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You searched for: 2012 (Year of publication)
Showing 51 - 100 of 268
Showing 51 - 100 of 268
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120010 | 1/6 | Homo sapiens | Urine |
(d)(U)C UF |
Alvarez ML | 2012 | 38% | |
Study summaryFull title
All authors
Alvarez ML, Khosroheidari M, Kanchi Ravi R, DiStefano JK
Journal
Kidney Int
Abstract
Urinary exosomes are 40-100 nm vesicles containing protein, mRNA, and microRNA that may serve as bio (show more...)
EV-METRIC
38% (73rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV: Alix/ TSG101/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD9/ TSG101
ELISA
Detected EV-associated proteins
Alix/ CD9/ TSG101
|
||||||||
EV120010 | 4/6 | Homo sapiens | Urine |
(d)(U)C ExoQuick |
Alvarez ML | 2012 | 38% | |
Study summaryFull title
All authors
Alvarez ML, Khosroheidari M, Kanchi Ravi R, DiStefano JK
Journal
Kidney Int
Abstract
Urinary exosomes are 40-100 nm vesicles containing protein, mRNA, and microRNA that may serve as bio (show more...)
EV-METRIC
38% (73rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Protein markers
EV: Alix/ TSG101/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD9/ TSG101
ELISA
Detected EV-associated proteins
Alix/ CD9/ TSG101
|
||||||||
EV120010 | 5/6 | Homo sapiens | Urine |
(d)(U)C ExoQuick |
Alvarez ML | 2012 | 38% | |
Study summaryFull title
All authors
Alvarez ML, Khosroheidari M, Kanchi Ravi R, DiStefano JK
Journal
Kidney Int
Abstract
Urinary exosomes are 40-100 nm vesicles containing protein, mRNA, and microRNA that may serve as bio (show more...)
EV-METRIC
38% (73rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Protein markers
EV: Alix/ TSG101/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD9/ TSG101
ELISA
Detected EV-associated proteins
Alix/ CD9/ TSG101
|
||||||||
EV120025 | 3/3 | Homo sapiens | Semen |
(d)(U)C DG SEC |
Aalberts M | 2012 | 38% | |
Study summaryFull title
All authors
Aalberts M, van Dissel-Emiliani FM, van Adrichem NP, van Wijnen M, Wauben MH, Stout TA, Stoorvogel W
Journal
Biol Reprod
Abstract
In addition to sperm cells, seminal fluid contains various small membranous vesicles. These include (show more...)
EV-METRIC
38% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Semen
Sample origin
NAY
Focus vesicles
Prostasomes
Separation protocol
Separation protocol
(d)(U)C
DG SEC Protein markers
EV: CD9
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.20
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Semen
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.4
Highest density fraction
2.5
Orientation
Top-down
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD9
|
||||||||
EV120111 | 1/3 | Bos bovis | Milk |
(d)(U)C DG UF |
Yamada T | 2012 | 33% | |
Study summaryFull title
All authors
Yamada T, Inoshima Y, Matsuda T, Ishiguro N
Journal
J Vet Med Sci
Abstract
Four methods were evaluated for isolating exosomes from bovine milk: (1) ExoQuick precipitation, (2) (show more...)
EV-METRIC
33% (46th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG UF Adj. k-factor
255.8 (pelleting)
Protein markers
EV: CD9/ MFGE8
non-EV: Proteomics
no
EV density (g/ml)
1.1-1.19
Show all info
Study aim
Technical
Sample
Species
Bos bovis
Sample Type
Milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW41
Pelleting: adjusted k-factor
255.8
Wash: volume per pellet (ml)
10
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
10
Highest density fraction
40
Orientation
Top-down
Rotor type
MLA55
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD9/ MFGE8
ELISA
Detected EV-associated proteins
MFGE8
Characterization: Particle analysis
EM
EM-type
transmission EM
|
||||||||
EV120048 | 1/1 | Rattus norvegicus/rattus | NAY |
(d)(U)C DG Filtration |
Xin H | 2012 | 33% | |
Study summaryFull title
All authors
Xin H, Li Y, Buller B, Katakowski M, Zhang Y, Wang X, Shang X, Zhang ZG, Chopp M
Journal
Stem Cells
Abstract
Multipotent mesenchymal stromal cells (MSCs) have potential therapeutic benefit for the treatment of (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV: Alix
non-EV: Proteomics
no
EV density (g/ml)
1.19-1.22
Show all info
Study aim
Function
Sample
Species
Rattus norvegicus/rattus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
180
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Top-down
Speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120024 | 2/3 | Homo sapiens | NAY |
(d)(U)C Filtration |
Tauro BJ | 2012 | 33% | |
Study summaryFull title
All authors
Tauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, Simpson RJ
Journal
Methods
Abstract
Exosomes are 40-100nm extracellular vesicles that are released from a multitude of cell types, and p (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
90.8 (pelleting)
Protein markers
EV: Alix/ HSP70/ TSG101
non-EV: Proteomics
yes
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
TLA45
Pelleting: adjusted k-factor
90.8
Wash: volume per pellet (ml)
1
Filtration steps
0.2µm > x > 0.1µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ HSP70/ TSG101
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120084 | 1/2 | Aggregatibacter actinomycetemcomitans | Bacteria |
(d)(U)C DG Filtration |
Rompikuntal PK | 2012 | 33% | |
Study summaryFull title
All authors
Rompikuntal PK, Thay B, Khan MK, Alanko J, Penttinen AM, Asikainen S, Wai SN, Oscarsson J
Journal
Infect Immun
Abstract
Aggregatibacter actinomycetemcomitans is implicated in aggressive forms of periodontitis. Similarly (show more...)
