Search > Results
You searched for: 2024 (Year of publication)
Showing 1 - 50 of 70
Showing 1 - 50 of 70
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV230597 | 1/9 | Homo sapiens | ESC |
(d)(U)C DG |
Chen Z | 2024 | 100% | |
Study summaryFull title
All authors
Chen Z, Luo L, Ye T, Zhou J, Niu X, Yuan J, Yuan T, Fu D, Li H, Li Q, Wang Y
Journal
J Extracell Vesicles
Abstract
Pluripotent stem cell-derived small extracellular vesicles (PSC-sEVs) have demonstrated great clinic (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Adj. k-factor
37432 (washing)
Protein markers
EV: Alix/ CD9/ CD63/ TSG101/ CD81
non-EV: GM130/ Calnexin Proteomics
yes
EV density (g/ml)
1.17
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
ESC
EV-harvesting Medium
Serum free medium
Cell count
2.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
25
Wash: time (min)
70
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
100000
Wash: adjusted k-factor
37432
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
20%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
11
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
150000
Duration (min)
600
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
10
Pelleting: speed (g)
150000
Pelleting: adjusted k-factor
10018
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ CD63/ TSG101
Not detected contaminants
GM130/ Calnexin
Flow cytometry
Type of Flow cytometry
Nano-flow Cytometry
Hardware adaptation to ~100nm EV's
By utilizing Rayleigh scattering
Calibration bead size
0.25
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Proteomics database
ProteomeXchange
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
Nano-flow Cytometry
Hardware adjustment
By utilizing Rayleigh scattering
Calibration bead size
68/ 91,113,155
Report type
Size range/distribution
Reported size (nm)
40-150
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 2.03E+08
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
100
|
||||||||
EV230597 | 2/9 | Homo sapiens | iPSC |
(d)(U)C DG |
Chen Z | 2024 | 100% | |
Study summaryFull title
All authors
Chen Z, Luo L, Ye T, Zhou J, Niu X, Yuan J, Yuan T, Fu D, Li H, Li Q, Wang Y
Journal
J Extracell Vesicles
Abstract
Pluripotent stem cell-derived small extracellular vesicles (PSC-sEVs) have demonstrated great clinic (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Adj. k-factor
37432 (washing)
Protein markers
EV: TSG101/ CD9/ CD63/ CD81
non-EV: None Proteomics
yes
EV density (g/ml)
1.17
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
iPSC
EV-harvesting Medium
Serum free medium
Cell count
2.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
25
Wash: time (min)
70
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
100000
Wash: adjusted k-factor
37432
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
20%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
11
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
150000
Duration (min)
600
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
10
Pelleting: speed (g)
150000
Pelleting: adjusted k-factor
10018
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ CD63/ TSG101
Not detected contaminants
GM130/ Calnexin
Flow cytometry
Type of Flow cytometry
Nano-flow Cytometry
Hardware adaptation to ~100nm EV's
By utilizing Rayleigh scattering
Calibration bead size
0.25
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Proteomics database
ProteomeXchange
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
Nano-flow Cytometry
Hardware adjustment
By utilizing Rayleigh scattering
Calibration bead size
68/ 91,113,155
Report type
Size range/distribution
Reported size (nm)
40-150
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 2.03E+08
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
100
|
||||||||
EV230372 | 7/14 | Homo sapiens | H2228 |
(d)(U)C DG |
Schöne N | 2024 | 100% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
large EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: Actinin-4/ Rgap1/ Syntenin-1/ CD81/ EMMPRIN/ EpCAM/ EGFR/ Mitofilin,Arf6
non-EV: GM130/ HDAC1/ Albumin/ ApoA1/ ApoB Proteomics
no
EV density (g/ml)
1.10-1.17
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
H2228
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
17000
Wash: volume per pellet (ml)
1
Wash: time (min)
30
Wash: Rotor Type
Heraeus 3331
Wash: speed (g)
17000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
16.5
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
16
Pelleting: speed (g)
100000
Pelleting-wash: volume per pellet (mL)
16
Pelleting-wash: duration (min)
60
Pelleting-wash: speed (g)
SW 32 Ti
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Actinin-4/ Rgap1/ Mitofilin/ Arf6/ CD81
Not detected EV-associated proteins
Syntenin
Not detected contaminants
GM130/ HDAC1/ Albumin/ ApoA1/ ApoB
Flow cytometry
Type of Flow cytometry
standard flow cytometer
Calibration bead size
0.2, 0.8
Antibody details provided?
No
Detected EV-associated proteins
EMMPRIN/ EpCAM/ EGFR
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
196.8
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 4.83E+09
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230372 | 9/14 | Homo sapiens | H596 |
(d)(U)C DG |
Schöne N | 2024 | 100% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
large EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: Actinin-4/ Rgap1/ Syntenin-1/ CD81/ EMMPRIN/ EpCAM/ EGFR/ Mitofilin,Arf6
non-EV: GM130/ HDAC1/ Albumin/ ApoA1/ ApoB Proteomics
no
EV density (g/ml)
1.10-1.17
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
H596
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
17000
Wash: volume per pellet (ml)
1
Wash: time (min)
30
Wash: Rotor Type
Heraeus 3331
Wash: speed (g)
17000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
16.5
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
16
Pelleting: speed (g)
100000
Pelleting-wash: volume per pellet (mL)
16
Pelleting-wash: duration (min)
60
Pelleting-wash: speed (g)
SW 32 Ti
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Actinin-4/ Rgap1/ Mitofilin/ Arf6
Not detected EV-associated proteins
Syntenin/ CD81
Not detected contaminants
GM130/ HDAC1/ Albumin/ ApoA1/ ApoB
Flow cytometry
Type of Flow cytometry
standard flow cytometer
Calibration bead size
0.2, 0.8
Antibody details provided?
No
Detected EV-associated proteins
EMMPRIN/ EpCAM/ EGFR
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
188.7
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.33E+09
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230372 | 13/14 | Homo sapiens | Blood plasma | (d)(U)C | Schöne N | 2024 | 100% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NSCLC
Focus vesicles
large EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Actinin-4/ Rgap1/ EMMPRIN/ EpCAM/ EGFR
non-EV: ApoA1/ ApoB Proteomics
no
EV density (g/ml)
1.10-1.17
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
17000
Wash: volume per pellet (ml)
1
Wash: time (min)
30
Wash: Rotor Type
Heraeus 3331
Wash: speed (g)
17000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
16.5
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
16
Pelleting: speed (g)
100000
Pelleting-wash: volume per pellet (mL)
16
Pelleting-wash: duration (min)
60
Pelleting-wash: speed (g)
SW 32 Ti
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Actinin-4/ Rgap1/ EMMPRIN
Detected contaminants
ApoB
Not detected contaminants
ApoA1
Flow cytometry
Type of Flow cytometry
standard flow cytometer
Calibration bead size
0.2, 0.8
Antibody details provided?
