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You searched for: EV120070 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120070 | 1/1 | Mus musculus | NAY |
(d)(U)C DG |
Lee EY | 2012 | 33% | |
Study summaryFull title
All authors
Lee EY, Park KS, Yoon YJ, Lee J, Moon HG, Jang SC, Choi KH, Kim YK, Gho YS
Journal
PLoS One
Abstract
Cancer vaccines with optimal tumor-associated antigens show promise for anti-tumor immunotherapy. Re (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Nano(-sized) vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Tyrosinase/ Melan-A/ Histone
non-EV: Proteomics
no
EV density (g/ml)
1.087
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Density gradient
Lowest density fraction
5
Highest density fraction
20
Orientation
Bottom-up
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Tyrosinase/ Melan-A/ Histone
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Tyrosinase/ Melan-A/ Histone
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1 - 1 of 1 |
EV-TRACK ID | EV120070 |
---|---|
species | Mus musculus |
sample type | Cell culture |
cell type | NAY |
condition | NAY |
separation protocol | (d)(U)C DG |
Exp. nr. | 1 |
EV-METRIC % | 33 |