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Showing 101 - 150 of 1113
Showing 101 - 150 of 1113
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV180079 | 2/2 | Mus musculus | B16 | (d)(U)C | Lucia Paolini | 2020 | 67% | |
Study summaryFull title
All authors
Lucia Paolini, Stefania Federici, Giovanni Consoli, Diletta Arceri, Annalisa Radeghieri, Ivano Alessandri, Paolo Bergese
Journal
J Extracell Vesicles
Abstract
Identification of extracellular vesicle (EV) subpopulations remains an open challenge. To date, the (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: ADAM10/ Annexin V/ Flotillin1/ CD81
non-EV: GM130 Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
B16
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
240
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
1
Wash: time (min)
120
Wash: Rotor Type
TLA-55
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Colorimetric Nanoplasmonic assay
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ Annexin V/ ADAM10/ CD81
Not detected contaminants
GM130
Characterization: Lipid analysis
Yes
EM
EM-type
Atomic force-EM
Image type
Close-up, Wide-field
Report size (nm)
large EVS 690 nm, medium Evs 230 nm, small Evs 50 nm
|
||||||||
EV180057 | 1/1 | Rattus norvegicus | rBMSC | (d)(U)C | Gissi, Clarissa | 2020 | 67% | |
Study summaryFull title
All authors
Clarissa Gissi, Annalisa Radeghieri, Cristina Antonetti Lamorgese Passeri, Marialucia Gallorini, Lucia Calciano, Francesco Oliva, Francesca Veronesi, Andrea Zendrini, Amelia Cataldi, Paolo Bergese, Nicola Maffulli, Anna Concetta Berardi
Journal
PLoS One
Abstract
Mesenchymal stromal/stem cells (MSCs) are increasingly employed for tissue regeneration, largely med (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD81/ Pro-collagen1A2/ Annexin/ TERT/ Annexin V
non-EV: Argonaute2/ GM130 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Rattus norvegicus
Sample Type
Cell culture supernatant
EV-producing cells
rBMSC
EV-harvesting Medium
Serum free medium
Cell viability (%)
100
Cell count
4.18x10e6
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 28
Pelleting: speed (g)
120000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD81/ Annexin V/ TERT/ Pro-collagen1A2
Detected contaminants
Argonaute2
Not detected contaminants
GM130
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Atomic force-EM
Image type
Wide-field
Report size (nm)
95
Report type
Not Reported
EV-concentration
Yes
Particle yield
1E11 per particles mL starting sample
|
||||||||
XL5296IL | 1/2 | Macaca mulatta | Cervicovaginal lavage fluid | (d)(U)C | Zhao, Zezhou | 2020 | 66% | |
Study summaryFull title
All authors
Zezhou Zhao, Dillon C Muth, Kathleen Mulka, Zhaohao Liao, Bonita H Powell, Grace V Hancock, Kelly A Metcalf Pate, Kenneth W Witwer
Journal
FEBS Open Bio
Abstract
Cervicovaginal secretions, or their components collected, are referred to as cervicovaginal lavage ( (show more...)
EV-METRIC
66% (66th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cervicovaginal lavage fluid
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
113.6 (pelleting)
Protein markers
EV: CD81/ CD63
non-EV: calnexin Proteomics
no
Show all info
Study aim
Biomarker, Identification of content (omics approaches)
Sample
Species
Macaca mulatta
Sample Type
Cervicovaginal lavage fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
AH-650
Pelleting: speed (g)
110000
Pelleting: adjusted k-factor
113.6
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63, CD81
Not detected contaminants
calnexin
Characterization: RNA analysis
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
xx-xxx
EV concentration
Yes
Particle yield
2.5E7-3.5E9
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
up to 200
Extra information
Sample volumes and yield limited the isolation and characterization steps we were able to perform.
|
||||||||
XL5296IL | 2/2 | Macaca mulatta | Cervicovaginal lavage fluid | (d)(U)C | Zhao, Zezhou | 2020 | 66% | |
Study summaryFull title
All authors
Zezhou Zhao, Dillon C Muth, Kathleen Mulka, Zhaohao Liao, Bonita H Powell, Grace V Hancock, Kelly A Metcalf Pate, Kenneth W Witwer
Journal
FEBS Open Bio
Abstract
Cervicovaginal secretions, or their components collected, are referred to as cervicovaginal lavage ( (show more...)
EV-METRIC
66% (66th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cervicovaginal lavage fluid
Sample origin
SIVmac251-infected
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
113.6 (pelleting)
Protein markers
EV: CD81/ CD63
non-EV: calnexin Proteomics
no
Show all info
Study aim
Biomarker, Identification of content (omics approaches)
Sample
Species
Macaca mulatta
Sample Type
Cervicovaginal lavage fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
AH-650
Pelleting: speed (g)
110000
Pelleting: adjusted k-factor
113.6
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63, CD81
Not detected contaminants
calnexin
Characterization: RNA analysis
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
xx-xxx
EV concentration
Yes
Particle yield
1.75E8-2.15E9
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
up to 200 nm
Extra information
Samples from SIV Infected animals were pooled for WB analysis
|
||||||||
EV200073 | 1/4 | Homo sapiens | Solid Tissue |
(d)(U)C Size-exclusion chromatopraphy (IZON) DC SEC (IZON) |
Bordas, Marie | 2020 | 63% | |
Study summaryFull title
All authors
Marie Bordas, Géraldine Genard, Sibylle Ohl, Michelle Nessling, Karsten Richter, Tobias Roider, Sascha Dietrich, Kendra K Maaß, Martina Seiffert
Journal
Int J Mol Sci
Abstract
Small extracellular vesicles (sEVs) are nanoparticles responsible for cell-to-cell communication rel (show more...)
