Search > Results
You searched for: EV210366 (EV-TRACK ID)
Showing 1 - 6 of 6
Showing 1 - 6 of 6
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210366 | 1/6 | Homo sapiens | Primary Lymphatic Endothelial Cells |
(d)(U)C ExoQuick Filtration |
Brown M | 2018 | 50% | |
Study summaryFull title
All authors
Brown M, Johnson LA, Leone DA, Majek P, Vaahtomeri K, Senfter D, Bukosza N, Schachner H, Asfour G, Langer B, Hauschild R, Parapatics K, Hong YK, Bennett KL, Kain R, Detmar M, Sixt M, Jackson DG, Kerjaschki D
Journal
J Cell Biol
Abstract
Lymphatic endothelial cells (LECs) release extracellular chemokines to guide the migration of dendri (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Exosome-rich endothelial vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Filtration Protein markers
EV: CX3CL1/ CD9
non-EV: None Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Primary Lymphatic Endothelial Cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CX3CL1
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
25 / 75 / 125 / 175 / 225 / 275
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
133
|
||||||||
EV210366 | 3/6 | Homo sapiens | Primary Lymphatic Endothelial Cells |
(d)(U)C ExoQuick Filtration |
Brown M | 2018 | 50% | |
Study summaryFull title
All authors
Brown M, Johnson LA, Leone DA, Majek P, Vaahtomeri K, Senfter D, Bukosza N, Schachner H, Asfour G, Langer B, Hauschild R, Parapatics K, Hong YK, Bennett KL, Kain R, Detmar M, Sixt M, Jackson DG, Kerjaschki D
Journal
J Cell Biol
Abstract
Lymphatic endothelial cells (LECs) release extracellular chemokines to guide the migration of dendri (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
TNF- containing EV harvesting media (7ng/mL)
Focus vesicles
Exosome-rich endothelial vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Filtration Protein markers
EV: CX3CL1/ CD9
non-EV: None Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Primary Lymphatic Endothelial Cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CX3CL1
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
25 / 75 / 125 / 175 / 225 / 275
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
83
|
||||||||
EV210366 | 2/6 | Homo sapiens | Primary Lymphatic Endothelial Cells |
(d)(U)C ExoQuick Filtration DG |
Brown M | 2018 | 37% | |
Study summaryFull title
All authors
Brown M, Johnson LA, Leone DA, Majek P, Vaahtomeri K, Senfter D, Bukosza N, Schachner H, Asfour G, Langer B, Hauschild R, Parapatics K, Hong YK, Bennett KL, Kain R, Detmar M, Sixt M, Jackson DG, Kerjaschki D
Journal
J Cell Biol
Abstract
Lymphatic endothelial cells (LECs) release extracellular chemokines to guide the migration of dendri (show more...)
EV-METRIC
37% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Exosome-rich endothelial vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Filtration Density gradient Protein markers
EV: CD63
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Primary Lymphatic Endothelial Cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
1
Orientation
Top-down
Rotor type
P40ST (Hitachi)
Speed (g)
100000
Duration (min)
960
Fraction volume (mL)
1
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210366 | 4/6 | Homo sapiens | Primary Lymphatic Endothelial Cells |
(d)(U)C ExoQuick Filtration DG |
Brown M | 2018 | 37% | |
Study summaryFull title
All authors
Brown M, Johnson LA, Leone DA, Majek P, Vaahtomeri K, Senfter D, Bukosza N, Schachner H, Asfour G, Langer B, Hauschild R, Parapatics K, Hong YK, Bennett KL, Kain R, Detmar M, Sixt M, Jackson DG, Kerjaschki D
Journal
J Cell Biol
Abstract
Lymphatic endothelial cells (LECs) release extracellular chemokines to guide the migration of dendri (show more...)
EV-METRIC
37% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
TNF- containing EV harvesting media (7ng/mL)
Focus vesicles
Exosome-rich endothelial vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Filtration Density gradient Protein markers
EV: CD63
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Primary Lymphatic Endothelial Cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
1
Orientation
Top-down
Rotor type
P40ST (Hitachi)
Speed (g)
100000
Duration (min)
960
Fraction volume (mL)
1
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210366 | 5/6 | Homo sapiens | Primary Lymphatic Endothelial Cells |
(d)(U)C ExoQuick Filtration No Extra Isolation steps |
Brown M | 2018 | 0% | |
Study summaryFull title
All authors
Brown M, Johnson LA, Leone DA, Majek P, Vaahtomeri K, Senfter D, Bukosza N, Schachner H, Asfour G, Langer B, Hauschild R, Parapatics K, Hong YK, Bennett KL, Kain R, Detmar M, Sixt M, Jackson DG, Kerjaschki D
Journal
J Cell Biol
Abstract
Lymphatic endothelial cells (LECs) release extracellular chemokines to guide the migration of dendri (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
GW4869-containing EV harvesting media (10M)
Focus vesicles
Exosome-rich endothelial vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Filtration No Extra Isolation steps Protein markers
EV: CD63
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Primary Lymphatic Endothelial Cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Other
Name other separation method
No Extra Isolation steps
Characterization: Protein analysis
None
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210366 | 6/6 | Homo sapiens | Primary Lymphatic Endothelial Cells |
(d)(U)C ExoQuick Filtration |
Brown M | 2018 | 0% | |
Study summaryFull title
All authors
Brown M, Johnson LA, Leone DA, Majek P, Vaahtomeri K, Senfter D, Bukosza N, Schachner H, Asfour G, Langer B, Hauschild R, Parapatics K, Hong YK, Bennett KL, Kain R, Detmar M, Sixt M, Jackson DG, Kerjaschki D
Journal
J Cell Biol
Abstract
Lymphatic endothelial cells (LECs) release extracellular chemokines to guide the migration of dendri (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
GW4869 (10M) and TNF- (7ng/mL) containing EV harvesting media
Focus vesicles
Exosome-rich endothelial vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Filtration Protein markers
EV: CD63
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Primary Lymphatic Endothelial Cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Characterization: Protein analysis
None
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
1 - 6 of 6 |
EV-TRACK ID | EV210366 | |||||
---|---|---|---|---|---|---|
species | Homo sapiens | |||||
sample type | Cell culture | |||||
cell type | Primary Lymphatic Endothelial Cells | |||||
condition | Control condition | TNF- containing EV harvesting media (7ng/mL) | Control condition | TNF- containing EV harvesting media (7ng/mL) | GW4869-containing EV harvesting media (10M) | GW4869 (10M) and TNF- (7ng/mL) containing EV harvesting media |
separation protocol | dUC/ ExoQuick/ Filtration | dUC/ ExoQuick/ Filtration | dUC/ ExoQuick/ Filtration/ Density gradient | dUC/ ExoQuick/ Filtration/ Density gradient | dUC/ ExoQuick/ Filtration/ No Extra Isolation steps | dUC/ ExoQuick/ Filtration |
Exp. nr. | 1 | 3 | 2 | 4 | 5 | 6 |
EV-METRIC % | 50 | 50 | 37 | 37 | 0 | 0 |