Search > Results
You searched for: EV140235 (EV-TRACK ID)
Showing 1 - 1 of 1
Showing 1 - 1 of 1
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140235 | 1/1 | Homo sapiens | NAY | (d)(U)C | Xu JF | 2014 | 29% | |
Study summaryFull title
All authors
Xu JF, Yang GH, Pan XH, Zhang SJ, Zhao C, Qiu BS, Gu HF, Hong JF, Cao L, Chen Y, Xia B, Bi Q, Wang YP
Journal
PLoS One
Abstract
The physiological role of microRNAs (miRNAs) in osteoblast differentiation remains elusive. Exosomal (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
396.8 (pelleting) / 167.3 (washing)
Protein markers
EV: CD63/ CD86/ MHC2/ CD54/ MHC1
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
110
Pelleting: rotor type
SW28
Pelleting: adjusted k-factor
396.8
Wash: Rotor Type
SW60
Wash: adjusted k-factor
167.3
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
MHC2/ MHC1/ CD86/ CD54
ELISA
Antibody details provided?
No
Detected EV-associated proteins
MHC2/ MHC1/ CD86/ CD54
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Selected surface protein(s)
Yes
|
||||||||
1 - 1 of 1 |
EV-TRACK ID | EV140235 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | NAY |
condition | NAY |
separation protocol | (d)(U)C |
Exp. nr. | 1 |
EV-METRIC % | 29 |