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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV240026	1/2	Homo sapiens	Human Wharton's Jelly-Mesenchymal Stromal Cells	(d)(U)C	Seydi H	2024	78%
Study summary Full title MiR-29a-laden extracellular vesicles efficiently induced apoptosis through autophagy blockage in HCC cells. All authors Seydi H, Nouri K, Shokouhian B, Piryaei A, Hassan M, Cordani M, Zarrabi A, Shekari F, Vosough M Journal Eur J Pharm Biopharm Abstract In spite of significant advancements in theraputic modalities for hepatocellular carcinoma (HCC), th (show more...)In spite of significant advancements in theraputic modalities for hepatocellular carcinoma (HCC), there is still a high annual mortality rate with a rising incidence. Major challenges in the HCC clinical managment are related to the development of therapy resistance, and evasion of tumor cells apoptosis which leading unsatisfactory outcomes in HCC patients. Previous investigations have shown that autophagy plays crucial role in contributing to drug resistance development in HCC. Although, miR-29a is known to counteract authophagy, increasing evidence revealed a down-regulation of miR-29a in HCC patients which correlates with poor prognosis. Beside, evidences showed that miR-29a serves as a negative regulator of autophagy in other cancers. In the current study, we aim to investigate the impact of miR-29a on the autophagy and apoptosis in HCC cells using extracellular vesicles (EVs) as a natural delivery system given their potential in the miRNA delivery both in vitro and in vivo. (hide) EV-METRIC 78% (97th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Cell culture supernatant Sample origin Control condition Focus vesicles EV20K Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (Differential) (ultra)centrifugation Protein markers EV: CD9/ CD63/ CD81/ TSG101 non-EV: Albumin Proteomics no Show all info Study aim Function Sample Species Homo sapiens Sample Type Cell culture supernatant EV-producing cells Human Wharton's Jelly-Mesenchymal Stromal Cells EV-harvesting Medium Serum-containing medium Cell viability (%) 87 Cell count 4000000000 Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 10,000 g and 50,000 g Pelleting performed Yes Pelleting: rotor type NA-1HS Pelleting: speed (g) 20000 Wash: volume per pellet (ml) 1 Wash: time (min) 30 Wash: Rotor Type NA-1HS Wash: speed (g) 20000 Characterization: Protein analysis Protein Concentration Method BCA Protein Yield (µg) per million cells Western Blot Detected EV-associated proteins CD9/ CD63/ CD81/ TSG101 Not detected contaminants Albumin Characterization: RNA analysis RNA analysis Type (RT)(q)PCR Characterization: Lipid analysis No Characterization: Particle analysis DLS Report type Mean Reported size (nm) 332 EM EM-type Scanning-EM Image type Wide-field Report size (nm) 250.77

	EV240026	2/2	Homo sapiens	Human Wharton's Jelly-Mesenchymal Stromal Cells	(d)(U)C	Seydi H	2024	78%
Study summary Full title MiR-29a-laden extracellular vesicles efficiently induced apoptosis through autophagy blockage in HCC cells. All authors Seydi H, Nouri K, Shokouhian B, Piryaei A, Hassan M, Cordani M, Zarrabi A, Shekari F, Vosough M Journal Eur J Pharm Biopharm Abstract In spite of significant advancements in theraputic modalities for hepatocellular carcinoma (HCC), th (show more...)In spite of significant advancements in theraputic modalities for hepatocellular carcinoma (HCC), there is still a high annual mortality rate with a rising incidence. Major challenges in the HCC clinical managment are related to the development of therapy resistance, and evasion of tumor cells apoptosis which leading unsatisfactory outcomes in HCC patients. Previous investigations have shown that autophagy plays crucial role in contributing to drug resistance development in HCC. Although, miR-29a is known to counteract authophagy, increasing evidence revealed a down-regulation of miR-29a in HCC patients which correlates with poor prognosis. Beside, evidences showed that miR-29a serves as a negative regulator of autophagy in other cancers. In the current study, we aim to investigate the impact of miR-29a on the autophagy and apoptosis in HCC cells using extracellular vesicles (EVs) as a natural delivery system given their potential in the miRNA delivery both in vitro and in vivo. (hide) EV-METRIC 78% (97th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Cell culture supernatant Sample origin Control condition Focus vesicles EV110K Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (Differential) (ultra)centrifugation Protein markers EV: CD9/ CD63/ CD81/ TSG101 non-EV: Albumin Proteomics no Show all info Study aim Function Sample Species Homo sapiens Sample Type Cell culture supernatant EV-producing cells Human Wharton's Jelly-Mesenchymal Stromal Cells EV-harvesting Medium Serum-containing medium Cell viability (%) 87 Cell count 4000000000 Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 100,000 g and 150,000 g Pelleting performed Yes Pelleting: rotor type SW 32 Ti Pelleting: speed (g) 110000 Wash: volume per pellet (ml) 1 Wash: time (min) 120 Wash: Rotor Type SW 32 Ti Wash: speed (g) 110000 Characterization: Protein analysis Protein Concentration Method BCA Protein Yield (µg) per million cells Western Blot Detected EV-associated proteins CD9/ CD63/ CD81/ TSG101 Not detected contaminants Albumin Characterization: RNA analysis RNA analysis Type (RT)(q)PCR Characterization: Lipid analysis No Characterization: Particle analysis DLS Report type Mean Reported size (nm) 239 EM EM-type Scanning-EM Image type Wide-field

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EV-TRACK ID	EV240026
species	Homo sapiens
sample type	Cell culture
cell type	Human Wharton's Jelly-Mesenchymal Stromal Cells
condition	Control condition
separation protocol	dUC	dUC
vesicle related term	EV20K	EV110K
Exp. nr.	1	2
EV-METRIC %	78	78