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You searched for: EV210349 (EV-TRACK ID)
Showing 1 - 13 of 13
Showing 1 - 13 of 13
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210349 | 2/13 | Homo sapiens | NA | (d)(U)C | Coulter ME | 2018 | 44% | |
Study summaryFull title
All authors
Coulter ME, Dorobantu CM, Lodewijk GA, Delalande F, Cianferani S, Ganesh VS, Smith RS, Lim ET, Xu CS, Pang S, Wong ET, Lidov HGW, Calicchio ML, Yang E, Gonzalez DM, Schlaeger TM, Mochida GH, Hess H, Lee WA, Lehtinen MK, Kirchhausen T, Haussler D, Jacobs FMJ, Gaudin R, Walsh CA
Journal
Cell Rep
Abstract
Endosomal sorting complex required for transport (ESCRT) complex proteins regulate biogenesis and re (show more...)
EV-METRIC
44% (77th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
NA
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: TSG101/ CD63/ CD81/ Syntenin/ CD9/ CHMP1A/ SHH
non-EV: gp96/ actin Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
NA
EV-producing cells
SVG-A
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
10000
Wash: volume per pellet (ml)
5
Wash: time (min)
40
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
10000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
SHH
Not detected EV-associated proteins
CD81/ Syntenin/ TSG101/ CD63/ CD9/ CHMP1A
Not detected contaminants
actin/ gp96
Characterization: Lipid analysis
No
|
||||||||
EV210349 | 3/13 | Homo sapiens | NA | (d)(U)C | Coulter ME | 2018 | 44% | |
Study summaryFull title
All authors
Coulter ME, Dorobantu CM, Lodewijk GA, Delalande F, Cianferani S, Ganesh VS, Smith RS, Lim ET, Xu CS, Pang S, Wong ET, Lidov HGW, Calicchio ML, Yang E, Gonzalez DM, Schlaeger TM, Mochida GH, Hess H, Lee WA, Lehtinen MK, Kirchhausen T, Haussler D, Jacobs FMJ, Gaudin R, Walsh CA
Journal
Cell Rep
Abstract
Endosomal sorting complex required for transport (ESCRT) complex proteins regulate biogenesis and re (show more...)
EV-METRIC
44% (77th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
NA
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: TSG101/ CD63/ CD81/ Syntenin/ CD9/ CHMP1A/ RAB18/ AXL/ TMED10
non-EV: gp96/ actin Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
NA
EV-producing cells
SVG-A
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
5
Wash: time (min)
90
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
SHH/ CD81/ Syntenin/ TSG101/ CD63/ CD9
Not detected EV-associated proteins
CHMP1A/ RAB18/ AXL/ TMED10
Not detected contaminants
actin/ gp96
Characterization: Lipid analysis
No
|
||||||||
EV210349 | 9/13 | Homo sapiens | NA | (d)(U)C | Coulter ME | 2018 | 44% | |
Study summaryFull title
All authors
Coulter ME, Dorobantu CM, Lodewijk GA, Delalande F, Cianferani S, Ganesh VS, Smith RS, Lim ET, Xu CS, Pang S, Wong ET, Lidov HGW, Calicchio ML, Yang E, Gonzalez DM, Schlaeger TM, Mochida GH, Hess H, Lee WA, Lehtinen MK, Kirchhausen T, Haussler D, Jacobs FMJ, Gaudin R, Walsh CA
Journal
Cell Rep
Abstract
Endosomal sorting complex required for transport (ESCRT) complex proteins regulate biogenesis and re (show more...)
EV-METRIC
44% (77th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
NA
Sample origin
CHMP1A null
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Syntenin/ TSG101/ CD63/ CD9/ CHMP1A/ SHH
non-EV: gp96/ actin Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
NA
EV-producing cells
SVG-A
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
10000
Wash: volume per pellet (ml)
5
Wash: time (min)
40
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
10000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
SHH
Not detected EV-associated proteins
CD81/ Syntenin/ TSG101/ CD63/ CD9/ CHMP1A
Not detected contaminants
actin/ gp96
Characterization: Lipid analysis
No
|
||||||||
EV210349 | 10/13 | Homo sapiens | NA | (d)(U)C | Coulter ME | 2018 | 44% | |
Study summaryFull title
All authors
Coulter ME, Dorobantu CM, Lodewijk GA, Delalande F, Cianferani S, Ganesh VS, Smith RS, Lim ET, Xu CS, Pang S, Wong ET, Lidov HGW, Calicchio ML, Yang E, Gonzalez DM, Schlaeger TM, Mochida GH, Hess H, Lee WA, Lehtinen MK, Kirchhausen T, Haussler D, Jacobs FMJ, Gaudin R, Walsh CA
Journal
Cell Rep
Abstract
Endosomal sorting complex required for transport (ESCRT) complex proteins regulate biogenesis and re (show more...)