EV-METRIC
63% (25th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Solid Tissue
Sample origin
CLL
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Size-exclusion chromatopraphy (IZON) DC SEC (IZON) Protein markers
EV: TSG101/ CD81/ Flotillin1/ CD9
non-EV: Cytochrome C/ GM130/ Calreticulin Proteomics
no
Show all info
Study aim
Function/New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Solid Tissue
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed
No
Density cushion
Density medium
Sucrose
Other
Name other separation method
Size-exclusion chromatopraphy (IZON)
Other
Name other separation method
SEC (IZON)
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Flotillin1/ CD9/ TSG101/ CD81
Detected contaminants
Calreticulin
Not detected contaminants
Cytochrome C/ GM130
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
150
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 4.00E+10
EM
EM-type
Immuno-EM/ Transmission-EM
EM protein
Other;HLA-DR
Image type
Close-up
|
||||||||
EV200073 | 3/4 | Mus musculus | Solid Tissue |
(d)(U)C Size-exclusion chromatopraphy (IZON) DC SEC (IZON) |
Bordas, Marie | 2020 | 63% | |
Study summaryFull title
All authors
Marie Bordas, Géraldine Genard, Sibylle Ohl, Michelle Nessling, Karsten Richter, Tobias Roider, Sascha Dietrich, Kendra K Maaß, Martina Seiffert
Journal
Int J Mol Sci
Abstract
Small extracellular vesicles (sEVs) are nanoparticles responsible for cell-to-cell communication rel (show more...)
EV-METRIC
63% (25th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Solid Tissue
Sample origin
CLL
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Size-exclusion chromatopraphy (IZON) DC SEC (IZON) Protein markers
EV: Alix/ TSG101/ CD81
non-EV: ATPA5/ Calreticulin Proteomics
no
Show all info
Study aim
Function/New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Mus musculus
Sample Type
Solid Tissue
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed
No
Density cushion
Density medium
Sucrose
Other
Name other separation method
Size-exclusion chromatopraphy (IZON)
Other
Name other separation method
SEC (IZON)
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ TSG101/ CD81
Detected contaminants
Calreticulin
Not detected contaminants
ATPA5
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
145
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 3.50E+10
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV200038 | 4/4 | Homo sapiens | Blood plasma | CD31 MicroBead Kit | Prattichizzo, Francesco | 2020 | 63% | |
Study summaryFull title
All authors
Francesco Prattichizzo, Valeria De Nigris, Jacopo Sabbatinelli, Angelica Giuliani, Carlos Castaño, Marcelina Párrizas, Isabel Crespo, Annalisa Grimaldi, Nicolò Baranzini, Rosangela Spiga, Elettra Mancuso, Maria Rita Rippo, Antonio Domenico Procopio, Anna Novials, Anna Rita Bonfigli, Silvia Garavelli, Lucia La Sala, Giuseppe Matarese, Paola de Candia, Fabiola Olivieri, Antonio Ceriello
Journal
Diabetes
Abstract
Innovative biomarkers are needed to improve the management of patients with type 2 diabetes mellitus (show more...)
EV-METRIC
63% (91st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Type 2 Diabetes
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
CD31 MicroBead Kit
Protein markers
EV: TSG101/ CD63/ CD81/ CD31/ CD9/ Alix
non-EV: ApoA1 Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Other
Name other separation method
CD31 MicroBead Kit
Selected surface protein(s)
CD31
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ CD31/ CD63/ TSG101
Not detected contaminants
ApoA1
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Detected EV-associated proteins
CD31/ CD9/ CD63/ CD81
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;Microarray
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
120
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
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EV200042 | 2/2 | Mus musculus | Immortalized bone marrow-derived macrophages (iBMDMs) |
DG (d)(U)C DC Filtration |
Laura Bouchareychas | 2020 | 63% | |
Study summaryFull title
All authors
Laura Bouchareychas, Phat Duong, Sergio Covarrubias, Eric Alsop, Tuan Anh Phu, Allen Chung, Michael Gomes, David Wong, Bessie Meechoovet, Allyson Capili, Ryo Yamamoto, Hiromitsu Nakauchi, Michael T McManus, Susan Carpenter, Kendall Van Keuren-Jensen, Robert L Raffai
Journal
Cell Rep
Abstract
Developing strategies that promote the resolution of vascular inflammation and atherosclerosis remai (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
genetically modified cell line
Focus vesicles
exosome
Separation protocol
Separation protocol
DG
(d)(U)C DC Filtration Protein markers
EV: Alix/ Flotillin1
non-EV: Calnexin/ GM130 Proteomics
no
EV density (g/ml)
1.09
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
Immortalized bone marrow-derived macrophages (iBMDMs)
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
95
Cell count
3.50E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
No
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
3
Orientation
Bottom-up
Rotor type
SW 40 Ti
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
None
Filtration steps
0.22µm or 0.2µm
Density cushion
Density medium
Iodixanol
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Flotillin1/ Alix
Not detected contaminants
Calnexin/ GM130
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
Other;RNase A/T1 Mix
RNAse concentration
0.4
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
74.58
EV concentration
Yes
Particle yield
Yes, as number of particles per million cells 2.40E+09
|
||||||||
EV200037 | 1/2 | Homo sapiens | HLA-DR15+ B cells |
DG SEC (non-commercial) PEG precipitation |
Xiaogang Zhang | 2020 | 63% | |
Study summaryFull title
All authors
Xiaogang Zhang , Ellen G. F. Borg , A. Manuel Liaci , Harmjan R. Vos & Willem Stoorvogel
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EV) are membrane encapsulated nanoparticles that can function in intercellul (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Density gradient
Size-exclusion chromatography (non-commercial) PEG precipitation Protein markers
EV: CD81/ MHC2/ CD63
non-EV: None Proteomics
no
EV density (g/ml)
1.09-1.13
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HLA-DR15+ B cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Cell viability (%)
98
Separation Method
Density gradient
Type
Continuous
Lowest density fraction
0%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
4
Sample volume (mL)
2
Orientation
Bottom-up
Rotor type
SW 60 Ti
Speed (g)
200000
Duration (min)
960
Fraction volume (mL)
0.3
Fraction processing
Size-exclusion chromatography
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
0.6
Resin type
Sepharose CL-2B
Other
Name other separation method
PEG precipitation
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ MHC2/ CD81
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200037 | 2/2 | Homo sapiens | Blood plasma |
DG SEC (non-commercial) PEG precipitation |
Xiaogang Zhang | 2020 | 63% | |
Study summaryFull title
All authors
Xiaogang Zhang , Ellen G. F. Borg , A. Manuel Liaci , Harmjan R. Vos & Willem Stoorvogel
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EV) are membrane encapsulated nanoparticles that can function in intercellul (show more...)