EV-METRIC
33% (82nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
NAY
Focus vesicles
OMV
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
184.6 (pelleting) / 184.6 (washing)
Protein markers
EV: LtxA/ CdtA/ OmpA/ CdtB
non-EV: DNaK Proteomics
no
Show all info
Study aim
Function
Sample
Species
Aggregatibacter actinomycetemcomitans
Sample Type
Bacteria
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 50,000 g and 100,000 g
Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
184.6
Wash: Rotor Type
70Ti
Wash: adjusted k-factor
184.6
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
10
Highest density fraction
45
Orientation
Bottom-up
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CdtA/ CdtB/ OmpA/ LtxA
Detected contaminants
DNaK
ELISA
Detected EV-associated proteins
CdtA/ CdtB/ OmpA/ LtxA
Characterization: Particle analysis
EM
EM-type
atomic force EM
Image type
Wide-field
|
||||||||
EV120084 | 2/2 | Aggregatibacter actinomycetemcomitans | Bacteria |
(d)(U)C DG Filtration |
Rompikuntal PK | 2012 | 33% | |
Study summaryFull title
All authors
Rompikuntal PK, Thay B, Khan MK, Alanko J, Penttinen AM, Asikainen S, Wai SN, Oscarsson J
Journal
Infect Immun
Abstract
Aggregatibacter actinomycetemcomitans is implicated in aggressive forms of periodontitis. Similarly (show more...)
EV-METRIC
33% (82nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
NAY
Focus vesicles
OMV
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
184.6 (pelleting) / 184.6 (washing)
Protein markers
EV: LtxA/ CdtA/ OmpA/ CdtB
non-EV: DNaK Proteomics
no
Show all info
Study aim
Function
Sample
Species
Aggregatibacter actinomycetemcomitans
Sample Type
Bacteria
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 50,000 g and 100,000 g
Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
184.6
Wash: Rotor Type
70Ti
Wash: adjusted k-factor
184.6
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
10
Highest density fraction
45
Orientation
Bottom-up
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CdtA/ CdtB/ OmpA/ LtxA
Detected contaminants
DNaK
ELISA
Detected EV-associated proteins
CdtA/ CdtB/ OmpA/ LtxA
Characterization: Particle analysis
EM
EM-type
atomic force EM
Image type
Wide-field
|
||||||||
EV120083 | 1/1 | Homo sapiens | Urine |
(d)(U)C DC |
Raj DA | 2012 | 33% | |
Study summaryFull title
All authors
Raj DA, Fiume I, Capasso G, Pocsfalvi G
Journal
Kidney Int
Abstract
Urinary exosomes have received considerable attention as a potential biomarker source for the diagno (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC Adj. k-factor
78.45 (pelleting)
Protein markers
EV: Alix/ TSG101/ Beta-actin/ AQP2/ CD9
non-EV: uromodulin Proteomics
yes
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
78.45
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD9/ TSG101/ AQP2/ Beta-actin
ELISA
Detected EV-associated proteins
AQP2/ Beta-actin
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120079 | 1/2 | Homo sapiens | NAY |
(d)(U)C Filtration UF |
Nabhan JF | 2012 | 33% | |
Study summaryFull title
All authors
Nabhan JF, Hu R, Oh RS, Cohen SN, Lu Q
Journal
Proc Natl Acad Sci U S A
Abstract
Mammalian cells are capable of delivering multiple types of membrane capsules extracellularly. The l (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
microvesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Adj. k-factor
213.2 (pelleting)
Protein markers
EV: TSG101/ CD63
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW41
Pelleting: adjusted k-factor
213.2
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ TSG101
