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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV231007	1/1	Homo sapiens	Liver tissue conditioned media	STEMCELL SEC columns UF	Newman LA	2024	67%
Study summary Full title Establishing the capacity to monitor proteins relevant to the study of drug exposure and response using liver-derived extracellular vesicles. All authors Newman LA, Useckaite Z, Wu T, Sorich MJ, Rowland A Journal Br J Clin Pharmacol Abstract Drug exposure and response is determined by pharmacokinetic (PK) and pharmacodynamic (PD) profiles. (show more...)Drug exposure and response is determined by pharmacokinetic (PK) and pharmacodynamic (PD) profiles. Interindividual differences in abundance of drug metabolizing enzymes (DMEs) and drug target proteins underpin PK and PD variability and impact treatment efficacy and tolerability. Extracellular vesicles (EVs) carry protein cargo inherited from originating cells and may be useful for defining differences in key proteins related to hepatic drug metabolism and the treatment of metabolic-associated fatty liver disease (MAFLD). We sought to quantify these proteins in liver-derived EVs and establish the profile relative to paired tissue. (hide) EV-METRIC 67% (50th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Liver tissue conditioned media Sample origin Control condition Focus vesicles extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture STEMCELL SEC columns Ultrafiltration Protein markers EV: None non-EV: Albumin Proteomics yes Show all info Study aim Identification of content (omics approaches) Sample Species Homo sapiens Sample Type Liver tissue conditioned media Separation Method Ultra filtration Cut-off size (kDa) 30kDa Membrane type Regenerated cellulose Commercial kit STEMCELL SEC columns Other Name other separation method STEMCELL SEC columns Characterization: Protein analysis Protein Concentration Method microBCA Protein Yield (µg) per g of starting sample Proteomics database No Detected contaminants Albumin Characterization: Lipid analysis No Characterization: Particle analysis NTA Report type Mean Reported size (nm) 83 EV concentration Yes Particle yield particles per mg of starting sample: 6.65E+09 EM EM-type Transmission-EM Image type Close-up, Wide-field Report size (nm) 85

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EV-TRACK ID	EV231007
species	Homo sapiens
sample type	Liver tissue conditioned media
condition	Control condition
separation protocol	STEMCELL SEC columns/ Ultrafiltration
Exp. nr.	1
EV-METRIC %	67