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You searched for: 2019 (Year of publication)
Showing 151 - 200 of 963
Showing 151 - 200 of 963
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV220279 | 1/5 | Homo sapiens | Umbilical cord blood-mesenchymal stem cells | (d)(U)C | Sung DK | 2019 | 44% | |
Study summaryFull title
All authors
Sung DK, Chang YS, Sung SI, Ahn SY, Park WS
Journal
J Clin Med
Abstract
The aim of this study was to determine the optimal preconditioning regimen for the wound healing the (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ CD63/ CD9/ Angiogenin/ Angiopoietin-1/ HGF/ IGFBP-2/ IGFBP-3/ Pentraxin-3/ Serpin E1/ TIMP-1/ Thrombospondin-1/ uPA/ VEGF
non-EV: Cytochrome C/ Fibrillarin/ GM130 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Umbilical cord blood-mesenchymal stem cells
EV-harvesting Medium
Serum free medium
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000rp
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Not detected contaminants
Fibrillarin/ Cytochrome C/ GM130
ELISA
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
Angiogenin/ Angiopoietin-1/ HGF/ VEGF
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
30-100
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM/ Scanning-EM
Image type
Close-up
|
||||||||
EV220279 | 2/5 | Homo sapiens | Umbilical cord blood-mesenchymal stem cells | (d)(U)C | Sung DK | 2019 | 44% | |
Study summaryFull title
All authors
Sung DK, Chang YS, Sung SI, Ahn SY, Park WS
Journal
J Clin Med
Abstract
The aim of this study was to determine the optimal preconditioning regimen for the wound healing the (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Thrombin
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ CD63/ CD9/ Angiogenin/ Angiopoietin-1/ HGF/ IGFBP-2/ IGFBP-3/ Pentraxin-3/ Serpin E1/ TIMP-1/ Thrombospondin-1/ uPA/ VEGF
non-EV: Cytochrome C/ Fibrillarin/ GM130 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Umbilical cord blood-mesenchymal stem cells
EV-harvesting Medium
Serum free medium
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000rp
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Not detected contaminants
Fibrillarin/ Cytochrome C/ GM130
ELISA
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
Angiogenin/ Angiopoietin-1/ HGF/ VEGF
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
30-100
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM/ Scanning-EM
Image type
Close-up
|
||||||||
EV220279 | 3/5 | Homo sapiens | Umbilical cord blood-mesenchymal stem cells | (d)(U)C | Sung DK | 2019 | 44% | |
Study summaryFull title
All authors
Sung DK, Chang YS, Sung SI, Ahn SY, Park WS
Journal
J Clin Med
Abstract
The aim of this study was to determine the optimal preconditioning regimen for the wound healing the (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
LPS
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ CD63/ CD9/ Angiogenin/ Angiopoietin-1/ HGF/ IGFBP-2/ IGFBP-3/ Pentraxin-3/ Serpin E1/ TIMP-1/ Thrombospondin-1/ uPA/ VEGF
non-EV: Cytochrome C/ Fibrillarin/ GM130 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Umbilical cord blood-mesenchymal stem cells
EV-harvesting Medium
Serum free medium
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000rp
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Not detected contaminants
Fibrillarin/ Cytochrome C/ GM130
ELISA
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
Angiogenin/ Angiopoietin-1/ HGF/ VEGF
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
30-100
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM/ Scanning-EM
Image type
Close-up
|
||||||||
EV220279 | 4/5 | Homo sapiens | Umbilical cord blood-mesenchymal stem cells | (d)(U)C | Sung DK | 2019 | 44% | |
Study summaryFull title
All authors
Sung DK, Chang YS, Sung SI, Ahn SY, Park WS
Journal
J Clin Med
Abstract
The aim of this study was to determine the optimal preconditioning regimen for the wound healing the (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
H2O2
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ CD63/ CD9/ Angiogenin/ Angiopoietin-1/ HGF/ IGFBP-2/ IGFBP-3/ Pentraxin-3/ Serpin E1/ TIMP-1/ Thrombospondin-1/ uPA/ VEGF
non-EV: Cytochrome C/ Fibrillarin/ GM130 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Umbilical cord blood-mesenchymal stem cells
EV-harvesting Medium
Serum free medium
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000rp
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Detected contaminants
Cytochrome C
Not detected contaminants
Fibrillarin/ GM130
ELISA
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
Angiogenin/ Angiopoietin-1/ HGF/ VEGF