Characterization: Particle analysis
None
|
||||||||
EV120079 | 2/2 | Homo sapiens | NAY |
(d)(U)C Filtration UF |
Nabhan JF | 2012 | 33% | |
Study summaryFull title
All authors
Nabhan JF, Hu R, Oh RS, Cohen SN, Lu Q
Journal
Proc Natl Acad Sci U S A
Abstract
Mammalian cells are capable of delivering multiple types of membrane capsules extracellularly. The l (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
microvesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Adj. k-factor
54.74 (pelleting)
Protein markers
EV: TSG101/ CD63
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
TLA110
Pelleting: adjusted k-factor
54.74
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ TSG101
Characterization: Particle analysis
None
|
||||||||
EV120035 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration |
Lv LH | 2012 | 33% | |
Study summaryFull title
All authors
Lv LH, Wan YL, Lin Y, Zhang W, Yang M, Li GL, Lin HM, Shang CZ, Chen YJ, Min J
Journal
J Biol Chem
Abstract
Failure of immune surveillance related to inadequate host antitumor immune responses has been sugges (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
232.5 (pelleting) / 232.5 (washing)
Protein markers
EV: CD63/ HSP90/ GAPDH/ HSP70/ AChE/ HSP60
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
180
Pelleting: rotor type
SW41
Pelleting: adjusted k-factor
232.5
Wash: Rotor Type
SW41
Wash: adjusted k-factor
232.5
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ HSP90/ HSP70/ HSP60/ GAPDH/ AChE
ELISA
Detected EV-associated proteins
HSP60/ GAPDH/ AChE
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV120071 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG Filtration |
Lee HD | 2012 | 33% | |
Study summaryFull title
All authors
Lee HD, Koo BH, Kim YH, Jeon OH, Kim DS
Journal
FASEB J
Abstract
A disintegrin and metalloproteinase 15 (ADAM15), the only ADAM protein containing an Arg-Gly-Asp (RG (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
138.6 (pelleting)
Protein markers
EV: TSG101/ CD9/ GAPDH/ ADAM15
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW55
Pelleting: adjusted k-factor
138.6
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD9/ TSG101/ GAPDH/ ADAM15
ELISA
Detected EV-associated proteins
GAPDH/ ADAM15
Characterization: Particle analysis
EM
EM-type
immune EM
EM protein
ADAM15
Image type
Close-up
|
||||||||
EV120070 | 1/1 | Mus musculus | NAY |
(d)(U)C DG |
Lee EY | 2012 | 33% | |
Study summaryFull title
All authors
Lee EY, Park KS, Yoon YJ, Lee J, Moon HG, Jang SC, Choi KH, Kim YK, Gho YS
Journal
PLoS One
Abstract
Cancer vaccines with optimal tumor-associated antigens show promise for anti-tumor immunotherapy. Re (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Nano(-sized) vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Tyrosinase/ Melan-A/ Histone
non-EV: Proteomics
no
EV density (g/ml)
1.087
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Density gradient
Lowest density fraction
5
Highest density fraction
20
Orientation
Bottom-up
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Tyrosinase/ Melan-A/ Histone
ELISA
Detected EV-associated proteins
Tyrosinase/ Melan-A/ Histone
Characterization: Particle analysis
None
|
||||||||
EV120033 | 1/1 | Drosophila melanogaster | Drosophila |
(d)(U)C DC |
Koles K | 2012 | 33% | |
Study summaryFull title
All authors
Koles K, Nunnari J, Korkut C, Barria R, Brewer C, Li Y, Leszyk J, Zhang B, Budnik V
Journal
J Biol Chem
Abstract
Wnt signaling plays critical roles during synaptic development and plasticity. However, the mechanis (show more...)
