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Showing 1 - 50 of 830
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV170028 | 1/1 | Homo sapiens | Synovial fluid |
DG (d)(U)C SEC |
Foers AD | 2017 | 100% | |
Study summaryFull title
All authors
Foers AD, Chatfield S, Dagley LF, Scicluna BJ, Webb AI, Cheng L, Hill AF, Wicks IP, Pang KC.
Journal
J Extracell Vesicles
Abstract
As a complex biological fluid, human synovial fluid (SF) presents challenges for extracellular vesic (show more...)
EV-METRIC
100% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Synovial fluid
Sample origin
Arthritis
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C SEC Adj. k-factor
156.9 (pelleting) / 156.9 (washing)
Protein markers
EV: TSG101/ Rab27b/ Annexin1/ Syntenin/ HSP70/ Flotillin-1
non-EV: Fibronectin/ Albumin/ ApoA1 Proteomics
yes
Show all info
Study aim
New methodological development, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Synovial fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
156.9
Wash: time (min)
90
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
100000
Wash: adjusted k-factor
156.9
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
6
Lowest density fraction
0.25
Highest density fraction
2
Sample volume (mL)
0.65
Orientation
Top-down (sample migrates downwards)
Rotor type
SW 40 Ti
Speed (g)
202000
Duration (min)
1100
Fraction volume (mL)
1.05
Fraction processing
Centrifugation
Pelleting: volume per fraction
10
Pelleting: duration (min)
90
Pelleting: rotor type
Type 70.1Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
122.2
Size-exclusion chromatography
Total column volume (mL)
320
Sample volume/column (mL)
13
Resin type
Sephacryl S-500 HR
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
Flotillin-1, HSP70, TSG101, Syntenin, Rab27b, Annexin1
Not detected contaminants
Albumin, ApoA1
Proteomics database
Yes
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
x
EV concentration
Yes
Particle yield
x
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV170008 | 1/3 | Homo sapiens | primary monocyte derived dendritic cell |
DG (d)(U)C |
Mercedes Tkach | 2017 | 100% | |
Study summaryFull title
All authors
Tkach M, Kowal J, Zucchetti AE, Enserink L, Jouve M, Lankar D, Saitakis M, Martin-Jaular L, Théry C
Journal
EMBO J
Abstract
Exosomes, nano-sized secreted extracellular vesicles (EVs), are actively studied for their diagnosti (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: CD63/ MHC2/ CD9/ CD9,MHC2
non-EV: None Proteomics
no
Show all info
Study aim
EV functional activity
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary monocyte derived dendritic cell
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Cell viability (%)
NA
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
Yes
Pelleting: time(min)
20
Pelleting: rotor type
Swinging bucket
Pelleting: speed (g)
2000
Wash: time (min)
20
Wash: Rotor Type
Eppendorf 5810R cf; swinging bucket
Wash: speed (g)
2000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
3
Lowest density fraction
0.1
Highest density fraction
0.3
Sample volume (mL)
1.2
Orientation
Bottom-up (sample migrates upwards)
Rotor type
SW 55 Ti
Speed (g)
350000
Duration (min)
60
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
2.5
Pelleting: duration (min)
30
Pelleting: rotor type
TLA-110
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
65.69
EV-subtype
Distinction between multiple subtypes
Centrifugation steps: 2K, 10K, 100K
PMID previous EV protein analysis
26858453
Protein Concentration Method
microBCA
Protein Yield (µg)
2.9+-0.3
Western Blot
Detected EV-associated proteins
CD9,MHC2
Flow cytometry
Type of Flow cytometry
MACSQuant Miltenyi
Calibration bead size
0.1-0.3,0.4-0.6,0.7-0.9,1.0-1.9
Characterization: Lipid analysis
No
Characterization: Particle analysis
PMID previous EV particle analysis
26858453
Extra particle analysis
NTA
Report type
Size range/distribution
EV concentration
Yes
Particle yield
6.52E10+-2.03E10 particles/million cells
EM
EM-type
Transmission-EM/ Scanning-EM
EM protein
MHC2
Image type
Close-up, Wide-field
|
||||||||
EV170008 | 2/3 | Homo sapiens | primary monocyte derived dendritic cell |
DG (d)(U)C |
Mercedes Tkach | 2017 | 100% | |
Study summaryFull title
All authors
Tkach M, Kowal J, Zucchetti AE, Enserink L, Jouve M, Lankar D, Saitakis M, Martin-Jaular L, Théry C
Journal
EMBO J
Abstract
Exosomes, nano-sized secreted extracellular vesicles (EVs), are actively studied for their diagnosti (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Adj. k-factor
2097 (pelleting) / 2097 (washing)
Protein markers
EV: CD63/ MHC2/ CD9/ CD9,MHC2
non-EV: None Proteomics
no
Show all info
Study aim
EV functional activity
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary monocyte derived dendritic cell
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Cell viability (%)
NA
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
40
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
10000
Pelleting: adjusted k-factor
2097.
Wash: time (min)
40
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
10000
Wash: adjusted k-factor
2097.