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
30-100
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM/ Scanning-EM
Image type
Close-up
|
||||||||
EV220279 | 5/5 | Homo sapiens | Umbilical cord blood-mesenchymal stem cells | (d)(U)C | Sung DK | 2019 | 44% | |
Study summaryFull title
All authors
Sung DK, Chang YS, Sung SI, Ahn SY, Park WS
Journal
J Clin Med
Abstract
The aim of this study was to determine the optimal preconditioning regimen for the wound healing the (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Hypoxia
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ CD63/ CD9/ Angiogenin/ Angiopoietin-1/ HGF/ IGFBP-2/ IGFBP-3/ Pentraxin-3/ Serpin E1/ TIMP-1/ Thrombospondin-1/ uPA/ VEGF
non-EV: Cytochrome C/ Fibrillarin/ GM130 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Umbilical cord blood-mesenchymal stem cells
EV-harvesting Medium
Serum free medium
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000rp
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Detected contaminants
Cytochrome C
Not detected contaminants
Fibrillarin/ GM130
ELISA
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
Angiogenin/ Angiopoietin-1/ HGF/ VEGF
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
30-100
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM/ Scanning-EM
Image type
Close-up
|
||||||||
EV220196 | 1/4 | Bos taurus | BL20 (immortalised bovine lymphosarcoma) | (d)(U)C | Gillan V | 2019 | 44% | |
Study summaryFull title
All authors
Gillan V, Simpson DM, Kinnaird J, Maitland K, Shiels B, Devaney E
Journal
Cell Microbiol
Abstract
The protozoan parasites Theileria annulata and Theileria parva are unique amongst intracellular euka (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Rab-5B/ CD63
non-EV: Cyc1 Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Bos taurus
Sample Type
Cell culture supernatant
EV-producing cells
BL20 (immortalised bovine lymphosarcoma)
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Surespin 630 (17 ml)
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
11
Wash: time (min)
70
Wash: Rotor Type
Surespin 630 (17 ml)
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ Rab-5B
Not detected contaminants
Cyc1
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR/ RNA sequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
100
Other particle analysis name(1)
No
|
||||||||
EV220196 | 3/4 | Bos taurus | BL20 (immortalised bovine lymphosarcoma) | (d)(U)C | Gillan V | 2019 | 44% | |
Study summaryFull title
All authors
Gillan V, Simpson DM, Kinnaird J, Maitland K, Shiels B, Devaney E
Journal
Cell Microbiol
Abstract
The protozoan parasites Theileria annulata and Theileria parva are unique amongst intracellular euka (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Theileria annulata infected (TBL20)
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Rab-5B/ CD63
non-EV: Cyc1 Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Bos taurus
Sample Type
Cell culture supernatant
EV-producing cells
BL20 (immortalised bovine lymphosarcoma)
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Surespin 630 (17 ml)
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
11
Wash: time (min)
70
Wash: Rotor Type
Surespin 630 (17 ml)
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ Rab-5B
Not detected contaminants
Cyc1
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR/ RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
100
Other particle analysis name(1)
No
|
||||||||
EV210038 | 1/1 | Homo sapiens | NCI-H460 |
(d)(U)C Filtration |
Wang, Shuang | 2019 | 44% | |
Study summaryFull title
All authors
Shuang Wang, Piaoyang Gao, Na Li, Ping Chen, Jinhan Wang, Ningning He, Kaihua Ji, Liqing Du, Qiang Liu
Journal
Int J Oncol
Abstract
Radiotherapy resistance in patient with non‑small cell lung cancer (NSCLC) reduces patient surviva (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: TSG101/ B-actin/ CD9/ B-tubulin
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
NCI-H460
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
120
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TSG101
Not detected EV-associated proteins
B-actin/ B-tubulin/ CD9
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200148 | 1/4 | Homo sapiens | Blood plasma |
"(d)(U)C Filtration SEC (non-commercial) UF" |
Menon, Ramkumar | 2019 | 44% | |
Study summaryFull title
All authors
Ramkumar Menon, Christopher Luke Dixon, Samantha Sheller-Miller, Stephen J Fortunato, George R Saade, Carlos Palma, Andrew Lai, Dominic Guanzon, Carlos Salomon
Journal
Endocrinology
Abstract
Exosomes are membrane-bound nanovesicles that transport molecular signals between cells. This study (show more...)
