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You searched for: 2024 (Year of publication)
Showing 1 - 50 of 143
Showing 1 - 50 of 143
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV230597 | 1/9 | Homo sapiens | ESC |
(d)(U)C DG |
Chen Z | 2024 | 100% | |
Study summaryFull title
All authors
Chen Z, Luo L, Ye T, Zhou J, Niu X, Yuan J, Yuan T, Fu D, Li H, Li Q, Wang Y
Journal
J Extracell Vesicles
Abstract
Pluripotent stem cell-derived small extracellular vesicles (PSC-sEVs) have demonstrated great clinic (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Adj. k-factor
37432 (washing)
Protein markers
EV: Alix/ CD9/ CD63/ TSG101/ CD81
non-EV: GM130/ Calnexin Proteomics
yes
EV density (g/ml)
1.17
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
ESC
EV-harvesting Medium
Serum free medium
Cell count
2.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
25
Wash: time (min)
70
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
100000
Wash: adjusted k-factor
37432
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
20%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
11
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
150000
Duration (min)
600
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
10
Pelleting: speed (g)
150000
Pelleting: adjusted k-factor
10018
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
Alix/ CD9/ CD63/ TSG101
Not detected contaminants
GM130/ Calnexin
Flow cytometry
Type of Flow cytometry
Nano-flow Cytometry
Hardware adaptation to ~100nm EV's
By utilizing Rayleigh scattering
Calibration bead size
0.25
Detected EV-associated proteins
CD9/ CD63/ CD81
Proteomics database
ProteomeXchange
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
Nano-flow Cytometry
Hardware adjustment
By utilizing Rayleigh scattering
Calibration bead size
68/ 91,113,155
Report type
Size range/distribution
Reported size (nm)
40-150
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 2.03E+08
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
100
|
||||||||
EV230597 | 2/9 | Homo sapiens | iPSC |
(d)(U)C DG |
Chen Z | 2024 | 100% | |
Study summaryFull title
All authors
Chen Z, Luo L, Ye T, Zhou J, Niu X, Yuan J, Yuan T, Fu D, Li H, Li Q, Wang Y
Journal
J Extracell Vesicles
Abstract
Pluripotent stem cell-derived small extracellular vesicles (PSC-sEVs) have demonstrated great clinic (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Adj. k-factor
37432 (washing)
Protein markers
EV: TSG101/ CD9/ CD63/ CD81
non-EV: None Proteomics
yes
EV density (g/ml)
1.17
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
iPSC
EV-harvesting Medium
Serum free medium
Cell count
2.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
25
Wash: time (min)
70
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
100000
Wash: adjusted k-factor
37432
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
20%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
11
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
150000
Duration (min)
600
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
10
Pelleting: speed (g)
150000
Pelleting: adjusted k-factor
10018
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
Alix/ CD9/ CD63/ TSG101
Not detected contaminants
GM130/ Calnexin
Flow cytometry
Type of Flow cytometry
Nano-flow Cytometry
Hardware adaptation to ~100nm EV's
By utilizing Rayleigh scattering
Calibration bead size
0.25
Detected EV-associated proteins
CD9/ CD63/ CD81
Proteomics database
ProteomeXchange
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
Nano-flow Cytometry
Hardware adjustment
By utilizing Rayleigh scattering
Calibration bead size
68/ 91,113,155
Report type
Size range/distribution
Reported size (nm)
40-150
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 2.03E+08
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
100
|
||||||||
EV230372 | 7/14 | Homo sapiens | H2228 |
(d)(U)C DG |
Schöne N | 2024 | 100% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
large EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: Actinin-4/ Rgap1/ Syntenin-1/ CD81/ EMMPRIN/ EpCAM/ EGFR/ Mitofilin,Arf6
non-EV: GM130/ HDAC1/ Albumin/ ApoA1/ ApoB Proteomics
no
EV density (g/ml)
1.10-1.17
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
H2228
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
17000
Wash: volume per pellet (ml)
1
Wash: time (min)
30
Wash: Rotor Type
Heraeus 3331
Wash: speed (g)
17000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
16.5
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
16
Pelleting: speed (g)
100000
Pelleting-wash: volume per pellet (mL)
16
Pelleting-wash: duration (min)
60
Pelleting-wash: speed (g)
SW 32 Ti
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
Actinin-4/ Rgap1/ Mitofilin/ Arf6/ CD81
Not detected EV-associated proteins
Syntenin
Not detected contaminants
GM130/ HDAC1/ Albumin/ ApoA1/ ApoB
Flow cytometry
Type of Flow cytometry
standard flow cytometer
Calibration bead size
0.2, 0.8
Detected EV-associated proteins
EMMPRIN/ EpCAM/ EGFR
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
196.8
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 4.83E+09
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230372 | 9/14 | Homo sapiens | H596 |
(d)(U)C DG |
Schöne N | 2024 | 100% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
large EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: Actinin-4/ Rgap1/ Syntenin-1/ CD81/ EMMPRIN/ EpCAM/ EGFR/ Mitofilin,Arf6
non-EV: GM130/ HDAC1/ Albumin/ ApoA1/ ApoB Proteomics
no
EV density (g/ml)
1.10-1.17
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
H596
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
17000
Wash: volume per pellet (ml)
1
Wash: time (min)
30
Wash: Rotor Type
Heraeus 3331
Wash: speed (g)
17000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
16.5
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
16
Pelleting: speed (g)
100000
Pelleting-wash: volume per pellet (mL)
16
Pelleting-wash: duration (min)