EV-METRIC
33% (12th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Drosophila
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC Protein markers
EV: Alix/ Lbm
non-EV: Proteomics
yes
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Drosophila melanogaster
Sample Type
Drosophila
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ Lbm
ELISA
Detected EV-associated proteins
Lbm
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV120021 | 1/2 | Homo sapiens | NAY | (d)(U)C | King HW | 2012 | 33% | |
Study summaryFull title
All authors
King HW, Michael MZ, Gleadle JM
Journal
BMC Cancer
Abstract
BACKGROUND: Exosomes are nanovesicles secreted by tumour cells which have roles in paracrine signall (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ Cofilin/ Flotilin1/ CD63
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ Flotilin1/ TSG101/ Cofilin
ELISA
Detected EV-associated proteins
Cofilin
Characterization: Particle analysis
NTA
EM
EM-type
immune EM
EM protein
CD63
Image type
Close-up
|
||||||||
EV120012 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration |
Chiba M | 2012 | 33% | |
Study summaryFull title
All authors
Chiba M, Kimura M, Asari S
Journal
Oncol Rep
Abstract
Exosomes are microvesicles that are released from various cells into the extracellular space. It has (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: CD81/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
180
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD81/ CD9
Characterization: Particle analysis
None
|
||||||||
EV120030 | 1/1 | Homo sapiens | Urine | (d)(U)C | Chen CL | 2012 | 33% | |
Study summaryFull title
All authors
Chen CL, Lai YF, Tang P, Chien KY, Yu JS, Tsai CH, Chen HW, Wu CC, Chung T, Hsu CW, Chen CD, Chang YS, Chang PL, Chen YT
Journal
J Proteome Res
Abstract
Bladder cancer is a common urologic cancer whose incidence continues to rise annually. Urinary micro (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
microparticles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ CD9
non-EV: Proteomics
yes
TEM measurements
30-100
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Wash: volume per pellet (ml)
5
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD9/ TSG101
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
Report size (nm)
30-100
|
||||||||
EV120020 | 1/2 | Mus musculus | NAY | (d)(U)C | Bobrie A | 2012 | 33% | |
Study summaryFull title
All authors
Bobrie A, Colombo M, Krumeich S, Raposo G, Théry C
Journal
J Extracell Vesicles
Abstract
Exosomes are extracellular vesicles of 50 to 100 nm in diameter, released by many cell types. Exosom (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ CD63/ MFGE8/ Alix/ HSP70/ CD9
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD63/ CD9/ HSP70/ TSG101/ MFGE8
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
MFGE8
Characterization: Particle analysis
EM
EM-type
immune EM
EM protein
CD9
Image type
Wide-field
|
||||||||
EV120020 | 2/2 | Mus musculus | NAY |
(d)(U)C DG |
Bobrie A | 2012 | 33% | |
Study summaryFull title
All authors
Bobrie A, Colombo M, Krumeich S, Raposo G, Théry C
Journal
J Extracell Vesicles
Abstract
Exosomes are extracellular vesicles of 50 to 100 nm in diameter, released by many cell types. Exosom (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: CD63/ CD9/ MFGE8
non-EV: Proteomics
no
EV density (g/ml)
1.09-1.14;1.26-1.29
Show all info
Study aim
Technical
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Density gradient
Lowest density fraction
0.4
Highest density fraction
2.5
Orientation
Bottom-up
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD9/ MFGE8
ELISA
Detected EV-associated proteins
MFGE8
Characterization: Particle analysis
None
|
||||||||
EV120010 | 2/6 | Homo sapiens | Urine |
(d)(U)C DC |
Alvarez ML | 2012 | 33% | |
Study summaryFull title
All authors
Alvarez ML, Khosroheidari M, Kanchi Ravi R, DiStefano JK
Journal
Kidney Int
Abstract
Urinary exosomes are 40-100 nm vesicles containing protein, mRNA, and microRNA that may serve as bio (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC Protein markers
EV: Alix/ TSG101/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ CD9/ TSG101
ELISA
Detected EV-associated proteins
Alix/ CD9/ TSG101
Characterization: Particle analysis
None
|
||||||||
EV120104 | 1/1 | Homo sapiens | Synovial fluid |
(d)(U)C Filtration |
György B | 2012 | 29% | |
Study summaryFull title
All authors
György B, Szabó TG, Turiák L, Wright M, Herczeg P, Lédeczi Z, Kittel A, Polgár A, Tóth K, Dérfalvi B, Zelenák G, Böröcz I, Carr B, Nagy G, Vékey K, Gay S, Falus A, Buzás EI
Journal
PLoS One
Abstract
INTRODUCTION: Microvesicles (MVs), earlier referred to as microparticles, represent a major type of (show more...)
EV-METRIC
29% (21st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Synovial fluid
Sample origin
NAY
Focus vesicles
microvesicles / microparticles
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Synovial fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Filtration steps
> 0.45 µm,
Characterization: Protein analysis
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120170 | 1/1 | Pseudomonas aeruginosa | Bacteria |
(d)(U)C Filtration |
Wessel AK | 2012 | 29% | |
Study summaryFull title
Role of Pseudomonas aeruginosa peptidoglycan-associated outer membrane proteins in vesicle formation
All authors
Wessel AK, Liew J, Kwon T, Marcotte EM, Whiteley M
Journal
J Bacteriol
Abstract
Gram-negative bacteria produce outer membrane vesicles (OMVs) that package and deliver proteins, sma (show more...)
