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You searched for: EV140284 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140284 | 2/2 | Homo sapiens | NAY |
(d)(U)C Filtration Microfluidics Total Exosome Isolation |
Vaidyanathan R | 2014 | 33% | |
Study summaryFull title
All authors
Vaidyanathan R, Naghibosadat M, Rauf S, Korbie D, Carrascosa LG, Shiddiky MJ, Trau M
Journal
Anal Chem
Abstract
Exosomes show promise as noninvasive biomarkers for cancer, but their effective capture and specific (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Microfluidics Total Exosome Isolation Protein markers
EV: HER2/ PSA/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
Total Exosome Isolation
Other
Name other separation method
Microfluidics
Other
Name other separation method
Total Exosome Isolation
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
HER2/ PSA
ELISA
Detected EV-associated proteins
CD9/ HER2/ PSA
Characterization: Particle analysis
DLS
TRPS
EM
EM-type
cryo EM
Image type
Close-up
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EV140284 | 1/2 | Homo sapiens | Serum |
(d)(U)C Microfluidics |
Vaidyanathan R | 2014 | 0% | |
Study summaryFull title
All authors
Vaidyanathan R, Naghibosadat M, Rauf S, Korbie D, Carrascosa LG, Shiddiky MJ, Trau M
Journal
Anal Chem
Abstract
Exosomes show promise as noninvasive biomarkers for cancer, but their effective capture and specific (show more...)
EV-METRIC
0% (median: 13% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Microfluidics Protein markers
EV: HER2/ CD9
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Other
Name other separation method
Microfluidics
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
HER2
ELISA
Detected EV-associated proteins
CD9/ HER2
Characterization: Particle analysis
None
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1 - 2 of 2 |
EV-TRACK ID | EV140284 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | Serum |
cell type | NAY | NA |
medium | serum free | |
condition | NAY | NAY |
separation protocol | (d)(U)C Filtration Microfluidics Total Exosome Isolation | (d)(U)C Microfluidics |
Exp. nr. | 2 | 1 |
EV-METRIC % | 33 | 0 |