60
Pelleting-wash: speed (g)
SW 32 Ti
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
Actinin-4/ Rgap1/ Mitofilin/ Arf6
Not detected EV-associated proteins
Syntenin/ CD81
Not detected contaminants
GM130/ HDAC1/ Albumin/ ApoA1/ ApoB
Flow cytometry
Type of Flow cytometry
standard flow cytometer
Calibration bead size
0.2, 0.8
Detected EV-associated proteins
EMMPRIN/ EpCAM/ EGFR
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
188.7
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.33E+09
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230372 | 13/14 | Homo sapiens | Blood plasma | (d)(U)C | Schöne N | 2024 | 100% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NSCLC
Focus vesicles
large EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Actinin-4/ Rgap1/ EMMPRIN/ EpCAM/ EGFR
non-EV: ApoA1/ ApoB Proteomics
no
EV density (g/ml)
1.10-1.17
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
17000
Wash: volume per pellet (ml)
1
Wash: time (min)
30
Wash: Rotor Type
Heraeus 3331
Wash: speed (g)
17000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
16.5
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
16
Pelleting: speed (g)
100000
Pelleting-wash: volume per pellet (mL)
16
Pelleting-wash: duration (min)
60
Pelleting-wash: speed (g)
SW 32 Ti
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
Actinin-4/ Rgap1/ EMMPRIN
Detected contaminants
ApoB
Not detected contaminants
ApoA1
Flow cytometry
Type of Flow cytometry
standard flow cytometer
Calibration bead size
0.2, 0.8
Detected EV-associated proteins
EMMPRIN/ EpCAM/ EGFR
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
187
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 6.05E+08
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV220300 | 3/18 | Homo sapiens | MCF7 |
UF (d)(U)C Filtration DG SEC (non-commercial) |
Pinheiro C | 2024 | 100% | |
Study summaryFull title
All authors
Pinheiro C, Guilbert N, Lippens L, Roux Q, Boiy R, Fischer S, Van Dorpe S, De Craene B, Berx G, Boterberg T, Sys G, Denys H, Miinalainen I, Mestdagh P, Vandesompele J, De Wever O, Hendrix A
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) contain a plethora of biomolecules, including nucleic acids, with diver (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Ultrafiltration
(Differential) (ultra)centrifugation Filtration Density gradient Size-exclusion chromatography (non-commercial) Protein markers
EV: Alix/ CD9/ Flotillin-1/ TSG101/ Syntenin
non-EV: Argonaute 2 Proteomics
no
EV density (g/ml)
1.09-1.11
Show all info
Study aim
Validation of standards
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MCF7
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
96
Cell count
4.43e8
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
15.5
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
16
Pelleting: speed (g)
100000
Filtration steps
Between 0.22 and 0.45 _m
Ultra filtration
Cut-off size (kDa)
10 kDa
Membrane type
Regenerated cellulose
Size-exclusion chromatography
Resin type
EV-subtype
Distinction between multiple subtypes
Size
Used subtypes
120-250
Characterization: Protein analysis
Protein Concentration Method
Not determined
Protein Yield (µg)
particles per milliliter of starting sample: 1.54E11-2.60E11
Western Blot
Detected EV-associated proteins
Alix/ CD9/ Flotillinð1/ TSG101/ Syntenin
Not detected contaminants
Argonauteð2
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Proteinase treatment
Yes
Moment of Proteinase treatment
After
Proteinase type
Proteinase K
Proteinase concentration
2
RNAse treatment
Yes
RNAse type
RNase A
RNAse concentration
8
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
125.7-134.7
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.54E11-2.60E11
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV220300 | 4/18 | Homo sapiens | A549 |
UF (d)(U)C Filtration DG |
Pinheiro C | 2024 | 89% | |
Study summaryFull title
All authors
Pinheiro C, Guilbert N, Lippens L, Roux Q, Boiy R, Fischer S, Van Dorpe S, De Craene B, Berx G, Boterberg T, Sys G, Denys H, Miinalainen I, Mestdagh P, Vandesompele J, De Wever O, Hendrix A
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) contain a plethora of biomolecules, including nucleic acids, with diver (show more...)
EV-METRIC
89% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Ultrafiltration
(Differential) (ultra)centrifugation Filtration Density gradient Protein markers
EV: Alix/ CD9/ TSG101
non-EV: None Proteomics
no
EV density (g/ml)
1.09-1.11
Show all info
Study aim
Validation of standards
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
A549
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
93
Cell count
1.09e8
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW32.1 Ti
Pelleting: speed (g)
100000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
15.5
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
16
Pelleting: speed (g)
100000
Filtration steps
Between 0.22 and 0.45 _m
Ultra filtration
Cut-off size (kDa)
10 kDa
Membrane type
Regenerated cellulose
Size-exclusion chromatography
Resin type
Characterization: Protein analysis
Protein Concentration Method
Not determined
Protein Yield (µg)
particles per milliliter of starting sample: 9.88E10
Western Blot
Detected EV-associated proteins
Alix/ CD9/ TSG101
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
186.1
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 9.88E10
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230598 | 1/3 | Homo sapiens | Milk | (d)(U)C | Ten-Doménech, Isabel | 2024 | 78% | |
Study summaryFull title
All authors
Isabel Ten-Doménech, Victoria Ramos-Garcia, Abel Albiach-Delgado, Jose Luis Moreno-Casillas, Alba Moreno-Giménez, María Gormaz, Marta Gómez-Ferrer, Pilar Sepúlveda, Máximo Vento, Guillermo Quintás, Julia Kuligowski
Journal
Chemometrics and Intelligent Laboratory Systems
Abstract
Human milk (HM) extracellular vesicles (EVs) are nano-sized, cell-derived particles sheathed in a li (show more...)
