Search > Results
You searched for: EV230598 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV230598 | 1/3 | Homo sapiens | Milk | (d)(U)C | Ten-Doménech, Isabel | 2024 | 78% | |
Study summaryFull title
All authors
Isabel Ten-Doménech, Victoria Ramos-Garcia, Abel Albiach-Delgado, Jose Luis Moreno-Casillas, Alba Moreno-Giménez, María Gormaz, Marta Gómez-Ferrer, Pilar Sepúlveda, Máximo Vento, Guillermo Quintás, Julia Kuligowski
Journal
Chemometrics and Intelligent Laboratory Systems
Abstract
Human milk (HM) extracellular vesicles (EVs) are nano-sized, cell-derived particles sheathed in a li (show more...)
EV-METRIC
78% (86th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Sample origin
Mothers of preterm infants
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD9/ CD63/ CD81/ HSP70
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
108763
Wash: volume per pellet (ml)
25
Wash: time (min)
120
Wash: Rotor Type
Type 50.2 Ti
Wash: speed (g)
108763
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of EV isolate
Western Blot
Detected EV-associated proteins
CD9/ CD63/ CD81/ HSP70
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
168-238
EV concentration
Yes
Particle yield
as number of particles per milliliter of EV isolate: 1.4E10-1.4E12
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Other particle analysis name(1)
ExoView
Report type
Mean
Report size
58-70
EV-concentration
Yes
Particle yield
as number of particles per milliliter of EV isolate: 6E07-3E08
|
||||||||
EV230598 | 2/3 | Homo sapiens | Milk | (d)(U)C | Ten-Doménech, Isabel | 2024 | 56% | |
Study summaryFull title
All authors
Isabel Ten-Doménech, Victoria Ramos-Garcia, Abel Albiach-Delgado, Jose Luis Moreno-Casillas, Alba Moreno-Giménez, María Gormaz, Marta Gómez-Ferrer, Pilar Sepúlveda, Máximo Vento, Guillermo Quintás, Julia Kuligowski
Journal
Chemometrics and Intelligent Laboratory Systems
Abstract
Human milk (HM) extracellular vesicles (EVs) are nano-sized, cell-derived particles sheathed in a li (show more...)
EV-METRIC
56% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Sample origin
Mothers of term infants
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD9/ CD63/ CD81/ HSP70
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
108763
Wash: volume per pellet (ml)
25
Wash: time (min)
120
Wash: Rotor Type
Type 50.2 Ti
Wash: speed (g)
108763
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of EV isolate
Western Blot
Detected EV-associated proteins
CD9/ CD63/ CD81/ HSP70
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
168-238
EV concentration
Yes
Particle yield
as number of particles per milliliter of EV isolate: 1.4E10-1.4E12
Other particle analysis name(1)
ExoView
Report type
Mean
Report size
58-70
EV-concentration
Yes
Particle yield
as number of particles per milliliter of EV isolate: 6E07-3E08
|
||||||||
EV230598 | 3/3 | Homo sapiens | Milk | (d)(U)C | Ten-Doménech, Isabel | 2024 | 44% | |
Study summaryFull title
All authors
Isabel Ten-Doménech, Victoria Ramos-Garcia, Abel Albiach-Delgado, Jose Luis Moreno-Casillas, Alba Moreno-Giménez, María Gormaz, Marta Gómez-Ferrer, Pilar Sepúlveda, Máximo Vento, Guillermo Quintás, Julia Kuligowski
Journal
Chemometrics and Intelligent Laboratory Systems
Abstract
Human milk (HM) extracellular vesicles (EVs) are nano-sized, cell-derived particles sheathed in a li (show more...)
EV-METRIC
44% (67th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Sample origin
Pool of donors
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD9/ CD63/ CD81
non-EV: None Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
108763
Wash: volume per pellet (ml)
25
Wash: time (min)
120
Wash: Rotor Type
Type 50.2 Ti
Wash: speed (g)
108763
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of EV isolate
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
Other particle analysis name(1)
ExoView¨
Report type
Mean
Report size
58-70
EV-concentration
Yes
Particle yield
as number of particles per milliliter of EV isolate: 6E07-3E08
|
||||||||
1 - 3 of 3 |
EV-TRACK ID | EV230598 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Milk | ||
condition | Mothers of preterm infants | Mothers of term infants | Pool of donors |
separation protocol | dUC | dUC | dUC |
Exp. nr. | 1 | 2 | 3 |
EV-METRIC % | 78 | 56 | 44 |