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
3
Lowest density fraction
0.1
Highest density fraction
0.3
Sample volume (mL)
1.2
Orientation
Bottom-up (sample migrates upwards)
Rotor type
SW 55 Ti
Speed (g)
350000
Duration (min)
60
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
2.5
Pelleting: duration (min)
30
Pelleting: rotor type
TLA-110
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
65.69
EV-subtype
Distinction between multiple subtypes
Centrifugation steps: 2K, 10K, 100K
PMID previous EV protein analysis
26858453
Protein Concentration Method
microBCA
Protein Yield (µg)
1.7+-0.3
Western Blot
Detected EV-associated proteins
CD9,MHC2
Flow cytometry
Type of Flow cytometry
MACSQuant Miltenyi
Calibration bead size
0.1-0.3,0.4-0.6,0.7-0.9,1.0-1.9
Characterization: Lipid analysis
No
Characterization: Particle analysis
PMID previous EV particle analysis
26858453
Extra particle analysis
NTA
Report type
Size range/distribution
EV concentration
Yes
Particle yield
4.95E10+-3.81E10 particles/million cells
EM
EM-type
Transmission-EM/ Scanning-EM
EM protein
MHC2
Image type
Close-up, Wide-field
|
||||||||
EV170008 | 3/3 | Homo sapiens | primary monocyte derived dendritic cell |
DG (d)(U)C |
Mercedes Tkach | 2017 | 100% | |
Study summaryFull title
All authors
Tkach M, Kowal J, Zucchetti AE, Enserink L, Jouve M, Lankar D, Saitakis M, Martin-Jaular L, Théry C
Journal
EMBO J
Abstract
Exosomes, nano-sized secreted extracellular vesicles (EVs), are actively studied for their diagnosti (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Adj. k-factor
209.7 (pelleting) / 209.7 (washing)
Protein markers
EV: CD63/ MHC2/ CD9/ CD9,MHC2
non-EV: None Proteomics
no
Show all info
Study aim
EV functional activity
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary monocyte derived dendritic cell
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Cell viability (%)
NA
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
209.7
Wash: time (min)
90
Wash: Rotor Type
Type 45 Ti
Wash: speed (g)
100000
Wash: adjusted k-factor
209.7
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
3
Lowest density fraction
0.1
Highest density fraction
0.3
Sample volume (mL)
1.2
Orientation
Bottom-up (sample migrates upwards)
Rotor type
SW 55 Ti
Speed (g)
350000
Duration (min)
60
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
2.5
Pelleting: duration (min)
30
Pelleting: rotor type
TLA-110
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
65.69
EV-subtype
Distinction between multiple subtypes
Centrifugation steps: 2K, 10K, 100K
PMID previous EV protein analysis
26858453
Protein Concentration Method
microBCA
Protein Yield (µg)
1.1+-0.2
Western Blot
Detected EV-associated proteins
CD9,MHC2
Flow cytometry
Type of Flow cytometry
MACSQuant Miltenyi
Calibration bead size
0.1-0.3,0.4-0.6,0.7-0.9,1.0-1.9
Characterization: Lipid analysis
No
Characterization: Particle analysis
PMID previous EV particle analysis
26858453
Extra particle analysis
NTA
Report type
Size range/distribution
EV concentration
Yes
Particle yield
3.76E10+-1.41E10 particles/million cells
EM
EM-type
Transmission-EM/ Scanning-EM
EM protein
MHC2
Image type
Close-up, Wide-field
|
||||||||
EV170001 | 1/1 | Homo sapiens | MCF7 Rab27b-GFP |
DG (d)(U)C Filtration UF |
Vergauwen, Glenn | 2017 | 100% | |
Study summaryFull title
All authors
Vergauwen G, Dhondt B, Van Deun J, De Smedt E, Berx G, Timmerman E, Gevaert K, Miinalainen I, Cocquyt V, Braems G, Van den Broecke R, Denys H, De Wever O, Hendrix A.
Journal
Sci Rep
Abstract
Identification and validation of extracellular vesicle (EV)-associated biomarkers requires robust is (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Filtration UF Protein markers
EV: Alix/ CD81/ TSG101
non-EV: PMP70/ Argonaute-2 Proteomics
no
EV density (g/ml)
1.094
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MCF7 Rab27b-GFP
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
>=18h at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
16.5
Sample volume (mL)
1
Orientation
Top-down
Rotor type
SW 32.1 Ti
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
16
Pelleting: duration (min)
180
Pelleting: rotor type
SW 32.1 Ti
Pelleting: speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm,
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Regenerated cellulose
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,…)
Western Blot
Detected EV-associated proteins
Alix/ CD81/ TSG101
Not detected contaminants
Argonaute-2/ PMP70
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
113
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV170046 | 1/2 | Sus scrofa | Primary retinal pigmented epithelium cells |
DG (d)(U)C UF |
Klingeborn M | 2017 | 88% | |
Study summaryFull title
All authors
Klingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C
Journal
Sci Rep
Abstract
The retinal pigmented epithelium (RPE) forms the outer blood-retinal barrier in the eye and its pola (show more...)
EV-METRIC
88% (98th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Basolateral side of polarized layer
Focus vesicles
exosome
Separation protocol
Separation protocol
DG
(d)(U)C UF Adj. k-factor
253.9 (pelleting) / 253.9 (washing)
Protein markers
EV: TSG101/ Syntenin-1
non-EV: Calreticulin Proteomics
yes
Show all info
Study aim
New methodological development, Identification of content (omics approaches)
Sample
Species
Sus scrofa
Sample Type
Cell culture supernatant
EV-producing cells
Primary retinal pigmented epithelium cells
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
253.9
Wash: time (min)
90
Wash: Rotor Type
SW 28
Wash: speed (g)
100000
Wash: adjusted k-factor
253.9
Density gradient
Only used for validation of main results
Yes
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
0.05
Highest density fraction
0.4
Sample volume (mL)
1
Orientation
Bottom-up (sample migrates upwards)
Rotor type
SW 41 Ti
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
12
Pelleting: duration (min)
90
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
255.8
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Characterization: Protein analysis
Protein Concentration Method
Pierce 660nm Protein Assay
Western Blot
Detected EV-associated proteins
TSG101, Syntenin-1
Not detected contaminants
Calreticulin
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
132.2±13.1
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV170046 | 2/2 | Sus scrofa | Primary retinal pigmented epithelium cells |
DG (d)(U)C UF |
Klingeborn M | 2017 | 88% | |
Study summaryFull title
All authors
Klingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C
Journal
Sci Rep
Abstract
The retinal pigmented epithelium (RPE) forms the outer blood-retinal barrier in the eye and its pola (show more...)
EV-METRIC
88% (98th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Apical side of polarized layer
Focus vesicles
exosome
Separation protocol
Separation protocol
DG
(d)(U)C UF Adj. k-factor
253.9 (pelleting) / 253.9 (washing)
Protein markers
EV: TSG101/ Syntenin-1
non-EV: Calreticulin Proteomics
yes
Show all info
Study aim
New methodological development, Identification of content (omics approaches)
Sample
Species
Sus scrofa
Sample Type
Cell culture supernatant
EV-producing cells
Primary retinal pigmented epithelium cells
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
253.9
Wash: time (min)
90
Wash: Rotor Type
SW 28
Wash: speed (g)
100000
Wash: adjusted k-factor
253.9
Density gradient
Only used for validation of main results
Yes
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
0.05
Highest density fraction
0.4
Sample volume (mL)
1
Orientation
Bottom-up (sample migrates upwards)
Rotor type
SW 41 Ti
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
12
Pelleting: duration (min)
90
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
255.8
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Characterization: Protein analysis
Protein Concentration Method
Pierce 660nm Protein Assay
Western Blot
Detected EV-associated proteins
TSG101, Syntenin-1
Not detected contaminants
Calreticulin
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
118.6±9.9
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV170016 | 1/2 | Mus musculus | adipose tissue-derived macrophages |
DG (d)(U)C Filtration |
Ying, Wei | 2017 | 88% | |
Study summaryFull title
All authors
Ying W, Riopel M, Bandyopadhyay G, Dong Y, Birmingham A, Seo JB, Ofrecio JM, Wollam J, Hernandez-Carretero A, Fu W, Li P, Olefsky JM
Journal
Cell
Abstract
MiRNAs are regulatory molecules that can be packaged into exosomes and secreted from cells. Here, we (show more...)
