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You searched for: EV120088 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV120088 | 1/2 | Dictyostelium discoideum | NAY | (d)(U)C | Tatischeff I | 2012 | 29% | |
Study summaryFull title
All authors
Tatischeff I, Larquet E, Falcón-Pérez JM, Turpin PY, Kruglik SG
Journal
J Extracell Vesicles
Abstract
The joint use of 3 complementary techniques, namely, nanoparticle tracking analysis (NTA), cryo-elec (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Dictyostelium discoideum
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Characterization: Particle analysis
NTA
EM
EM-type
cryo EM
Image type
Close-up, Wide-field
|
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EV120088 | 2/2 | Homo sapiens | Urine |
(d)(U)C Filtration |
Tatischeff I | 2012 | 0% | |
Study summaryFull title
All authors
Tatischeff I, Larquet E, Falcón-Pérez JM, Turpin PY, Kruglik SG
Journal
J Extracell Vesicles
Abstract
The joint use of 3 complementary techniques, namely, nanoparticle tracking analysis (NTA), cryo-elec (show more...)
EV-METRIC
0% (median: 22% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Filtration steps
0.22µm or 0.2µm
Characterization: Particle analysis
NTA
|
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1 - 2 of 2 |
EV-TRACK ID | EV120088 | |
---|---|---|
species | Dictyostelium discoideum | Homo sapiens |
sample type | Cell culture | Urine |
cell type | NAY | NA |
condition | NAY | NAY |
separation protocol | (d)(U)C | (d)(U)C Filtration |
Exp. nr. | 1 | 2 |
EV-METRIC % | 29 | 0 |