EV-METRIC
29% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
NAY
Focus vesicles
Vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
59.21 (pelleting)
Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Pseudomonas aeruginosa
Sample Type
Bacteria
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
59.21
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Protein analysis
Characterization: Particle analysis
None
|
||||||||
EV120095 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration |
Wahlgren J | 2012 | 29% | |
Study summaryFull title
Activated human T cells secrete exosomes that participate in IL-2 mediated immune response signaling
All authors
Wahlgren J, Karlson Tde L, Glader P, Telemo E, Valadi H
Journal
PLoS One
Abstract
It has previously been shown that nano-meter sized vesicles (30-100 nm), exosomes, secreted by antig (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
130.7 (pelleting)
Protein markers
EV: CD63/ CD81/ ICAM1/ MHC2/ CD9/ MHC1
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
130.7
Filtration steps
0.22µm or 0.2µm
Western Blot
Detected EV-associated proteins
MHC1/ MHC2/ ICAM1
ELISA
Detected EV-associated proteins
MHC1/ MHC2/ ICAM1
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
DLS
|
||||||||
EV120090 | 3/3 | Homo sapiens | Placental explant |
(d)(U)C DG Filtration |
Tolosa JM | 2012 | 29% | |
Study summaryFull title
All authors
Tolosa JM, Schjenken JE, Clifton VL, Vargas A, Barbeau B, Lowry P, Maiti K, Smith R
Journal
Placenta
Abstract
OBJECTIVES: To examine whether syncytin-1 has immune regulatory functions and is carried by human pl (show more...)
EV-METRIC
29% (37th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Placental explant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.16-1.19
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Placental explant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Density gradient
Lowest density fraction
20
Highest density fraction
60
Orientation
Top-down
Speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120088 | 1/2 | Dictyostelium discoideum | NAY | (d)(U)C | Tatischeff I | 2012 | 29% | |
Study summaryFull title
All authors
Tatischeff I, Larquet E, Falcón-Pérez JM, Turpin PY, Kruglik SG
Journal
J Extracell Vesicles
Abstract
The joint use of 3 complementary techniques, namely, nanoparticle tracking analysis (NTA), cryo-elec (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Dictyostelium discoideum
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Characterization: Particle analysis
NTA
EM
EM-type
cryo EM
Image type
Close-up, Wide-field
|
||||||||
EV120087 | 1/3 | Mus musculus | NAY |
(d)(U)C DG |
Singh PP | 2012 | 29% | |
Study summaryFull title
All authors
Singh PP, Smith VL, Karakousis PC, Schorey JS
Journal
J Immunol
Abstract
More than 2 billion people are infected with Mycobacterium. tuberculosis; however, only 5-10% of tho (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV:
non-EV: Proteomics
no
EV density (g/ml)
1.13-1.18
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Bottom-up
Speed (g)
100000
Pelleting-wash: volume per pellet (mL)
3
Characterization: Particle analysis
NTA
|
||||||||
EV120157 | 1/1 | Bartonella henselae | Bacteria |
(d)(U)C Filtration |
Roden JA | 2012 | 29% | |
Study summaryFull title
All authors
Roden JA, Wells DH, Chomel BB, Kasten RW, Koehler JE
Journal
Infect Immun
Abstract
Bartonella species are gram-negative, emerging bacterial pathogens found in two distinct environment (show more...)
EV-METRIC
29% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
NAY
Focus vesicles
OMV
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
139.8 (pelleting)
Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Other/Function of a bacterial protein in infection
Sample
Species
Bartonella henselae
Sample Type
Bacteria
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
45Ti
Pelleting: adjusted k-factor
139.8
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120038 | 1/2 | Homo sapiens | Brain tissue |
(d)(U)C Filtration |
Perez-Gonzalez R | 2012 | 29% | |
Study summaryFull title
All authors
Perez-Gonzalez R, Gauthier SA, Kumar A, Levy E
Journal
J Biol Chem
Abstract
In vitro studies have shown that neuronal cell cultures secrete exosomes containing amyloid-? precur (show more...)