EV-METRIC
78% (86th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Sample origin
Mothers of preterm infants
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD9/ CD63/ CD81/ HSP70
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
108763
Wash: volume per pellet (ml)
25
Wash: time (min)
120
Wash: Rotor Type
Type 50.2 Ti
Wash: speed (g)
108763
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of EV isolate
Western Blot
Detected EV-associated proteins
CD9/ CD63/ CD81/ HSP70
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
168-238
EV concentration
Yes
Particle yield
as number of particles per milliliter of EV isolate: 1.4E10-1.4E12
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Other particle analysis name(1)
ExoView
Report type
Mean
Report size
58-70
EV-concentration
Yes
Particle yield
as number of particles per milliliter of EV isolate: 6E07-3E08
|
||||||||
EV230372 | 3/14 | Homo sapiens | HCC-78 |
(d)(U)C DG |
Schöne N | 2024 | 78% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
large EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: Actinin-4/ Rgap1/ Syntenin-1/ CD81/ EMMPRIN/ EpCAM/ EGFR/ Mitofilin,Arf6
non-EV: GM130/ HDAC1/ Albumin/ ApoA1/ ApoB Proteomics
no
EV density (g/ml)
1.10-1.17
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HCC-78
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
17000
Wash: volume per pellet (ml)
1
Wash: time (min)
30
Wash: Rotor Type
Heraeus 3331
Wash: speed (g)
17000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
16.5
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
16
Pelleting: speed (g)
100000
Pelleting-wash: volume per pellet (mL)
16
Pelleting-wash: duration (min)
60
Pelleting-wash: speed (g)
SW 32 Ti
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
Actinin-4/ Rgap1/ Syntenin-1/ Arf6/ Mitofilin
Not detected EV-associated proteins
CD81
Not detected contaminants
GM130/ HDAC1/ Albumin/ ApoA1/ ApoB
Flow cytometry
Type of Flow cytometry
standard flow cytometer
Calibration bead size
0.2, 0.8
Detected EV-associated proteins
EMMPRIN/ EpCAM/ EGFR
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
200.13
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 5.63E+09
|
||||||||
EV230372 | 8/14 | Homo sapiens | H2228 | (d)(U)C | Schöne N | 2024 | 78% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Syntenin/ Actinin-4/ Arf6/ Rgap1/ Mitofilin
non-EV: GM130/ HDAC1/ Albumin/ ApoA1/ ApoB Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
H2228
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
143000
Wash: volume per pellet (ml)
1.4
Wash: time (min)
90
Wash: Rotor Type
TLA-55
Wash: speed (g)
143000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
Syntenin/ Arf6/ CD81
Not detected EV-associated proteins
Actinin-4/ Rgap1/ Mitofilin
Not detected contaminants
GM130/ HDAC1/ Albumin/ ApoA1/ ApoB
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
163.8
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 2.10E+10
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230372 | 10/14 | Homo sapiens | H596 |
(d)(U)C DG |
Schöne N | 2024 | 78% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: CD81/ Syntenin/ Actinin-4/ Arf6/ Rgap1/ Mitofilin
non-EV: GM130/ HDAC1/ Albumin/ ApoA1/ ApoB Proteomics
no
EV density (g/ml)
1.10-1.17
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
H596
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
143000
Wash: volume per pellet (ml)
1.4
Wash: time (min)
90
Wash: Rotor Type
TLA-55
Wash: speed (g)
143000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
16.5
Sample volume (mL)
1
Orientation
Top-down
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
16
Pelleting: speed (g)
100000
Pelleting-wash: volume per pellet (mL)
16
Pelleting-wash: duration (min)
60
Pelleting-wash: speed (g)
SW 32 Ti
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
Syntenin/ Actinin-4/ Arf6/ CD81
Not detected EV-associated proteins
Rgap1/ Mitofilin
Not detected contaminants
GM130/ HDAC1/ Albumin/ ApoA1/ ApoB
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
141.2
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 6.09E+09
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV220326 | 1/4 | Homo sapiens | Glioblastoma stem-like cells NCH421k |
(d)(U)C DC |
Lokumcu T | 2024 | 78% | |
Study summaryFull title
All authors
Lokumcu T, Iskar M, Schneider M, Helm D, Klinke G, Schlicker L, Bethke F, Müller G, Richter K, Poschet G, Phillips E, Goidts V
Journal
ACS Nano
Abstract
Glioblastoma is a deadly brain tumor for which there is no cure. The presence of glioblastoma stem-l (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small extracellular vesicles (sEVs)
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
non-EV: Albumin/ Calreticulin/ Calnexin/ Argonaute-2/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein/ Cytochrome c1/ Lamin B1/ 60S acidic ribosomal protein P0 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Glioblastoma stem-like cells NCH421k
EV-harvesting Medium
Serum free medium
Cell viability (%)
91 - 93
Cell count
194200000 - 222312000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
10
Wash: time (min)
70
Wash: Rotor Type
SW 40 Ti
Wash: speed (g)
100000
Density cushion
Density medium
Iodixanol
Sample volume
7
Cushion volume
4
Density of the cushion
20%
Centrifugation time
70
Centrifugation speed
100000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per million cells
Western Blot
Detected EV-associated proteins
Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
Detected contaminants
60S acidic ribosomal protein P0 (RPLP0)
Not detected contaminants
GM130/ Lamin B1/ Cytochrome c
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Calreticulin/ Calnexin
Not detected contaminants
Argonaute 2/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein/ Cytochrome c1
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
175.0
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 4,58E+12
