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You searched for: EV210299 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210299 | 1/1 | Mus musculus | embryonic stem cells | (d)(U)C | Katsman D | 2012 | 14% | |
Study summaryFull title
All authors
Katsman D, Stackpole EJ, Domin DR, Farber DB
Journal
PLoS One
Abstract
Cell-derived microvesicles (MVs), recognized as important components of cell-cell communication, con (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Microvesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
embryonic stem cells
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Beckman Type 50.2Ti rotor
Pelleting: speed (g)
200000
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
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EV-TRACK ID | EV210299 |
---|---|
species | Mus musculus |
sample type | Cell culture |
cell type | embryonic stem cells |
condition | Control condition |
separation protocol | dUC |
Exp. nr. | 1 |
EV-METRIC % | 14 |