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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV210403	1/1	Homo sapiens	GC1415	(d)(U)C	Stec M	2015	25%
Study summary Full title Interactions of tumour-derived micro(nano)vesicles with human gastric cancer cells. All authors Stec M, Szatanek R, Baj-Krzyworzeka M, Baran J, Zembala M, Barbasz J, Waligórska A, Dobrucki JW, Mytar B, Szczepanik A, Siedlar M, Drabik G, Urbanowicz B, Zembala M Journal J Transl Med Abstract Tumour cells release membrane micro(nano)fragments called tumour-derived microvesicles (TMV) that ar (show more...)Tumour cells release membrane micro(nano)fragments called tumour-derived microvesicles (TMV) that are believed to play an important role in cancer progression. TMV suppress/modify antitumour response of the host, but there is also some evidence for their direct interaction with cancer cells. In cancer patients TMV are present in body fluid and tumour microenvironment. The present study aimed at characterization of whole types/subpopulations, but not only exosomes, of TMV from newly established gastric cancer cell line (called GC1415) and to define their interactions with autologous cells. (hide) EV-METRIC 25% (64th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Cell culture supernatant Sample origin Control condition Focus vesicles (shedding) microvesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (Differential) (ultra)centrifugation Protein markers EV: EGFR/ CD44H/ CD44v6/ CCR6/ Her2-neu/ mucin1/ EMMPRIN/ EpCAM non-EV: None Proteomics no Show all info Study aim Function/Mechanism of uptake/transfer Sample Species Homo sapiens Sample Type Cell culture supernatant EV-producing cells GC1415 EV-harvesting Medium EV-depleted medium Preparation of EDS 1h at 50,000g/ Other preparation Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 800 g and 10,000 g Between 50,000 g and 100,000 g Pelleting performed Yes Pelleting: speed (g) 50000 Wash: time (min) 60 Wash: speed (g) 50000 Characterization: Protein analysis Protein Concentration Method Bradford Flow cytometry Antibody details provided? No Detected EV-associated proteins CD44H/ CD44v6/ CCR6/ Her2-neu/ mucin1/ EMMPRIN/ EpCAM Not detected EV-associated proteins EGFR Characterization: RNA analysis RNA analysis Type (RT)(q)PCR Database No Proteinase treatment No RNAse treatment No Characterization: Lipid analysis No Characterization: Particle analysis DLS Report type Modus NTA Report type Modus Reported size (nm) 120 EM EM-type Transmission-EM/ Atomic force microscopy Image type Wide-field

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EV-TRACK ID	EV210403
species	Homo sapiens
sample type	Cell culture
cell type	GC1415
condition	Control condition
separation protocol	dUC
Exp. nr.	1
EV-METRIC %	25