No
Detected EV-associated proteins
EMMPRIN/ EpCAM/ EGFR
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
187
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 6.05E+08
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230372 | 3/14 | Homo sapiens | HCC-78 |
(d)(U)C DG |
Schöne N | 2024 | 78% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
large EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: Actinin-4/ Rgap1/ Syntenin-1/ CD81/ EMMPRIN/ EpCAM/ EGFR/ Mitofilin,Arf6
non-EV: GM130/ HDAC1/ Albumin/ ApoA1/ ApoB Proteomics
no
EV density (g/ml)
1.10-1.17
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HCC-78
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
17000
Wash: volume per pellet (ml)
1
Wash: time (min)
30
Wash: Rotor Type
Heraeus 3331
Wash: speed (g)
17000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
16.5
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
16
Pelleting: speed (g)
100000
Pelleting-wash: volume per pellet (mL)
16
Pelleting-wash: duration (min)
60
Pelleting-wash: speed (g)
SW 32 Ti
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Actinin-4/ Rgap1/ Syntenin-1/ Arf6/ Mitofilin
Not detected EV-associated proteins
CD81
Not detected contaminants
GM130/ HDAC1/ Albumin/ ApoA1/ ApoB
Flow cytometry
Type of Flow cytometry
standard flow cytometer
Calibration bead size
0.2, 0.8
Antibody details provided?
No
Detected EV-associated proteins
EMMPRIN/ EpCAM/ EGFR
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
200.13
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 5.63E+09
|
||||||||
EV230372 | 8/14 | Homo sapiens | H2228 | (d)(U)C | Schöne N | 2024 | 78% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Syntenin/ Actinin-4/ Arf6/ Rgap1/ Mitofilin
non-EV: GM130/ HDAC1/ Albumin/ ApoA1/ ApoB Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
H2228
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
143000
Wash: volume per pellet (ml)
1.4
Wash: time (min)
90
Wash: Rotor Type
TLA-55
Wash: speed (g)
143000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Syntenin/ Arf6/ CD81
Not detected EV-associated proteins
Actinin-4/ Rgap1/ Mitofilin
Not detected contaminants
GM130/ HDAC1/ Albumin/ ApoA1/ ApoB
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
163.8
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 2.10E+10
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230372 | 10/14 | Homo sapiens | H596 |
(d)(U)C DG |
Schöne N | 2024 | 78% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: CD81/ Syntenin/ Actinin-4/ Arf6/ Rgap1/ Mitofilin
non-EV: GM130/ HDAC1/ Albumin/ ApoA1/ ApoB Proteomics
no
EV density (g/ml)
1.10-1.17
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
H596
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
143000
Wash: volume per pellet (ml)
1.4
Wash: time (min)
90
Wash: Rotor Type
TLA-55
Wash: speed (g)
143000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
16.5
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
16
Pelleting: speed (g)
100000
Pelleting-wash: volume per pellet (mL)
16
Pelleting-wash: duration (min)
60
Pelleting-wash: speed (g)
SW 32 Ti
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Syntenin/ Actinin-4/ Arf6/ CD81
Not detected EV-associated proteins
Rgap1/ Mitofilin
Not detected contaminants
GM130/ HDAC1/ Albumin/ ApoA1/ ApoB
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
141.2
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 6.09E+09
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV220326 | 1/4 | Homo sapiens | Glioblastoma stem-like cells NCH421k |
(d)(U)C DC |
Lokumcu T | 2024 | 78% | |
Study summaryFull title
All authors
Lokumcu T, Iskar M, Schneider M, Helm D, Klinke G, Schlicker L, Bethke F, Müller G, Richter K, Poschet G, Phillips E, Goidts V
Journal
ACS Nano
Abstract
Glioblastoma is a deadly brain tumor for which there is no cure. The presence of glioblastoma stem-l (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small extracellular vesicles (sEVs)
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
non-EV: Albumin/ Calreticulin/ Calnexin/ Argonaute-2/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein/ Cytochrome c1/ Lamin B1/ 60S acidic ribosomal protein P0 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Glioblastoma stem-like cells NCH421k
EV-harvesting Medium
Serum free medium
Cell viability (%)
91 - 93
Cell count
194200000 - 222312000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
10
Wash: time (min)
70
Wash: Rotor Type
SW 40 Ti
Wash: speed (g)
100000
Density cushion
Density medium
Iodixanol
Sample volume
7
Cushion volume
4
Density of the cushion
20%
Centrifugation time
70
Centrifugation speed
100000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per million cells
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
Detected contaminants
60S acidic ribosomal protein P0 (RPLP0)
Not detected contaminants
GM130/ Lamin B1/ Cytochrome c
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Calreticulin/ Calnexin
Not detected contaminants
Argonaute 2/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein/ Cytochrome c1
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
175.0
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 4,58E+12
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV220326 | 2/4 | Homo sapiens | Glioblastoma stem-like cells NCH644 |
(d)(U)C DC |
Lokumcu T | 2024 | 78% | |
Study summaryFull title
All authors
Lokumcu T, Iskar M, Schneider M, Helm D, Klinke G, Schlicker L, Bethke F, Müller G, Richter K, Poschet G, Phillips E, Goidts V
Journal
ACS Nano
Abstract
Glioblastoma is a deadly brain tumor for which there is no cure. The presence of glioblastoma stem-l (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small extracellular vesicles (sEVs)
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
non-EV: Albumin/ Calreticulin/ Calnexin/ Argonaute-2/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein/ Cytochrome c1/ Lamin B1/ 60S acidic ribosomal protein P0 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Glioblastoma stem-like cells NCH644
EV-harvesting Medium
Serum free medium
Cell viability (%)
83 - 90
Cell count
225576000 - 231070000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
10
Wash: time (min)
70
Wash: Rotor Type
SW 40 Ti
Wash: speed (g)
100000
Density cushion
Density medium
Iodixanol
Sample volume
7
Cushion volume
4
Density of the cushion
20%
Centrifugation time
70
Centrifugation speed
100000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per million cells
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
Detected contaminants
60S acidic ribosomal protein P0
Not detected contaminants
GM130/ Lamin B1/ Cytochrome c
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Calreticulin/ Calnexin
Not detected contaminants
Argonaute 2/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein/ Cytochrome c1
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
169.7
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1,87E+12
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV220326 | 3/4 | Homo sapiens | Glioblastoma stem-like cells NCH705 |
(d)(U)C DC |
Lokumcu T | 2024 | 78% | |
Study summaryFull title
All authors
Lokumcu T, Iskar M, Schneider M, Helm D, Klinke G, Schlicker L, Bethke F, Müller G, Richter K, Poschet G, Phillips E, Goidts V
Journal
ACS Nano
Abstract
Glioblastoma is a deadly brain tumor for which there is no cure. The presence of glioblastoma stem-l (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small extracellular vesicles (sEVs)
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
non-EV: Albumin/ Calreticulin/ Calnexin/ Argonaute-2/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein/ Cytochrome c1/ Lamin B1/ 60S acidic ribosomal protein P0 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Glioblastoma stem-like cells NCH705
EV-harvesting Medium
Serum free medium
Cell viability (%)
98
Cell count
204312000 - 225840000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
10
Wash: time (min)
70
Wash: Rotor Type
SW 40 Ti
Wash: speed (g)
100000
Density cushion
Density medium
Iodixanol
Sample volume
7
Cushion volume
4
Density of the cushion
20%
Centrifugation time
70
Centrifugation speed
100000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per million cells
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
Detected contaminants
60S acidic ribosomal protein P0 (RPLP0)
Not detected contaminants
GM130/ Lamin B1/ Cytochrome c
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Calreticulin/ Calnexin
Not detected contaminants
Argonaute 2/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein/ Cytochrome c1
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
179.7
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 6,05E+12
