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You searched for: EV210173 (EV-TRACK ID)
Showing 1 - 5 of 5
Showing 1 - 5 of 5
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210173 | 1/5 | Homo sapiens | U87MG |
(d)(U)C Filtration |
Wang, Huayi | 2019 | 56% | |
Study summaryFull title
All authors
Huayi Wang, Dengzhi Jiang, Wenzhe Li, Xiang Xiang, Jun Zhao, Bin Yu, Chen Wang, Zhaohui He, Ling Zhu, Yanlian Yang
Journal
Theranostics
Abstract
Rationale: Glioma is the most common malignant primary brain tumor in the central nervous system (CN (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: CD81/ Flotillin1/ EGFR
non-EV: Grp94 Proteomics
no
Show all info
Study aim
Biomarker/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
U87MG
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Not specified
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
120
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ EGFR/ CD81
Not detected contaminants
Grp94
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
EGFR
Characterization: Lipid analysis
No
|
||||||||
EV210173 | 2/5 | Homo sapiens | U251 |
(d)(U)C Filtration |
Wang, Huayi | 2019 | 56% | |
Study summaryFull title
All authors
Huayi Wang, Dengzhi Jiang, Wenzhe Li, Xiang Xiang, Jun Zhao, Bin Yu, Chen Wang, Zhaohui He, Ling Zhu, Yanlian Yang
Journal
Theranostics
Abstract
Rationale: Glioma is the most common malignant primary brain tumor in the central nervous system (CN (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: CD81/ Flotillin1/ EGFR
non-EV: Grp94 Proteomics
no
Show all info
Study aim
Biomarker/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
U251
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Not specified
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
120
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ EGFR/ CD81
Not detected contaminants
Grp94
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
EGFR
Characterization: Lipid analysis
No
|
||||||||
EV210173 | 3/5 | Homo sapiens | HA |
(d)(U)C Filtration |
Wang, Huayi | 2019 | 56% | |
Study summaryFull title
All authors
Huayi Wang, Dengzhi Jiang, Wenzhe Li, Xiang Xiang, Jun Zhao, Bin Yu, Chen Wang, Zhaohui He, Ling Zhu, Yanlian Yang
Journal
Theranostics
Abstract
Rationale: Glioma is the most common malignant primary brain tumor in the central nervous system (CN (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: CD81/ Flotillin1/ EGFR
non-EV: GRP94 Proteomics
no
Show all info
Study aim
Biomarker/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HA
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Not specified
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
120
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ CD81
Not detected EV-associated proteins
EGFR
Not detected contaminants
GRP94
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
EGFR
Characterization: Lipid analysis
No
|
||||||||
EV210173 | 5/5 | Homo sapiens | Serum |
(d)(U)C Filtration |
Wang, Huayi | 2019 | 33% | |
Study summaryFull title
All authors
Huayi Wang, Dengzhi Jiang, Wenzhe Li, Xiang Xiang, Jun Zhao, Bin Yu, Chen Wang, Zhaohui He, Ling Zhu, Yanlian Yang
Journal
Theranostics
Abstract
Rationale: Glioma is the most common malignant primary brain tumor in the central nervous system (CN (show more...)
EV-METRIC
33% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Glioma
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: EGFR/ EGFR/ FGF2/ CD81/ Flotillin-1
non-EV: HSA Proteomics
no
Show all info
Study aim
Biomarker/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
480
Pelleting: rotor type
Not specified
Pelleting: speed (g)
150000
Wash: volume per pellet (ml)
26
Wash: time (min)
120
Wash: Rotor Type
Not specified
Wash: speed (g)
150000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
EGFR/ FGF2/ CD81/ Flotillin-1
Not detected contaminants
HSA
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
EGFR
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
|
||||||||
EV210173 | 4/5 | Homo sapiens | Serum |
(d)(U)C Filtration |
Wang, Huayi | 2019 | 13% | |
Study summaryFull title
All authors
Huayi Wang, Dengzhi Jiang, Wenzhe Li, Xiang Xiang, Jun Zhao, Bin Yu, Chen Wang, Zhaohui He, Ling Zhu, Yanlian Yang
Journal
Theranostics
Abstract
Rationale: Glioma is the most common malignant primary brain tumor in the central nervous system (CN (show more...)
EV-METRIC
13% (47th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: EGFR
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
480
Pelleting: rotor type
Not specified
Pelleting: speed (g)
150000
Wash: volume per pellet (ml)
26
Wash: time (min)
120
Wash: Rotor Type
Not specified
Wash: speed (g)
150000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
EGFR
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
|
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1 - 5 of 5 |
EV-TRACK ID | EV210173 | ||||
---|---|---|---|---|---|
species | Homo sapiens | ||||
sample type | Cell culture | Cell culture | Cell culture | Serum | Serum |
cell type | U87MG | U251 | HA | NA | NA |
medium | EV-depleted medium | EV-depleted medium | EV-depleted medium | NA | NA |
condition | Control condition | Control condition | Control condition | Glioma | Control condition |
separation protocol | (d)(U)C Filtration | (d)(U)C Filtration | (d)(U)C Filtration | (d)(U)C Filtration | (d)(U)C Filtration |
Exp. nr. | 1 | 2 | 3 | 5 | 4 |
EV-METRIC % | 56 | 56 | 56 | 33 | 13 |