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You searched for: EV130155 (EV-TRACK ID)
Showing 1 - 5 of 5
Showing 1 - 5 of 5
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130155 | 2/5 | Homo sapiens | Saliva | IAF | Wei F | 2013 | 17% | |
Study summaryFull title
All authors
Wei F, Yang J, Wong DT
Journal
Biosens Bioelectron
Abstract
Exosomes biomarkers mediating important biological process, especially in the systemic disease diagn (show more...)
EV-METRIC
17% (40th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Saliva
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Saliva
Separation Method
Immunoaffinity capture
Selected surface protein(s)
CD63
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
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EV130155 | 1/5 | Homo sapiens | NAY | IAF | Wei F | 2013 | 0% | |
Study summaryFull title
All authors
Wei F, Yang J, Wong DT
Journal
Biosens Bioelectron
Abstract
Exosomes biomarkers mediating important biological process, especially in the systemic disease diagn (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
Immunoaffinity capture
Selected surface protein(s)
CD63
Characterization: Particle analysis
None
|
||||||||
EV130155 | 3/5 | Homo sapiens | Serum | IAF | Wei F | 2013 | 0% | |
Study summaryFull title
All authors
Wei F, Yang J, Wong DT
Journal
Biosens Bioelectron
Abstract
Exosomes biomarkers mediating important biological process, especially in the systemic disease diagn (show more...)
EV-METRIC
0% (median: 13% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
IAF
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Immunoaffinity capture
Selected surface protein(s)
CD63
Characterization: Particle analysis
None
|
||||||||
EV130155 | 4/5 | Homo sapiens | NAY |
(d)(U)C IAF |
Wei F | 2013 | 0% | |
Study summaryFull title
All authors
Wei F, Yang J, Wong DT
Journal
Biosens Bioelectron
Abstract
Exosomes biomarkers mediating important biological process, especially in the systemic disease diagn (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
IAF Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Immunoaffinity capture
Selected surface protein(s)
CD63
Characterization: Particle analysis
None
|
||||||||
EV130155 | 5/5 | Homo sapiens | Saliva |
(d)(U)C Filtration IAF |
Wei F | 2013 | 0% | |
Study summaryFull title
All authors
Wei F, Yang J, Wong DT
Journal
Biosens Bioelectron
Abstract
Exosomes biomarkers mediating important biological process, especially in the systemic disease diagn (show more...)
EV-METRIC
0% (median: 29% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Saliva
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration IAF Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Saliva
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Filtration steps
0.45µm > x > 0.22µm,
Immunoaffinity capture
Selected surface protein(s)
CD63
Characterization: Particle analysis
None
|
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1 - 5 of 5 |
EV-TRACK ID | EV130155 | ||||
---|---|---|---|---|---|
species | Homo sapiens | ||||
sample type | Saliva | Cell culture | Serum | Cell culture | Saliva |
cell type | NA | NAY | NA | NAY | NA |
condition | NAY | NAY | NAY | NAY | NAY |
separation protocol | IAF | IAF | IAF | (d)(U)C IAF | (d)(U)C Filtration IAF |
Exp. nr. | 2 | 1 | 3 | 4 | 5 |
EV-METRIC % | 17 | 0 | 0 | 0 | 0 |