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV220326 | 4/4 | Homo sapiens | Glioblastoma stem-like cells NCH711d |
(d)(U)C DC |
Lokumcu T | 2024 | 78% | |
Study summaryFull title
All authors
Lokumcu T, Iskar M, Schneider M, Helm D, Klinke G, Schlicker L, Bethke F, Müller G, Richter K, Poschet G, Phillips E, Goidts V
Journal
ACS Nano
Abstract
Glioblastoma is a deadly brain tumor for which there is no cure. The presence of glioblastoma stem-l (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small extracellular vesicles (sEVs)
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
non-EV: Albumin/ Calreticulin/ Calnexin/ Argonaute-2/ GM130/ PMP70/ Tamm-Horsfall protein/ Cytochrome c1/ Lamin B1/ 60S acidic ribosomal protein P0 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Glioblastoma stem-like cells NCH711d
EV-harvesting Medium
Serum free medium
Cell viability (%)
89 - 91
Cell count
102240000 - 116112000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
10
Wash: time (min)
70
Wash: Rotor Type
SW 40 Ti
Wash: speed (g)
100000
Density cushion
Density medium
Iodixanol
Sample volume
7
Cushion volume
4
Density of the cushion
20%
Centrifugation time
70
Centrifugation speed
100000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per million cells
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
Detected contaminants
60S acidic ribosomal protein P0 (RPLP0)
Not detected contaminants
GM130/ Lamin B1/ Cytochrome c
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Calreticulin/ Calnexin
Not detected contaminants
Argonaute 2/ GM130/ PMP70/ Tamm-Horsfall protein/ Cytochrome c1
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
203.5
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 2,87E+12
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230995 | 1/6 | Homo sapiens | MML-1 | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD63/ CD81/ Flotillin-1
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MML-1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
94
Cell count
50000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
16500
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ Flotillin-1
Detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 761800000
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230995 | 2/6 | Homo sapiens | UM22Bap1+/+ | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD63/ CD81/ Flotillin-1
non-EV: Calnexin/ bovine HBA1c/ bovine CPN1 Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
UM22Bap1+/+
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
94
Cell count
50000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
16500
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ Flotillin-1
Not detected contaminants
Calnexin
ELISA
Antibody details provided?
No
Detected contaminants
bovine HBA1c/ bovine CPN1
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 936666666.7
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230995 | 3/6 | Homo sapiens | UM22Bap1-/- | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Flotillin-1/ CD63
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
UM22Bap1-/-
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
93
Cell count
50000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
16500
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Flotillin-1
Not detected EV-associated proteins
CD63
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 36277777.78
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230995 | 4/6 | Homo sapiens | MML-1 | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD63/ CD81/ Flotillin-1
non-EV: Calnexin/ bovine CPN1 Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MML-1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
94
Cell count
50000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ Flotillin-1
Not detected contaminants
Calnexin
ELISA
Antibody details provided?
No
Detected contaminants
bovine CPN1
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 139727777.8
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230995 | 5/6 | Homo sapiens | UM22Bap1+/+ | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD63/ CD81/ Flotillin-1
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
UM22Bap1+/+
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
94
Cell count
50000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ Flotillin-1
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 82011111.11
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230995 | 6/6 | Homo sapiens | UM22Bap1-/- | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD63/ CD81/ Flotillin-1
non-EV: Calnexin/ bovine CPN1 Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
UM22Bap1-/-
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
93
Cell count
50000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ Flotillin-1
Not detected contaminants
Calnexin
ELISA
Antibody details provided?
No
Detected contaminants
bovine CPN1
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 342605555.6
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230994 | 2/6 | Bos taurus | Commercially available FBS | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Commercially available FBS
Sample origin
HI-before EV-depl
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: HSP70/ HSP90/ HBA1
non-EV: Albumin/ Argonaute-2/ Calreticulin/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Bos taurus
Sample Type
Commercially available FBS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
16500
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
HSP70/ HSP90/ HBA1
Proteomics database
No
Detected contaminants
Albumin/ Argonaute-2/ Calreticulin
Not detected contaminants
GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 32260000
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230994 | 4/6 | Bos taurus | Commercially available FBS | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Commercially available FBS
Sample origin
no-HI
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: HSP70/ HSP90/ HBA1
non-EV: Albumin/ Argonaute-2/ Calreticulin/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Bos taurus
Sample Type
Commercially available FBS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
HSP70/ HSP90/ HBA1
Proteomics database
No
Detected contaminants
Albumin/ Argonaute-2/ Calreticulin
Not detected contaminants
GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 148220000
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230994 | 6/6 | Bos taurus | Commercially available FBS | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Commercially available FBS
Sample origin
HI-after EV-depl
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: HSP70/ HSP90/ HBA1
non-EV: Albumin/ Argonaute-2/ Calreticulin/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Bos taurus
Sample Type
Commercially available FBS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
HSP70/ HSP90/ HBA1
Proteomics database
No
Detected contaminants
Albumin/ Argonaute-2/ Calreticulin
Not detected contaminants
GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 133940000
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230572 | 3/6 | Homo sapiens | HEK293-GFP | (d)(U)C | Djeungoue-Petga, Marie-Ange | 2024 | 67% | |
Study summaryFull title
All authors
Marie Ange Djeungoue Petgaa, Catherine Taylora, Alexander Macpherson, Surendar Reddy Dhadi, Thomas Rollin, Jeremy W. Roya, Anirban Ghosh, Stephen M. Lewis, Rodney J. Ouellette
Journal
Abstract
Extracellular vesicles (EVs) are gaining interest as efficient, biocompatible vehicles for cellular (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
GFP overexpression
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD9/ CD63/ Flotillin-1/ HSP70/ GFP
non-EV: GRP94 Proteomics
no
Show all info
Study aim
Mechanism of uptake/transfer/New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HEK293-GFP
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
SW 40 Ti
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ Flotillin-1/ HSP70/ GFP
Not detected contaminants
GRP94
Flow cytometry
Type of Flow cytometry
Beckman Coulter Cytoflex
Hardware adaptation to ~100nm EV's
The better resolution of the CytoFLEX is reached by using the violet side scatter of the 405 nm laser (manually set to 1600 and height threshold) and by performing preanalytical preparations with Fluorescent Megamix-Plus SSC beads (Cosmo Bio Co., LTD, Japan) which are FITC-labeled beads of increasing size (100, 160, 200, 240, 300, 500, 900 nm). beads were used to set the EV gate and manual gating was set to the populations of interest with reference to a negative control sample (GFP- EVs from HEK293 cells)
Calibration bead size
0.1/ 0.16/ 0.2/ 0.24/ 0.3/ 0.5/ 0.9
Antibody details provided?
