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You searched for: EV140064 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140064 | 2/2 | Homo sapiens | Serum |
(d)(U)C ExoQuick |
Caradec J | 2014 | 25% | |
Study summaryFull title
All authors
Caradec J, Kharmate G, Hosseini-Beheshti E, Adomat H, Gleave M, Guns E
Journal
Clin Biochem
Abstract
OBJECTIVES: Exosomes are emerging as a source of biomarkers with putative prognostic and diagnostic (show more...)
EV-METRIC
25% (67th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
ExoQuick Protein markers
EV: LAMP2/ CD9
non-EV: Albumin/ HSP90B1/ Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ LAMP2
Detected contaminants
Cell organelle protein/ Albumin/ HSP90B1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
LAMP2
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Close-up
|
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EV140064 | 1/2 | Homo sapiens | Serum |
(d)(U)C DC |
Caradec J | 2014 | 22% | |
Study summaryFull title
All authors
Caradec J, Kharmate G, Hosseini-Beheshti E, Adomat H, Gleave M, Guns E
Journal
Clin Biochem
Abstract
OBJECTIVES: Exosomes are emerging as a source of biomarkers with putative prognostic and diagnostic (show more...)
EV-METRIC
22% (64th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC Protein markers
EV: LAMP2/ CD9
non-EV: Albumin/ HSP90B1/ Cell organelle protein Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ LAMP2
Detected contaminants
Cell organelle protein/ Albumin/ HSP90B1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
LAMP2
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Close-up
|
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1 - 2 of 2 |
EV-TRACK ID | EV140064 | |
---|---|---|
species | Homo sapiens | |
sample type | Serum | |
condition | NAY | |
separation protocol | (d)(U)C ExoQuick | (d)(U)C DC |
Exp. nr. | 2 | 1 |
EV-METRIC % | 25 | 22 |