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You searched for: EV130118 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130118 | 1/1 | Homo sapiens | Blood plasma |
(d)(U)C Filtration |
Szajnik M | 2013 | 33% | |
Study summaryFull title
All authors
Szajnik M, Derbis M, Lach M, Patalas P, Michalak M, Drzewiecka H, Szpurek D, Nowakowski A, Spaczynski M, Baranowski W, Whiteside TL
Journal
Gynecol Obstet (Sunnyvale)
Abstract
BACKGROUND: In patients with Ovarian Cancer (OvCa) exosomes released by tumor cells are present in t (show more...)
EV-METRIC
33% (66th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
101.8 (pelleting)
Protein markers
EV: LAMP1
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70.1Ti
Pelleting: adjusted k-factor
101.8
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
LAMP1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
LAMP1
Characterization: Particle analysis
EM
EM-type
scanning EM
Image type
Wide-field
Report size (nm)
Not reported
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EV-TRACK ID | EV130118 |
---|---|
species | Homo sapiens |
sample type | Blood plasma |
condition | NAY |
separation protocol | (d)(U)C Filtration |
Exp. nr. | 1 |
EV-METRIC % | 33 |