No
Detected EV-associated proteins
GFP
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
~172
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 6.00E+09
Particle analysis: flow cytometry
Flow cytometer type
Nanoscale
Hardware adjustment
The better resolution of the CytoFLEX is reached by using the violet side scatter of the 405 nm laser (manually set to 1600 and height threshold) and by performing preanalytical preparations with Fluorescent Megamix-Plus SSC beads (Cosmo Bio Co., LTD, Japan) which are FITC-labeled beads of increasing size (100, 160, 200, 240, 300, 500, 900 nm). beads were used to set the EV gate and manual gating was set to the populations of interest with reference to a negative control sample (GFP- EVs from HEK293 cells)
Calibration bead size
0.1/ 0.16/ 0.2/ 0.24/ 0.3/ 0.5/ 0.9
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 9.00E+06
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV240036 | 5/6 | Homo sapiens | Serum |
Exoquick IAF |
Shinde U | 2024 | 63% | |
Study summaryFull title
All authors
Shinde U, Rao A, Bansal V, Das DK, Balasinor NH, Madan T
Journal
Reproduction
Abstract
Circulating extracellular vesicles of placental/amniochorionic origin carry placental/amniochorionic (show more...)
EV-METRIC
63% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Pregnant
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Commercial method
Immunoaffinity capture (non-commercial) Protein markers
EV: CD9/ CD63/ PLAP/ Cullin 7
non-EV: Calnexin Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Commercial kit
Exoquick
Immunoaffinity capture
Selected surface protein(s)
PLAP
EV-subtype
Distinction between multiple subtypes
affinity capture
Used subtypes
PLAP+
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ PLAP/ Cullin 7
Not detected contaminants
Calnexin
Characterization: RNA analysis
RNA analysis
Type
RT(q)PCR
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
RNAse
RNAse concentration
20mg/mL
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
30-150 nm
NTA
Report type
Modus
Reported size (nm)
30-150 nm
EV concentration
Yes
Particle yield
number of particles per million cells: 1E10 particles/ml
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
30-150 nm
|
||||||||
EV230572 | 4/6 | Homo sapiens | HEK293-GFP | PEI precipitation | Djeungoue-Petga, Marie-Ange | 2024 | 63% | |
Study summaryFull title
All authors
Marie Ange Djeungoue Petgaa, Catherine Taylora, Alexander Macpherson, Surendar Reddy Dhadi, Thomas Rollin, Jeremy W. Roya, Anirban Ghosh, Stephen M. Lewis, Rodney J. Ouellette
Journal
Abstract
Extracellular vesicles (EVs) are gaining interest as efficient, biocompatible vehicles for cellular (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
GFP overexpresion
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
PEI precipitation
Protein markers
EV: CD9/ CD63/ Flotillin-1/ HSP70/ GFP
non-EV: GRP94 Proteomics
no
Show all info
Study aim
Mechanism of uptake/transfer/New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HEK293-GFP
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
Other
Name other separation method
PEI precipitation
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ Flotillin-1/ HSP70/ GFP
Not detected contaminants
GRP94
Flow cytometry
Type of Flow cytometry
Beckman Coulter Cytoflex
Hardware adaptation to ~100nm EV's
The better resolution of the CytoFLEX is reached by using the violet side scatter of the 405 nm laser (manually set to 1600 and height threshold) and by performing preanalytical preparations with Fluorescent Megamix-Plus SSC beads (Cosmo Bio Co., LTD, Japan) which are FITC-labeled beads of increasing size (100, 160, 200, 240, 300, 500, 900 nm). beads were used to set the EV gate and manual gating was set to the populations of interest with reference to a negative control sample (GFP- EVs from HEK293 cells)
Calibration bead size
0.1/ 0.16/ 0.2/ 0.24/ 0.3/ 0.5/ 0.9
Antibody details provided?
No
Detected EV-associated proteins
GFP
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
146-200
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1-6E10
Particle analysis: flow cytometry
Flow cytometer type
Nanoscale
Hardware adjustment
The better resolution of the CytoFLEX is reached by using the violet side scatter of the 405 nm laser (manually set to 1600 and height threshold) and by performing preanalytical preparations with Fluorescent Megamix-Plus SSC beads (Cosmo Bio Co., LTD, Japan) which are FITC-labeled beads of increasing size (100, 160, 200, 240, 300, 500, 900 nm). beads were used to set the EV gate and manual gating was set to the populations of interest with reference to a negative control sample (GFP- EVs from HEK293 cells)
Calibration bead size
0.1/ 0.16/ 0.2/ 0.24/ 0.3/ 0.5/ 0.9
Particle yield
particles per milliliter of starting sample: 1-6E10
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230012 | 1/4 | Mus musculus | cardiac mesenchymal stromal cells |
Filtration UF SEC (non-commercial) |
Caller T | 2024 | 63% | |
Study summaryFull title
All authors
Caller T, Rotem I, Shaihov-Teper O, Lendengolts D, Schary Y, Shai R, Glick-Saar E, Dominissini D, Motiei M, Katzir I, Popovtzer R, Nahmoud M, Boomgarden A, D'Souza-Schorey C, Naftali-Shani N, Leor J
Journal
Circulation
Abstract
Myocardial infarction (MI) and heart failure are associated with an increased incidence of cancer. H (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
Ultrafiltration Size-exclusion chromatography (non-commercial) Protein markers
EV: CD9/ CD81/ TSG101
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
cardiac mesenchymal stromal cells
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
3000000
Separation Method
Filtration steps
0.8
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Cellulose acetate
Size-exclusion chromatography
Total column volume (mL)
70
Sample volume/column (mL)
10
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD81/ TSG101
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
>250
EV concentration
Yes
Particle yield
number of particles per million cells: 90000000
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV230012 | 2/4 | Mus musculus | cardiac mesenchymal stromal cells |
Filtration UF SEC (non-commercial) |
Caller T | 2024 | 63% | |
Study summaryFull title
All authors
Caller T, Rotem I, Shaihov-Teper O, Lendengolts D, Schary Y, Shai R, Glick-Saar E, Dominissini D, Motiei M, Katzir I, Popovtzer R, Nahmoud M, Boomgarden A, D'Souza-Schorey C, Naftali-Shani N, Leor J
Journal
Circulation
Abstract
Myocardial infarction (MI) and heart failure are associated with an increased incidence of cancer. H (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Heart failure
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
Ultrafiltration Size-exclusion chromatography (non-commercial) Protein markers
EV: CD9/ CD81/ TSG101
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
cardiac mesenchymal stromal cells
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
3000000
Separation Method
Filtration steps
0.8
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Cellulose acetate
Size-exclusion chromatography
Total column volume (mL)
70
Sample volume/column (mL)
10
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD81/ TSG101
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
<250
EV concentration
Yes
Particle yield
number of particles per million cells: 40000000
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV230012 | 3/4 | Mus musculus | Cardiac tissue |
Filtration UF SEC (non-commercial) |
Caller T | 2024 | 63% | |
Study summaryFull title
All authors
Caller T, Rotem I, Shaihov-Teper O, Lendengolts D, Schary Y, Shai R, Glick-Saar E, Dominissini D, Motiei M, Katzir I, Popovtzer R, Nahmoud M, Boomgarden A, D'Souza-Schorey C, Naftali-Shani N, Leor J
Journal
Circulation
Abstract
Myocardial infarction (MI) and heart failure are associated with an increased incidence of cancer. H (show more...)