EV-METRIC
29% (29th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Brain tissue
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
Brain tissue
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Wash: volume per pellet (ml)
60
Filtration steps
0.22µm or 0.2µm
Characterization: Particle analysis
EM
EM-type
immune EM
EM protein
Flotillin; Tsg101;APP;Beta-amyloid
Image type
Close-up, Wide-field
|
||||||||
EV120148 | 1/1 | Mus musculus | NAY |
(d)(U)C Filtration UF |
Munich S | 2012 | 29% | |
Study summaryFull title
All authors
Munich S, Sobo-Vujanovic A, Buchser WJ, Beer-Stolz D, Vujanovic NL
Journal
Oncoimmunology
Abstract
Autocrine and paracrine cell communication can be conveyed by multiple mediators, including membrane (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Adj. k-factor
126 (pelleting)
Protein markers
EV: MHC2/ MHC1
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
90Ti
Pelleting: adjusted k-factor
126.0
Filtration steps
0.45µm > x > 0.22µm,
Western Blot
Detected EV-associated proteins
MHC1/ MHC2
ELISA
Detected EV-associated proteins
MHC1/ MHC2
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120077 | 1/2 | Mus musculus | NAY |
(d)(U)C Filtration |
Marton A | 2012 | 29% | |
Study summaryFull title
All authors
Marton A, Vizler C, Kusz E, Temesfoi V, Szathmary Z, Nagy K, Szegletes Z, Varo G, Siklos L, Katona RL, Tubak V, Howard OM, Duda E, Minarovits J, Nagy K, Buzas K
Journal
Immunol Lett
Abstract
To clarify controversies in the literature of the field, we have purified and characterized B16F1 me (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
104.6 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
104.6
Filtration steps
0.22µm or 0.2µm
Characterization: Particle analysis
EM
EM-type
transmission EM/ atomic force EM
Image type
Close-up, Wide-field
|
||||||||
EV120077 | 2/2 | Mus musculus | NAY |
(d)(U)C Filtration |
Marton A | 2012 | 29% | |
Study summaryFull title
All authors
Marton A, Vizler C, Kusz E, Temesfoi V, Szathmary Z, Nagy K, Szegletes Z, Varo G, Siklos L, Katona RL, Tubak V, Howard OM, Duda E, Minarovits J, Nagy K, Buzas K
Journal
Immunol Lett
Abstract
To clarify controversies in the literature of the field, we have purified and characterized B16F1 me (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
81.62 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
T1270
Pelleting: adjusted k-factor
81.62
Filtration steps
0.22µm or 0.2µm
Characterization: Particle analysis
EM
EM-type
transmission EM/ atomic force EM
Image type
Close-up, Wide-field
|
||||||||
EV120063 | 1/1 | Homo sapiens | NAY | (d)(U)C | Hergenreider E | 2012 | 29% | |
Study summaryFull title
All authors
Hergenreider E, Heydt S, Tréguer K, Boettger T, Horrevoets AJ, Zeiher AM, Scheffer MP, Frangakis AS, Yin X, Mayr M, Braun T, Urbich C, Boon RA, Dimmeler S
Journal
Nat Cell Biol
Abstract
The shear-responsive transcription factor Krüppel-like factor 2 (KLF2) is a critical regulator of e (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV:
non-EV: Proteomics
yes
TEM measurements
60-130(99median)
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Protein analysis
Characterization: Particle analysis
EM
EM-type
cryo EM
Image type
Close-up
Report size (nm)
60-130(99median)
|
||||||||
EV120057 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration |
Ekström K | 2012 | 29% | |
Study summaryFull title
All authors
Ekström K, Valadi H, Sjöstrand M, Malmhäll C, Bossios A, Eldh M, Lötvall J
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Exosomes are nanosized vesicles of endocytic origin that are released into the extracell (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
130.7 (pelleting)
Protein markers
EV: CD81/ CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
130.7
Filtration steps
0.22µm or 0.2µm
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
EM
EM-type
transmission EM
|
||||||||
EV120108 | 1/2 | Mesocricetus auratus | NAY |
(d)(U)C DC |
Wik L | 2012 | 25% | |
Study summaryFull title
All authors
Wik L, Klingeborn M, Willander H, Linne T
Journal
Prion
Abstract
The cellular prion protein (PrP (C) ) is attached to the cell membrane via its glycosylphosphatidyli (show more...)
EV-METRIC
25% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
DC Protein markers
EV: TSG101/ Actin
non-EV: Proteomics
no
Show all info
Study aim
Other/The shedding and release mechanism of prion proteins
Sample
Species
Mesocricetus auratus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
TSG101/ Actin
ELISA
Detected EV-associated proteins
Actin
Characterization: Particle analysis
EM
EM-type
immune EM/ scanning EM
EM protein
PrP; BLV-gp51
Image type
Close-up, Wide-field
Report size (nm)
Not reported
|
||||||||
EV120024 | 1/3 | Homo sapiens | NAY |
(d)(U)C Filtration IAF |
Tauro BJ | 2012 | 25% | |
Study summaryFull title
All authors
Tauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, Simpson RJ
Journal
Methods
Abstract
Exosomes are 40-100nm extracellular vesicles that are released from a multitude of cell types, and p (show more...)