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV220326 | 2/4 | Homo sapiens | Glioblastoma stem-like cells NCH644 |
(d)(U)C DC |
Lokumcu T | 2024 | 78% | |
Study summaryFull title
All authors
Lokumcu T, Iskar M, Schneider M, Helm D, Klinke G, Schlicker L, Bethke F, Müller G, Richter K, Poschet G, Phillips E, Goidts V
Journal
ACS Nano
Abstract
Glioblastoma is a deadly brain tumor for which there is no cure. The presence of glioblastoma stem-l (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small extracellular vesicles (sEVs)
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
non-EV: Albumin/ Calreticulin/ Calnexin/ Argonaute-2/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein/ Cytochrome c1/ Lamin B1/ 60S acidic ribosomal protein P0 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Glioblastoma stem-like cells NCH644
EV-harvesting Medium
Serum free medium
Cell viability (%)
83 - 90
Cell count
225576000 - 231070000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
10
Wash: time (min)
70
Wash: Rotor Type
SW 40 Ti
Wash: speed (g)
100000
Density cushion
Density medium
Iodixanol
Sample volume
7
Cushion volume
4
Density of the cushion
20%
Centrifugation time
70
Centrifugation speed
100000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per million cells
Western Blot
Detected EV-associated proteins
Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
Detected contaminants
60S acidic ribosomal protein P0
Not detected contaminants
GM130/ Lamin B1/ Cytochrome c
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Calreticulin/ Calnexin
Not detected contaminants
Argonaute 2/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein/ Cytochrome c1
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
169.7
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1,87E+12
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV220326 | 3/4 | Homo sapiens | Glioblastoma stem-like cells NCH705 |
(d)(U)C DC |
Lokumcu T | 2024 | 78% | |
Study summaryFull title
All authors
Lokumcu T, Iskar M, Schneider M, Helm D, Klinke G, Schlicker L, Bethke F, Müller G, Richter K, Poschet G, Phillips E, Goidts V
Journal
ACS Nano
Abstract
Glioblastoma is a deadly brain tumor for which there is no cure. The presence of glioblastoma stem-l (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small extracellular vesicles (sEVs)
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
non-EV: Albumin/ Calreticulin/ Calnexin/ Argonaute-2/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein/ Cytochrome c1/ Lamin B1/ 60S acidic ribosomal protein P0 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Glioblastoma stem-like cells NCH705
EV-harvesting Medium
Serum free medium
Cell viability (%)
98
Cell count
204312000 - 225840000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
10
Wash: time (min)
70
Wash: Rotor Type
SW 40 Ti
Wash: speed (g)
100000
Density cushion
Density medium
Iodixanol
Sample volume
7
Cushion volume
4
Density of the cushion
20%
Centrifugation time
70
Centrifugation speed
100000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per million cells
Western Blot
Detected EV-associated proteins
Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
Detected contaminants
60S acidic ribosomal protein P0 (RPLP0)
Not detected contaminants
GM130/ Lamin B1/ Cytochrome c
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Calreticulin/ Calnexin
Not detected contaminants
Argonaute 2/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein/ Cytochrome c1
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
179.7
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 6,05E+12
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV220326 | 4/4 | Homo sapiens | Glioblastoma stem-like cells NCH711d |
(d)(U)C DC |
Lokumcu T | 2024 | 78% | |
Study summaryFull title
All authors
Lokumcu T, Iskar M, Schneider M, Helm D, Klinke G, Schlicker L, Bethke F, Müller G, Richter K, Poschet G, Phillips E, Goidts V
Journal
ACS Nano
Abstract
Glioblastoma is a deadly brain tumor for which there is no cure. The presence of glioblastoma stem-l (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small extracellular vesicles (sEVs)
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
non-EV: Albumin/ Calreticulin/ Calnexin/ Argonaute-2/ GM130/ PMP70/ Tamm-Horsfall protein/ Cytochrome c1/ Lamin B1/ 60S acidic ribosomal protein P0 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Glioblastoma stem-like cells NCH711d
EV-harvesting Medium
Serum free medium
Cell viability (%)
89 - 91
Cell count
102240000 - 116112000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
10
Wash: time (min)
70
Wash: Rotor Type
SW 40 Ti
Wash: speed (g)
100000
Density cushion
Density medium
Iodixanol
Sample volume
7
Cushion volume
4
Density of the cushion
20%
Centrifugation time
70
Centrifugation speed
100000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per million cells
Western Blot
Detected EV-associated proteins
Alix/ CD9/ TSG101/ Syndecan-1/ Enolase 1
Detected contaminants
60S acidic ribosomal protein P0 (RPLP0)
Not detected contaminants
GM130/ Lamin B1/ Cytochrome c
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Calreticulin/ Calnexin
Not detected contaminants
Argonaute 2/ GM130/ PMP70/ Tamm-Horsfall protein/ Cytochrome c1
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
203.5
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 2,87E+12
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV231008 | 1/27 | Homo sapiens | malignant ascites |
(d)(U)C DC |
Vyhlídalová Kotrbová A | 2024 | 75% | |
Study summaryFull title
All authors
Vyhlídalová Kotrbová A, Gömöryová K, Mikulová A, Plešingerová H, Sladeček S, Kravec M, Hrachovinová Š, Potěšil D, Dunsmore G, Blériot C, Bied M, Kotouček J, Bednaříková M, Hausnerová J, Minář L, Crha I, Felsinger M, Zdráhal Z, Ginhoux F, Weinberger V, Bryja V, Pospíchalová V
Journal
J Extracell Vesicles
Abstract
High-grade serous carcinoma of the ovary, fallopian tube and peritoneum (HGSC), the most common type (show more...)