EV-METRIC
88% (98th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Obesitas
Focus vesicles
exosome
Separation protocol
Separation protocol
DG
(d)(U)C Filtration Adj. k-factor
256 (pelleting) / 256 (washing)
Protein markers
EV: TSG101/ HSP70/ Syntenin1/ CD63/ CD9
non-EV: Grp94 Proteomics
no
Show all info
Study aim
Function, Mechanism of uptake/transfer
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
adipose tissue-derived macrophages
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
240-360
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
256.0
Wash: time (min)
20
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
100000
Wash: adjusted k-factor
256.0
Density gradient
Only used for validation of main results
Yes
Density medium
156.9
Type
Discontinuous
Lowest density fraction
0.1
Highest density fraction
0.3
Sample volume (mL)
0.3
Orientation
Bottom-up (sample migrates upwards)
Rotor type
Type 70 Ti
Speed (g)
350000
Duration (min)
60
Fraction volume (mL)
2.4
Fraction processing
Centrifugation
Pelleting: volume per fraction
2
Pelleting: duration (min)
90
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
156.9
Pelleting-wash: volume per pellet (mL)
24
Pelleting-wash: duration (min)
30
Pelleting-wash: rotor type
156.9
Pelleting-wash: speed (g)
Type 70 Ti
Pelleting-wash: adjusted k-factor
156.9
Filtration steps
0.22µm or 0.2µm
EV-subtype
Distinction between multiple subtypes
1.13-1.15 g/ml
Used subtypes
Yes
Characterization: Protein analysis
Protein Concentration Method
DC protein assay
Protein Yield (µg)
9-May
Western Blot
Detected EV-associated proteins
CD9, CD63, HSP70, TSG101, Syntenin1
Not detected contaminants
Grp94
Characterization: RNA analysis
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
30-150
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
50-200 nm
Extra information
Full UC and density gradient protocols not in original article
|
||||||||
EV170004 | 1/1 | Homo sapiens | Adipose tissue |
DG (d)(U)C Filtration |
Jeurissen S | 2017 | 88% | |
Study summaryFull title
All authors
Jeurissen S, Vergauwen G, Van Deun J, Lapeire L, Depoorter V, Miinalainen I, Sormunen R, Van den Broecke R, Braems G, Cocquyt V, Denys H, Hendrix A
Journal
Cell Adh Migr
Abstract
Breast cancer cells closely interact with different cell types of the surrounding adipose tissue to (show more...)
EV-METRIC
88% (91st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Adipose tissue
Sample origin
breast cancer
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Filtration Adj. k-factor
138.6 (pelleting) / 138.6 (washing)
Protein markers
EV: CD63/ HSP70/ FABP4/ Flotillin-1/ CD9
non-EV: Prohibitin/ GM130/ Calreticulin Proteomics
no
Show all info
Study aim
Function, Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Adipose tissue
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW 55 Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
138.6
Wash: time (min)
120
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Wash: adjusted k-factor
138.6
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
0.05
Highest density fraction
0.4
Sample volume (mL)
1
Orientation
Top-down (sample migrates downwards)
Rotor type
SW 32.1 Ti
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
16
Pelleting: duration (min)
180
Pelleting: rotor type
SW 32.1 Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
297.9
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Western Blot
Detected EV-associated proteins
CD9, Flotillin-1, HSP70, FABP4
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
116
EV concentration
Yes
Particle yield
2.2 10E09
EM
EM-type
Immune-EM
EM protein
CD63
Image type
Close-up, Wide-field
Report size (nm)
20-200
|
||||||||
EV210202 | 1/7 | Mus musculus | 3T3-L1 |
DG (d)(U)C |
Durcin, Maëva | 2017 | 78% | |
Study summaryFull title
All authors
Maëva Durcin, Audrey Fleury, Emiliane Taillebois, Grégory Hilairet, Zuzana Krupova, Céline Henry, Sandrine Truchet, Martin Trötzmüller, Harald Köfeler, Guillaume Mabilleau, Olivier Hue, Ramaroson Andriantsitohaina, Patrice Martin, Soazig Le Lay
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) are biological vectors that can modulate the metabolism of target cells (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Large extracellular vesicles
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: None
non-EV: None Proteomics
yes
EV density (g/ml)
1.14-1.24
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
3T3-L1
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
MLA-50
Pelleting: speed (g)
13000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
60
Wash: Rotor Type
MLA-50
Wash: speed (g)
13000
Density gradient
Only used for validation of main results
Yes
Type
Discontinuous
Number of initial discontinuous layers
31
Lowest density fraction
0.4M
Highest density fraction
2M
Total gradient volume, incl. sample (mL)
12000
Sample volume (mL)
500
Orientation
Top-down
Rotor type
Not specified
Speed (g)
200000
Duration (min)
1080
Fraction volume (mL)
1000
Fraction processing
Centrifugation
Pelleting: volume per fraction
6000
Pelleting: duration (min)
70
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Detected EV-associated proteins
CD9/ CD63/ Mfge8/ caveolin-1/ b-actin/ Flotillin2/ TSG101/ Alix
Not detected EV-associated proteins
CD81
Flow cytometry
Type of Flow cytometry
MACSQuant flow cytometer
Calibration bead size
4µm
Detected EV-associated proteins
Annexin V
Proteomics database
No
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
160
EV concentration
Yes
Particle yield
EV secreted per adipocyte;Yes, other: 11
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
30-100
|
||||||||
EV210202 | 2/7 | Mus musculus | 3T3-L1 |
DG (d)(U)C |
Durcin, Maëva | 2017 | 78% | |
Study summaryFull title
All authors
Maëva Durcin, Audrey Fleury, Emiliane Taillebois, Grégory Hilairet, Zuzana Krupova, Céline Henry, Sandrine Truchet, Martin Trötzmüller, Harald Köfeler, Guillaume Mabilleau, Olivier Hue, Ramaroson Andriantsitohaina, Patrice Martin, Soazig Le Lay