EV-METRIC
63% (91st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Density gradient
Size-exclusion chromatography (non-commercial) PEG precipitation Protein markers
EV: CD81/ CD63/ CD9
non-EV: None Proteomics
yes
EV density (g/ml)
1.09-1.13
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Density gradient
Type
Continuous
Lowest density fraction
0%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
4
Sample volume (mL)
2
Orientation
Bottom-up
Rotor type
SW 60 Ti
Speed (g)
200000
Duration (min)
960
Fraction volume (mL)
0.3
Fraction processing
Size-exclusion chromatography
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
0.6
Resin type
Sepharose CL-2B
Other
Name other separation method
PEG precipitation
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Proteomics database
Yes:
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM/ Cryo-EM
Image type
Wide-field
|
||||||||
EV200011 | 1/1 | Bos taurus | milk |
(d)(U)C UF |
Lingjun, Tong | 2020 | 63% | |
Study summaryFull title
All authors
Lingjun Tong, Haining Hao, Xinyi Zhang, Zhe Zhang, Youyou Lv, Lanwei Zhang, Huaxi Yi
Journal
Mol Nutr Food Res
Abstract
Scope: Milk-derived extracellular vesicles (mEVs) as nanoparticles are being developed as novel drug (show more...)
EV-METRIC
63% (78th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
milk
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV: TSG101/ Alix/ CD9/ CD81
non-EV: Calnexin/ Histone H3 Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Bos taurus
Sample Type
milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Between 50,000 g and 100,000 g Pelleting performed
No
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Regenerated cellulose
Characterization: Protein analysis
PMID previous EV protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ TSG101/ Alix/ CD81
Not detected contaminants
Calnexin/ Histone H3
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
CD9
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
PMID previous EV particle analysis
Extra particle analysis
NTA
Report type
Mean
Reported size (nm)
102.8
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 1.10E+10
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV190050 | 1/1 | Mus musculus | 4T1 |
DG Filtration SEC SEC (non-commercial) |
Ger J A Arkesteijn | 2020 | 63% | |
Study summaryFull title
All authors
Ger J A Arkesteijn, Estefanía Lozano-Andrés, Sten F W M Libregts, Marca H M Wauben
Journal
Cytometry A
Abstract
Flow cytometry allows multiparameter analysis on a single-cell basis and is currently the method of (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
breast tumor model
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
Filtration SEC Size-exclusion chromatography (non-commercial) Protein markers
EV: TSG101/ CD63/ CD81/ HSP90/ Alix/ Flotillin1/ Flotillin2/ HSP70/ MHC2/ CD9/ MHC1
non-EV: Proteomics
no
EV density (g/ml)
1.10-1.12
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
4T1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
90
Separation Method
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
16.5
Sample volume (mL)
1
Orientation
Top-down
Rotor type
SW 32.1 Ti
Speed (g)
100000
Duration (min)
1091
Fraction volume (mL)
1
Fraction processing
Size-exclusion chromatography
Filtration steps
0.45µm > x > 0.22µm,
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
2
Resin type
Sepharose CL-2B
Other
Name other separation method
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63
Not detected EV-associated proteins
HSP90/ HSP70/ MHC1/ CD81/ Flotillin1/ TSG101/ MHC2/ Flotillin2/ Alix
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
139.8
EV concentration
Yes
|
||||||||
EV190036 | 1/3 | Homo sapiens | OX1-19 |
Filtration qEV |
Hicks, David | 2020 | 63% | |
Study summaryFull title
All authors
David A Hicks, Alys C Jones, Nicola J Corbett, Kate Fisher, Stuart M Pickering-Brown, Mark P Ashe, Nigel M Hooper
Journal
Neurochem Res.
Abstract
Healthy brain function is mediated by several complementary signalling pathways, many of which are d (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
qEV Protein markers
EV: TSG101/ CD9
non-EV: Mitofilin/ Grp78 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
OX1-19
EV-harvesting Medium
Serum free medium
Separation Method
Filtration steps
0.22µm or 0.2µm
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ TSG101
Not detected contaminants
Mitofilin/ Grp78
Proteomics database
No
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing
Database
No
Proteinase treatment
No
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
RNase A
RNAse concentration
0.05
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
SEC fraction-dependent
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV190036 | 2/3 | Homo sapiens | OX1-19 |
Filtration qEV |
Hicks, David | 2020 | 63% | |
Study summaryFull title
All authors
David A Hicks, Alys C Jones, Nicola J Corbett, Kate Fisher, Stuart M Pickering-Brown, Mark P Ashe, Nigel M Hooper
Journal
Neurochem Res.
