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You searched for: EV140133 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140133 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG Filtration |
Arenaccio C | 2014 | 25% | |
Study summaryFull title
All authors
Arenaccio C, Chiozzini C, Columba-Cabezas S, Manfredi F, Affabris E, Baur A, Federico M
Journal
J Virol
Abstract
Resting CD4+ T lymphocytes resist human immunodeficiency virus (HIV) infection. Here, we provide evi (show more...)
EV-METRIC
25% (64th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV: AChE/ CD63/ ICAM1
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
6
Highest density fraction
18
Orientation
Top-down
Filtration steps
0.22µm or 0.2µm
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
AChE/ ICAM1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
AChE/ ICAM1
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1 - 1 of 1 |
EV-TRACK ID | EV140133 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | NAY |
condition | NAY |
separation protocol | (d)(U)C DG Filtration |
Exp. nr. | 1 |
EV-METRIC % | 25 |