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) are biological vectors that can modulate the metabolism of target cells (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Small extracellular vesicles
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: None
non-EV: None Proteomics
yes
EV density (g/ml)
1.14-1.20
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
3T3-L1
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
MLA-50
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
60
Wash: Rotor Type
MLA-50
Wash: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Type
Discontinuous
Number of initial discontinuous layers
31
Lowest density fraction
0.4M
Highest density fraction
2M
Total gradient volume, incl. sample (mL)
12000
Sample volume (mL)
500
Orientation
Top-down
Rotor type
Not specified
Speed (g)
200000
Duration (min)
1080
Fraction volume (mL)
1000
Fraction processing
Centrifugation
Pelleting: volume per fraction
6000
Pelleting: duration (min)
70
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Detected EV-associated proteins
CD9/ CD63/ Mfge8/ Flotillin2/ TSG101/ Alix/ CD81
Not detected EV-associated proteins
caveolin-1/ b-actin
Flow cytometry
Type of Flow cytometry
MACSQuant flow cytometer
Calibration bead size
4µm
Not detected EV-associated proteins
Annexin V
Proteomics database
No
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
100
EV concentration
Yes
Particle yield
EV secreted per adipocyte;Yes, other: 843
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
90-800
|
||||||||
EV170067 | 1/6 | Homo sapiens | HMC-1 |
DG (d)(U)C |
Lässer C | 2017 | 78% | |
Study summaryFull title
All authors
Lässer C, Shelke GV, Yeri A, Kim DK, Crescitelli R, Raimondo S, Sjöstrand M, Gho YS, Van Keuren Jensen K, Lötvall J.
Journal
RNA Biol
Abstract
Cells secrete extracellular RNA (exRNA) to their surrounding environment and exRNA has been found in (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Extracellular vesicle-like structures
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: TSG101/ CD63/ CD81/ Flotillin1/ ANXA2/ CD9
non-EV: Proteomics
yes
EV density (g/ml)
1.09 - 1.31
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HMC-1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
120000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
16
Lowest density fraction
0.4M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
35
Sample volume (mL)
2
Orientation
Bottom-up
Rotor type
SW 32 Ti
Speed (g)
175000
Duration (min)
960
Fraction volume (mL)
3.9
Fraction processing
Centrifugation
Pelleting: duration (min)
70
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
120000
EV-subtype
Distinction between multiple subtypes
Density
Used subtypes
1.09-1.21 g/mL
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
Flotillin1/ ANXA2/ TSG101/ CD81
Flow cytometry specific beads
Detected EV-associated proteins
CD9/ CD63/ CD81
Proteomics database
Yes
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing;Microarray
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Immuno-EM
EM protein
CD63
Image type
Close-up, Wide-field
Report size (nm)
92
|
||||||||
EV170064 | 1/2 | Bos taurus | Milk |
DG (d)(U)C |
Samuel, M | 2017 | 78% | |
Study summaryFull title
All authors
Samuel M, Chisanga D, Liem M, Keerthikumar S, Anand S, Ang CS, Adda CG, Versteegen E, Jois M, Mathivanan S
Journal
Abstract
Exosomes are extracellular vesicles secreted by multiple cell types into the extracellular space. Th (show more...)
EV-METRIC
78% (86th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Sample origin
Control condition
Focus vesicles
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: Alix/ TSG101
non-EV: Albumin/ GM130 Proteomics
yes
EV density (g/ml)
1.16
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Bos taurus
Sample Type
Milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
1080
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
0.5
Wash: time (min)
60
Wash: Rotor Type
SW 28
Wash: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
0.5
Orientation
Top-down
Rotor type
SW 40 Ti
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
None
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
TSG101
Not detected EV-associated proteins
Alix
Detected contaminants
Albumin
Not detected contaminants
GM130
Proteomics database
Yes
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
145
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV170064 | 2/2 | Bos taurus | Milk |
DG (d)(U)C |
Samuel, M | 2017 | 78% | |
Study summaryFull title
All authors
Samuel M, Chisanga D, Liem M, Keerthikumar S, Anand S, Ang CS, Adda CG, Versteegen E, Jois M, Mathivanan S
Journal
Abstract
Exosomes are extracellular vesicles secreted by multiple cell types into the extracellular space. Th (show more...)
EV-METRIC
78% (86th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Sample origin
Colostrum
Focus vesicles
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: / Alix/ TSG101
non-EV: Albumin/ GM130 Proteomics
yes
EV density (g/ml)
1.16
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Bos taurus
Sample Type
Milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
1080
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
0.5
Wash: time (min)
60
Wash: Rotor Type
SW 28
Wash: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
0.5
Orientation
Top-down
Rotor type
SW 40 Ti
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
None
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
Alix/ TSG101
Not detected EV-associated proteins
Detected contaminants
Albumin
Not detected contaminants
GM130
Proteomics database
Yes
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
125
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV170065 | 1/1 | Homo sapiens | Primary tumor-derived fibroblasts | (d)(U)C | Bhome R | 2017 | 77% | |
Study summaryFull title
All authors
Bhome R, Goh RW1, Bullock MD, Pillar N, Thirdborough SM, Mellone M, Mirnezami R, Galea D, Veselkov K, Gu Q, Underwood TJ, Primrose JN, De Wever O, Shomron N, Sayan AE, Mirnezami AH.
Journal
Aging (Albany NY)
Abstract
Colorectal cancer is a global disease with increasing incidence. Mortality is largely attributed to (show more...)