EV-METRIC
44% (77th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
"Pregnant; Term, not in labor"
Focus vesicles
exosome
Separation protocol
Separation protocol
"(Differential) (ultra)centrifugation
Filtration Size exclusion chromatography (non-commercial) Ultrafiltration" Protein markers
EV: "TSG101/ PLAP/ CD63/ CD9"
non-EV: Grp94 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
T-8100
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
10
Wash: time (min)
120
Wash: Rotor Type
T-8100
Wash: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Not specified
Size-exclusion chromatography
Total column volume (mL)
Not specified
Sample volume/column (mL)
0.5
Resin type
Sepharose CL-2B
Other
Name other separation method
"(Differential) (ultra)centrifugation
Other
Name other separation method
Size exclusion chromatography (non-commercial)
Other
Name other separation method
Ultrafiltration"
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Detected EV-associated proteins
"CD9/ CD63/ TSG101"
Not detected contaminants
Grp94
Proteomics database
No
Fluorescent NTA
Antibody details provided?
No
Detected EV-associated proteins
"PLAP/ CD63"
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
134
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV190092 | 2/2 | Homo sapiens | HT1080 | (d)(U)C | Brassart B | 2019 | 44% | |
Study summaryFull title
All authors
Brassart B, Da Silva J, Donet M, Seurat E, Hague F, Terryn C, Velard F, Michel J, Ouadid-Ahidouch H, Monboisse JC, Hinek A, Maquart FX, Ramont L, Brassart-Pasco S.
Journal
Br J Cancer
Abstract
BACKGROUND:
Carcinogenesis occurs in elastin-rich tissues and leads to local inflammation and elasto (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
elastin degradation product-stimulated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD9/ CD81/ RhoA/ HSP90/ Actin/ Integrin-alphaV/ P-ERM/ MMP-2/ MMP-14
non-EV: GM130 Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HT1080
EV-harvesting Medium
Serum free medium
Cell viability (%)
94
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
JLA110
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
Wash: time (min)
Wash: Rotor Type
Wash: speed (g)
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD81/ RhoA/ HSP90/ Actin/ Integrin-alphaV/ P-ERM/ MMP-2/ MMP-14
Not detected contaminants
GM130
Fluorescent NTA
Relevant measurements variables specified?
NA
Antibody details provided?
No
Detected EV-associated proteins
Not detected contaminants
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
EV concentration
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV190019 | 1/4 | Mus musculus | C2C12 |
DG (d)(U)C |
Leermakers PA | 2019 | 44% | |
Study summaryFull title
All authors
Leermakers PA, Remels AHV, Zonneveld MI, Rouschop KMA, Schols AMWJ, Gosker HR.
Journal
FASEB J
Abstract
Iron homeostasis is essential for mitochondrial function, and iron deficiency has been associated wi (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: HSP70/ Flotillin1
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.19 g/ml
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)/Mechanism of uptake/transfer
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
C2C12
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Density gradient
Type
Continuous
Lowest density fraction
0.4M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
12.4
Sample volume (mL)
0.2
Orientation
Bottom-up
Rotor type
SW 41 Ti
Speed (g)
188000
Duration (min)
16
Fraction volume (mL)
1 ml
Fraction processing
Centrifugation
Pelleting: volume per fraction
6 ml
Pelleting: duration (min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
EV-subtype
Distinction between multiple subtypes
sedimentation speed;Other
Used subtypes
16,000g
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ HSP70
Characterization: Lipid analysis
No
EM
EM-type
Cryo-EM
Image type
Wide-field
|
||||||||
EV190019 | 2/4 | Mus musculus | C2C12 |
DG (d)(U)C |
Leermakers PA | 2019 | 44% | |
Study summaryFull title
All authors
Leermakers PA, Remels AHV, Zonneveld MI, Rouschop KMA, Schols AMWJ, Gosker HR.