EV-METRIC
44% (77th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
NA
Sample origin
CHMP1A null
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Syntenin/ TSG101/ CD63/ CD9/ CHMP1A/ SHH
non-EV: gp96/ actin Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
NA
EV-producing cells
SVG-A
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
5
Wash: time (min)
90
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
SHH
Not detected EV-associated proteins
CD81/ Syntenin/ TSG101/ CD63/ CD9/ CHMP1A
Not detected contaminants
actin/ gp96
Characterization: Lipid analysis
No
|
||||||||
EV210349 | 1/13 | Homo sapiens | NA | (d)(U)C | Coulter ME | 2018 | 33% | |
Study summaryFull title
All authors
Coulter ME, Dorobantu CM, Lodewijk GA, Delalande F, Cianferani S, Ganesh VS, Smith RS, Lim ET, Xu CS, Pang S, Wong ET, Lidov HGW, Calicchio ML, Yang E, Gonzalez DM, Schlaeger TM, Mochida GH, Hess H, Lee WA, Lehtinen MK, Kirchhausen T, Haussler D, Jacobs FMJ, Gaudin R, Walsh CA
Journal
Cell Rep
Abstract
Endosomal sorting complex required for transport (ESCRT) complex proteins regulate biogenesis and re (show more...)
EV-METRIC
33% (61st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
NA
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: TSG101/ CD63/ CD81/ Syntenin/ CD9/ CHMP1A/ SHH
non-EV: gp96/ actin Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
NA
EV-producing cells
SVG-A
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
Yes
Pelleting: speed (g)
2000
Wash: volume per pellet (ml)
5
Wash: time (min)
20
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
2000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
SHH
Not detected EV-associated proteins
CD81/ Syntenin/ TSG101/ CD63/ CD9/ CHMP1A
Not detected contaminants
actin/ gp96
Characterization: Lipid analysis
No
|
||||||||
EV210349 | 4/13 | Homo sapiens | NA |
(d)(U)C IAF |
Coulter ME | 2018 | 33% | |
Study summaryFull title
All authors
Coulter ME, Dorobantu CM, Lodewijk GA, Delalande F, Cianferani S, Ganesh VS, Smith RS, Lim ET, Xu CS, Pang S, Wong ET, Lidov HGW, Calicchio ML, Yang E, Gonzalez DM, Schlaeger TM, Mochida GH, Hess H, Lee WA, Lehtinen MK, Kirchhausen T, Haussler D, Jacobs FMJ, Gaudin R, Walsh CA
Journal
Cell Rep
Abstract
Endosomal sorting complex required for transport (ESCRT) complex proteins regulate biogenesis and re (show more...)
EV-METRIC
33% (61st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
NA
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Immunoaffinity capture (non-commercial) Protein markers
EV: CD63/ SHH/ Syntenin/ CD81/ RAB18/ AXL/ TMED10
non-EV: None Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
NA
EV-producing cells
SVG-A
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
5
Wash: time (min)
90
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Immunoaffinity capture
Selected surface protein(s)
SHH
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
SHH/ CD81/ RAB18/ AXL/ TMED10
Not detected EV-associated proteins
CD63/ Synthenin
Characterization: Lipid analysis
No
|
||||||||
EV210349 | 5/13 | Homo sapiens | NA |
(d)(U)C IAF |
Coulter ME | 2018 | 33% | |
Study summaryFull title
All authors
Coulter ME, Dorobantu CM, Lodewijk GA, Delalande F, Cianferani S, Ganesh VS, Smith RS, Lim ET, Xu CS, Pang S, Wong ET, Lidov HGW, Calicchio ML, Yang E, Gonzalez DM, Schlaeger TM, Mochida GH, Hess H, Lee WA, Lehtinen MK, Kirchhausen T, Haussler D, Jacobs FMJ, Gaudin R, Walsh CA
Journal
Cell Rep
Abstract
Endosomal sorting complex required for transport (ESCRT) complex proteins regulate biogenesis and re (show more...)
