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You searched for: EV210246 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210246 | 1/2 | Homo sapiens | Blood plasma | (d)(U)C | Menck K | 2017 | 33% | |
Study summaryFull title
All authors
Menck K, Bleckmann A, Schulz M, Ries L, Binder C
Journal
J Vis Exp
Abstract
The release of extracellular vesicles (EVs) including small endosomal-derived exosomes (Exos, diamet (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
(shedding) microvesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD62/ CD45/ Tubulin/ CD235a/ CD9/ CD81
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
35
Pelleting: rotor type
Not specified
Pelleting: speed (g)
14000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ Tubulin/ CD81
Flow cytometry
Type of Flow cytometry
Not specified
Calibration bead size
Not specified
Antibody details provided?
No
Detected EV-associated proteins
CD62/ CD45/ CD235a
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
201
EV concentration
Yes
Particle yield
as number of particles per milliliter of starting sampl: 5.00e+0
EM
EM-type
Transmission-EM
Image type
Close-up
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EV210246 | 2/2 | Homo sapiens | Blood plasma | (d)(U)C | Menck K | 2017 | 22% | |
Study summaryFull title
All authors
Menck K, Bleckmann A, Schulz M, Ries L, Binder C
Journal
J Vis Exp
Abstract
The release of extracellular vesicles (EVs) including small endosomal-derived exosomes (Exos, diamet (show more...)
EV-METRIC
22% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD9/ CD81/ tubulin
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Not specified
Pelleting: speed (g)
110000
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD81
Not detected EV-associated proteins
Tubulin
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
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1 - 2 of 2 |
EV-TRACK ID | EV210246 | |
---|---|---|
species | Homo sapiens | |
sample type | Blood plasma | |
condition | Control condition | |
separation protocol | dUC | dUC |
vesicle related term | (shedding) microvesicle | exosome |
Exp. nr. | 1 | 2 |
EV-METRIC % | 33 | 22 |