EV-METRIC
63% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cardiac tissue
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
Ultrafiltration Size-exclusion chromatography (non-commercial) Protein markers
EV: CD9/ CD81/ TSG101
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cardiac tissue
Separation Method
Filtration steps
0.8
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Cellulose acetate
Size-exclusion chromatography
Total column volume (mL)
70
Sample volume/column (mL)
10
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD81/ TSG101
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
<250
EV concentration
Yes
Particle yield
number of particles per 100mg tissue: 90000000
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230012 | 4/4 | Mus musculus | Cardiac tissue |
Filtration UF SEC (non-commercial) |
Caller T | 2024 | 63% | |
Study summaryFull title
All authors
Caller T, Rotem I, Shaihov-Teper O, Lendengolts D, Schary Y, Shai R, Glick-Saar E, Dominissini D, Motiei M, Katzir I, Popovtzer R, Nahmoud M, Boomgarden A, D'Souza-Schorey C, Naftali-Shani N, Leor J
Journal
Circulation
Abstract
Myocardial infarction (MI) and heart failure are associated with an increased incidence of cancer. H (show more...)
EV-METRIC
63% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cardiac tissue
Sample origin
Heart failure
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
Ultrafiltration Size-exclusion chromatography (non-commercial) Protein markers
EV: CD9/ CD81/ TSG101
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cardiac tissue
Separation Method
Filtration steps
0.8
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Cellulose acetate
Size-exclusion chromatography
Total column volume (mL)
70
Sample volume/column (mL)
10
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD81/ TSG101
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
<250
EV concentration
Yes
Particle yield
number of particles per 100 mg tissue: 190000000
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV210336 | 1/2 | Homo sapiens | THP1 |
(d)(U)C DG |
Driedonks, Tom | 2024 | 63% | |
Study summaryFull title
All authors
Tom A. P. Driedonks, Sarah Ressel, Thi Tran Ngoc Minh, Amy H. Buck, Esther N. M. Nolte-‘t Hoen
Journal
J Extracell Biol
Abstract
Cells can communicate via the release and uptake of extracellular vesicles (EVs), which are nano-siz (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: CD9/ CD63/ CD81
non-EV: None Proteomics
no
EV density (g/ml)
1.11 -1.18
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
THP1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Cell viability (%)
95
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Density gradient
Type
Continuous
Lowest density fraction
0.4 M
Highest density fraction
2.0 M
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
0.2
Orientation
Bottom-up
Speed (g)
192000
Duration (min)
900-1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
4
Pelleting: speed (g)
192000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR/ Capillary electrophoresis (e.g. Bioanalyzer)
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
BD Influx
Hardware adjustment
according to van der Vlist et al, Nature Protocols 2012
Calibration bead size
0.1/ 0.2
EV concentration
Yes
|
||||||||
EV210336 | 2/2 | Homo sapiens | THP1 |
(d)(U)C DG |
Driedonks, Tom | 2024 | 63% | |
Study summaryFull title
All authors
Tom A. P. Driedonks, Sarah Ressel, Thi Tran Ngoc Minh, Amy H. Buck, Esther N. M. Nolte-‘t Hoen
Journal
J Extracell Biol
Abstract
Cells can communicate via the release and uptake of extracellular vesicles (EVs), which are nano-siz (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Pam3CSK4 treated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: CD9/ CD63/ CD81
non-EV: None Proteomics
no
EV density (g/ml)
1.11 -1.18
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
THP1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Cell viability (%)
95
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Density gradient
Type
Continuous
Lowest density fraction
0.4 M
Highest density fraction
2.0 M
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
0.2
Orientation
Bottom-up
Speed (g)
192000
Duration (min)
900-1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
4
Pelleting: speed (g)
192000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR/ Capillary electrophoresis (e.g. Bioanalyzer)
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
BD Influx
Hardware adjustment
according to van der Vlist et al Nature Protocols 2012
Calibration bead size
0.1/ 0.2
EV concentration
Yes
|
||||||||
EV230597 | 3/9 | Homo sapiens | 293T | (d)(U)C | Chen Z | 2024 | 56% | |
Study summaryFull title
All authors
Chen Z, Luo L, Ye T, Zhou J, Niu X, Yuan J, Yuan T, Fu D, Li H, Li Q, Wang Y
Journal
J Extracell Vesicles
Abstract
Pluripotent stem cell-derived small extracellular vesicles (PSC-sEVs) have demonstrated great clinic (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ CD9/ CD63/ TSG101/ CD81
non-EV: GM130/ Calnexin Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
293T
EV-harvesting Medium
Serum free medium
Cell count
1.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
25
Wash: time (min)
70
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ CD63/ TSG101
Not detected contaminants
GM130/ Calnexin
Flow cytometry
Type of Flow cytometry
Nano-flow Cytometry
Hardware adaptation to ~100nm EV's
By utilizing Rayleigh scattering
Calibration bead size
0.25
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
Nano-flow Cytometry
Hardware adjustment
By utilizing Rayleigh scattering
Calibration bead size
68/ 91,113,155
Report type
Size range/distribution
Reported size (nm)
40-150
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.20E+08
|
||||||||
EV230597 | 4/9 | Homo sapiens | huMSC | (d)(U)C | Chen Z | 2024 | 56% | |
Study summaryFull title
All authors
Chen Z, Luo L, Ye T, Zhou J, Niu X, Yuan J, Yuan T, Fu D, Li H, Li Q, Wang Y
Journal
J Extracell Vesicles
Abstract
Pluripotent stem cell-derived small extracellular vesicles (PSC-sEVs) have demonstrated great clinic (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD9/ CD63/ TSG101/ CD81
non-EV: GM130/ Calnexin Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
huMSC
EV-harvesting Medium
Serum free medium
Cell count
5.00E+06
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
25
Wash: time (min)
70
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ TSG101
Not detected contaminants
GM130/ Calnexin
Flow cytometry
Type of Flow cytometry
Nano-flow Cytometry
Hardware adaptation to ~100nm EV's
By utilizing Rayleigh scattering
Calibration bead size
0.25
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
Nano-flow Cytometry
Hardware adjustment
By utilizing Rayleigh scattering
Calibration bead size
68/ 91/ 113/ 155
Report type
Size range/distribution
Reported size (nm)
40-150
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 7.71E+07
|
||||||||
EV230597 | 5/9 | Homo sapiens | HFF-1 | (d)(U)C | Chen Z | 2024 | 56% | |
Study summaryFull title
All authors
Chen Z, Luo L, Ye T, Zhou J, Niu X, Yuan J, Yuan T, Fu D, Li H, Li Q, Wang Y
Journal
J Extracell Vesicles
Abstract
