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You searched for: EV150102 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV150102 | 1/2 | Homo sapiens | TC-71, IOR/CAR | ExoQuick | Ventura S | 2015 | 25% | |
Study summaryFull title
All authors
Ventura S, Aryee DN, Felicetti F, De Feo A, Mancarella C, Manara MC, Picci P, Colombo MP, Kovar H, Carè A, Scotlandi K
Journal
Oncogenesis
Abstract
Sarcomas are mesenchymal tumors characterized by blocked differentiation process. In Ewing sarcoma ( (show more...)
EV-METRIC
25% (64th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: CD63/ CD99/ CD81/ Rab5B/ Notch1/ Notch3/ alpha-tubulin
non-EV: None Proteomics
no
Show all info
Study aim
Function, Biomarker, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
TC-71, IOR/CAR
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
>=18h at >= 100,000g
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63, CD81, Rab5B, Notch1, Notch3, CD99, alpha-tubulin
Characterization: RNA analysis
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Extra information
Publication uses exosomes to study the delivery of miRNAs from one cell type to another, and see how the cellular behaviour changes.
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EV150102 | 2/2 | Homo sapiens | TC-71, IOR/CAR | ExoQuick | Ventura S | 2015 | 25% | |
Study summaryFull title
All authors
Ventura S, Aryee DN, Felicetti F, De Feo A, Mancarella C, Manara MC, Picci P, Colombo MP, Kovar H, Carè A, Scotlandi K
Journal
Oncogenesis
Abstract
Sarcomas are mesenchymal tumors characterized by blocked differentiation process. In Ewing sarcoma ( (show more...)
EV-METRIC
25% (64th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
CD99 shRNA
Focus vesicles
exosome
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: CD81/ alpha-tubulin/ CD63/ Notch1/ Rab5B
non-EV: None Proteomics
no
Show all info
Study aim
Function, Biomarker, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
TC-71, IOR/CAR
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
>=18h at >= 100,000g
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63, CD81, Rab5B, Notch1, alpha-tubulin
Characterization: RNA analysis
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Extra information
Publication uses exosomes to study the delivery of miRNAs from one cell type to another, and see how the cellular behaviour changes.
|
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1 - 2 of 2 |
EV-TRACK ID | EV150102 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | |
cell type | TC-71 IOR/CAR | |
condition | Control condition | CD99 shRNA |
separation protocol | ExoQuick | ExoQuick |
Exp. nr. | 1 | 2 |
EV-METRIC % | 25 | 25 |