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You searched for: EV220244 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV220244 | 1/4 | Homo sapiens | Bone marrow-derived mesenchymal stem cells |
(d)(U)C Total Exosome Isolation |
Jiang L | 2020 | 50% | |
Study summaryFull title
All authors
Jiang L, Zhang Y, Liu T, Wang X, Wang H, Song H, Wang W
Journal
Biochimie
Abstract
Mesenchymal stromal cell (MSC)-derived exosome therapy has emerged as an effective therapy strategy (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Protein markers
EV: CD9/ CD63/ TSG101/ Alix/ TSG-6/ Actin beta
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Bone marrow-derived mesenchymal stem cells
EV-harvesting Medium
EV-depleted FBS
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Commercial kit
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD9/ CD63/ TSG101/ Alix/ TSG-6/ Actin beta
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
20-100
|
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EV220244 | 2/4 | Homo sapiens | Bone marrow-derived mesenchymal stem cells |
(d)(U)C Total Exosome Isolation |
Jiang L | 2020 | 25% | |
Study summaryFull title
All authors
Jiang L, Zhang Y, Liu T, Wang X, Wang H, Song H, Wang W
Journal
Biochimie
Abstract
Mesenchymal stromal cell (MSC)-derived exosome therapy has emerged as an effective therapy strategy (show more...)
EV-METRIC
25% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
TSG-6 lentiviral vector
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Protein markers
EV: TSG-6/ Actin beta
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Bone marrow-derived mesenchymal stem cells
EV-harvesting Medium
EV-depleted FBS
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Commercial kit
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
TSG-6/ Actin beta
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220244 | 3/4 | Homo sapiens | Bone marrow-derived mesenchymal stem cells |
(d)(U)C Total Exosome Isolation |
Jiang L | 2020 | 25% | |
Study summaryFull title
All authors
Jiang L, Zhang Y, Liu T, Wang X, Wang H, Song H, Wang W
Journal
Biochimie
Abstract
Mesenchymal stromal cell (MSC)-derived exosome therapy has emerged as an effective therapy strategy (show more...)
EV-METRIC
25% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control lentiviral vector
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Protein markers
EV: TSG-6/ Actin beta
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Bone marrow-derived mesenchymal stem cells
EV-harvesting Medium
EV-depleted FBS
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Commercial kit
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
TSG-6/ Actin beta
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220244 | 4/4 | Homo sapiens | Bone marrow-derived mesenchymal stem cells |
(d)(U)C Total Exosome Isolation |
Jiang L | 2020 | 25% | |
Study summaryFull title
All authors
Jiang L, Zhang Y, Liu T, Wang X, Wang H, Song H, Wang W
Journal
Biochimie
Abstract
Mesenchymal stromal cell (MSC)-derived exosome therapy has emerged as an effective therapy strategy (show more...)
EV-METRIC
25% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
shTSG-6 lentiviral vector
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Protein markers
EV: TSG-6/ Actin beta
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Bone marrow-derived mesenchymal stem cells
EV-harvesting Medium
EV-depleted FBS
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Commercial kit
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
TSG-6/ Actin beta
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
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1 - 4 of 4 |
EV-TRACK ID | EV220244 | |||
---|---|---|---|---|
species | Homo sapiens | |||
sample type | Cell culture | |||
cell type | Bone marrow-derived mesenchymal stem cells | |||
condition | Control condition | TSG-6 lentiviral vector | Control lentiviral vector | shTSG-6 lentiviral vector |
separation protocol | dUC/ Total Exosome Isolation | dUC/ Total Exosome Isolation | dUC/ Total Exosome Isolation | dUC/ Total Exosome Isolation |
Exp. nr. | 1 | 2 | 3 | 4 |
EV-METRIC % | 50 | 25 | 25 | 25 |