Abstract
Healthy brain function is mediated by several complementary signalling pathways, many of which are d (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
qEV Protein markers
EV: TSG101/ CD9
non-EV: Mitofilin/ Grp78 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
OX1-19
EV-harvesting Medium
Serum free medium
Separation Method
Filtration steps
0.22µm or 0.2µm
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ TSG101
Not detected contaminants
Grp78/ Mitofilin
Proteomics database
No
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
RNase A
RNAse concentration
0.05
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
SEC fraction-dependent
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV190036 | 3/3 | Homo sapiens | OX1-19 |
Filtration qEV |
Hicks, David | 2020 | 63% | |
Study summaryFull title
All authors
David A Hicks, Alys C Jones, Nicola J Corbett, Kate Fisher, Stuart M Pickering-Brown, Mark P Ashe, Nigel M Hooper
Journal
Neurochem Res.
Abstract
Healthy brain function is mediated by several complementary signalling pathways, many of which are d (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
qEV Protein markers
EV: TSG101/ CD9
non-EV: Mitofilin/ Grp78 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
OX1-19
EV-harvesting Medium
Serum free medium
Separation Method
Filtration steps
0.22µm or 0.2µm
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ TSG101
Not detected contaminants
Mitofilin/ Grp78
Proteomics database
No
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
RNase A
RNAse concentration
0.05
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
SEC fraction-dependent
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV190013 | 1/1 | Homo sapiens | UW228-2 |
(d)(U)C Filtration |
Johnson, Suzanne M | 2020 | 63% | |
Study summaryFull title
All authors
Suzanne M Johnson, Antonia Banyard, Christopher Smith, Aleksandr Mironov, Martin G McCabe
Journal
Int J Mol Sci
Abstract
Extracellular vesicles (EVs) are heterogeneous in size (30 nm-10 µm), content (lipid, RNA, DNA, pro (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: CD63/ CD9/ LAMP1
non-EV: Proteomics
no
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
UW228-2
EV-harvesting Medium
Serum free medium
Cell viability (%)
NA
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
A-4-38
Pelleting: speed (g)
2000
Filtration steps
> 0.45 µm,
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
Imagestream
Antibody details provided?
No
Detected EV-associated proteins
CD63
Detected EV-associated proteins
CD63/ CD9/ LAMP1
Detected EV-associated proteins
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Size range/distribution
Reported size (nm)
600-6000
Particle analysis: flow cytometry
Flow cytometer type
Imagestream
Hardware adjustment
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
4500
|
||||||||
EV200192 | 1/2 | Exophiala dermatitidis | EXF-10123 |
(d)(U)C Filtration UF DG |
Lavrin, Teja | 2020 | 58% | |
Study summaryFull title
All authors
Teja Lavrin, Tilen Konte, Rok Kostanjšek, Simona Sitar, Kristina Sepčič, Sonja Prpar Mihevc, Ema Žagar, Vera Župunski, Metka Lenassi, Boris Rogelj, Nina Gunde Cimerman
Journal
Cells
Abstract
The neurotropic and extremophilic black yeast Exophiala dermatitidis (Herpotrichellaceae) inhabits d (show more...)
EV-METRIC
58% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Ultrafiltration Density gradient Protein markers
EV: None
non-EV: None Proteomics
no
EV density (g/ml)
1.09–1.32
Show all info
Study aim
Function
Sample
Species
Exophiala dermatitidis
Sample Type
Cell culture supernatant
EV-producing cells
EXF-10123
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
MLA-50
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
70
Wash: Rotor Type
TLA-55
Wash: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
20
Highest density fraction
60
Total gradient volume, incl. sample (mL)
4.8
Sample volume (mL)
0.4
Orientation
Not specified
Rotor type
Not specified
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
0.4
Fraction processing
Precipitation
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Not specified
Other
Name other separation method
Filtration
Other
Name other separation method
Ultrafiltration
Other
Name other separation method
Density gradient
Characterization: Protein analysis
None
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Hardware adjustment
EM
EM-type
Transmission-EM
Image type
Close-up
Report type
Mean
Report size
90
EV-concentration
Yes
|
||||||||
EV200192 | 2/2 | Exophiala dermatitidis | EXF-10123 |
(d)(U)C Filtration UF DG |
Lavrin, Teja | 2020 | 58% | |
Study summaryFull title
All authors
Teja Lavrin, Tilen Konte, Rok Kostanjšek, Simona Sitar, Kristina Sepčič, Sonja Prpar Mihevc, Ema Žagar, Vera Župunski, Metka Lenassi, Boris Rogelj, Nina Gunde Cimerman
Journal
Cells
Abstract
The neurotropic and extremophilic black yeast Exophiala dermatitidis (Herpotrichellaceae) inhabits d (show more...)
EV-METRIC
58% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Melanin inhibitor
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Ultrafiltration Density gradient Protein markers
EV: None
non-EV: None Proteomics
no
EV density (g/ml)
1.09–1.32
Show all info
Study aim
Function
Sample
Species
Exophiala dermatitidis
Sample Type
Cell culture supernatant
EV-producing cells
EXF-10123
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
MLA-50
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
70
Wash: Rotor Type
TLA-55
Wash: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
20
Highest density fraction
60
Total gradient volume, incl. sample (mL)
4.8
Sample volume (mL)
0.4
Orientation
Not specified
Rotor type
Not specified
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
0.4
Fraction processing
Precipitation
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Not specified
Other
Name other separation method
Filtration
Other
Name other separation method
Ultrafiltration
Other
Name other separation method
Density gradient
Characterization: Protein analysis
None
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Hardware adjustment
EM
EM-type
Transmission-EM
Image type
Close-up
Report type
Mean
Report size
75
EV-concentration
Yes
|
||||||||
EV200033 | 1/2 | Pseudomonas aeruginosa | PAO1 |
DG (d)(U)C UF Filtration |
Dinh, Nhung Thi Hong | 2020 | 57% | |
Study summaryFull title
All authors
Nhung Thi Hong Dinh, Jaewook Lee, Jaemin Lee, Sang Soo Kim, Gyeongyun Go, Seoyoon Bae, Ye In Jun, Yae Jin Yoon, Tae-Young Roh, Yong Song Gho
Journal
J Extracell Vesicles
Abstract
Indoor pollutants are important problems to public health. Among indoor pollutants, indoor dust cont (show more...)
