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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV230753	1/1	Aggregatibacter actinomycetemcomitans	173	(d)(U)C DG Filtration	Kieselbach T	2017	72%
Study summary Full title Dataset of the proteome of purified outer membrane vesicles from the human pathogen . All authors Kieselbach T, Oscarsson J Journal Data Brief Abstract The Gram-negative bacterium is an oral and systemic pathogen, which is linked to aggressive forms o (show more...)The Gram-negative bacterium is an oral and systemic pathogen, which is linked to aggressive forms of periodontitis and can be associated with endocarditis. The outer membrane vesicles (OMVs) of this species contain effector proteins such as cytolethal distending toxin (CDT) and leukotoxin (LtxA), which they can deliver into human host cells. The OMVs can also activate innate immunity through NOD1- and NOD2-active pathogen-associated molecular patterns. This dataset provides a proteome of highly purified OMVs from serotype strain 173. The experimental data do not only include the raw data of the LC-MS/MS analysis of four independent preparations of purified OMVs but also the mass lists of the processed data and the Mascot.dat files from the database searches. In total 501 proteins are identified, of which 151 are detected in at least three of four independent preparations. In addition, this dataset contains the COG definitions and the predicted subcellular locations (PSORTb 3.0) for the entire genome of serotype strain SC1083, which is used for the evaluation of the LC-MS/MS data. These data are deposited in ProteomeXchange in the public dataset PXD002509. In addition, a scientific interpretation of this dataset by Kieselbach et al. (2015) [2] is available at http://dx.doi.org/10.1371/journal.pone.0138591. (hide) EV-METRIC 72% (96th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Cell culture supernatant Sample origin Control condition Focus vesicles outer membrane vesicles Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (Differential) (ultra)centrifugation Density gradient Filtration Adj. k-factor 15195 (pelleting) / 0 (washing) Protein markers EV: None non-EV: None Proteomics yes EV density (g/ml) not specified Show all info Study aim Identification of content (omics approaches) Sample Species Aggregatibacter actinomycetemcomitans Sample Type Cell culture supernatant EV-producing cells 173 Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed Yes Pelleting: time(min) 120 Pelleting: rotor type Type 70 Ti Pelleting: speed (g) 85 000 Pelleting: adjusted k-factor 15195 Wash: volume per pellet (ml) not specified Wash: time (min) 120 Wash: speed (g) 85000 Wash: adjusted k-factor TDB Density gradient Type Discontinuous Number of initial discontinuous layers 7 Lowest density fraction 10% Highest density fraction 45% Total gradient volume, incl. sample (mL) 4,17 Sample volume (mL) 0,15 Orientation Bottom-up Speed (g) 180000 Duration (min) 180 Fraction volume (mL) 0,2 Fraction processing Centrifugation Pelleting: volume per fraction not spec Pelleting: speed (g) 16000 Pelleting: adjusted k-factor 95526 Pelleting-wash: volume per pellet (mL) not spec Pelleting-wash: speed (g) JA-18.1 Filtration steps 0.2 or 0.22 µm Characterization: Protein analysis Protein Concentration Method Not determined Proteomics database ProteomeXchange Characterization: Lipid analysis No Characterization: Particle analysis EM EM-type Atomic force microscopy

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EV-TRACK ID	EV230753
species	Aggregatibacter actinomycetemcomitans
sample type	Cell culture
cell type	173
condition	Control condition
separation protocol	dUC/ Density gradient/ Filtration
Exp. nr.	1
EV-METRIC %	72