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You searched for: EV120186 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120186 | 1/1 | Cryptococcus neoformans | Fungus |
(d)(U)C Filtration UF |
Robertson EJ | 2012 | 0% | |
Study summaryFull title
All authors
Robertson EJ, Wolf JM, Casadevall A
Journal
Appl Environ Microbiol
Abstract
The fungal pathogen Cryptococcus neoformans can grow as a biofilm on a range of synthetic and prosth (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Fungus
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Cryptococcus neoformans
Sample Type
Fungus
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Wash: volume per pellet (ml)
1
Filtration steps
> 0.45 µm,
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EV-TRACK ID | EV120186 |
---|---|
species | Cryptococcus neoformans |
sample type | Fungus |
condition | NAY |
separation protocol | (d)(U)C Filtration UF |
Exp. nr. | 1 |
EV-METRIC % | 0 |