EV-METRIC
57% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C UF Filtration Protein markers
EV: None
non-EV: None Proteomics
no
EV density (g/ml)
N/A
Show all info
Study aim
Function
Sample
Species
Pseudomonas aeruginosa
Sample Type
Cell culture supernatant
EV-producing cells
PAO1
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
180
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
150000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
3
Lowest density fraction
10%
Highest density fraction
50%
Total gradient volume, incl. sample (mL)
5.25
Sample volume (mL)
2.5
Orientation
Bottom-up
Rotor type
SW 55 Ti
Speed (g)
200000
Duration (min)
120
Fraction volume (mL)
0.5
Fraction processing
None
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polysulfone;Other
Characterization: Protein analysis
None
Protein Concentration Method
Bradford
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
81.9
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV190052 | 1/1 | Solanum lycopersicum | root exudate solution |
(d)(U)C Filtration |
De Palma, Monica | 2020 | 57% | |
Study summaryFull title
All authors
Monica De Palma, Alfredo Ambrosone, Antonietta Leone, Pasquale Del Gaudio, Michelina Ruocco, Lilla Turiák, Ramesh Bokka, Immacolata Fiume, Marina Tucci, Gabriella Pocsfalvi
Journal
Plants (Basel)
Abstract
Extracellular Vesicles (EVs) play pivotal roles in cell-to-cell and inter-kingdom communication. Des (show more...)
EV-METRIC
57% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
root exudate solution
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Solanum lycopersicum
Sample Type
root exudate solution
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
150000
Wash: volume per pellet (ml)
28.5
Wash: time (min)
60
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
150000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
microBCA
Proteomics database
Yes:
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
72 +/- 5 nm
TRPS
Report type
Size range/distribution
Reported size (nm)
51
EV concentration
Yes
EM
EM-type
Scanning electron microscopy
Image type
Close-up, Wide-field
Report size (nm)
50-100
|
||||||||
EV190032 | 1/2 | Schistosoma mansoni | NA | (d)(U)C | Marije E Kuipers | 2020 | 57% | |
Study summaryFull title
All authors
Marije E Kuipers, Esther N M Nolte-'t Hoen, Alwin J van der Ham, Arifa Ozir-Fazalalikhan, D Linh Nguyen, Clarize M de Korne, Roman I Koning, John J Tomes, Karl F Hoffmann, Hermelijn H Smits, Cornelis H Hokke
Journal
J Extracell Vesicles
Abstract
Helminths like Schistosoma mansoni release excretory/secretory (E/S) products that modulate host imm (show more...)
EV-METRIC
57% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Schisostomula (larval stage) culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
213.2 (pelleting) / 213.2 (washing)
Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Mechanism of uptake/transfer, Identification of content (omics approaches)
Sample
Species
Schistosoma mansoni
Sample Type
Schisostomula (larval stage) culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
80
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
120000
Pelleting: adjusted k-factor
213.2
Wash: time (min)
60
Wash: Rotor Type
SW 41 Ti
Wash: speed (g)
120000
Wash: adjusted k-factor
213.2
Characterization: Protein analysis
PMID previous EV protein analysis
26443722
Protein Concentration Method
microBCA
Protein Yield (µg)
6
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
30-650
EV concentration
Yes
Particle yield
23300000000
EM
EM-type
Transmission-EM/ Cryo-EM
Image type
Close-up, Wide-field
Report size (nm)
35-190;30-715
|
||||||||
EV190032 | 2/2 | Schistosoma mansoni | NA | (d)(U)C | Marije E Kuipers | 2020 | 57% | |
Study summaryFull title
All authors
Marije E Kuipers, Esther N M Nolte-'t Hoen, Alwin J van der Ham, Arifa Ozir-Fazalalikhan, D Linh Nguyen, Clarize M de Korne, Roman I Koning, John J Tomes, Karl F Hoffmann, Hermelijn H Smits, Cornelis H Hokke
Journal
J Extracell Vesicles
Abstract
Helminths like Schistosoma mansoni release excretory/secretory (E/S) products that modulate host imm (show more...)
