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You searched for: EV210308 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210308 | 1/1 | Homo sapiens | Raji |
(d)(U)C DC UF |
Yao Y | 2015 | 22% | |
Study summaryFull title
All authors
Yao Y, Wei W, Sun J, Chen L, Deng X, Ma L, Hao S
Journal
Eur J Med Res
Abstract
Exosomes secreted by tumor cells contain specific antigens that may have immunotherapeutic purposes. (show more...)
EV-METRIC
22% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Ultrafiltration Protein markers
EV: HSP70/ ICAM-1/ Actin
non-EV: GRP94 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Raji
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Ultra filtration
Cut-off size (kDa)
100
Membrane type
NS
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Actin/ ICAM-1/ HSP70
Detected contaminants
GRP94
Proteomics database
No
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Wide-field
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1 - 1 of 1 |
EV-TRACK ID | EV210308 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | Raji |
condition | Control condition |
separation protocol | dUC/ DC/ Ultrafiltration |
Exp. nr. | 1 |
EV-METRIC % | 22 |