Journal
FASEB J
Abstract
Iron homeostasis is essential for mitochondrial function, and iron deficiency has been associated wi (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: HSP70/ Flotillin1
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.19 g/ml
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)/Mechanism of uptake/transfer
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
C2C12
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Density gradient
Type
Continuous
Lowest density fraction
0.4M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
12.4
Sample volume (mL)
0.2
Orientation
Bottom-up
Rotor type
SW 41 Ti
Speed (g)
188000
Duration (min)
16
Fraction volume (mL)
1 ml
Fraction processing
Centrifugation
Pelleting: volume per fraction
6 ml
Pelleting: duration (min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
EV-subtype
Distinction between multiple subtypes
sedimentation speed;Other
Used subtypes
100,000g
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ HSP70
Characterization: Lipid analysis
No
EM
EM-type
Cryo-EM
Image type
Wide-field
|
||||||||
EV190019 | 3/4 | Mus musculus | C2C12 |
DG (d)(U)C |
Leermakers PA | 2019 | 44% | |
Study summaryFull title
All authors
Leermakers PA, Remels AHV, Zonneveld MI, Rouschop KMA, Schols AMWJ, Gosker HR.
Journal
FASEB J
Abstract
Iron homeostasis is essential for mitochondrial function, and iron deficiency has been associated wi (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Deferiprone/iron chelation
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: HSP70/ Flotillin1
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.19 g/ml
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)/Mechanism of uptake/transfer
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
C2C12
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Density gradient
Type
Continuous
Lowest density fraction
0.4M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
12.4
Sample volume (mL)
0.2
Orientation
Bottom-up
Rotor type
SW 41 Ti
Speed (g)
188000
Duration (min)
16
Fraction volume (mL)
1 ml
Fraction processing
Centrifugation
Pelleting: volume per fraction
6 ml
Pelleting: duration (min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
EV-subtype
Distinction between multiple subtypes
sedimentation speed;Other
Used subtypes
16,000g
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ HSP70
Characterization: Lipid analysis
No
EM
EM-type
Cryo-EM
Image type
Wide-field
|
||||||||
EV190019 | 4/4 | Mus musculus | C2C12 |
DG (d)(U)C |
Leermakers PA | 2019 | 44% | |
Study summaryFull title
All authors
Leermakers PA, Remels AHV, Zonneveld MI, Rouschop KMA, Schols AMWJ, Gosker HR.
Journal
FASEB J
Abstract
Iron homeostasis is essential for mitochondrial function, and iron deficiency has been associated wi (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Deferiprone/iron chelation
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: HSP70/ Flotillin1
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.19 g/ml
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)/Mechanism of uptake/transfer
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
C2C12
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Density gradient
Type
Continuous
Lowest density fraction
0.4M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
12.4
Sample volume (mL)
0.2
Orientation
Bottom-up
Rotor type
SW 41 Ti
Speed (g)
188000
Duration (min)
16
Fraction volume (mL)
1 ml
Fraction processing
Centrifugation
Pelleting: volume per fraction
6 ml
Pelleting: duration (min)
65
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
EV-subtype
Distinction between multiple subtypes
sedimentation speed;Other
Used subtypes
100,000g
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ HSP70
Characterization: Lipid analysis
No
EM
EM-type
Cryo-EM
Image type
Wide-field
|
||||||||
EV190016 | 1/1 | Homo sapiens | Wharton's jelly mesenchymal stromal cells | (d)(U)C | Thomi, Gierin | 2019 | 44% | |
Study summaryFull title
All authors
Gierin Thomi, Daniel Surbek, Valérie Haesler, Marianne Joerger-Messerli, Andreina Schoeberlein
Journal
Stem Cell Res Ther
Abstract
Background: Preterm newborns are at high risk of developing neurodevelopmental deficits caused by ne (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ CD63/ CD81/ Alix/ ICAM/ Flotillin1/ EpCAM/ ANXA5
non-EV: GRP94/ GM130 Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Wharton's jelly mesenchymal stromal cells
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
TFT-70
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
5
Wash: time (min)
70
Wash: Rotor Type
TFT-70
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Antibody details provided?