EV-METRIC
77% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Colorectal cancer
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
88.25 (pelleting) / 88.25 (washing)
Protein markers
EV: Alix/ CD81/ TSG101/ CD63
non-EV: cytochrome c/ GM130/ cytochromec Proteomics
no
Show all info
Study aim
Function, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Primary tumor-derived fibroblasts
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 50.3 Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
88.25
Wash: time (min)
70
Wash: Rotor Type
Type 50.3 Ti
Wash: speed (g)
100000
Wash: adjusted k-factor
88.25
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
100
Western Blot
Detected EV-associated proteins
Alix, CD63, CD81, TSG101
Not detected contaminants
GM130, cytochrome c
Characterization: RNA analysis
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
PMID previous EV particle analysis
Electron microscopy
Extra particle analysis
NTA
Report type
Modus
Reported size (nm)
113
EV concentration
Yes
Particle yield
1570000000000
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV170063 | 1/2 | Homo sapiens | Jurkat |
DG (d)(U)C Filtration |
Nemeth, Andrea | 2017 | 75% | |
Study summaryFull title
All authors
Németh A, Orgovan N, Sódar BW, Osteikoetxea X, Pálóczi K, Szabó-Taylor KÉ, Vukman KV, Kittel Á, Turiák L, Wiener Z, Tóth S, Drahos L, Vékey K, Horvath R, Buzás EI.
Journal
Sci Rep
Abstract
Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesi (show more...)
EV-METRIC
75% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Cyprofloxacin
Focus vesicles
exosome
Separation protocol
Separation protocol
DG
(d)(U)C Filtration Adj. k-factor
115.3 (pelleting) / 115.3 (washing)
Protein markers
EV: CD63/ HistoneH2B
non-EV: None Proteomics
yes
Show all info
Study aim
Biogenesis/cargo sorting, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Jurkat
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
MLA-55
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
115.3
Wash: time (min)
70
Wash: Rotor Type
MLA-55
Wash: speed (g)
100000
Wash: adjusted k-factor
115.3
Density gradient
Only used for validation of main results
Yes
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
0.05
Highest density fraction
0.4
Sample volume (mL)
0.5
Orientation
Top-down (sample migrates downwards)
Rotor type
MLS-50
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
0.5
Fraction processing
Centrifugation
Pelleting: duration (min)
180
Pelleting: speed (g)
100000
Filtration steps
> 0.45 µm, 0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
No
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
TRPS
Report type
Size range/distribution
Reported size (nm)
50-200
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV170063 | 2/2 | Homo sapiens | Jurkat |
DG (d)(U)C Filtration |
Nemeth, Andrea | 2017 | 75% | |
Study summaryFull title
All authors
Németh A, Orgovan N, Sódar BW, Osteikoetxea X, Pálóczi K, Szabó-Taylor KÉ, Vukman KV, Kittel Á, Turiák L, Wiener Z, Tóth S, Drahos L, Vékey K, Horvath R, Buzás EI.
Journal
Sci Rep
Abstract
Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesi (show more...)
EV-METRIC
75% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
DG
(d)(U)C Filtration Adj. k-factor
115.3 (pelleting) / 115.3 (washing)
Protein markers
EV: CD63
non-EV: None Proteomics
yes
Show all info
Study aim
Biogenesis/cargo sorting, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Jurkat
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
MLA-55
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
115.3
Wash: time (min)
70
Wash: Rotor Type
MLA-55
Wash: speed (g)
100000
Wash: adjusted k-factor
115.3
Density gradient
Only used for validation of main results
Yes
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
0.05
Highest density fraction
0.4
Sample volume (mL)
0.5
Orientation
Top-down (sample migrates downwards)
Rotor type
MLS-50
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
0.5
Fraction processing
Centrifugation
Pelleting: duration (min)
180
Pelleting: speed (g)
100000
Filtration steps
> 0.45 µm, 0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
No
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
TRPS
Report type
Size range/distribution
Reported size (nm)
50-200
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV170017 | 1/1 | Mus musculus | Bronchoalveolar lavage fluid | (d)(U)C | Maroto, Rosario | 2017 | 75% | |
Study summaryFull title
All authors
Maroto R, Zhao Y, Jamaluddin M, Popov VL, Wang H, Kalubowilage M, Zhang Y, Luisi J, Sun H, Culbertson CT, Bossmann SH, Motamedi M, Brasier AR
Journal
J Extracell Vesicles
Abstract
Background: Extracellular vesicles contain biological molecules specified by cell-type of origin and (show more...)
EV-METRIC
75% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bronchoalveolar lavage fluid
Sample origin
poly IC stimulated
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
60.38 (pelleting) / 60.38 (washing)
Protein markers
EV: Alix/ CD63,HSP90,Alix/ HSP90/ CD63
non-EV: Grp94,beta-actin/ Grp94/ beta-actin Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Bronchoalveolar lavage fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
TLA-100.3
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
60.38
Wash: time (min)
60
Wash: Rotor Type
TLA-100.3
Wash: speed (g)
100000
Wash: adjusted k-factor
60.38
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD63,HSP90,Alix
Not detected contaminants
Grp94,beta-actin
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Median
Reported size (nm)
95
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230813 | 2/6 | Escherichia coli | W3110 |
(d)(U)C DG Filtration UF |
Kim OY | 2017 | 72% | |
Study summaryFull title
All authors
Kim OY, Park HT, Dinh NTH, Choi SJ, Lee J, Kim JH, Lee SW, Gho YS
Journal
Nat Commun
Abstract
Gram-negative bacteria actively secrete outer membrane vesicles, spherical nano-meter-sized proteoli (show more...)
EV-METRIC
72% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
msbB deletion mutant
Focus vesicles
outer membrane vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Ultrafiltration Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Escherichia coli
Sample Type
Cell culture supernatant
EV-producing cells
W3110
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
180
Pelleting: speed (g)
150000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
3
Lowest density fraction
10%
Highest density fraction
50%
Orientation
Bottom-up
Speed (g)
200000
Duration (min)
120
Fraction processing
None
Filtration steps
0.2 or 0.22 µm/ 0.45 µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Hollowfiber
Characterization: Protein analysis
Protein Concentration Method
Bradford
Proteomics database
ProteomeXchange Consortium
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
38.7
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV230753 | 1/1 | Aggregatibacter actinomycetemcomitans | 173 |
(d)(U)C DG Filtration |
Kieselbach T | 2017 | 72% | |
Study summaryFull title
All authors
Kieselbach T, Oscarsson J
Journal
Data Brief
Abstract
The Gram-negative bacterium is an oral and systemic pathogen, which is linked to aggressive forms o (show more...)