EV-METRIC
25% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration IAF Protein markers
EV: Alix/ HSP70/ TSG101
non-EV: Proteomics
yes
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Filtration steps
0.2µm > x > 0.1µm
Immunoaffinity capture
Selected surface protein(s)
EpCAM
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ HSP70/ TSG101
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120046 | 2/2 | Homo sapiens | NAY |
(d)(U)C ExoQuick |
Surgucheva I | 2012 | 25% | |
Study summaryFull title
All authors
Surgucheva I, Sharov VS, Surguchov A
Journal
Biochemistry
Abstract
Protein misfolding and aggregation is a ubiquitous phenomenon associated with a wide range of diseas (show more...)
EV-METRIC
25% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Protein markers
EV: CD63/ Alpha-synuclein
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ Alpha-synuclein
ELISA
Detected EV-associated proteins
Alpha-synuclein
Characterization: Particle analysis
None
|
||||||||
EV120045 | 1/1 | Homo sapiens | Urine |
(d)(U)C UF |
Sun AL | 2012 | 25% | |
Study summaryFull title
All authors
Sun AL, Deng JT, Guan GJ, Chen SH, Liu YT, Cheng J, Li ZW, Zhuang XH, Sun FD, Deng HP
Journal
Diab Vasc Dis Res
Abstract
The present study was designed to identify the changes in microvesicle-dipeptidyl peptidase-IV (DPP (show more...)
EV-METRIC
25% (56th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV: AD-1/ DPP IV
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
"AD-1/ DPP IV"
ELISA
Detected EV-associated proteins
"AD-1/ DPP IV"
|
||||||||
EV120021 | 2/2 | Homo sapiens | NAY |
(d)(U)C ExoQuick |
King HW | 2012 | 25% | |
Study summaryFull title
All authors
King HW, Michael MZ, Gleadle JM
Journal
BMC Cancer
Abstract
BACKGROUND: Exosomes are nanovesicles secreted by tumour cells which have roles in paracrine signall (show more...)
EV-METRIC
25% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Protein markers
EV: CD63/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
No
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ CD9
Characterization: Particle analysis
NTA
EM
EM-type
immune EM
EM protein
CD63
Image type
Close-up
|
||||||||
EV120067 | 1/2 | Homo sapiens | Serum | ExoQuick | Khan S | 2012 | 25% | |
Study summaryFull title
All authors
Khan S, Jutzy JM, Valenzuela MM, Turay D, Aspe JR, Ashok A, Mirshahidi S, Mercola D, Lilly MB, Wall NR
Journal
PLoS One
Abstract
BACKGROUND: Survivin is expressed in prostate cancer (PCa), and its downregulation sensitizes PCa ce (show more...)
EV-METRIC
25% (67th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: AChE/ LAMP1
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
LAMP1/ AChE
ELISA
Detected EV-associated proteins
LAMP1/ AChE
Characterization: Particle analysis
None
|
||||||||
EV120061 | 1/1 | Homo sapiens | Serum |
(d)(U)C UF |
Gercel-Taylor C | 2012 | 25% | |
Study summaryFull title
All authors
Gercel-Taylor C, Atay S, Tullis RH, Kesimer M, Taylor DD
Journal
Anal Biochem
Abstract
Cell-derived vesicles are recognized as essential components of intercellular communication, and man (show more...)
EV-METRIC
25% (67th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
Vesicles
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV: CD63
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Pelleting performed
No
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63
Characterization: Particle analysis
DLS
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120112 | 3/4 | Equus caballus | Follicular fluid |
(d)(U)C ExoQuick |
da Silveira JC | 2012 | 25% | |
Study summaryFull title
All authors
da Silveira JC, Veeramachaneni DN, Winger QA, Carnevale EM, Bouma GJ
Journal
Biol Reprod
Abstract
Proper cell communication within the ovarian follicle is critical for the growth and maturation of a (show more...)
EV-METRIC
25% (36th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Follicular fluid
Sample origin
NAY
Focus vesicles
Vesicles
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Protein markers
EV: HSP70
non-EV: Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Equus caballus
Sample Type
Follicular fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
HSP70
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120097 | 1/2 | Homo sapiens | NAY |
(d)(U)C UF |
Xiao D | 2012 | 22% | |
Study summaryFull title
All authors
Xiao D, Ohlendorf J, Chen Y, Taylor DD, Rai SN, Waigel S, Zacharias W, Hao H, McMasters KM
Journal
PLoS One
Abstract
BACKGROUND: Exosomes are small membranous vesicles secreted into body fluids by multiple cell types, (show more...)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV: CD81/ Annexin2/ Syntenin/ HSC70
non-EV: Cell organelle protein Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD81/ Syntenin/ HSC70/ Annexin2
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
HSC70/ Annexin2
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV120089 | 1/1 | Borrelia burgdorferi | Bacteria |
(d)(U)C DG Filtration |
Toledo A | 2012 | 22% | |
Study summaryFull title
All authors
Toledo A, Coleman JL, Kuhlow CJ, Crowley JT, Benach JL
Journal
Infect Immun
Abstract
The agent of Lyme disease, Borrelia burgdorferi, has a number of outer membrane proteins that are di (show more...)