EV-METRIC
57% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Schisostomula (larval stage) culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
213.2 (pelleting) / 83.21 (washing)
Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Mechanism of uptake/transfer, Identification of content (omics approaches)
Sample
Species
Schistosoma mansoni
Sample Type
Schisostomula (larval stage) culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
120000
Pelleting: adjusted k-factor
213.2
Wash: time (min)
65
Wash: Rotor Type
TLS-55
Wash: speed (g)
120000
Wash: adjusted k-factor
83.21
Characterization: Protein analysis
PMID previous EV protein analysis
26443722
Protein Concentration Method
microBCA
Protein Yield (µg)
6
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
30-650
EV concentration
Yes
Particle yield
23300000000
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
35-190
|
||||||||
EV220160 | 1/2 | Homo sapiens | MLO-Y4 |
(d)(U)C Filtration |
Eichholz KF | 2020 | 56% | |
Study summaryFull title
All authors
Eichholz KF, Woods I, Riffault M, Johnson GP, Corrigan M, Lowry MC, Shen N, Labour MN, Wynne K, O'Driscoll L, Hoey DA
Journal
Stem Cells Transl Med
Abstract
Bone formation or regeneration requires the recruitment, proliferation, and osteogenic differentiati (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Statically cultured
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: TSG101/ Alix
non-EV: GRP-94 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MLO-Y4
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
not reported
Wash: time (min)
75
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
TSG101/ Alix
Not detected contaminants
GRP-94
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
177
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200182 | 4/11 | Mus musculus | primary oligodendrocytes |
(d)(U)C DC DG |
Frühbeis, Carsten | 2020 | 56% | |
Study summaryFull title
All authors
Carsten Frühbeis, Wen Ping Kuo-Elsner, Christina Müller, Kerstin Barth, Leticia Peris, Stefan Tenzer, Wiebke Möbius, Hauke B Werner, Klaus-Armin Nave, Dominik Fröhlich, Eva-Maria Krämer-Albers
Journal
PLoS Biol
Abstract
Neurons extend long axons that require maintenance and are susceptible to degeneration. Long-term in (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
PLPko
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Density gradient Protein markers
EV: SIRT2/ Flotillin1
non-EV: None Proteomics
yes
EV density (g/ml)
1.07-1.1
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
primary oligodendrocytes
EV-harvesting Medium
Serum free medium
Cell count
3.00E+06
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW 40 Ti
Pelleting: speed (g)
100000
Density gradient
Type
Continuous
Lowest density fraction
0.3M
Highest density fraction
1.8M
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
1
Orientation
Top-down
Rotor type
SW 40 Ti
Speed (g)
100000
Duration (min)
960
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
1
Pelleting: duration (min)
60
Pelleting: rotor type
TLS-55
Pelleting: speed (g)
100000
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ SIRT2
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV200182 | 7/11 | Mus musculus | primary oligodendrocytes |
(d)(U)C DC DG |
Frühbeis, Carsten | 2020 | 56% | |
Study summaryFull title
All authors
Carsten Frühbeis, Wen Ping Kuo-Elsner, Christina Müller, Kerstin Barth, Leticia Peris, Stefan Tenzer, Wiebke Möbius, Hauke B Werner, Klaus-Armin Nave, Dominik Fröhlich, Eva-Maria Krämer-Albers
Journal
PLoS Biol
Abstract
Neurons extend long axons that require maintenance and are susceptible to degeneration. Long-term in (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
CNPko
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Density gradient Protein markers
EV: SIRT2/ Flotillin1/ PLP
non-EV: None Proteomics
yes
EV density (g/ml)
1.07-1,1
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
primary oligodendrocytes
EV-harvesting Medium
Serum free medium
Cell count
3.00E+06
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW 40 Ti
Pelleting: speed (g)
100000
Density gradient
Type
Continuous
Lowest density fraction
0.3M
Highest density fraction
1.8M
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
1ml
Orientation
Top-down
Rotor type
SW 40 Ti
Speed (g)
100000
Duration (min)
960
Fraction volume (mL)
1ml
Fraction processing
Centrifugation
Pelleting: volume per fraction
1ml
Pelleting: duration (min)
60
Pelleting: rotor type
TLS-55
Pelleting: speed (g)
100000
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ PLP/ SIRT2
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV200171 | 1/8 | Homo sapiens | primary adipose tissue-derived mesenchymal stromal cells | (d)(U)C | Gorgun, Cansu | 2020 | 56% | |
Study summaryFull title
All authors
Cansu Gorgun, Davide Ceresa, Raphaelle Lesage, Federico Villa, Daniele Reverberi, Carolina Balbi, Sara Santamaria, Katia Cortese, Paolo Malatesta, Liesbet Geris, Rodolfo Quarto, Roberta Tasso
Journal
Biomaterials
Abstract
Mesenchymal stromal cells (MSCs) are characterized by a regulatory phenotype and respond promptly to (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Mitofilin/ CD63/ CD9/ Syntenin-1
non-EV: GRP94/ Lamin A Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary adipose tissue-derived mesenchymal stromal cells
EV-harvesting Medium
Serum free medium
Cell viability (%)
70
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
40
Pelleting: rotor type
SW 28
Pelleting: speed (g)
10000
Wash: volume per pellet (ml)
5
Wash: time (min)
40
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
10000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Syntenin-1
Not detected EV-associated proteins
Mitofilin
Not detected contaminants
GRP94/ Lamin A
Flow cytometry
Type of Flow cytometry
BD FACSAria II
Hardware adaptation to ~100nm EV's
Gorgun, Cansu, et al. ""Isolation and Flow Cytometry Characterization of ExtracellularVesicle Subpopulations Derived from Human Mesenchymal Stromal Cells."" Current protocols in stem cell biology 48.1 (2019): e76.
Calibration bead size
100,160,200,240,300,500,900
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD9/ CD81
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
232
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 2.19E+07
Particle analysis: flow cytometry
Flow cytometer type
BD FACSAria II
Hardware adjustment
Gorgun, Cansu, et al. ""Isolation and Flow Cytometry Characterization of ExtracellularVesicle Subpopulations Derived from Human Mesenchymal Stromal Cells."" Current protocols in stem cell biology 48.1 (2019): e76.
Calibration bead size
100,160,200,240,300,500,900
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200171 | 2/8 | Homo sapiens | primary adipose tissue-derived mesenchymal stromal cells | (d)(U)C | Gorgun, Cansu | 2020 | 56% | |
Study summaryFull title
All authors
Cansu Gorgun, Davide Ceresa, Raphaelle Lesage, Federico Villa, Daniele Reverberi, Carolina Balbi, Sara Santamaria, Katia Cortese, Paolo Malatesta, Liesbet Geris, Rodolfo Quarto, Roberta Tasso
Journal
Biomaterials
Abstract
Mesenchymal stromal cells (MSCs) are characterized by a regulatory phenotype and respond promptly to (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Mitofilin/ CD63/ CD9/ Syntenin-1
non-EV: GRP94/ Lamin A Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary adipose tissue-derived mesenchymal stromal cells
EV-harvesting Medium
Serum free medium
Cell viability (%)
70
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
5
Wash: time (min)
120
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Syntenin-1
Not detected EV-associated proteins
Mitofilin
Not detected contaminants
GRP94/ Lamin A
Flow cytometry
Type of Flow cytometry
BD FACSAria II
Hardware adaptation to ~100nm EV's
Gorgun, Cansu, et al. ""Isolation and Flow Cytometry Characterization of ExtracellularVesicle Subpopulations Derived from Human Mesenchymal Stromal Cells."" Current protocols in stem cell biology 48.1 (2019): e76.
