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You searched for: EV120025 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120025 | 2/3 | Homo sapiens | Semen |
(d)(U)C DG SEC |
Aalberts M | 2012 | 63% | |
Study summaryFull title
All authors
Aalberts M, van Dissel-Emiliani FM, van Adrichem NP, van Wijnen M, Wauben MH, Stout TA, Stoorvogel W
Journal
Biol Reprod
Abstract
In addition to sperm cells, seminal fluid contains various small membranous vesicles. These include (show more...)
EV-METRIC
63% (98th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Semen
Sample origin
NAY
Focus vesicles
Prostasomes
Separation protocol
Separation protocol
(d)(U)C
DG SEC Protein markers
EV: PSCA/ CD9
non-EV: Proteomics
yes
EV density (g/ml)
1.12-1.16;1.23-1.26
TEM measurements
56+-13 (high density);105+-25 (low density)
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Semen
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.4
Highest density fraction
2.5
Orientation
Bottom-up
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD9/ PSCA
ELISA
Antibody details provided?
No
Detected EV-associated proteins
PSCA
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM/ immune EM
EM protein
CD9; PSCA
Image type
Wide-field
Report size (nm)
56+-13 (high density);105+-25 (low density)
|
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EV120025 | 3/3 | Homo sapiens | Semen |
(d)(U)C DG SEC |
Aalberts M | 2012 | 38% | |
Study summaryFull title
All authors
Aalberts M, van Dissel-Emiliani FM, van Adrichem NP, van Wijnen M, Wauben MH, Stout TA, Stoorvogel W
Journal
Biol Reprod
Abstract
In addition to sperm cells, seminal fluid contains various small membranous vesicles. These include (show more...)
EV-METRIC
38% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Semen
Sample origin
NAY
Focus vesicles
Prostasomes
Separation protocol
Separation protocol
(d)(U)C
DG SEC Protein markers
EV: CD9
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.20
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Semen
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.4
Highest density fraction
2.5
Orientation
Top-down
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD9
Characterization: Particle analysis
None
|
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EV120025 | 1/3 | Homo sapiens | Semen | (d)(U)C | Aalberts M | 2012 | 22% | |
Study summaryFull title
All authors
Aalberts M, van Dissel-Emiliani FM, van Adrichem NP, van Wijnen M, Wauben MH, Stout TA, Stoorvogel W
Journal
Biol Reprod
Abstract
In addition to sperm cells, seminal fluid contains various small membranous vesicles. These include (show more...)
EV-METRIC
22% (46th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Semen
Sample origin
NAY
Focus vesicles
Membrane(-derived) vesicles / Prostasomes
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
89.21 (pelleting)
Protein markers
EV: PSCA/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Semen
EV-producing cells
DNF
Cell viability (%)
NA
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
75
Pelleting: rotor type
TLA55
Pelleting: adjusted k-factor
89.21
Wash: Rotor Type
Fixed angle
Characterization: Protein analysis
PMID previous EV protein analysis
DNF
Protein Yield (µg)
DNF
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD9/ PSCA
ELISA
Antibody details provided?
No
Not detected EV-associated proteins
Detected contaminants
DNF
Not detected contaminants
DNF
Flow cytometry aspecific beads
Antibody details provided?
No
Not detected EV-associated proteins
1
Not detected contaminants
DNF
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Selected surface protein(s)
DNF
Not detected EV-associated proteins
DNF
Not detected contaminants
DNF
Flow cytometry
Antibody details provided?
No
Not detected EV-associated proteins
DNF
Not detected contaminants
DNF
Fluorescent NTA
Antibody details provided?
No
Not detected EV-associated proteins
DNF
Not detected contaminants
DNF
Other 1
DNF
Not detected EV-associated proteins
DNF
Not detected contaminants
DNF
Other 2
DNF
Not detected EV-associated proteins
DNF
Not detected contaminants
DNF
Other 3
DNF
Not detected EV-associated proteins
DNF
Detected contaminants
DNF
Not detected contaminants
DNF
Characterization: RNA analysis
Database
DNF
Moment of Proteinase treatment
DNF
Proteinase type
DNF
Proteinase concentration
DNF
Moment of RNAse treatment
DNF
RNAse type
DNF
RNAse concentration
DNF
Characterization: Particle analysis
None
Other particle analysis name(1)
DNF
Report type
DNF
Report size
DNF
EV-concentration
DNF
Particle yield
DNF
Other particle analysis name(2)
DNF
Report type
DNF
Report size
DNF
EV-concentration
DNF
Particle yield
DNF
Other particle analysis name(3)
DNF
Report type
DNF
Report size
DNF
EV-concentration
DNF
Particle yield
DNF
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1 - 3 of 3 |
EV-TRACK ID | EV120025 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Semen | ||
medium | NA | ||
condition | NAY | NAY | NAY |
separation protocol | (d)(U)C DG SEC | (d)(U)C DG SEC | (d)(U)C |
vesicle related term | Prostasomes | Prostasomes | Membrane(-derived) vesicles Prostasomes |
Exp. nr. | 2 | 3 | 1 |
EV-METRIC % | 63 | 38 | 22 |