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You searched for: EV140026 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140026 | 1/3 | Mus musculus | Corneal fibroblasts |
DG Filtration UF dUC |
Han KY | 2015 | 33% | |
Study summaryFull title
All authors
Han KY, Dugas-Ford J, Seiki M, Chang JH, Azar DT
Journal
Invest Ophthalmol Vis Sci
Abstract
Matrix metalloproteinase (MMP) 14 has been shown to promote angiogenesis, but the underlying mechani (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
DG
Filtration UF dUC Protein markers
EV: TSG101/ MMP14/ actin/ proMMP2/ MMP2/ ITGB1
non-EV: COX4/ MAPK Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
Corneal fibroblasts
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
120
Density gradient
Type
Continuous
Lowest density fraction
5%
Highest density fraction
40%
Orientation
Bottom-up
Rotor type
SW 40 Ti
Speed (g)
100000
Duration (min)
1080
Filtration steps
0.45µm > x > 0.22µm,
Ultra filtration
Cut-off size (kDa)
100
Membrane type
NS
Other
Name other separation method
dUC
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
MMP14/ actin/ proMMP2/ MMP2/ ITGB1/ TSG101
Detected contaminants
COX4/ MAPK
Characterization: Lipid analysis
No
|
||||||||
EV140026 | 3/3 | Mus musculus | Corneal fibroblasts |
DG Filtration UF dUC |
Han KY | 2015 | 22% | |
Study summaryFull title
All authors
Han KY, Dugas-Ford J, Seiki M, Chang JH, Azar DT
Journal
Invest Ophthalmol Vis Sci
Abstract
Matrix metalloproteinase (MMP) 14 has been shown to promote angiogenesis, but the underlying mechani (show more...)
EV-METRIC
22% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
MMP14 exon 4 deletion
Focus vesicles
USA
Separation protocol
Separation protocol
DG
Filtration UF dUC Protein markers
EV: MMP14/ proMMP2/ MMP2
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
Corneal fibroblasts
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
120
Density gradient
Type
Continuous
Lowest density fraction
5%
Highest density fraction
40%
Orientation
Bottom-up
Rotor type
SW 40 Ti
Speed (g)
100000
Duration (min)
1080
Filtration steps
0.45µm > x > 0.22µm,
Ultra filtration
Cut-off size (kDa)
100
Membrane type
NS
Other
Name other separation method
dUC
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
MMP14/ proMMP2/ MMP2
Characterization: Lipid analysis
No
|
||||||||
EV140026 | 2/3 | Mus musculus | Corneal fibroblasts |
DG Filtration UF dUC |
Han KY | 2015 | 14% | |
Study summaryFull title
All authors
Han KY, Dugas-Ford J, Seiki M, Chang JH, Azar DT
Journal
Invest Ophthalmol Vis Sci
Abstract
Matrix metalloproteinase (MMP) 14 has been shown to promote angiogenesis, but the underlying mechani (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
MMP14-YPET
Focus vesicles
exosome
Separation protocol
Separation protocol
DG
Filtration UF dUC Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
Corneal fibroblasts
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
120
Density gradient
Type
Continuous
Lowest density fraction
5%
Highest density fraction
40%
Orientation
Bottom-up
Rotor type
SW 40 Ti
Speed (g)
100000
Duration (min)
1080
Filtration steps
0.45µm > x > 0.22µm,
Ultra filtration
Cut-off size (kDa)
100
Membrane type
NS
Other
Name other separation method
dUC
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
|
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1 - 3 of 3 |
EV-TRACK ID | EV140026 | ||
---|---|---|---|
species | Mus musculus | ||
sample type | Cell culture | ||
cell type | Corneal fibroblasts | ||
condition | Control condition | MMP14 exon 4 deletion | MMP14-YPET |
separation protocol | DG Filtration UF dUC | DG Filtration UF dUC | DG Filtration UF dUC |
vesicle related term | exosome | USA | exosome |
Exp. nr. | 1 | 3 | 2 |
EV-METRIC % | 33 | 22 | 14 |