EV-METRIC
75% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
malignant ascites
Sample origin
ovarian cancer (HGSC) patient 1
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: CD9/ CD81/ Flotillin-1/ Flotillin-2
non-EV: Apolipoprotein A-1/ Albumin/ Calreticulin/ GM130/ PMP70/ Argonaute-2/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
malignant ascites
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Density cushion
Density medium
Sucrose
Sample volume
34
Cushion volume
4
Density of the cushion
30%
Centrifugation time
70
Centrifugation speed
100,000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
CD9/ CD81/ Flotillin-1/ Flotillin-2
Detected contaminants
Apolipoprotein A-1
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Calreticulin/ GM130/ PMP70/ Apolipoprotein A-1
Not detected contaminants
Argonaute-2/ Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean±SD
Reported size (nm)
188±24
Used for determining EV concentration?
Yes
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV231008 | 2/27 | Homo sapiens | malignant ascites |
(d)(U)C UF qEV |
Vyhlídalová Kotrbová A | 2024 | 75% | |
Study summaryFull title
All authors
Vyhlídalová Kotrbová A, Gömöryová K, Mikulová A, Plešingerová H, Sladeček S, Kravec M, Hrachovinová Š, Potěšil D, Dunsmore G, Blériot C, Bied M, Kotouček J, Bednaříková M, Hausnerová J, Minář L, Crha I, Felsinger M, Zdráhal Z, Ginhoux F, Weinberger V, Bryja V, Pospíchalová V
Journal
J Extracell Vesicles
Abstract
High-grade serous carcinoma of the ovary, fallopian tube and peritoneum (HGSC), the most common type (show more...)
EV-METRIC
75% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
malignant ascites
Sample origin
ovarian cancer (HGSC) patient 1
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Ultrafiltration qEV Protein markers
EV: CD9/ CD81/ Flotillin-1/ Flotillin-2
non-EV: Apolipoprotein A-1/ Albumin/ Calreticulin/ GM130/ PMP70/ Prohibitin/ Argonaute-2/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
malignant ascites
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Polyethersulfone (PES)
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
CD9/ CD81/ Flotillin-1/ Flotillin-2
Detected contaminants
Apolipoprotein A-1
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Calreticulin/ GM130/ PMP70/ Prohibitin
Not detected contaminants
Argonaute-2/ Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean±SD
Reported size (nm)
689±205
Used for determining EV concentration?
Yes
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV231008 | 3/27 | Homo sapiens | malignant ascites |
(d)(U)C DC |
Vyhlídalová Kotrbová A | 2024 | 75% | |
Study summaryFull title
All authors
Vyhlídalová Kotrbová A, Gömöryová K, Mikulová A, Plešingerová H, Sladeček S, Kravec M, Hrachovinová Š, Potěšil D, Dunsmore G, Blériot C, Bied M, Kotouček J, Bednaříková M, Hausnerová J, Minář L, Crha I, Felsinger M, Zdráhal Z, Ginhoux F, Weinberger V, Bryja V, Pospíchalová V
Journal
J Extracell Vesicles
Abstract
High-grade serous carcinoma of the ovary, fallopian tube and peritoneum (HGSC), the most common type (show more...)
EV-METRIC
75% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
malignant ascites
Sample origin
ovarian cancer (HGSC) patient 2
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: CD9/ CD81/ Flotillin-1/ Flotillin-2
non-EV: Apolipoprotein A-1/ Albumin/ Argonaute-2/ Calreticulin/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
malignant ascites
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Density cushion
Density medium
Sucrose
Sample volume
34
Cushion volume
4
Density of the cushion
30%
Centrifugation time
70
Centrifugation speed
100,000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
CD9/ CD81/ Flotillin-1/ Flotillin-2
Detected contaminants
Apolipoprotein A-1
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Argonaute-2/ Calreticulin/ GM130/ PMP70/ Prohibitin/ Apolipoprotein A-1
Not detected contaminants
Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean±SD
Reported size (nm)
200±3
Used for determining EV concentration?
Yes
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV231008 | 4/27 | Homo sapiens | malignant ascites |
(d)(U)C UF qEV |
Vyhlídalová Kotrbová A | 2024 | 75% | |
Study summaryFull title
All authors
Vyhlídalová Kotrbová A, Gömöryová K, Mikulová A, Plešingerová H, Sladeček S, Kravec M, Hrachovinová Š, Potěšil D, Dunsmore G, Blériot C, Bied M, Kotouček J, Bednaříková M, Hausnerová J, Minář L, Crha I, Felsinger M, Zdráhal Z, Ginhoux F, Weinberger V, Bryja V, Pospíchalová V
Journal
J Extracell Vesicles
Abstract
High-grade serous carcinoma of the ovary, fallopian tube and peritoneum (HGSC), the most common type (show more...)