Pluripotent stem cell-derived small extracellular vesicles (PSC-sEVs) have demonstrated great clinic (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ CD9/ CD63/ TSG101/ CD81
non-EV: GM130/ Calnexin Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HFF-1
EV-harvesting Medium
Serum free medium
Cell count
1.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
25
Wash: time (min)
70
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ CD63/ TSG101
Not detected contaminants
GM130/ Calnexin
Flow cytometry
Type of Flow cytometry
Nano-flow Cytometry
Hardware adaptation to ~100nm EV's
By utilizing Rayleigh scattering
Calibration bead size
0.25
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
Nano-flow Cytometry
Hardware adjustment
By utilizing Rayleigh scattering
Calibration bead size
68/ 91/ 113/ 155
Report type
Size range/distribution
Reported size (nm)
40-150
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 8.57E+07
|
||||||||
EV230597 | 6/9 | Homo sapiens | A549 | (d)(U)C | Chen Z | 2024 | 56% | |
Study summaryFull title
All authors
Chen Z, Luo L, Ye T, Zhou J, Niu X, Yuan J, Yuan T, Fu D, Li H, Li Q, Wang Y
Journal
J Extracell Vesicles
Abstract
Pluripotent stem cell-derived small extracellular vesicles (PSC-sEVs) have demonstrated great clinic (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ CD9/ CD63/ TSG101/ CD81
non-EV: GM130/ Calnexin Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
A549
EV-harvesting Medium
Serum free medium
Cell count
1.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
25
Wash: time (min)
70
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ CD63/ TSG101
Not detected contaminants
GM130/ Calnexin
Flow cytometry
Type of Flow cytometry
Nano-flow Cytometry
Hardware adaptation to ~100nm EV's
By utilizing Rayleigh scattering
Calibration bead size
0.25
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
Nano-flow Cytometry
Hardware adjustment
By utilizing Rayleigh scattering
Calibration bead size
68/ 91/ 113/ 155
Report type
Size range/distribution
Reported size (nm)
40-150
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 9.78E+07
|
||||||||
EV230597 | 7/9 | Homo sapiens | H460 | (d)(U)C | Chen Z | 2024 | 56% | |
Study summaryFull title
All authors
Chen Z, Luo L, Ye T, Zhou J, Niu X, Yuan J, Yuan T, Fu D, Li H, Li Q, Wang Y
Journal
J Extracell Vesicles
Abstract
Pluripotent stem cell-derived small extracellular vesicles (PSC-sEVs) have demonstrated great clinic (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ CD9/ CD63/ TSG101/ CD81
non-EV: GM130/ Calnexin Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
H460
EV-harvesting Medium
Serum free medium
Cell count
1.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
25
Wash: time (min)
70
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ CD63/ TSG101
Not detected contaminants
GM130/ Calnexin
Flow cytometry
Type of Flow cytometry
Nano-flow Cytometry
Hardware adaptation to ~100nm EV's
By utilizing Rayleigh scattering
Calibration bead size
0.25
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
Nano-flow Cytometry
Hardware adjustment
By utilizing Rayleigh scattering
Calibration bead size
68/ 91/ 113/ 155
Report type
Size range/distribution
Reported size (nm)
40-150
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.44E+08
|
||||||||
EV230597 | 8/9 | Homo sapiens | MCF7 | (d)(U)C | Chen Z | 2024 | 56% | |
Study summaryFull title
All authors
Chen Z, Luo L, Ye T, Zhou J, Niu X, Yuan J, Yuan T, Fu D, Li H, Li Q, Wang Y
Journal
J Extracell Vesicles
Abstract
Pluripotent stem cell-derived small extracellular vesicles (PSC-sEVs) have demonstrated great clinic (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ CD9/ CD63/ TSG101/ CD81
non-EV: GM130/ Calnexin Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MCF7
EV-harvesting Medium
Serum free medium
Cell count
1.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
25
Wash: time (min)
70
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ CD63/ TSG101
Not detected contaminants
GM130/ Calnexin
Flow cytometry
Type of Flow cytometry
Nano-flow Cytometry
Hardware adaptation to ~100nm EV's
By utilizing Rayleigh scattering
Calibration bead size
0.25
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
Nano-flow Cytometry
Hardware adjustment
By utilizing Rayleigh scattering
Calibration bead size
68/ 91/ 113/ 155
Report type
Size range/distribution
Reported size (nm)
40-150
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.86E+08
|
||||||||
EV230597 | 9/9 | Homo sapiens | MDAMB231 | (d)(U)C | Chen Z | 2024 | 56% | |
Study summaryFull title
All authors
Chen Z, Luo L, Ye T, Zhou J, Niu X, Yuan J, Yuan T, Fu D, Li H, Li Q, Wang Y
Journal
J Extracell Vesicles
Abstract
Pluripotent stem cell-derived small extracellular vesicles (PSC-sEVs) have demonstrated great clinic (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ CD9/ CD63/ TSG101/ CD81
non-EV: GM130/ Calnexin Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MDAMB231
EV-harvesting Medium
Serum free medium
Cell count
1.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
25
Wash: time (min)
70
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ CD63/ TSG101
Not detected contaminants
GM130/ Calnexin
Flow cytometry
Type of Flow cytometry
Nano-flow Cytometry
Hardware adaptation to ~100nm EV's
By utilizing Rayleigh scattering
Calibration bead size
0.25
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
Nano-flow Cytometry
Hardware adjustment
By utilizing Rayleigh scattering
Calibration bead size
68/ 91/ 113/ 155
Report type
Size range/distribution
Reported size (nm)
40-150
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.68E+08
|
||||||||
EV230572 | 1/6 | Homo sapiens | HEK293 | (d)(U)C | Djeungoue-Petga, Marie-Ange | 2024 | 56% | |
Study summaryFull title
All authors
Marie Ange Djeungoue Petgaa, Catherine Taylora, Alexander Macpherson, Surendar Reddy Dhadi, Thomas Rollin, Jeremy W. Roya, Anirban Ghosh, Stephen M. Lewis, Rodney J. Ouellette
Journal
Abstract
Extracellular vesicles (EVs) are gaining interest as efficient, biocompatible vehicles for cellular (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD9/ CD63/ Flotillin-1/ HSP70/ GFP
non-EV: GRP94 Proteomics
no
Show all info
Study aim
Mechanism of uptake/transfer/New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HEK293
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
SW 40 Ti
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ Flotillin-1/ HSP70
Not detected EV-associated proteins
GFP
Not detected contaminants
GRP94
Flow cytometry
Type of Flow cytometry
Beckman Coulter Cytoflex
Hardware adaptation to ~100nm EV's
The better resolution of the CytoFLEX is reached by using the violet side scatter of the 405 nm laser (manually set to 1600 and height threshold) and by performing preanalytical preparations with Fluorescent Megamix-Plus SSC beads (Cosmo Bio Co., LTD, Japan) which are FITC-labeled beads of increasing size (100, 160, 200, 240, 300, 500, 900 nm). beads were used to set the EV gate and manual gating was set to the populations of interest with reference to a negative control sample (GFP- EVs from HEK293 cells)
Calibration bead size
0.1/ 0.16/ 0.2/ 0.24/ 0.3/ 0.5/ 0.9
Antibody details provided?
