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You searched for: EV220111 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV220111 | 1/2 | Homo sapiens | Endothelial colony-forming cells |
(d)(U)C Filtration |
Liu W | 2018 | 44% | |
Study summaryFull title
All authors
Liu W, Zhang H, Mai J, Chen Z, Huang T, Wang S, Chen Y, Wang J
Journal
Med Sci Monit
Abstract
BACKGROUND The therapeutic potential of endothelial colony-forming cells (ECFCs) may be impaired in (show more...)
EV-METRIC
44% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Endothelial colony-forming cells
Sample origin
Normoxia
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD9/ Alix/ CD63
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Endothelial colony-forming cells
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
123000
Wash: time (min)
70
Wash: Rotor Type
SW 41 Ti
Wash: speed (g)
123000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Detected EV-associated proteins
CD9/ Alix/ CD63
Not detected contaminants
Calnexin
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
110
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report type
Not Reported
EV-concentration
No
|
||||||||
EV220111 | 2/2 | Homo sapiens | Endothelial colony-forming cells |
(d)(U)C Filtration |
Liu W | 2018 | 44% | |
Study summaryFull title
All authors
Liu W, Zhang H, Mai J, Chen Z, Huang T, Wang S, Chen Y, Wang J
Journal
Med Sci Monit
Abstract
BACKGROUND The therapeutic potential of endothelial colony-forming cells (ECFCs) may be impaired in (show more...)
EV-METRIC
44% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Endothelial colony-forming cells
Sample origin
Hypoxia
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD9/ Alix/ CD63
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Endothelial colony-forming cells
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
123000
Wash: time (min)
70
Wash: Rotor Type
SW 41 Ti
Wash: speed (g)
123000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Detected EV-associated proteins
CD9/ Alix/ CD63
Not detected contaminants
Calnexin
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
110
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report type
Not Reported
EV-concentration
No
|
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1 - 2 of 2 |
EV-TRACK ID | EV220111 | |
---|---|---|
species | Homo sapiens | |
sample type | Endothelial colony-forming cells | |
condition | Normoxia | Hypoxia |
separation protocol | dUC/ Filtration | dUC/ Filtration |
Exp. nr. | 1 | 2 |
EV-METRIC % | 44 | 44 |