EV-METRIC
75% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
malignant ascites
Sample origin
ovarian cancer (HGSC) patient 2
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Ultrafiltration qEV Protein markers
EV: CD9/ CD81/ Flotillin-1/ Flotillin-2
non-EV: Apolipoprotein A-1/ Albumin/ Calreticulin/ PMP70/ Prohibitin/ Argonaute-2/ GM130/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
malignant ascites
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Polyethersulfone (PES)
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
CD9/ CD81/ Flotillin-1/ Flotillin-2
Detected contaminants
Apolipoprotein A-1
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Calreticulin/ PMP70/ Prohibitin/ Apolipoprotein A-1
Not detected contaminants
Argonaute-2/ GM130/ Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean±SD
Reported size (nm)
1009±417
Used for determining EV concentration?
Yes
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV240137 | 2/4 | Mus musculus | Blood plasma |
(d)(U)C Filtration |
Arteaga-Blanco, Luis A. | 2024 | 67% | |
Study summaryFull title
All authors
Luis A. Arteaga-Blanco, Andrew E. Evans, Dan A. Dixon
Journal
Cells
Abstract
NA (show more...)
EV-METRIC
67% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
small extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD63/ CD81/ actin-beta
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
S55-S
Pelleting: speed (g)
150000
Wash: volume per pellet (ml)
2
Wash: time (min)
70
Wash: Rotor Type
S55-S
Wash: speed (g)
150000
Filtration steps
0.8 µm/ 0.2 or 0.22 µm
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Western Blot
Detected EV-associated proteins
CD63/ CD81/ actin-beta
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
108
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 4.10E+08
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
Report size (nm)
120
|
||||||||
EV240137 | 4/4 | Mus musculus | Blood plasma |
(d)(U)C Filtration |
Arteaga-Blanco, Luis A. | 2024 | 67% | |
Study summaryFull title
All authors
Luis A. Arteaga-Blanco, Andrew E. Evans, Dan A. Dixon
Journal
Cells
Abstract
NA (show more...)
EV-METRIC
67% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
APCMin/+ CRC mice model
Focus vesicles
small extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD63/ CD81/ actin-beta
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
S55-S
Pelleting: speed (g)
150000
Wash: volume per pellet (ml)
2
Wash: time (min)
70
Wash: Rotor Type
S55-S
Wash: speed (g)
150000
Filtration steps
0.8 µm/ 0.2 or 0.22 µm
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Western Blot
Detected EV-associated proteins
CD63/ CD81/ actin-beta
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
120
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.36E+09
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
Report size (nm)
132
|
||||||||
EV231008 | 5/27 | Homo sapiens | malignant ascites |
(d)(U)C DC |
Vyhlídalová Kotrbová A | 2024 | 67% | |
Study summaryFull title
All authors
Vyhlídalová Kotrbová A, Gömöryová K, Mikulová A, Plešingerová H, Sladeček S, Kravec M, Hrachovinová Š, Potěšil D, Dunsmore G, Blériot C, Bied M, Kotouček J, Bednaříková M, Hausnerová J, Minář L, Crha I, Felsinger M, Zdráhal Z, Ginhoux F, Weinberger V, Bryja V, Pospíchalová V
Journal
J Extracell Vesicles
Abstract
High-grade serous carcinoma of the ovary, fallopian tube and peritoneum (HGSC), the most common type (show more...)
EV-METRIC
67% (72nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
malignant ascites
Sample origin
ovarian cancer (HGSC) patient 3
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: None
non-EV: Albumin/ Argonaute-2/ Calreticulin/ GM130/ PMP70/ Prohibitin/ Apolipoprotein A-1/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
malignant ascites
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Density cushion
Density medium
Sucrose
Sample volume
34
Cushion volume
4
Density of the cushion
30%
Centrifugation time
70
Centrifugation speed
100,000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Argonaute-2/ Calreticulin/ GM130/ PMP70/ Prohibitin/ Apolipoprotein A-1
Not detected contaminants
Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean±SD
Reported size (nm)
403±47
Used for determining EV concentration?
Yes
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV231008 | 6/27 | Homo sapiens | malignant ascites |
(d)(U)C UF qEV |
Vyhlídalová Kotrbová A | 2024 | 67% | |
Study summaryFull title
All authors
Vyhlídalová Kotrbová A, Gömöryová K, Mikulová A, Plešingerová H, Sladeček S, Kravec M, Hrachovinová Š, Potěšil D, Dunsmore G, Blériot C, Bied M, Kotouček J, Bednaříková M, Hausnerová J, Minář L, Crha I, Felsinger M, Zdráhal Z, Ginhoux F, Weinberger V, Bryja V, Pospíchalová V
Journal
J Extracell Vesicles
Abstract
High-grade serous carcinoma of the ovary, fallopian tube and peritoneum (HGSC), the most common type (show more...)
EV-METRIC
67% (72nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
malignant ascites
Sample origin
ovarian cancer (HGSC) patient 3
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Ultrafiltration qEV Protein markers
EV: None
non-EV: Albumin/ Calreticulin/ PMP70/ Prohibitin/ Apolipoprotein A-1/ Argonaute-2/ GM130/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
malignant ascites
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Polyethersulfone (PES)
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Calreticulin/ PMP70/ Prohibitin/ Apolipoprotein A-1
Not detected contaminants
Argonaute-2/ GM130/ Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean±SD
Reported size (nm)
730±158
Used for determining EV concentration?