No
Not detected EV-associated proteins
GFP
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
Extra information
two kinds of PEI were used. Linear PEI 25 kDa and Branched 10 kDa
|
||||||||
EV230372 | 1/14 | Homo sapiens | A549 | (d)(U)C | Schöne N | 2024 | 56% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
large EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Actinin-4/ CD81/ Rgap1/ Syntenin/ EMMPRIN/ EpCAM/ EGFR/ Mitofilin/ Arf6/ HDAC1
non-EV: GM130/ HDAC1/ Albumin/ ApoA1/ ApoB Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
A549
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
17000
Wash: volume per pellet (ml)
1
Wash: time (min)
30
Wash: Rotor Type
Heraeus 3331
Wash: speed (g)
17000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Actinin-4/ Mitofilin/ Arf6
Not detected EV-associated proteins
CD81/ Rgap1/ Syntenin
Detected contaminants
ApoB
Not detected contaminants
GM130/ HDAC1/ Albumin/ ApoA1
Flow cytometry
Type of Flow cytometry
standard flow cytometer
Calibration bead size
0.2, 0.8
Antibody details provided?
No
Detected EV-associated proteins
EMMPRIN/ EpCAM/ EGFR
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
163.6
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 8.71E+08
|
||||||||
EV230372 | 2/14 | Homo sapiens | A549 | (d)(U)C | Schöne N | 2024 | 56% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Syntenin/ Actinin-4/ Arf6/ Rgap1/ Mitofilin
non-EV: GM130/ HDAC1/ Albumin/ ApoA1/ ApoB Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
A549
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
143000
Wash: volume per pellet (ml)
1.4
Wash: time (min)
90
Wash: Rotor Type
TLA-55
Wash: speed (g)
143000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Syntenin/ Actinin-4/ Arf6
Not detected EV-associated proteins
Rgap1/ Mitofilin
Not detected contaminants
GM130/ HDAC1/ Albumin/ ApoA1/ ApoB
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
147.13
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.95E+09
|
||||||||
EV230372 | 4/14 | Homo sapiens | HCC-78 | (d)(U)C | Schöne N | 2024 | 56% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Syntenin/ Actinin-4/ Arf6/ Rgap1/ Mitofilin
non-EV: GM130/ HDAC1/ Albumin/ ApoA1/ ApoB Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HCC-78
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
143000
Wash: volume per pellet (ml)
1.4
Wash: time (min)
90
Wash: Rotor Type
TLA-55
Wash: speed (g)
143000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Syntenin/ Arf6
Not detected EV-associated proteins
Rgap1/ Actinin-4/ Mitofilin
Not detected contaminants
GM130/ HDAC1/ Albumin/ ApoA1/ ApoB
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
163.6
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 2.26E+10
|
||||||||
EV230372 | 5/14 | Homo sapiens | H1975 | (d)(U)C | Schöne N | 2024 | 56% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
large EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Actinin-4/ Rgap1/ Syntenin-1/ CD81/ EMMPRIN/ EpCAM/ EGFR/ Mitofilin,Arf6
non-EV: GM130/ HDAC1/ Albumin/ ApoA1/ ApoB Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
H1975
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
17000
Wash: volume per pellet (ml)
1
Wash: time (min)
30
Wash: Rotor Type
Heraeus 3331
Wash: speed (g)
17000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Actinin-4/ Rgap1/ Mitofilin/ Arf6
Not detected EV-associated proteins
CD81/ Syntenin
Detected contaminants
GM130
Not detected contaminants
HDAC1/ Albumin/ ApoA1/ ApoB
Flow cytometry
Type of Flow cytometry
standard flow cytometer
Calibration bead size
0.2, 0.8
Antibody details provided?
No
Detected EV-associated proteins
EMMPRIN/ EpCAM/ EGFR
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
180.6
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.37E+10
|
||||||||
EV230372 | 6/14 | Homo sapiens | H1975 | (d)(U)C | Schöne N | 2024 | 56% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Syntenin/ Actinin-4/ Arf6/ Rgap1/ Mitofilin
non-EV: GM130/ HDAC1/ Albumin/ ApoA1/ ApoB Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
H1975
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
143000
Wash: volume per pellet (ml)
1.4
Wash: time (min)
90
Wash: Rotor Type
TLA-55
Wash: speed (g)
143000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ Syntenin/ Actinin-4
Not detected EV-associated proteins
Rgap1/ Mitofilin/ Arf6
Not detected contaminants
GM130/ HDAC1/ Albumin/ ApoA1/ ApoB
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
163.9
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.43E+10
|
||||||||
EV230372 | 11/14 | Homo sapiens | Blood plasma | (d)(U)C | Schöne N | 2024 | 56% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
56% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
large EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Actinin-4/ Rgap1/ EMMPRIN/ EpCAM
non-EV: ApoA1/ ApoB Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
17000
Wash: volume per pellet (ml)
1
Wash: time (min)
30
Wash: Rotor Type
Heraeus 3331
Wash: speed (g)
17000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Actinin-4/ Rgap1/ EMMPRIN
Detected contaminants
ApoB
Not detected contaminants
ApoA1/ ApoA1
Flow cytometry
Type of Flow cytometry
standard flow cytometer
Calibration bead size
0.2, 0.8
Antibody details provided?
