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You searched for: EV190016 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV190016 | 1/1 | Homo sapiens | Wharton's jelly mesenchymal stromal cells | (d)(U)C | Thomi, Gierin | 2019 | 44% | |
Study summaryFull title
All authors
Gierin Thomi, Daniel Surbek, Valérie Haesler, Marianne Joerger-Messerli, Andreina Schoeberlein
Journal
Stem Cell Res Ther
Abstract
Background: Preterm newborns are at high risk of developing neurodevelopmental deficits caused by ne (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ CD63/ CD81/ Alix/ ICAM/ Flotillin1/ EpCAM/ ANXA5
non-EV: GRP94/ GM130 Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Wharton's jelly mesenchymal stromal cells
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
TFT-70
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
5
Wash: time (min)
70
Wash: Rotor Type
TFT-70
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Antibody details provided?
No
Not detected contaminants
GRP94
Detected EV-associated proteins
Alix/ Flotillin1/ CD63/ EpCAM/ ICAM/ ANXA5/ CD81/ TSG101
Not detected contaminants
GM130
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
42.93
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1 - 1 of 1 |
EV-TRACK ID | EV190016 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | Wharton's jelly mesenchymal stromal cells |
condition | Control condition |
separation protocol | (d)(U)C |
Exp. nr. | 1 |
EV-METRIC % | 44 |