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You searched for: EV200048 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200048 | 1/2 | Gallus gallus | semen |
IAF (d)(U)C ExoQuick Filtration |
Sheng Chen | 2019 | 50% | |
Study summaryFull title
All authors
Sheng Chen, Liqin Liao, Qiqi Zhao, Xinheng Zhang, Hongxin Li, Wencheng Lin, Feng Chen, Qingmei Xie
Journal
Virus Res
Abstract
MicroRNAs(miRNAs) have been reported to regulate gene expression in many processes. MiRNA in extrace (show more...)
EV-METRIC
50% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
semen
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Immunoaffinity capture (non-commercial)
(d)(U)C Commercial method Filtration Protein markers
EV: CD81/ HSP70/ TSG101/ CD63/ CD9
non-EV: GRP78 Proteomics
no
Show all info
Study aim
NA
Sample
Species
Gallus gallus
Sample Type
semen
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Immunoaffinity capture
Selected surface protein(s)
CD63
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ TSG101/ HSP70/ CD81
Not detected EV-associated proteins
HSP70/ CD81/ TSG101/ CD63/ CD9
Not detected contaminants
GRP78
ELISA
Antibody details provided?
No
Detected EV-associated proteins
HSP70/ CD63/ CD81/ CD9/ TSG101
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
110
EV concentration
Yes
Particle yield
particles/ml;Yes, other: 858000000
|
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EV200048 | 2/2 | Gallus gallus | semen |
IAF (d)(U)C ExoQuick Filtration |
Sheng Chen | 2019 | 38% | |
Study summaryFull title
All authors
Sheng Chen, Liqin Liao, Qiqi Zhao, Xinheng Zhang, Hongxin Li, Wencheng Lin, Feng Chen, Qingmei Xie
Journal
Virus Res
Abstract
MicroRNAs(miRNAs) have been reported to regulate gene expression in many processes. MiRNA in extrace (show more...)
EV-METRIC
38% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
semen
Sample origin
ALV-J infected
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Immunoaffinity capture (non-commercial)
(d)(U)C Commercial method Filtration Protein markers
EV: TSG101/ CD63/ CD81/ GRP78/ HSP70/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
NA
Sample
Species
Gallus gallus
Sample Type
semen
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Immunoaffinity capture
Selected surface protein(s)
CD63
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ TSG101/ HSP70/ CD81
Not detected EV-associated proteins
HSP70/ CD81/ GRP78/ TSG101/ CD63/ CD9
ELISA
Antibody details provided?
No
Detected EV-associated proteins
HSP70/ CD63/ CD81/ CD9/ TSG101
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;RNAsequencing
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
127
EV concentration
Yes
Particle yield
particles/ml;Yes, other: 8580000000
|
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1 - 2 of 2 |
EV-TRACK ID | EV200048 | |
---|---|---|
species | Gallus gallus | |
sample type | semen | |
condition | Control condition | ALV-J infected |
separation protocol | IAF capture (non-commercial) (d)(U)C ExoQuick Filtration | IAF capture (non-commercial) (d)(U)C ExoQuick Filtration |
Exp. nr. | 1 | 2 |
EV-METRIC % | 50 | 38 |