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You searched for: EV220115 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV220115 | 1/3 | Homo sapiens | Blood plasma | IAF | Tavoosidana G | 2011 | 0% | |
Study summaryFull title
All authors
Tavoosidana G, Ronquist G, Darmanis S, Yan J, Carlsson L, Wu D, Conze T, Ek P, Semjonow A, Eltze E, Larsson A, Landegren UD, Kamali-Moghaddam M
Journal
Proc Natl Acad Sci U S A
Abstract
Prostasomes are microvesicles (mean diameter, 150 nm) that are produced and secreted by normal and m (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
prostasome
Separation protocol
Separation protocol
Immunoaffinity capture (non-commercial)
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
CD13
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
|
||||||||
EV220115 | 2/3 | Homo sapiens | Blood plasma | IAF | Tavoosidana G | 2011 | 0% | |
Study summaryFull title
All authors
Tavoosidana G, Ronquist G, Darmanis S, Yan J, Carlsson L, Wu D, Conze T, Ek P, Semjonow A, Eltze E, Larsson A, Landegren UD, Kamali-Moghaddam M
Journal
Proc Natl Acad Sci U S A
Abstract
Prostasomes are microvesicles (mean diameter, 150 nm) that are produced and secreted by normal and m (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Prostate Cancer
Focus vesicles
prostasome
Separation protocol
Separation protocol
Immunoaffinity capture (non-commercial)
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
CD13
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
|
||||||||
EV220115 | 3/3 | Homo sapiens | Blood plasma |
(d)(U)C SEC (non-commercial) |
Tavoosidana G | 2011 | 0% | |
Study summaryFull title
All authors
Tavoosidana G, Ronquist G, Darmanis S, Yan J, Carlsson L, Wu D, Conze T, Ek P, Semjonow A, Eltze E, Larsson A, Landegren UD, Kamali-Moghaddam M
Journal
Proc Natl Acad Sci U S A
Abstract
Prostasomes are microvesicles (mean diameter, 150 nm) that are produced and secreted by normal and m (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Prostate Cancer
Focus vesicles
prostasome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Size-exclusion chromatography (non-commercial) Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Filtration steps
Equal to or above 100,000 g and below 150,000 g
Other
Name other separation method
Size-exclusion chromatography (non-commercial)
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
|
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1 - 3 of 3 |
EV-TRACK ID | EV220115 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Blood plasma | ||
condition | Control condition | Prostate Cancer | Prostate Cancer |
separation protocol | IAF capture (non-commercial) | IAF capture (non-commercial) | dUC/ Size-exclusion chromatography (non-commercial) |
Exp. nr. | 1 | 2 | 3 |
EV-METRIC % | 0 | 0 | 0 |