EV-METRIC
72% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
outer membrane vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Adj. k-factor
15195 (pelleting) / 0 (washing)
Protein markers
EV: None
non-EV: None Proteomics
yes
EV density (g/ml)
not specified
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Aggregatibacter actinomycetemcomitans
Sample Type
Cell culture supernatant
EV-producing cells
173
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
85 000
Pelleting: adjusted k-factor
15195
Wash: volume per pellet (ml)
not specified
Wash: time (min)
120
Wash: speed (g)
85000
Wash: adjusted k-factor
TDB
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
7
Lowest density fraction
10%
Highest density fraction
45%
Total gradient volume, incl. sample (mL)
4,17
Sample volume (mL)
0,15
Orientation
Bottom-up
Speed (g)
180000
Duration (min)
180
Fraction volume (mL)
0,2
Fraction processing
Centrifugation
Pelleting: volume per fraction
not spec
Pelleting: speed (g)
16000
Pelleting: adjusted k-factor
95526
Pelleting-wash: volume per pellet (mL)
not spec
Pelleting-wash: speed (g)
JA-18.1
Filtration steps
0.2 or 0.22 µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
ProteomeXchange
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Atomic force microscopy
|
||||||||
EV230812 | 2/14 | Streptomyces sp. Mg1 | PSK0558 |
(d)(U)C DG Filtration |
Hoefler BC | 2017 | 71% | |
Study summaryFull title
All authors
Hoefler BC, Stubbendieck RM, Josyula NK, Moisan SM, Schulze EM, Straight PD
Journal
Cell Chem Biol
Abstract
Specialized metabolites support bacterial competitive fitness as antibiotics, signals, pigments, and (show more...)
EV-METRIC
71% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
ΔlnyI
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Protein markers
EV: None
non-EV: None Proteomics
yes
EV density (g/ml)
not specified
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Streptomyces sp. Mg1
Sample Type
Cell culture supernatant
EV-producing cells
PSK0558
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
235000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
8
Lowest density fraction
10%
Highest density fraction
50%
Total gradient volume, incl. sample (mL)
14
Sample volume (mL)
1
Orientation
Bottom-up
Speed (g)
100400
Duration (min)
960
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
4mL
Pelleting: speed (g)
120000
Pelleting: adjusted k-factor
1.857
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
EM
EM-type
Transmission-EM
Image type
Wide-field
EV concentration
Yes
|
||||||||
EV230812 | 3/14 | Streptomyces sp. Mg1 | PSK0558 |
(d)(U)C DG Filtration |
Hoefler BC | 2017 | 71% | |
Study summaryFull title
All authors
Hoefler BC, Stubbendieck RM, Josyula NK, Moisan SM, Schulze EM, Straight PD
Journal
Cell Chem Biol
Abstract
Specialized metabolites support bacterial competitive fitness as antibiotics, signals, pigments, and (show more...)
EV-METRIC
71% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
ΔlnyI + lnyI (complement)
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Protein markers
EV: None
non-EV: None Proteomics
yes
EV density (g/ml)
not specified
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Streptomyces sp. Mg1
Sample Type
Cell culture supernatant
EV-producing cells
PSK0558
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
235000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
8
Lowest density fraction
10%
Highest density fraction
50%
Total gradient volume, incl. sample (mL)
14
Sample volume (mL)
1
Orientation
Bottom-up
Speed (g)
100400
Duration (min)
960
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
4mL
Pelleting: speed (g)
120000
Pelleting: adjusted k-factor
1.857
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
EM
EM-type
Transmission-EM
Image type
Wide-field
EV concentration
Yes
|
||||||||
EV230783 | 1/1 | Salmonella enterica | chi 3744 |
(d)(U)C DG Filtration |
Liu Q | 2017 | 67% | |
Study summaryFull title
All authors
Liu Q, Yi J, Liang K, Zhang X, Liu Q
Journal
J Microbiol Biotechnol
Abstract
Foodborne contamination and salmonellosis caused by Enteritidis (. Enteritidis) are a significant t (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
outer membrane vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Protein markers
EV: None
non-EV: SopD/ TufA/ SEN0876 Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Salmonella enterica
Sample Type
Cell culture supernatant
EV-producing cells
chi 3744
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: speed (g)
40000
Density gradient
Type
Discontinuous
Lowest density fraction
20%
Highest density fraction
45%
Speed (g)
150000
Duration (min)
overnight
Fraction processing
Centrifugation
Pelleting: speed (g)
40000
Filtration steps
Between 0.22 and 0.45 µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Proteomics database
No
Detected contaminants
SopD/ TufA/ SEN0876
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
None
|
||||||||
EV170067 | 2/6 | Homo sapiens | HMC-1 |
DG (d)(U)C Filtration |
Lässer C | 2017 | 67% | |
Study summaryFull title
All authors
Lässer C, Shelke GV, Yeri A, Kim DK, Crescitelli R, Raimondo S, Sjöstrand M, Gho YS, Van Keuren Jensen K, Lötvall J.
Journal
RNA Biol
Abstract
Cells secrete extracellular RNA (exRNA) to their surrounding environment and exRNA has been found in (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Extracellular vesicle-like structures
Separation protocol
Separation protocol
DG
(d)(U)C Filtration Protein markers
EV: TSG101/ CD63/ CD81/ Flotillin1/ ANXA2/ CD9
non-EV: Proteomics
yes
EV density (g/ml)
1.09-1.31
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HMC-1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
120000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
16
Lowest density fraction
0.4M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
35
Sample volume (mL)
2
Orientation
Bottom-up
Rotor type
SW 32 Ti
Speed (g)
175000
Duration (min)
960
Fraction volume (mL)
3.9
Fraction processing
Centrifugation
Pelleting: duration (min)
70
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
120000
Filtration steps
0.22µm or 0.2µm
EV-subtype
Distinction between multiple subtypes
Density
Used subtypes
1.24-1.31 g/mL
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
Flotillin1/ TSG101/ CD81
Not detected EV-associated proteins
ANXA2
Flow cytometry specific beads
Detected EV-associated proteins
CD9/ CD63/ CD81
Proteomics database
Yes
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing;Microarray
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Immuno-EM
EM protein
CD63
Image type
Wide-field
Report size (nm)
51
|
||||||||
EV220172 | 1/2 | Homo sapiens | Blood plasma |
SEC (non-commercial) UF |
Endzeliņš E | 2017 | 63% | |
Study summaryFull title
All authors
Endzeliņš E, Berger A, Melne V, Bajo-Santos C, Soboļevska K, Ābols A, Rodriguez M, Šantare D, Rudņickiha A, Lietuvietis V, Llorente A, Linē A
Journal
BMC Cancer
Abstract
Circulating cell-free miRNAs have emerged as promising minimally-invasive biomarkers for early detec (show more...)