Yes
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV231008 | 7/27 | Homo sapiens | malignant ascites |
(d)(U)C DC |
Vyhlídalová Kotrbová A | 2024 | 67% | |
Study summaryFull title
All authors
Vyhlídalová Kotrbová A, Gömöryová K, Mikulová A, Plešingerová H, Sladeček S, Kravec M, Hrachovinová Š, Potěšil D, Dunsmore G, Blériot C, Bied M, Kotouček J, Bednaříková M, Hausnerová J, Minář L, Crha I, Felsinger M, Zdráhal Z, Ginhoux F, Weinberger V, Bryja V, Pospíchalová V
Journal
J Extracell Vesicles
Abstract
High-grade serous carcinoma of the ovary, fallopian tube and peritoneum (HGSC), the most common type (show more...)
EV-METRIC
67% (72nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
malignant ascites
Sample origin
ovarian cancer (HGSC) patient 4
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Protein markers
EV: None
non-EV: Albumin/ Calreticulin/ GM130/ PMP70/ Prohibitin/ Apolipoprotein A-1/ Argonaute-2/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
malignant ascites
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Density cushion
Density medium
Sucrose
Sample volume
34
Cushion volume
4
Density of the cushion
30%
Centrifugation time
70
Centrifugation speed
100,000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Calreticulin/ GM130/ PMP70/ Prohibitin/ Apolipoprotein A-1
Not detected contaminants
Argonaute-2/ Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean±SD
Reported size (nm)
222±22
Used for determining EV concentration?
Yes
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV231008 | 8/27 | Homo sapiens | malignant ascites |
(d)(U)C UF qEV |
Vyhlídalová Kotrbová A | 2024 | 67% | |
Study summaryFull title
All authors
Vyhlídalová Kotrbová A, Gömöryová K, Mikulová A, Plešingerová H, Sladeček S, Kravec M, Hrachovinová Š, Potěšil D, Dunsmore G, Blériot C, Bied M, Kotouček J, Bednaříková M, Hausnerová J, Minář L, Crha I, Felsinger M, Zdráhal Z, Ginhoux F, Weinberger V, Bryja V, Pospíchalová V
Journal
J Extracell Vesicles
Abstract
High-grade serous carcinoma of the ovary, fallopian tube and peritoneum (HGSC), the most common type (show more...)
EV-METRIC
67% (72nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
malignant ascites
Sample origin
ovarian cancer (HGSC) patient 4
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Ultrafiltration qEV Protein markers
EV: None
non-EV: Albumin/ Calreticulin/ GM130/ PMP70/ Prohibitin/ Apolipoprotein A-1/ Argonaute-2/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
malignant ascites
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Polyethersulfone (PES)
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
ProteomeXchange
Detected contaminants
Albumin/ Calreticulin/ GM130/ PMP70/ Prohibitin/ Apolipoprotein A-1
Not detected contaminants
Argonaute-2/ Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean±SD
Reported size (nm)
509±115
Used for determining EV concentration?
Yes
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV230995 | 1/6 | Homo sapiens | MML-1 | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD63/ CD81/ Flotillin-1
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MML-1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
94
Cell count
50000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
16500
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
CD63/ CD81/ Flotillin-1
Detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 761800000
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230995 | 2/6 | Homo sapiens | UM22Bap1+/+ | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD63/ CD81/ Flotillin-1
non-EV: Calnexin/ bovine HBA1c/ bovine CPN1 Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
UM22Bap1+/+
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
94
Cell count
50000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
16500
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
CD63/ CD81/ Flotillin-1
Not detected contaminants
Calnexin
ELISA
Detected contaminants
bovine HBA1c/ bovine CPN1
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 936666666.7
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230995 | 3/6 | Homo sapiens | UM22Bap1-/- | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Flotillin-1/ CD63
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
UM22Bap1-/-
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
93
Cell count
50000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
16500
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
CD81/ Flotillin-1
Not detected EV-associated proteins
CD63
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 36277777.78
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230995 | 4/6 | Homo sapiens | MML-1 | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD63/ CD81/ Flotillin-1
non-EV: Calnexin/ bovine CPN1 Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MML-1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
94
Cell count
50000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
CD63/ CD81/ Flotillin-1
Not detected contaminants
Calnexin
ELISA
Detected contaminants
bovine CPN1
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 139727777.8
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230995 | 5/6 | Homo sapiens | UM22Bap1+/+ | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD63/ CD81/ Flotillin-1
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
UM22Bap1+/+
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
94
Cell count
50000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
CD63/ CD81/ Flotillin-1
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 82011111.11
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230995 | 6/6 | Homo sapiens | UM22Bap1-/- | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD63/ CD81/ Flotillin-1
non-EV: Calnexin/ bovine CPN1 Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
UM22Bap1-/-
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
93
Cell count
50000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
CD63/ CD81/ Flotillin-1
Not detected contaminants
Calnexin
ELISA
Detected contaminants
bovine CPN1
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 342605555.6
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230994 | 2/6 | Bos taurus | Commercially available FBS | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Commercially available FBS
