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You searched for: EV140267 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140267 | 3/3 | Homo sapiens | NAY |
(d)(U)C Filtration |
Basu S | 2014 | 29% | |
Study summaryFull title
All authors
Basu S, Bhattacharyya SN
Journal
Nucleic Acids Res
Abstract
miRNAs are 20-22 nt long post-transcriptional regulators in metazoan cells that repress protein expr (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Filtration steps
0.22µm or 0.2µm
Characterization: Particle analysis
DLS
NTA
EM
EM-type
transmission EM/ scanning EM/ atomic force EM
Image type
Close-up, Wide-field
Report size (nm)
Not reported
|
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EV140267 | 1/3 | Homo sapiens | NAY |
(d)(U)C ExoQuick IAF |
Basu S | 2014 | 0% | |
Study summaryFull title
All authors
Basu S, Bhattacharyya SN
Journal
Nucleic Acids Res
Abstract
miRNAs are 20-22 nt long post-transcriptional regulators in metazoan cells that repress protein expr (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
ExoQuick IAF Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Commercial kit
ExoQuick
Immunoaffinity capture
Selected surface protein(s)
CD63, CD81
Other
Name other separation method
ExoQuick
Characterization: Particle analysis
None
|
||||||||
EV140267 | 2/3 | Homo sapiens | NAY |
DC Filtration |
Basu S | 2014 | 0% | |
Study summaryFull title
All authors
Basu S, Bhattacharyya SN
Journal
Nucleic Acids Res
Abstract
miRNAs are 20-22 nt long post-transcriptional regulators in metazoan cells that repress protein expr (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
DC
Filtration Protein markers
EV: Alix/ CD63
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD63
Characterization: Particle analysis
None
|
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1 - 3 of 3 |
EV-TRACK ID | EV140267 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Cell culture | ||
cell type | NAY | ||
condition | NAY | ||
separation protocol | (d)(U)C Filtration | (d)(U)C ExoQuick IAF | DC Filtration |
Exp. nr. | 3 | 1 | 2 |
EV-METRIC % | 29 | 0 | 0 |