Calibration bead size
100,160,200,240,300,500,900
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD9/ CD81
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
164
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 2.39E+07
Particle analysis: flow cytometry
Flow cytometer type
BD FACSAria II
Hardware adjustment
Gorgun, Cansu, et al. ""Isolation and Flow Cytometry Characterization of ExtracellularVesicle Subpopulations Derived from Human Mesenchymal Stromal Cells."" Current protocols in stem cell biology 48.1 (2019): e76.
Calibration bead size
100,160,200,240,300,500,900
Report type
Size range/distribution
Reported size (nm)
Smaller than 160 nm
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200171 | 3/8 | Homo sapiens | primary adipose tissue-derived mesenchymal stromal cells |
(d)(U)C |
Gorgun, Cansu | 2020 | 56% | |
Study summaryFull title
All authors
Cansu Gorgun, Davide Ceresa, Raphaelle Lesage, Federico Villa, Daniele Reverberi, Carolina Balbi, Sara Santamaria, Katia Cortese, Paolo Malatesta, Liesbet Geris, Rodolfo Quarto, Roberta Tasso
Journal
Biomaterials
Abstract
Mesenchymal stromal cells (MSCs) are characterized by a regulatory phenotype and respond promptly to (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Hypoxia
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
No extra separation steps Protein markers
EV: CD81/ Mitofilin/ CD63/ CD9/ Syntenin-1
non-EV: GRP94/ Lamin A Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary adipose tissue-derived mesenchymal stromal cells
EV-harvesting Medium
Serum free medium
Cell viability (%)
70
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
5
Wash: time (min)
120
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Other
Name other separation method
No extra separation steps
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Syntenin-1
Not detected EV-associated proteins
Mitofilin
Detected contaminants
Lamin A
Not detected contaminants
GRP94
Flow cytometry
Hardware adaptation to ~100nm EV's
Gorgun, Cansu, et al. ""Isolation and Flow Cytometry Characterization of ExtracellularVesicle Subpopulations Derived from Human Mesenchymal Stromal Cells."" Current protocols in stem cell biology 48.1 (2019): e76.
Calibration bead size
100,160,200,240,300,500,900
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD9/ CD81
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
164
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 7.06E+07
Particle analysis: flow cytometry
Flow cytometer type
BD FACSAria II
Hardware adjustment
Gorgun, Cansu, et al. ""Isolation and Flow Cytometry Characterization of ExtracellularVesicle Subpopulations Derived from Human Mesenchymal Stromal Cells."" Current protocols in stem cell biology 48.1 (2019): e76.
Calibration bead size
100,160,200,240,300,500,900
Report type
Size range/distribution
Reported size (nm)
Smaller than 160 nm
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200171 | 4/8 | Homo sapiens | primary adipose tissue-derived mesenchymal stromal cells | (d)(U)C | Gorgun, Cansu | 2020 | 56% | |
Study summaryFull title
All authors
Cansu Gorgun, Davide Ceresa, Raphaelle Lesage, Federico Villa, Daniele Reverberi, Carolina Balbi, Sara Santamaria, Katia Cortese, Paolo Malatesta, Liesbet Geris, Rodolfo Quarto, Roberta Tasso
Journal
Biomaterials
Abstract
Mesenchymal stromal cells (MSCs) are characterized by a regulatory phenotype and respond promptly to (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Hypoxia
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Mitofilin/ CD63/ CD9/ Syntenin-1
non-EV: GRP94/ Lamin A Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary adipose tissue-derived mesenchymal stromal cells
EV-harvesting Medium
Serum free medium
Cell viability (%)
70
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
40
Pelleting: rotor type
SW 28
Pelleting: speed (g)
10000
Wash: volume per pellet (ml)
5
Wash: time (min)
40
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
10000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Syntenin-1
Not detected EV-associated proteins
Mitofilin
Not detected contaminants
GRP94/ Lamin A
Flow cytometry
Type of Flow cytometry
BD FACSAria II
Hardware adaptation to ~100nm EV's
Gorgun, Cansu, et al. ""Isolation and Flow Cytometry Characterization of ExtracellularVesicle Subpopulations Derived from Human Mesenchymal Stromal Cells."" Current protocols in stem cell biology 48.1 (2019): e76.
Calibration bead size
100,160,200,240,300,500,900
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD9/ CD81
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
164
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 4.54E+07
Particle analysis: flow cytometry
Flow cytometer type
BD FACSAria II
Hardware adjustment
Gorgun, Cansu, et al. ""Isolation and Flow Cytometry Characterization of ExtracellularVesicle Subpopulations Derived from Human Mesenchymal Stromal Cells."" Current protocols in stem cell biology 48.1 (2019): e76.
