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You searched for: EV130151 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130151 | 4/4 | Homo sapiens | NAY |
(d)(U)C Filtration SEC UF |
Redzic JS | 2013 | 38% | |
Study summaryFull title
All authors
Redzic JS, Kendrick AA, Bahmed K, Dahl KD, Pearson CG, Robinson WA, Robinson SE, Graner MW, Eisenmesser EZ
Journal
PLoS One
Abstract
Extracellular vesicles (EVs) are key contributors to cancer where they play an integral role in cell (show more...)
EV-METRIC
38% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration SEC UF Protein markers
EV: EMMPRIN
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
EMMPRIN
ELISA
Antibody details provided?
No
Detected EV-associated proteins
EMMPRIN
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
Report size (nm)
Not reported
|
||||||||
EV130151 | 3/4 | Homo sapiens | Serum |
(d)(U)C Filtration IAF |
Redzic JS | 2013 | 13% | |
Study summaryFull title
All authors
Redzic JS, Kendrick AA, Bahmed K, Dahl KD, Pearson CG, Robinson WA, Robinson SE, Graner MW, Eisenmesser EZ
Journal
PLoS One
Abstract
Extracellular vesicles (EVs) are key contributors to cancer where they play an integral role in cell (show more...)
EV-METRIC
13% (47th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration IAF Protein markers
EV: EMMPRIN
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Immunoaffinity capture
Selected surface protein(s)
EMMPRIN
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
EMMPRIN
ELISA
Antibody details provided?
No
Detected EV-associated proteins
EMMPRIN
Characterization: Particle analysis
None
|
||||||||
EV130151 | 1/4 | Homo sapiens | Ascites |
(d)(U)C Filtration IAF |
Redzic JS | 2013 | 0% | |
Study summaryFull title
All authors
Redzic JS, Kendrick AA, Bahmed K, Dahl KD, Pearson CG, Robinson WA, Robinson SE, Graner MW, Eisenmesser EZ
Journal
PLoS One
Abstract
Extracellular vesicles (EVs) are key contributors to cancer where they play an integral role in cell (show more...)
EV-METRIC
0% (median: 13% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Ascites
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration IAF Protein markers
EV: EMMPRIN
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Ascites
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Immunoaffinity capture
Selected surface protein(s)
EMMPRIN
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
EMMPRIN
ELISA
Antibody details provided?
No
Detected EV-associated proteins
EMMPRIN
Characterization: Particle analysis
None
|
||||||||
EV130151 | 2/4 | Homo sapiens | Blood plasma |
(d)(U)C Filtration IAF |
Redzic JS | 2013 | 0% | |
Study summaryFull title
All authors
Redzic JS, Kendrick AA, Bahmed K, Dahl KD, Pearson CG, Robinson WA, Robinson SE, Graner MW, Eisenmesser EZ
Journal
PLoS One
Abstract
Extracellular vesicles (EVs) are key contributors to cancer where they play an integral role in cell (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration IAF Protein markers
EV: EMMPRIN
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Immunoaffinity capture
Selected surface protein(s)
EMMPRIN
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
EMMPRIN
ELISA
Antibody details provided?
No
Detected EV-associated proteins
EMMPRIN
Characterization: Particle analysis
None
|
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1 - 4 of 4 |
EV-TRACK ID | EV130151 | |||
---|---|---|---|---|
species | Homo sapiens | |||
sample type | Cell culture | Serum | Ascites | Blood plasma |
cell type | NAY | NA | NA | NA |
medium | EV Depleted | |||
condition | NAY | NAY | NAY | NAY |
separation protocol | (d)(U)C Filtration SEC UF | (d)(U)C Filtration IAF | (d)(U)C Filtration IAF | (d)(U)C Filtration IAF |
Exp. nr. | 4 | 3 | 1 | 2 |
EV-METRIC % | 38 | 13 | 0 | 0 |