Search > Results
You searched for: EV140291 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140291 | 3/4 | Homo sapiens | NAY |
(d)(U)C ExoQuick Filtration IAF UF |
Bukong TN | 2014 | 25% | |
Study summaryFull title
All authors
Bukong TN, Momen-Heravi F, Kodys K, Bala S, Szabo G
Journal
PLoS Pathog
Abstract
Antibodies targeting receptor-mediated entry of HCV into hepatocytes confer limited therapeutic bene (show more...)
EV-METRIC
25% (64th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Filtration IAF UF Protein markers
EV: HSP90/ CD63/ Ago2
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Immunoaffinity capture
Selected surface protein(s)
CD63
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ HSP90/ Ago2
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD63/ HSP90/ Ago2
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV140291 | 4/4 | Homo sapiens | Serum |
(d)(U)C ExoQuick Filtration IAF UF |
Bukong TN | 2014 | 13% | |
Study summaryFull title
All authors
Bukong TN, Momen-Heravi F, Kodys K, Bala S, Szabo G
Journal
PLoS Pathog
Abstract
Antibodies targeting receptor-mediated entry of HCV into hepatocytes confer limited therapeutic bene (show more...)
EV-METRIC
13% (47th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Filtration IAF UF Protein markers
EV: HSP90/ CD63/ Ago2
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Immunoaffinity capture
Selected surface protein(s)
CD63
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ HSP90/ Ago2
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD63/ HSP90/ Ago2
Characterization: Particle analysis
NTA
|
||||||||
EV140291 | 1/4 | Homo sapiens | NAY |
(d)(U)C Filtration IAF UF |
Bukong TN | 2014 | 0% | |
Study summaryFull title
All authors
Bukong TN, Momen-Heravi F, Kodys K, Bala S, Szabo G
Journal
PLoS Pathog
Abstract
Antibodies targeting receptor-mediated entry of HCV into hepatocytes confer limited therapeutic bene (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration IAF UF Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Immunoaffinity capture
Selected surface protein(s)
CD63
Characterization: Particle analysis
NTA
|
||||||||
EV140291 | 2/4 | Homo sapiens | Serum |
(d)(U)C Filtration IAF UF |
Bukong TN | 2014 | 0% | |
Study summaryFull title
All authors
Bukong TN, Momen-Heravi F, Kodys K, Bala S, Szabo G
Journal
PLoS Pathog
Abstract
Antibodies targeting receptor-mediated entry of HCV into hepatocytes confer limited therapeutic bene (show more...)
EV-METRIC
0% (median: 13% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration IAF UF Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Immunoaffinity capture
Selected surface protein(s)
CD63
Characterization: Particle analysis
None
|
||||||||
1 - 4 of 4 |
EV-TRACK ID | EV140291 | |||
---|---|---|---|---|
species | Homo sapiens | |||
sample type | Cell culture | Serum | Cell culture | Serum |
cell type | NAY | NA | NAY | NA |
medium | EV Depleted | EV Depleted | ||
condition | NAY | NAY | NAY | NAY |
separation protocol | (d)(U)C ExoQuick Filtration IAF UF | (d)(U)C ExoQuick Filtration IAF UF | (d)(U)C Filtration IAF UF | (d)(U)C Filtration IAF UF |
Exp. nr. | 3 | 4 | 1 | 2 |
EV-METRIC % | 25 | 13 | 0 | 0 |