Calibration bead size
100,160,200,240,300,500,900
Report type
Size range/distribution
Reported size (nm)
Smaller than 160 nm
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200171 | 5/8 | Homo sapiens | primary adipose tissue-derived mesenchymal stromal cells | (d)(U)C | Gorgun, Cansu | 2020 | 56% | |
Study summaryFull title
All authors
Cansu Gorgun, Davide Ceresa, Raphaelle Lesage, Federico Villa, Daniele Reverberi, Carolina Balbi, Sara Santamaria, Katia Cortese, Paolo Malatesta, Liesbet Geris, Rodolfo Quarto, Roberta Tasso
Journal
Biomaterials
Abstract
Mesenchymal stromal cells (MSCs) are characterized by a regulatory phenotype and respond promptly to (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Normoxia + IL1Alpha + TNFAlpha
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Mitofilin/ CD63/ CD9/ Syntenin-1
non-EV: GRP94/ Lamin A Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary adipose tissue-derived mesenchymal stromal cells
EV-harvesting Medium
Serum free medium
Cell viability (%)
70
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
5
Wash: time (min)
120
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Syntenin-1
Not detected EV-associated proteins
Mitofilin
Not detected contaminants
GRP94/ Lamin A
Flow cytometry
Type of Flow cytometry
BD FACSAria II
Hardware adaptation to ~100nm EV's
Gorgun, Cansu, et al. ""Isolation and Flow Cytometry Characterization of ExtracellularVesicle Subpopulations Derived from Human Mesenchymal Stromal Cells."" Current protocols in stem cell biology 48.1 (2019): e76.
Calibration bead size
100,160,200,240,300,500,900
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD9/ CD81
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
232
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 2.57E+07
Particle analysis: flow cytometry
Flow cytometer type
BD FACSAria II
Hardware adjustment
Gorgun, Cansu, et al. ""Isolation and Flow Cytometry Characterization of ExtracellularVesicle Subpopulations Derived from Human Mesenchymal Stromal Cells."" Current protocols in stem cell biology 48.1 (2019): e76.
Calibration bead size
100,160,200,240,300,500,900
Report type
Size range/distribution
Reported size (nm)
Bigger than 160 nm
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200171 | 7/8 | Homo sapiens | primary adipose tissue-derived mesenchymal stromal cells | (d)(U)C | Gorgun, Cansu | 2020 | 56% | |
Study summaryFull title
All authors
Cansu Gorgun, Davide Ceresa, Raphaelle Lesage, Federico Villa, Daniele Reverberi, Carolina Balbi, Sara Santamaria, Katia Cortese, Paolo Malatesta, Liesbet Geris, Rodolfo Quarto, Roberta Tasso
Journal
Biomaterials
Abstract
Mesenchymal stromal cells (MSCs) are characterized by a regulatory phenotype and respond promptly to (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Hypoxia + IL1Alpha + TNFAlpha
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Mitofilin/ CD63/ CD9/ Syntenin-1
non-EV: GRP94/ Lamin A Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary adipose tissue-derived mesenchymal stromal cells
EV-harvesting Medium
Serum free medium
Cell viability (%)
70
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
5
Wash: time (min)
120
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Syntenin-1
Not detected EV-associated proteins
Mitofilin
Not detected contaminants
GRP94/ Lamin A
Flow cytometry
Type of Flow cytometry
BD FACSAria II
Hardware adaptation to ~100nm EV's
Gorgun, Cansu, et al. ""Isolation and Flow Cytometry Characterization of ExtracellularVesicle Subpopulations Derived from Human Mesenchymal Stromal Cells."" Current protocols in stem cell biology 48.1 (2019): e76.
Calibration bead size
100,160,200,240,300,500,900
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD9/ CD81
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
164
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 6.23E+07
Particle analysis: flow cytometry
Flow cytometer type
BD FACSAria II
Hardware adjustment
Gorgun, Cansu, et al. ""Isolation and Flow Cytometry Characterization of ExtracellularVesicle Subpopulations Derived from Human Mesenchymal Stromal Cells."" Current protocols in stem cell biology 48.1 (2019): e76.
Calibration bead size
100,160,200,240,300,500,900
Report type
Size range/distribution
Reported size (nm)
Smaller than 160 nm
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200171 | 8/8 | Homo sapiens | primary adipose tissue-derived mesenchymal stromal cells | (d)(U)C | Gorgun, Cansu | 2020 | 56% | |
Study summaryFull title
All authors
Cansu Gorgun, Davide Ceresa, Raphaelle Lesage, Federico Villa, Daniele Reverberi, Carolina Balbi, Sara Santamaria, Katia Cortese, Paolo Malatesta, Liesbet Geris, Rodolfo Quarto, Roberta Tasso
Journal
Biomaterials
Abstract
Mesenchymal stromal cells (MSCs) are characterized by a regulatory phenotype and respond promptly to (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Hypoxia + IL1Alpha + TNFAlpha
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Mitofilin/ CD63/ CD9/ Syntenin-1
non-EV: GRP94/ Lamin A Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary adipose tissue-derived mesenchymal stromal cells
EV-harvesting Medium
Serum free medium
Cell viability (%)
70
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
40
Pelleting: rotor type
SW 28
Pelleting: speed (g)
10000
Wash: volume per pellet (ml)
5
Wash: time (min)
40
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
10000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Syntenin-1
Not detected EV-associated proteins
Mitofilin
Not detected contaminants
GRP94/ Lamin A
Flow cytometry
Type of Flow cytometry
BD FACSAria II
Hardware adaptation to ~100nm EV's
Gorgun, Cansu, et al. ""Isolation and Flow Cytometry Characterization of ExtracellularVesicle Subpopulations Derived from Human Mesenchymal Stromal Cells."" Current protocols in stem cell biology 48.1 (2019): e76.
Calibration bead size
100,160,200,240,300,500,900
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD9/ CD81
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
232
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 2.91E+07
Particle analysis: flow cytometry
Flow cytometer type
BD FACSAria II
Hardware adjustment
Gorgun, Cansu, et al. ""Isolation and Flow Cytometry Characterization of ExtracellularVesicle Subpopulations Derived from Human Mesenchymal Stromal Cells."" Current protocols in stem cell biology 48.1 (2019): e76.
Calibration bead size
100,160,200,240,300,500,900
Report type
Size range/distribution
Reported size (nm)
Bigger than 160 nm
EM
EM-type
Transmission-EM
Image type
Wide-field
|
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