Search > Results
You searched for: EV180044 (EV-TRACK ID)
Showing 1 - 1 of 1
Showing 1 - 1 of 1
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV180044 | 1/1 | Homo sapiens | PCS-500-010 |
(d)(U)C Filtration |
Biscans A | 2018 | 55% | |
Study summaryFull title
All authors
Biscans A, Haraszti RA, Echeverria D, Miller R, Didiot MC, Nikan M, Roux L, Aronin N, Khvorova A
Journal
J Cell Sci
Abstract
Small extracellular vesicles (sEVs) show promise as natural nano-devices for delivery of therapeutic (show more...)
EV-METRIC
55% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
209.7 (pelleting) / 65.69 (washing)
Protein markers
EV: CD81/ CD63
non-EV: Calnexin Proteomics
yes
Show all info
Study aim
Mechanism of uptake/transfer, New methodological development, Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
PCS-500-010
EV-harvesting Medium
Serum free medium
Cell viability (%)
NA
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
209.7
Wash: time (min)
90
Wash: Rotor Type
TLA-110
Wash: speed (g)
100000
Wash: adjusted k-factor
65.69
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63, CD81
Not detected contaminants
Calnexin
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
140
EV concentration
Yes
Particle yield
3.20E+08 particles/million cells
EM
EM-type
Transmission-EM
Image type
Wide-field
Extra information
Rotor types and NTA data added post-publication.
|
||||||||
1 - 1 of 1 |
EV-TRACK ID | EV180044 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | PCS-500-010 |
condition | Control condition |
separation protocol | (d)(U)C Filtration |
Exp. nr. | 1 |
EV-METRIC % | 55 |