EV-METRIC
63% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Benign prostatic hyperplasia
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Size-exclusion chromatography (non-commercial)
Ultrafiltration Protein markers
EV: CD9/ TSG101/ Actin beta
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Ultra filtration
Cut-off size (kDa)
3 Kda
Membrane type
Regenerated cellulose
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
0.4
Resin type
IsoaddSECResinType
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD9/ TSG101/ Actin beta
Not detected contaminants
Calnexin
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Proteinase treatment
Yes
Moment of Proteinase treatment
After
Proteinase type
Proteinase K
Proteinase concentration
1000
RNAse treatment
Yes
RNAse type
RNase A
RNAse concentration
10
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
50-150
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
25-60
|
||||||||
EV220172 | 2/2 | Homo sapiens | Blood plasma |
SEC (non-commercial) UF |
Endzeliņš E | 2017 | 63% | |
Study summaryFull title
All authors
Endzeliņš E, Berger A, Melne V, Bajo-Santos C, Soboļevska K, Ābols A, Rodriguez M, Šantare D, Rudņickiha A, Lietuvietis V, Llorente A, Linē A
Journal
BMC Cancer
Abstract
Circulating cell-free miRNAs have emerged as promising minimally-invasive biomarkers for early detec (show more...)
EV-METRIC
63% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Prostate cancer
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Size-exclusion chromatography (non-commercial)
Ultrafiltration Protein markers
EV: CD9/ TSG101/ Actin beta
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Ultra filtration
Cut-off size (kDa)
3 Kda
Membrane type
Regenerated cellulose
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
0.4
Resin type
IsoaddSECResinType
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD9/ TSG101/ Actin beta
Not detected contaminants
Calnexin
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Proteinase treatment
Yes
Moment of Proteinase treatment
After
Proteinase type
Proteinase K
Proteinase concentration
1000
RNAse treatment
Yes
RNAse type
RNase A
RNAse concentration
10
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
50-150
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
25-60
|
||||||||
EV170061 | 1/3 | Homo sapiens | BEAS2B |
(d)(U)C Filtration SEC UF |
Benedikter BJ | 2017 | 62% | |
Study summaryFull title
All authors
Benedikter BJ, Bouwman FG, Vajen T, Heinzmann ACA, Grauls G, Mariman EC, Wouters EFM, Savelkoul PH, Lopez-Iglesias C, Koenen RR, Rohde GGU, Stassen FRM
Journal
J Cell Sci
Abstract
Appropriate isolation methods are essential for unravelling the relative contribution of extracellul (show more...)
EV-METRIC
62% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration SEC UF Protein markers
EV: CD81/ CD63/ CD9/ MFGE8
non-EV: None Proteomics
yes
Show all info
Study aim
Function, Identification of content (omics approaches), Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
BEAS2B
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
>=18h at >= 100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Regenerated cellulose
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
0.5
Resin type
Sepharose CL-4B
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Detected EV-associated proteins
CD63, CD81, MFGE8
Flow cytometry specific beads
Selected surface protein(s)
CD9, CD63, CD81
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Median
Reported size (nm)
61.9
EV concentration
Yes
Particle yield
3.00E+01
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
Report size (nm)
25-200
|
||||||||
EV170054 | 5/5 | Homo sapiens | Urine |
(d)(U)C Filtration SEC |
Gheinani AH | 2017 | 62% | |
Study summaryFull title
All authors
Gheinani AH, Vögeli M, Baumgartner U, Vassella E, Draeger A, Burkhard FC, Monastyrskaya K
Journal
Sci Rep
Abstract
Circulating miRNAs are detected in extracellular space and body fluids such as urine. Circulating RN (show more...)
EV-METRIC
62% (91st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration SEC Protein markers
EV: TSG101/ CD81/ TSG101,CD81,CD9/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Size-exclusion chromatography
Total column volume (mL)
23
Sample volume/column (mL)
0.4
Resin type
Sepharose CL-2B
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Protein Yield (µg)
6.8
Western Blot
Detected EV-associated proteins
TSG101,CD81,CD9
Characterization: RNA analysis
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mode;mean;size range/distribution;D10;D50;D90
Reported size (nm)
106
EV concentration
Yes
Particle yield
see Fig1A
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Extra information
In the optimized protocol, the 16000g pellet was treated with DTT, pelleted and supernatant was pooled with supernatant from the 16000g step to increase yield (see figure 4)
|
||||||||
EV230813 | 4/6 | Staphylococcus aureus | S. aureus |
(d)(U)C DG Filtration UF |
Kim OY | 2017 | 58% | |
Study summaryFull title
All authors
Kim OY, Park HT, Dinh NTH, Choi SJ, Lee J, Kim JH, Lee SW, Gho YS
Journal
Nat Commun
Abstract
Gram-negative bacteria actively secrete outer membrane vesicles, spherical nano-meter-sized proteoli (show more...)
EV-METRIC
58% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Ultrafiltration Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Staphylococcus aureus
Sample Type
Cell culture supernatant
EV-producing cells
S. aureus
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
180
Pelleting: speed (g)
150000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
3
Lowest density fraction
10%
Highest density fraction
50%
Orientation
Bottom-up
Speed (g)
200000
Duration (min)
120
Fraction processing
None
Filtration steps
0.2 or 0.22 µm/ 0.45 µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Hollowfiber
Characterization: Protein analysis
Protein Concentration Method
Bradford
Proteomics database
ProteomeXchange Consortium
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV230812 | 1/14 | Streptomyces sp. Mg1 | PSK0558 |
(d)(U)C DG Filtration |
Hoefler BC | 2017 | 57% | |
Study summaryFull title
All authors
Hoefler BC, Stubbendieck RM, Josyula NK, Moisan SM, Schulze EM, Straight PD
Journal
Cell Chem Biol
Abstract
Specialized metabolites support bacterial competitive fitness as antibiotics, signals, pigments, and (show more...)