No
Not detected contaminants
GRP94
Detected EV-associated proteins
Alix/ Flotillin1/ CD63/ EpCAM/ ICAM/ ANXA5/ CD81/ TSG101
Not detected contaminants
GM130
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
42.93
|
||||||||
EV190011 | 2/5 | Mus musculus | E0771 | (d)(U)C | Cianciaruso C | 2019 | 44% | |
Study summaryFull title
All authors
Cianciaruso C, Beltraminelli T, Duval F, Nassiri S, Hamelin R, Mozes A, Gallart-Ayala H, Ceada Torres G, Torchia B, Ries CH, Ivanisevic J, De Palma M
Journal
Cell Rep
Abstract
Extracellular vesicles (EVs), including exosomes, modulate multiple aspects of cancer biology. Tumor (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD81/ Alix/ CD9/ GAPDH
non-EV: Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
E0771
EV-harvesting Medium
Serum-containing, but physical separation of serum EVs and secreted EVs (e.g. Bioreactor flask)
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
35
Wash: time (min)
70
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
134,000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ GAPDH
Not detected EV-associated proteins
CD81
Characterization: Lipid analysis
No
|
||||||||
EV170031 | 1/1 | Homo sapiens | Serum |
(d)(U)C Filtration |
Ramanathan S | 2019 | 44% | |
Study summaryFull title
All authors
Ramanathan S, Douglas SR, Alexander GM, Shenoda BB, Barrett JE, Aradillas E, Sacan A, Ajit SK
Journal
J Transl Med
Abstract
BACKGROUND:
Therapeutic plasma exchange (PE) or plasmapheresis is an extracorporeal procedure employ (show more...)
EV-METRIC
44% (86th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Complex regional pain syndrome
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
142.9 (pelleting) / 142.9 (washing)
Protein markers
EV: CD81/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
110000
Pelleting: adjusted k-factor
142.9
Wash: time (min)
70
Wash: Rotor Type
Type 50.2 Ti
Wash: speed (g)
110000
Wash: adjusted k-factor
142.9
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63
Characterization: RNA analysis
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
85.7
EV concentration
Yes
Particle yield
394000000000
EM
EM-type
Transmission-EM/ Immune-EM
EM protein
CD81
Image type
Close-up, Wide-field
|
||||||||
EV230047 | 3/4 | Homo sapiens | Serum |
(d)(U)C Filtration UF |
Panigrahi GK | 2019 | 43% | |
Study summaryFull title
All authors
Panigrahi GK, Praharaj PP, Kittaka H, Mridha AR, Black OM, Singh R, Mercer R, van Bokhoven A, Torkko KC, Agarwal C, Agarwal R, Abd Elmageed ZY, Yadav H, Mishra SK, Deep G
Journal
Cancer Med
Abstract
African American men face a stark prostate cancer (PCa)-related health disparity, with the highest i (show more...)
EV-METRIC
43% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Ultrafiltration Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 70.1 Ti
Pelleting: speed (g)
100000
Filtration steps
0.2 or 0.22 µm
Ultra filtration
Cut-off size (kDa)
150
Membrane type
NS
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
175
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
180
EV concentration
Yes
|
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EV230047 | 4/4 | Homo sapiens | Serum |
(d)(U)C Filtration UF |
Panigrahi GK | 2019 | 43% | |
Study summaryFull title
All authors
Panigrahi GK, Praharaj PP, Kittaka H, Mridha AR, Black OM, Singh R, Mercer R, van Bokhoven A, Torkko KC, Agarwal C, Agarwal R, Abd Elmageed ZY, Yadav H, Mishra SK, Deep G
Journal
Cancer Med
Abstract
African American men face a stark prostate cancer (PCa)-related health disparity, with the highest i (show more...)