EV-METRIC
33% (61st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
NA
Sample origin
CHMP1A null
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Immunoaffinity capture (non-commercial) Protein markers
EV: CD63/ SHH/ Syntenin/ CD81/ RAB18/ AXL/ TMED10
non-EV: None Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
NA
EV-producing cells
SVG-A
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
5
Wash: time (min)
90
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Immunoaffinity capture
Selected surface protein(s)
CD63
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ SHH/ Syntenin/ CD81/ RAB18
Not detected EV-associated proteins
AXL/ TMED10
Characterization: Lipid analysis
No
|
||||||||
EV210349 | 6/13 | Homo sapiens | NA |
(d)(U)C IAF |
Coulter ME | 2018 | 33% | |
Study summaryFull title
All authors
Coulter ME, Dorobantu CM, Lodewijk GA, Delalande F, Cianferani S, Ganesh VS, Smith RS, Lim ET, Xu CS, Pang S, Wong ET, Lidov HGW, Calicchio ML, Yang E, Gonzalez DM, Schlaeger TM, Mochida GH, Hess H, Lee WA, Lehtinen MK, Kirchhausen T, Haussler D, Jacobs FMJ, Gaudin R, Walsh CA
Journal
Cell Rep
Abstract
Endosomal sorting complex required for transport (ESCRT) complex proteins regulate biogenesis and re (show more...)
EV-METRIC
33% (61st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
NA
Sample origin
CHMP1A null
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Immunoaffinity capture (non-commercial) Protein markers
EV: CD9/ CD63/ SHH
non-EV: None Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
NA
EV-producing cells
SVG-A
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
5
Wash: time (min)
90
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Immunoaffinity capture
Selected surface protein(s)
CD9
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ SHH
Characterization: Lipid analysis
No
|
||||||||
EV210349 | 7/13 | Homo sapiens | NA |
(d)(U)C IAF |
Coulter ME | 2018 | 33% | |
Study summaryFull title
All authors
Coulter ME, Dorobantu CM, Lodewijk GA, Delalande F, Cianferani S, Ganesh VS, Smith RS, Lim ET, Xu CS, Pang S, Wong ET, Lidov HGW, Calicchio ML, Yang E, Gonzalez DM, Schlaeger TM, Mochida GH, Hess H, Lee WA, Lehtinen MK, Kirchhausen T, Haussler D, Jacobs FMJ, Gaudin R, Walsh CA
Journal
Cell Rep
Abstract
Endosomal sorting complex required for transport (ESCRT) complex proteins regulate biogenesis and re (show more...)
EV-METRIC
33% (61st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
NA
Sample origin
CHMP1A null
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Immunoaffinity capture (non-commercial) Protein markers
EV: CD63/ SHH/ Syntenin/ CD81/ RAB18/ AXL/ TMED10
non-EV: None Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
NA
EV-producing cells
SVG-A
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
5
Wash: time (min)
90
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Immunoaffinity capture
Selected surface protein(s)
IgG
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ CD81
Not detected EV-associated proteins
SHH/ Synthenin/ CD81/ RAB18/ AXL/ TMED10
Characterization: Lipid analysis
No
|
||||||||
EV210349 | 8/13 | Homo sapiens | NA | (d)(U)C | Coulter ME | 2018 | 33% | |
Study summaryFull title
All authors
Coulter ME, Dorobantu CM, Lodewijk GA, Delalande F, Cianferani S, Ganesh VS, Smith RS, Lim ET, Xu CS, Pang S, Wong ET, Lidov HGW, Calicchio ML, Yang E, Gonzalez DM, Schlaeger TM, Mochida GH, Hess H, Lee WA, Lehtinen MK, Kirchhausen T, Haussler D, Jacobs FMJ, Gaudin R, Walsh CA
Journal
Cell Rep
Abstract
Endosomal sorting complex required for transport (ESCRT) complex proteins regulate biogenesis and re (show more...)
EV-METRIC
33% (61st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
NA
Sample origin
CHMP1A null
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ Syntenin/ TSG101/ CD63/ CD9/ CHMP1A/ SHH
non-EV: gp96/ actin Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
NA
EV-producing cells
SVG-A
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
Yes
Pelleting: speed (g)
2000
Wash: volume per pellet (ml)
5
Wash: time (min)
20
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
2000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Syntenin/ CD9/ CD63/ CD81/ SHH
Not detected EV-associated proteins
CHMP1A
Characterization: Lipid analysis
No
|
||||||||
EV210349 | 11/13 | Homo sapiens | CSF |
(d)(U)C IAF |
Coulter ME | 2018 | 33% | |
Study summaryFull title
All authors
Coulter ME, Dorobantu CM, Lodewijk GA, Delalande F, Cianferani S, Ganesh VS, Smith RS, Lim ET, Xu CS, Pang S, Wong ET, Lidov HGW, Calicchio ML, Yang E, Gonzalez DM, Schlaeger TM, Mochida GH, Hess H, Lee WA, Lehtinen MK, Kirchhausen T, Haussler D, Jacobs FMJ, Gaudin R, Walsh CA
Journal
Cell Rep
Abstract
Endosomal sorting complex required for transport (ESCRT) complex proteins regulate biogenesis and re (show more...)