EV-METRIC
57% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Protein markers
EV: None
non-EV: None Proteomics
yes
EV density (g/ml)
not specified
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Streptomyces sp. Mg1
Sample Type
Cell culture supernatant
EV-producing cells
PSK0558
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
235000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
8
Lowest density fraction
10%
Highest density fraction
50%
Total gradient volume, incl. sample (mL)
14
Sample volume (mL)
1
Orientation
Bottom-up
Speed (g)
100400
Duration (min)
960
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
4mL
Pelleting: speed (g)
120000
Pelleting: adjusted k-factor
1.857
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
EM
EM-type
Transmission-EM
Image type
Wide-field
Extra information
TEM was performed for the time course experiment as well, but no images in paper
|
||||||||
EV230812 | 4/14 | Streptomyces sp. Mg1 | PSK0558 |
(d)(U)C DG Filtration |
Hoefler BC | 2017 | 57% | |
Study summaryFull title
All authors
Hoefler BC, Stubbendieck RM, Josyula NK, Moisan SM, Schulze EM, Straight PD
Journal
Cell Chem Biol
Abstract
Specialized metabolites support bacterial competitive fitness as antibiotics, signals, pigments, and (show more...)
EV-METRIC
57% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
WT 24h
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Protein markers
EV: None
non-EV: None Proteomics
yes
EV density (g/ml)
not specified
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Streptomyces sp. Mg1
Sample Type
Cell culture supernatant
EV-producing cells
PSK0558
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
235000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
6
Lowest density fraction
0%
Highest density fraction
50%
Total gradient volume, incl. sample (mL)
2.1
Sample volume (mL)
0.2
Orientation
Bottom-up
Speed (g)
120000
Duration (min)
1020
Fraction volume (mL)
0.2
Fraction processing
Centrifugation
Pelleting: volume per fraction
2.1
Pelleting: speed (g)
120000
Pelleting: adjusted k-factor
TDB
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV230812 | 5/14 | Streptomyces sp. Mg1 | PSK0558 |
(d)(U)C DG Filtration |
Hoefler BC | 2017 | 57% | |
Study summaryFull title
All authors
Hoefler BC, Stubbendieck RM, Josyula NK, Moisan SM, Schulze EM, Straight PD
Journal
Cell Chem Biol
Abstract
Specialized metabolites support bacterial competitive fitness as antibiotics, signals, pigments, and (show more...)
EV-METRIC
57% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
WT 36h
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Protein markers
EV: None
non-EV: None Proteomics
yes
EV density (g/ml)
not specified
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Streptomyces sp. Mg1
Sample Type
Cell culture supernatant
EV-producing cells
PSK0558
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
235000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
6
Lowest density fraction
0%
Highest density fraction
50%
Total gradient volume, incl. sample (mL)
2.1
Sample volume (mL)
0.2
Orientation
Bottom-up
Speed (g)
120000
Duration (min)
1020
Fraction volume (mL)
0.2
Fraction processing
Centrifugation
Pelleting: volume per fraction
2.1
Pelleting: speed (g)
120000
Pelleting: adjusted k-factor
TDB
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV230812 | 6/14 | Streptomyces sp. Mg1 | PSK0558 |
(d)(U)C DG Filtration |
Hoefler BC | 2017 | 57% | |
Study summaryFull title
All authors
Hoefler BC, Stubbendieck RM, Josyula NK, Moisan SM, Schulze EM, Straight PD
Journal
Cell Chem Biol
Abstract
Specialized metabolites support bacterial competitive fitness as antibiotics, signals, pigments, and (show more...)
EV-METRIC
57% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
WT 48h
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Protein markers
EV: None
non-EV: None Proteomics
yes
EV density (g/ml)
not specified
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Streptomyces sp. Mg1
Sample Type
Cell culture supernatant
EV-producing cells
PSK0558
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
235000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
6
Lowest density fraction
0%
Highest density fraction
50%
Total gradient volume, incl. sample (mL)
2.1
Sample volume (mL)
0.2
Orientation
Bottom-up
Speed (g)
120000
Duration (min)
1020
Fraction volume (mL)
0.2
Fraction processing
Centrifugation
Pelleting: volume per fraction
2.1
Pelleting: speed (g)
120000
Pelleting: adjusted k-factor
TDB
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV230812 | 7/14 | Streptomyces sp. Mg1 | PSK0558 |
(d)(U)C DG Filtration |
Hoefler BC | 2017 | 57% | |
Study summaryFull title
All authors
Hoefler BC, Stubbendieck RM, Josyula NK, Moisan SM, Schulze EM, Straight PD
Journal
Cell Chem Biol
Abstract
Specialized metabolites support bacterial competitive fitness as antibiotics, signals, pigments, and (show more...)
EV-METRIC
57% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
WT 72h
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Protein markers
EV: None
non-EV: None Proteomics
yes
EV density (g/ml)
not specified
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Streptomyces sp. Mg1
Sample Type
Cell culture supernatant
EV-producing cells
PSK0558
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
235000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
6
Lowest density fraction
0%
Highest density fraction
50%
Total gradient volume, incl. sample (mL)
2.1
Sample volume (mL)
0.2
Orientation
Bottom-up
Speed (g)
120000
Duration (min)
1020
Fraction volume (mL)
0.2
Fraction processing
Centrifugation
Pelleting: volume per fraction
2.1
Pelleting: speed (g)
120000
Pelleting: adjusted k-factor
TDB
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV230812 | 8/14 | Streptomyces sp. Mg1 | PSK0558 |
(d)(U)C DG Filtration |
Hoefler BC | 2017 | 57% | |
Study summaryFull title
All authors
Hoefler BC, Stubbendieck RM, Josyula NK, Moisan SM, Schulze EM, Straight PD
Journal
Cell Chem Biol
Abstract
Specialized metabolites support bacterial competitive fitness as antibiotics, signals, pigments, and (show more...)
EV-METRIC
57% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
WT 96h
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Protein markers
EV: None
non-EV: None Proteomics
yes
EV density (g/ml)
not specified
Show all info
Study aim
Biogenesis/cargo sorting/Identification of content (omics approaches)
Sample
Species
Streptomyces sp. Mg1
Sample Type
Cell culture supernatant
EV-producing cells
PSK0558
EV-harvesting Medium
Serum free medium
Separation Method
|