusive. Exosomal (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
396.8 (pelleting) / 167.3 (washing)
Protein markers
EV: CD63/ CD86/ MHC2/ CD54/ MHC1
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
110
Pelleting: rotor type
SW28
Pelleting: adjusted k-factor
396.8
Wash: Rotor Type
SW60
Wash: adjusted k-factor
167.3
Western Blot
Detected EV-associated proteins
MHC2/ MHC1/ CD86/ CD54
ELISA
Detected EV-associated proteins
MHC2/ MHC1/ CD86/ CD54
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
None
|
||||||||
EV140393 | 1/1 | Mus musculus | NAY |
(d)(U)C Filtration UF |
Sobo-Vujanovic A | 2014 | 29% | |
Study summaryFull title
All authors
Sobo-Vujanovic A, Munich S, Vujanovic NL
Journal
Cell Immunol
Abstract
Dendritic cells (DCs) are the major sentinel, antigen-presenting and regulatory components of the im (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Adj. k-factor
126 (pelleting)
Protein markers
EV: CD14/ MHC2/ TLR4
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
90Ti
Pelleting: adjusted k-factor
126
Filtration steps
0.45µm > x > 0.22µm,
Western Blot
Detected EV-associated proteins
MHC2/ CD14/ TLR4
ELISA
Detected EV-associated proteins
MHC2/ CD14/ TLR4
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
None
|
||||||||
EV140283 | 2/2 | Homo sapiens | Ascites |
(d)(U)C DG UF |
Shender VO | 2014 | 29% | |
Study summaryFull title
All authors
Shender VO, Pavlyukov MS, Ziganshin RH, Arapidi GP, Kovalchuk SI, Anikanov NA, Altukhov IA, Alexeev DG, Butenko IO, Shavarda AL, Khomyakova EB, Evtushenko E, Ashrafyan LA, Antonova IB, Kuznetcov IN, Gorbachev AY, Shakhparonov MI, Govorun VM
Journal
Mol Cell Proteomics
Abstract
Ovarian cancer ascites is a native medium for cancer cells that allows investigation of their secret (show more...)
EV-METRIC
29% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Ascites
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
DG UF Adj. k-factor
159.6 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Ascites
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
60Ti
Pelleting: adjusted k-factor
159.6
Density gradient
Lowest density fraction
15
Highest density fraction
48
Orientation
Bottom-up
Speed (g)
200000
Characterization: Particle analysis
NTA
|
||||||||
EV140282 | 1/2 | Mus musculus | NAY |
(d)(U)C DC Filtration |
Saunderson SC | 2014 | 29% | |
Study summaryFull title
All authors
Saunderson SC, Dunn AC, Crocker PR, McLellan AD
Journal
Blood
Abstract
Exosomes are lipid nanovesicles released following fusion of the endosoma limiting membrane with the (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC Filtration Protein markers
EV: MHC2/ CD9
non-EV: a2,3-sialic acid/ CD21/ CD24/ CD19/ a2,6-sialic acid/ Ig Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
MHC2
ELISA
Detected EV-associated proteins
CD9/ MHC2
Detected contaminants
"CD19/ CD21/ CD24/ Ig/ a2,3-sialic acid/ a2,6-sialic acid"
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140269 | 2/3 | Homo sapiens | Brain tissue |
(d)(U)C DG Filtration |
Saman S | 2014 | 29% | |
Study summaryFull title
All authors
Saman S, Lee NC, Inoyo I, Jin J, Li Z, Doyle T, McKee AC, Hall GF
Journal
J Alzheimers Dis
Abstract
Tau misprocessing to form aggregates and other toxic species has emerged as a major feature in our d (show more...)
EV-METRIC
29% (29th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Brain tissue
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Brain tissue
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Characterization: Protein analysis
Characterization: Particle analysis
None
|
||||||||
EV140269 | 3/3 | Homo sapiens | "Cerebrospinal fluid" |
(d)(U)C DG |
Saman S | 2014 | 29% | |
Study summaryFull title
All authors
Saman S, Lee NC, Inoyo I, Jin J, Li Z, Doyle T, McKee AC, Hall GF
Journal
J Alzheimers Dis
Abstract
Tau misprocessing to form aggregates and other toxic species has emerged as a major feature in our d (show more...)