EV-METRIC
22% (71st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
NAY
Focus vesicles
OMV
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV: OspA/ Enolase
non-EV: DnaK Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Borrelia burgdorferi
Sample Type
Bacteria
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
15
Highest density fraction
40
Orientation
Bottom-up
Speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
"OspA/ Enolase"
Detected contaminants
DnaK
ELISA
Detected EV-associated proteins
"OspA/ Enolase"
Characterization: Particle analysis
None
|
||||||||
EV120046 | 1/2 | Homo sapiens | NAY | (d)(U)C | Surgucheva I | 2012 | 22% | |
Study summaryFull title
All authors
Surgucheva I, Sharov VS, Surguchov A
Journal
Biochemistry
Abstract
Protein misfolding and aggregation is a ubiquitous phenomenon associated with a wide range of diseas (show more...)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD63/ Alpha-synuclein
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ Alpha-synuclein
ELISA
Detected EV-associated proteins
Alpha-synuclein
Characterization: Particle analysis
None
|
||||||||
EV120043 | 1/1 | Homo sapiens | NAY |
(d)(U)C IAF |
Soo CY | 2012 | 22% | |
Study summaryFull title
All authors
Soo CY, Song Y, Zheng Y, Campbell EC, Riches AC, Gunn-Moore F, Powis SJ
Journal
Immunology
Abstract
Nanoparticle tracking analysis permits the determination of both the size distribution and relative (show more...)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes / microvesicles
Separation protocol
Separation protocol
(d)(U)C
IAF Protein markers
EV: CD45/ Alix/ TSG101/ MHC1
non-EV: Cell organelle protein Proteomics
yes
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Immunoaffinity capture
Selected surface protein(s)
CD45
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ TSG101/ CD45/ MHC1
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
CD45/ MHC1
Characterization: Particle analysis
NTA
|
||||||||
EV120086 | 2/2 | Homo sapiens | Blood plasma |
(d)(U)C Filtration |
Silva J | 2012 | 22% | |
Study summaryFull title
All authors
Silva J, Garcia V, Rodriguez M, Compte M, Cisneros E, Veguillas P, Garcia JM, Dominguez G, Campos-Martin Y, Cuevas J, Peña C, Herrera M, Diaz R, Mohammed N, Bonilla F
Journal
Genes Chromosomes Cancer
Abstract
A significant proportion of extracellular nucleic acids in plasma circulate highly protected in tumo (show more...)
EV-METRIC
22% (49th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: EpCAM/ Flotilin1/ CD63
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD63/ Flotilin1/ EpCAM
ELISA
Detected EV-associated proteins
EpCAM
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
None
|
||||||||
EV120040 | 1/1 | Rattus norvegicus/rattus | NAY |
(d)(U)C DC |
Rana S | 2012 | 22% | |
Study summaryFull title
All authors
Rana S, Yue S, Stadel D, Zöller M
Journal
Int J Biochem Cell Biol
Abstract
Exosomes are discussed as potent therapeutics due to efficient transfer of proteins, mRNA and miRNA (show more...)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC Protein markers
EV: Clathrin/ Thrombospondin/ Clusterin/ Rab7/ Tubulin/ Tspan/ Neuropilin/ Annexin2/ HSP70/ GDF15/ CD9
non-EV: Cell organelle protein Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Rattus norvegicus/rattus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
CD9/ HSP70/ Clusterin/ GDF15/ Neuropilin/ Thrombospondin/ Rab7/ Tspan/ Annexin2/ Tubulin/ Clathrin
Detected contaminants
Cell organelle protein
ELISA
Detected EV-associated proteins
Clusterin/ GDF15/ Neuropilin/ Thrombospondin/ Rab7/ Tspan/ Annexin2/ Tubulin/ Clathrin
Flow cytometry specific beads
Selected surface protein(s)
Yes
Characterization: Particle analysis
None
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EV120009 | 1/2 | Homo sapiens | NAY | (d)(U)C | Putz U | 2012 | 22% | |
Study summaryFull title
All authors
Putz U, Howitt J, Doan A, Goh CP, Low LH, Silke J, Tan SS
Journal
Sci Signal
Abstract
Exosomes are microvesicles of endosomal origin that are secreted, and their contents (proteins, lipi (show more...)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Beta-actin/ Flotilin1
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Flotilin1/ Beta-actin
ELISA
Detected EV-associated proteins
Beta-actin
Characterization: Particle analysis
None
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