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You searched for: EV210015 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210015 | 1/2 | Homo sapiens | Effusion |
ExoQuick Filtration dUC |
Broner, Esther Channah | 2017 | 38% | |
Study summaryFull title
All authors
Esther Channah Broner, Claes G Tropé, Reuven Reich, Ben Davidson
Journal
Hum Pathol
Abstract
The objective of this study was to analyze the expression and clinical role of molecules involved in (show more...)
EV-METRIC
38% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Effusion
Sample origin
Pleural effusion
Focus vesicles
exosome
Separation protocol
Separation protocol
Commercial method
Filtration dUC Protein markers
EV: TSAP6/ Rab27a/ nSMase2
non-EV: None Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Effusion
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Filtration steps
0.2µm > x > 0.1µm
Commercial kit
ExoQuick
Other
Name other separation method
dUC
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TSAP6/ Rab27a/ nSMase2
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Not Reported
|
||||||||
EV210015 | 2/2 | Homo sapiens | Effusion |
ExoQuick Filtration dUC |
Broner, Esther Channah | 2017 | 38% | |
Study summaryFull title
All authors
Esther Channah Broner, Claes G Tropé, Reuven Reich, Ben Davidson
Journal
Hum Pathol
Abstract
The objective of this study was to analyze the expression and clinical role of molecules involved in (show more...)
EV-METRIC
38% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Effusion
Sample origin
Peritoneal effusion
Focus vesicles
exosome
Separation protocol
Separation protocol
Commercial method
Filtration dUC Protein markers
EV: TSAP6/ Rab27a/ nSMase2
non-EV: None Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Effusion
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Filtration steps
0.2µm > x > 0.1µm
Commercial kit
ExoQuick
Other
Name other separation method
dUC
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TSAP6/ Rab27a/ nSMase2
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Not Reported
|
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1 - 2 of 2 |
EV-TRACK ID | EV210015 | |
---|---|---|
species | Homo sapiens | |
sample type | Effusion | |
condition | Pleural effusion | Peritoneal effusion |
separation protocol | ExoQuick Filtration dUC | ExoQuick Filtration dUC |
Exp. nr. | 1 | 2 |
EV-METRIC % | 38 | 38 |