EV-METRIC
29% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
"Cerebrospinal fluid"
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
"Cerebrospinal fluid"
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Characterization: Protein analysis
Characterization: Particle analysis
None
|
||||||||
EV140211 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG Filtration |
Rupert DL | 2014 | 29% | |
Study summaryFull title
All authors
Rupert DL, Lässer C, Eldh M, Block S, Zhdanov VP, Lotvall JO, Bally M, Höök F
Journal
Anal Chem
Abstract
Exosomes are cell-secreted nanometer-sized extracellular vesicles that have been reported to play an (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.24-1.31
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Density gradient
Only used for validation of main results
Yes
Filtration steps
0.22µm or 0.2µm
Characterization: Particle analysis
NTA
|
||||||||
EV140280 | 1/2 | Mus musculus | NAY |
(d)(U)C IAF |
Rahman MJ | 2014 | 29% | |
Study summaryFull title
All authors
Rahman MJ, Regn D, Bashratyan R, Dai YD
Journal
Diabetes
Abstract
Exosomes (EXOs) are secreted, nano-sized membrane vesicles that contain potent immunostimulatory mat (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
IAF Protein markers
EV: CD81/ CD63
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Immunoaffinity capture
Selected surface protein(s)
CD63
Characterization: Protein analysis
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140205 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG Filtration |
Philip R | 2014 | 29% | |
Study summaryFull title
All authors
Philip R, Heiler S, Mu W, Büchler MW, Zöller M, Thuma F
Journal
Oncotarget
Abstract
In colorectal cancer (CoCa) EpCAM is frequently associated with claudin-7. There is evidence that tu (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.17
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Density gradient
Lowest density fraction
5
Highest density fraction
40
Orientation
Bottom-up
Speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Particle analysis
None
|
||||||||
EV140089 | 1/1 | Mus musculus | NAY |
(d)(U)C UF |
Petersen KE | 2014 | 29% | |
Study summaryFull title
All authors
Petersen KE, Manangon E, Hood JL, Wickline SA, Fernandez DP, Johnson WP, Gale BK
Journal
Anal Bioanal Chem
Abstract
Exosomes participate in cancer metastasis, but studying them presents unique challenges as a result (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV140087 | 1/2 | Vitis Citrus paradisi |
Plant material (grape, grapefruit) |
(d)(U)C DG |
Mu J | 2014 | 29% | |
Study summaryFull title
All authors
Mu J, Zhuang X, Wang Q, Jiang H, Deng ZB, Wang B, Zhang L, Kakar S, Jun Y, Miller D, Zhang HG
Journal
Mol Nutr Food Res
Abstract
SCOPE: Exosomes, small vesicles participating in intercellular communication, have been extensively (show more...)
EV-METRIC
29% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Plant material (grape, grapefruit)
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Vitis / Citrus paradisi
Sample Type
Plant material (grape, grapefruit)
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Density gradient
Lowest density fraction
8
Highest density fraction
60
Orientation
Top-down
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Wide-field
|
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EV140087 | 2/2 | Zingiber officinale Daucus carota |
Plant material (ginger, carrot) |
(d)(U)C DG |
Mu J | 2014 | 29% | |
Study summaryFull title
All authors
Mu J, Zhuang X, Wang Q, Jiang H, Deng ZB, Wang B, Zhang L, Kakar S, Jun Y, Miller D, Zhang HG
Journal
Mol Nutr Food Res
Abstract
SCOPE: Exosomes, small vesicles participating in intercellular communication, have been extensively (show more...)
EV-METRIC
29% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Plant material (ginger, carrot)
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Zingiber officinale / Daucus carota
Sample Type
Plant material (ginger, carrot)
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Density gradient
Lowest density fraction
8
Highest density fraction
60
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140086 | 1/1 | Mus musculus | NAY |
(d)(U)C Filtration |
Morishita M | 2014 | 29% | |
Study summaryFull title
All authors
Morishita M, Takahashi Y, Nishikawa M, Sano K, Kato K, Yamashita T, Imai T, Saji H, Takakura Y
Journal
J Pharm Sci
Abstract
We previously succeeded in the visualization of tissue distribution of B16BL6 cells-derived exosomes (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Filtration steps
0.22µm or 0.2µm
Characterization: Particle analysis
NTA
TRPS
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
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