EV-METRIC
43% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Prostate cancer
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Ultrafiltration Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 70.1 Ti
Pelleting: speed (g)
100000
Filtration steps
0.2 or 0.22 µm
Ultra filtration
Cut-off size (kDa)
150
Membrane type
NS
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
175
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
170
EV concentration
Yes
|
||||||||
EV210430 | 1/2 | Homo sapiens | Bone-marrow derived mesenchymal stem cells | Total Exosome Isolation | Tracy SA | 2019 | 43% | |
Study summaryFull title
All authors
Tracy SA, Ahmed A, Tigges JC, Ericsson M, Pal AK, Zurakowski D, Fauza DO
Journal
J Pediatr Surg
Abstract
Exosomes may constitute a more practical alternative to live cells in select stem cell-based therapi (show more...)
EV-METRIC
43% (81st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Commercial method
Protein markers
EV: CD81/ CD63/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Bone-marrow derived mesenchymal stem cells
EV-harvesting Medium
Serum free medium
Separation Method
Commercial kit
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Flow cytometry specific beads
Antibody details provided?
Yes
Antibody dilution provided?
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Modus
Reported size (nm)
151
EV concentration
Yes
EM
EM-type
Immuno-EM/ Transmission-EM
EM protein
CD63
Image type
Close-up, Wide-field
|
||||||||
EV210430 | 2/2 | Homo sapiens | Amniotic fluid derived mesenchymal stem cells | Total Exosome Isolation | Tracy SA | 2019 | 43% | |
Study summaryFull title
All authors
Tracy SA, Ahmed A, Tigges JC, Ericsson M, Pal AK, Zurakowski D, Fauza DO
Journal
J Pediatr Surg
Abstract
Exosomes may constitute a more practical alternative to live cells in select stem cell-based therapi (show more...)
EV-METRIC
43% (81st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Commercial method
Protein markers
EV: CD81/ CD63/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Amniotic fluid derived mesenchymal stem cells
EV-harvesting Medium
Serum free medium
Separation Method
Commercial kit
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Flow cytometry specific beads
Antibody details provided?
Yes
Antibody dilution provided?
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Modus
Reported size (nm)
132
EV concentration
Yes
EM
EM-type
Immuno-EM/ Transmission-EM
EM protein
CD63
Image type
Close-up, Wide-field
|
||||||||
EV190020 | 3/3 | Rattus norvegicus | ASML |
DG (d)(U)C Filtration |
Kyuno, Daisuke | 2019 | 43% | |
Study summaryFull title
All authors
Kyuno D, Zhao K, Schnölzer M, Provaznik J, Hackert T, Zöller M.
Journal
Int J Cancer
Abstract
Claudin7 (cld7) is a cancer-initiating cell (CIC) marker in gastrointestinal tumors, a cld7-knockdow (show more...)
EV-METRIC
43% (81st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
claudin 7 knockdown
Focus vesicles
exosome
Separation protocol
Separation protocol
DG
(d)(U)C Filtration Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.15-1.56
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Rattus norvegicus
Sample Type
Cell culture supernatant
EV-producing cells
ASML
EV-harvesting Medium
Serum free medium
Cell viability (%)
100
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
50
Wash: time (min)
120
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
100000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
3
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
4
Sample volume (mL)
0.8
Orientation
Bottom-up
Rotor type
SW 41 Ti
Speed (g)
100000
Duration (min)
960
Fraction volume (mL)
1.28
Fraction processing
Centrifugation
Pelleting: volume per fraction
50
Pelleting: duration (min)
150
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Pelleting-wash: volume per pellet (mL)
50
Pelleting-wash: duration (min)
150
Pelleting-wash: speed (g)
Type 45 Ti
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
None
Protein Concentration Method
Bradford
Characterization: RNA analysis
RNA analysis
Type
Database
Proteinase treatment
RNAse treatment
Characterization: Lipid analysis
No
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