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You searched for: EV200011 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200011 | 1/1 | Bos taurus | milk |
(d)(U)C UF |
Lingjun, Tong | 2020 | 63% | |
Study summaryFull title
All authors
Lingjun Tong, Haining Hao, Xinyi Zhang, Zhe Zhang, Youyou Lv, Lanwei Zhang, Huaxi Yi
Journal
Mol Nutr Food Res
Abstract
Scope: Milk-derived extracellular vesicles (mEVs) as nanoparticles are being developed as novel drug (show more...)
EV-METRIC
63% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
milk
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV: TSG101/ Alix/ CD9/ CD81
non-EV: Calnexin/ Histone H3 Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Bos taurus
Sample Type
milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Between 50,000 g and 100,000 g Pelleting performed
No
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Regenerated cellulose
Characterization: Protein analysis
PMID previous EV protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ TSG101/ Alix/ CD81
Not detected contaminants
Calnexin/ Histone H3
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
CD9
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
PMID previous EV particle analysis
Extra particle analysis
NTA
Report type
Mean
Reported size (nm)
102.8
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 1.10E+10
EM
EM-type
Transmission-EM
Image type
Close-up
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1 - 1 of 1 |
EV-TRACK ID | EV200011 |
---|---|
species | Bos taurus |
sample type | milk |
condition | Control condition |
separation protocol | (d)(U)C UF |
Exp. nr. | 1 |
EV-METRIC % | 63 |