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You searched for: EV210032 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210032 | 1/2 | Homo sapiens | Blood plasma | (d)(U)C | Linares, Romain | 2015 | 29% | |
Study summaryFull title
All authors
Romain Linares, Sisareuth Tan, CĂ©line Gounou, Nicolas Arraud, Alain R Brisson
Journal
J Extracell Vesicles
Abstract
Plasma and other body fluids contain cell-derived extracellular vesicles (EVs), which participate in (show more...)
EV-METRIC
29% (60th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Annexin A5/ CD235a/ CD41
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
Gallios (Beckman Coulter)
Calibration bead size
0.4
Antibody details provided?
No
Detected EV-associated proteins
Annexin A5/ CD235a/ CD41
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Immuno-EM/ Cryo-EM
EM protein
Annexin A5; CD235a; CD41
Image type
Close-up, Wide-field
EV concentration
Yes
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EV-TRACK ID | EV210032 |
---|---|
species | Homo sapiens |
sample type | Blood plasma |
condition | Control condition |
separation protocol | dUC |
Exp. nr. | 1 |
EV-METRIC % | 29 |