EV-METRIC
33% (78th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
CSF
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Immunoaffinity capture (non-commercial) Protein markers
EV: SHH/ RAB18/ AXL/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
CSF
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
5
Wash: time (min)
90
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Immunoaffinity capture
Selected surface protein(s)
IgG
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63
Not detected EV-associated proteins
SHH/ RAB18/ AXL
Characterization: Lipid analysis
No
|
||||||||
EV210349 | 12/13 | Homo sapiens | CSF |
(d)(U)C IAF |
Coulter ME | 2018 | 33% | |
Study summaryFull title
All authors
Coulter ME, Dorobantu CM, Lodewijk GA, Delalande F, Cianferani S, Ganesh VS, Smith RS, Lim ET, Xu CS, Pang S, Wong ET, Lidov HGW, Calicchio ML, Yang E, Gonzalez DM, Schlaeger TM, Mochida GH, Hess H, Lee WA, Lehtinen MK, Kirchhausen T, Haussler D, Jacobs FMJ, Gaudin R, Walsh CA
Journal
Cell Rep
Abstract
Endosomal sorting complex required for transport (ESCRT) complex proteins regulate biogenesis and re (show more...)
EV-METRIC
33% (78th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
CSF
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Immunoaffinity capture (non-commercial) Protein markers
EV: SHH/ RAB18/ AXL/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
CSF
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
5
Wash: time (min)
90
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Immunoaffinity capture
Selected surface protein(s)
SHH
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ SHH/ RAB18/ AXL
Characterization: Lipid analysis
No
|
||||||||
EV210349 | 13/13 | Homo sapiens | CSF |
(d)(U)C IAF |
Coulter ME | 2018 | 33% | |
Study summaryFull title
All authors
Coulter ME, Dorobantu CM, Lodewijk GA, Delalande F, Cianferani S, Ganesh VS, Smith RS, Lim ET, Xu CS, Pang S, Wong ET, Lidov HGW, Calicchio ML, Yang E, Gonzalez DM, Schlaeger TM, Mochida GH, Hess H, Lee WA, Lehtinen MK, Kirchhausen T, Haussler D, Jacobs FMJ, Gaudin R, Walsh CA
Journal
Cell Rep
Abstract
Endosomal sorting complex required for transport (ESCRT) complex proteins regulate biogenesis and re (show more...)
EV-METRIC
33% (78th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
CSF
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Immunoaffinity capture (non-commercial) Protein markers
EV: SHH/ RAB18/ AXL/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
CSF
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
5
Wash: time (min)
90
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
100000
Immunoaffinity capture
Selected surface protein(s)
CD63
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63
Not detected EV-associated proteins
SHH/ RAB18/ AXL
Characterization: Lipid analysis
No
|
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1 - 13 of 13 |
EV-TRACK ID | EV210349 | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
species | Homo sapiens | ||||||||||||
sample type | NA | NA | NA | NA | NA | NA | NA | NA | NA | NA | CSF | CSF | CSF |
cell type | SVG-A | SVG-A | SVG-A | SVG-A | SVG-A | SVG-A | SVG-A | SVG-A | SVG-A | SVG-A | NA | NA | NA |
medium | EV-depleted medium | EV-depleted medium | EV-depleted medium | EV-depleted medium | EV-depleted medium | EV-depleted medium | EV-depleted medium | EV-depleted medium | EV-depleted medium | EV-depleted medium | NA | NA | NA |
condition | Control condition | Control condition | CHMP1A null | CHMP1A null | Control condition | Control condition | CHMP1A null | CHMP1A null | CHMP1A null | CHMP1A null | Control condition | Control condition | Control condition |
separation protocol | dUC | dUC | dUC | dUC | dUC | dUC/ IAF capture (non-commercial) | dUC/ IAF capture (non-commercial) | dUC/ IAF capture (non-commercial) | dUC/ IAF capture (non-commercial) | dUC | dUC/ IAF capture (non-commercial) | dUC/ IAF capture (non-commercial) | dUC/ IAF capture (non-commercial) |
Exp. nr. | 2 | 3 | 9 | 10 | 1 | 4 | 5 | 6 | 7 | 8 | 11 | 12 | 13 |
EV-METRIC % | 44 | 44 | 44 | 44 | 33 | 33 | 33 | 33 | 33 | 33 | 33 | 33 | 33 |