Sample origin
HI-before EV-depl
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: HSP70/ HSP90/ HBA1
non-EV: Albumin/ Argonaute-2/ Calreticulin/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Bos taurus
Sample Type
Commercially available FBS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
16500
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
HSP70/ HSP90/ HBA1
Proteomics database
No
Detected contaminants
Albumin/ Argonaute-2/ Calreticulin
Not detected contaminants
GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 32260000
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230994 | 4/6 | Bos taurus | Commercially available FBS | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Commercially available FBS
Sample origin
no-HI
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: HSP70/ HSP90/ HBA1
non-EV: Albumin/ Argonaute-2/ Calreticulin/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Bos taurus
Sample Type
Commercially available FBS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
HSP70/ HSP90/ HBA1
Proteomics database
No
Detected contaminants
Albumin/ Argonaute-2/ Calreticulin
Not detected contaminants
GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 148220000
EM
EM-type
Transmission-EM
Image type
Close-up
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EV230994 | 6/6 | Bos taurus | Commercially available FBS | (d)(U)C | Urzì O | 2024 | 67% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
67% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Commercially available FBS
Sample origin
HI-after EV-depl
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: HSP70/ HSP90/ HBA1
non-EV: Albumin/ Argonaute-2/ Calreticulin/ GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein Proteomics
yes
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Bos taurus
Sample Type
Commercially available FBS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
HSP70/ HSP90/ HBA1
Proteomics database
No
Detected contaminants
Albumin/ Argonaute-2/ Calreticulin
Not detected contaminants
GM130/ PMP70/ Prohibitin/ Tamm-Horsfall protein
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 133940000
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230572 | 3/6 | Homo sapiens | HEK293-GFP | (d)(U)C | Djeungoue-Petga, Marie-Ange | 2024 | 67% | |
Study summaryFull title
All authors
Marie Ange Djeungoue Petgaa, Catherine Taylora, Alexander Macpherson, Surendar Reddy Dhadi, Thomas Rollin, Jeremy W. Roya, Anirban Ghosh, Stephen M. Lewis, Rodney J. Ouellette
Journal
Abstract
Extracellular vesicles (EVs) are gaining interest as efficient, biocompatible vehicles for cellular (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
GFP overexpression
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD9/ CD63/ Flotillin-1/ HSP70/ GFP
non-EV: GRP94 Proteomics
no
Show all info
Study aim
Mechanism of uptake/transfer/New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HEK293-GFP
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
SW 40 Ti
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
CD9/ CD63/ Flotillin-1/ HSP70/ GFP
Not detected contaminants
GRP94
Flow cytometry
Type of Flow cytometry
Beckman Coulter Cytoflex
Hardware adaptation to ~100nm EV's
The better resolution of the CytoFLEX is reached by using the violet side scatter of the 405 nm laser (manually set to 1600 and height threshold) and by performing preanalytical preparations with Fluorescent Megamix-Plus SSC beads (Cosmo Bio Co., LTD, Japan) which are FITC-labeled beads of increasing size (100, 160, 200, 240, 300, 500, 900 nm). beads were used to set the EV gate and manual gating was set to the populations of interest with reference to a negative control sample (GFP- EVs from HEK293 cells)
Calibration bead size
0.1/ 0.16/ 0.2/ 0.24/ 0.3/ 0.5/ 0.9
Detected EV-associated proteins
GFP
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
~172
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 6.00E+09
Particle analysis: flow cytometry
Flow cytometer type
Nanoscale
Hardware adjustment
The better resolution of the CytoFLEX is reached by using the violet side scatter of the 405 nm laser (manually set to 1600 and height threshold) and by performing preanalytical preparations with Fluorescent Megamix-Plus SSC beads (Cosmo Bio Co., LTD, Japan) which are FITC-labeled beads of increasing size (100, 160, 200, 240, 300, 500, 900 nm). beads were used to set the EV gate and manual gating was set to the populations of interest with reference to a negative control sample (GFP- EVs from HEK293 cells)
Calibration bead size
0.1/ 0.16/ 0.2/ 0.24/ 0.3/ 0.5/ 0.9
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 9.00E+06
EM
EM-type
Transmission-EM
Image type
Close-up
|
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EV230029 | 1/2 | Homo sapiens | MIA PaCa-2 |
(d)(U)C EX01-25L Exo-spin Standard Kit |
Nannan, Lise | 2024 | 67% | |
Study summaryFull title
All authors
Lise Nannan, Salomé Decombis, Christine Terryn, Sandra Audonnet, Jean Michel, Sylvie Brassart-Pasco, Willy Gsell, Uwe Himmelreich, Bertrand Brassart
Journal
J Extracell Biol
Abstract
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy with poor prognosis due to its h (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
eGFP/FLuc overexpression
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Protein markers
EV: CD9/ CD63/ CD81
non-EV: GM130 Proteomics
no
Show all info
Study aim
Function/Biomarker/Mechanism of uptake/transfer/New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MIA PaCa-2
EV-harvesting Medium
Serum free medium
Cell viability (%)
97
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
0.1
Wash: time (min)
70
Wash: Rotor Type
TLA-100.4
Wash: speed (g)
100000
Commercial kit
EX01-25L Exo-spin Standard Kit
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD9/ CD63/ CD81
Not detected contaminants
GM130
Flow cytometry aspecific beads
Detected EV-associated proteins
CD9/ CD63/ CD81
Flow cytometry specific beads
Selected surface protein(s)
CD9/ CD63/ CD81
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Proteinase treatment
No
RNAse treatment
Yes
RNAse type
RNase A
RNAse concentration
0.004
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
120
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.50E+08
EM
EM-type
Transmission-EM/ Immuno-EM
EM protein
CD63
|