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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV230783	1/1	Salmonella enterica	chi 3744	(d)(U)C DG Filtration	Liu Q	2017	67%
Study summary Full title Outer Membrane Vesicles Derived from Enteritidis Protect against the Virulent Wild-Type Strain Infection in a Mouse Model. All authors Liu Q, Yi J, Liang K, Zhang X, Liu Q Journal J Microbiol Biotechnol Abstract Foodborne contamination and salmonellosis caused by Enteritidis (. Enteritidis) are a significant t (show more...)Foodborne contamination and salmonellosis caused by Enteritidis (. Enteritidis) are a significant threat to human health and poultry enterprises. Outer membrane vesicles (OMVs), which are naturally secreted by gram-negative bacteria, could be a good vaccine option because they have many biologically active substances, including lipopolysaccharides (LPS), outer membrane proteins (OMPs), and phospholipids, as well as periplasmic components. In the present study, we purified OMVs derived from . Enteritidis and analyzed their characteristics through silver staining and sodium dodecyl sulfate polyacrylamide gel electrophoresis. In total, 108 proteins were identified in . Enteritidis OMVs through liquid chromatography tandem mass spectrometry analysis, and OMPs, periplasmic proteins, and extracellular proteins (49.9% of total proteins) were found to be enriched in the OMVs compared with bacterial cells. Furthermore, native OMVs used in immunizations by either the intranasal route or the intraperitoneal route could elicit significant humoral and mucosal immune responses and provide strong protective efficiency against a lethal dose (~100-fold LD) of the wild-type . Enteritidis infection. These results indicated that . Enteritidis OMVs might be an ideal vaccine strategy for preventing . Enteritidis diseases. (hide) EV-METRIC 67% (94th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Cell culture supernatant Sample origin Control condition Focus vesicles outer membrane vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (Differential) (ultra)centrifugation Density gradient Filtration Protein markers EV: None non-EV: SopD/ TufA/ SEN0876 Proteomics yes Show all info Study aim Function Sample Species Salmonella enterica Sample Type Cell culture supernatant EV-producing cells chi 3744 EV-harvesting Medium Serum free medium Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 10,000 g and 50,000 g Pelleting performed Yes Pelleting: time(min) 60 Pelleting: speed (g) 40000 Density gradient Type Discontinuous Lowest density fraction 20% Highest density fraction 45% Speed (g) 150000 Duration (min) overnight Fraction processing Centrifugation Pelleting: speed (g) 40000 Filtration steps Between 0.22 and 0.45 µm Characterization: Protein analysis Protein Concentration Method BCA Proteomics database No Detected contaminants SopD/ TufA/ SEN0876 Characterization: Lipid analysis Yes

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EV-TRACK ID	EV230783
species	Salmonella enterica
sample type	Cell culture
cell type	chi 3744
condition	Control condition
separation protocol	dUC/ Density gradient/ Filtration
Exp. nr.	1
EV-METRIC %	67