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You searched for: EV110052 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV110052 | 1/1 | Burkholderia pseudomallei | Bacteria |
(d)(U)C DG Filtration UF |
Nieves W | 2011 | 22% | |
Study summaryFull title
All authors
Nieves W, Asakrah S, Qazi O, Brown KA, Kurtz J, Aucoin DP, McLachlan JB, Roy CJ, Morici LA
Journal
Vaccine
Abstract
Burkholderia pseudomallei, and other members of the Burkholderia, are among the most antibiotic-resi (show more...)
EV-METRIC
22% (71st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
NAY
Focus vesicles
OMV
Separation protocol
Separation protocol
(d)(U)C
DG Filtration UF Protein markers
EV: CPS/ LPS
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Burkholderia pseudomallei
Sample Type
Bacteria
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
20
Density gradient
Lowest density fraction
20
Highest density fraction
40
Orientation
Bottom-up
Speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
LPS/ CPS
ELISA
Antibody details provided?
No
Detected EV-associated proteins
LPS/ CPS
Characterization: Particle analysis
EM
EM-type
cryo EM
Image type
Close-up
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EV-TRACK ID | EV110052 |
---|---|
species | Burkholderia pseudomallei |
sample type | Bacteria |
condition | NAY |
separation protocol | (d)(U)C DG Filtration UF |
Exp. nr. | 1 |
EV-METRIC % | 22 |