No
Detected EV-associated proteins
EMMPRIN/ EpCAM
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
185.2
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 5.01E+08
|
||||||||
EV220329 | 1/2 | Homo sapiens | Blood plasma | (d)(U)C | Chong MC | 2024 | 56% | |
Study summaryFull title
All authors
Chong MC, Shah AD, Schittenhelm RB, Silva A, James PF, Wu SSX, Howitt J
Journal
Acta Physiol (Oxf)
Abstract
Physical exercise triggers the secretion of small extracellular vesicles (sEVs) into the circulation (show more...)
EV-METRIC
56% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: TSG101/ Syntenin-1
non-EV: ApoA-1/ GM130 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100,000
Wash: volume per pellet (ml)
20
Wash: time (min)
60
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
100,000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TSG101/ Syntenin-1
Detected contaminants
ApoA-1
Not detected contaminants
GM130
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
Other particle analysis name(1)
Microfluidic resistive pulse sensing
Report type
Mean
Report size
88
EV-concentration
Yes
Particle yield
as number of particles per milliliter of starting sample: 2.80E+10
|
||||||||
EV220329 | 2/2 | Homo sapiens | Blood plasma | (d)(U)C | Chong MC | 2024 | 56% | |
Study summaryFull title
All authors
Chong MC, Shah AD, Schittenhelm RB, Silva A, James PF, Wu SSX, Howitt J
Journal
Acta Physiol (Oxf)
Abstract
Physical exercise triggers the secretion of small extracellular vesicles (sEVs) into the circulation (show more...)
EV-METRIC
56% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
post-exercise
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: TSG101/ Syntenin-1
non-EV: ApoA-1/ GM130 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100,000
Wash: volume per pellet (ml)
20
Wash: time (min)
60
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
100,000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TSG101/ Syntenin-1
Detected contaminants
ApoA-1
Not detected contaminants
GM130
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
Other particle analysis name(1)
Microfluidic resistive pulse sensing
Report type
Mean
Report size
89
EV-concentration
Yes
Particle yield
as number of particles per milliliter of starting sample: 3.90E+10
|
||||||||
EV240036 | 2/6 | Homo sapiens | Serum |
Exoquick IAF |
Shinde U | 2024 | 50% | |
Study summaryFull title
All authors
Shinde U, Rao A, Bansal V, Das DK, Balasinor NH, Madan T
Journal
Reproduction
Abstract
Circulating extracellular vesicles of placental/amniochorionic origin carry placental/amniochorionic (show more...)
EV-METRIC
50% (89th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Non pregnant
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Commercial method
Immunoaffinity capture (non-commercial) Protein markers
EV: CD9/ CD63/ PLAP/ Cullin 7
non-EV: Calnexin Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Commercial kit
Exoquick
Immunoaffinity capture
Selected surface protein(s)
PLAP
EV-subtype
Distinction between multiple subtypes
affinity capture
Used subtypes
PLAP+
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ PLAP/ Cullin 7
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
30-150 nm
EV concentration
Yes
Particle yield
number of particles per million cells: 1E10 particles/ml
|
||||||||
EV240036 | 4/6 | Homo sapiens | Serum | Exoquick | Shinde U | 2024 | 50% | |
Study summaryFull title
All authors
Shinde U, Rao A, Bansal V, Das DK, Balasinor NH, Madan T
Journal
Reproduction
Abstract
Circulating extracellular vesicles of placental/amniochorionic origin carry placental/amniochorionic (show more...)
EV-METRIC
50% (89th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Pregnant
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Commercial method
Protein markers
EV: CD9/ CD63/ PLAP/ Cullin 7
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Commercial kit
Exoquick
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ PLAP/ Cullin 7
Characterization: RNA analysis
RNA analysis
Type
RT(q)PCR
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
RNAse
RNAse concentration
20mg/mL
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
30-150 nm
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
30-150 nm
|
||||||||
EV240036 | 6/6 | Homo sapiens | Serum |
Exoquick IAF |
Shinde U | 2024 | 50% | |
Study summaryFull title
All authors
Shinde U, Rao A, Bansal V, Das DK, Balasinor NH, Madan T
Journal
Reproduction
Abstract
Circulating extracellular vesicles of placental/amniochorionic origin carry placental/amniochorionic (show more...)
EV-METRIC
50% (89th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Pregnant
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Commercial method
Immunoaffinity capture (non-commercial) Protein markers
EV: PLAP/ HLA-G
non-EV: Calnexin Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Commercial kit
Exoquick
Immunoaffinity capture
Selected surface protein(s)
PLAP/ HLA-G
EV-subtype
Distinction between multiple subtypes
affinity capture
Used subtypes
PLAP+ HLA-G+
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
PLAP/ HLA-G
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
30-150 nm
EV concentration
Yes
Particle yield
number of particles per million cells: 1E10 particles/ml
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
30-150 nm
|
||||||||
EV240006 | 3/4 | Rattus norvegicus | INS-1 |
(d)(U)C Total Exosome Isolation lipid-based affinity capture |
Weerakkody, Jonathan S. | 2024 | 50% | |
Study summaryFull title
All authors
Jonathan S. Weerakkody, Tiffany Tseng, Mackenzie Topper, Sikha Thoduvayil, Abhijith Radhakrishnan, Frederic Pincet, Themis R. Kyriakides, Roshan W. Gunasekara, Sathish Ramakrishnan
Journal
Abstract
The biggest challenge in current isolation methods for lipid bilayer-encapsulated vesicles, such as (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small extracellular vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Total Exosome Isolation lipid-based affinity capture Protein markers
EV: CD9/ CD81
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Rattus norvegicus
Sample Type
Cell culture supernatant
EV-producing cells
INS-1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: rotor type
SW 55 Ti
Pelleting: speed (g)
12500
Commercial kit
Total Exosome Isolation
Other
Name other separation method
Total Exosome Isolation
Other
Name other separation method
lipid-based affinity capture
EV-subtype
Distinction between multiple subtypes
Size
Used subtypes
30-200
Characterization: Protein analysis
Protein Concentration Method
Not determined
Detected EV-associated proteins
CD9/ CD81
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
104
NTA
Report type
Mean
Reported size (nm)
126
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.50E+07
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Other particle analysis name(1)
dSTORM single molecule localization microscopy
Report type
Mean
Report size
89
EV-concentration
No
Extra information
This paper was to validate the efficacy of photosensitive lipid nanoprobe for the isolation and size selective enrichment of native extracellular vesicles
|
||||||||
1 - 50 of 70 | keyboard_arrow_right |